AAPS PHARMSCITECH
·
2026
·Vol. 27
·期 4
·198
·
IF 5.1
Zhou Ziling,Chen Xian,Liu Yuxin,Zheng Rongjiao,Huang Junxiang,Pan Xin,Wu Chuanbin,Jiang Junhuang
Besides improving drug solubility and stability, co-amorphous systems (COAMS) have recently been reported to enhance the pulmonary delivery efficiency of dry powder inhalation (DPI), offering a novel approach to the development of DPI. Conventional drug formulation development utilizes trial-and-error experiments, which require a laborious workload and resources. Moreover, the correlation of forming COAMS to enhanced pulmonary deposition remains underrepresented. Therefore, we proposed applying multiple machine learning (ML) models to the development of co-amorphous DPIs. In this study, we first constructed the database of COAMS through literature mining and then preprocessed the dataset with a molecular representation method. Subsequently, we successfully developed and evaluated the predictive performance of multiple ML models (i.e., logistic regression, random forests, XGBoost, LightGBM, and support vector machines) for forming a co-amorphous system. The five ML models' performance varied, yet all achieved satisfactory predictive accuracy (ACC) of around 0.80 in the testing subset. Specifically, LightGBM exhibited the highest ACC of 0.790 in cross-validation and 0.845 in the testing subset. In addition, SHapley Additive ex Planations (SHAP) analysis revealed that several molecular features (i.e., API_EState_VSA10, Co-former_BCUT2D_MRHI) are critical for models' prediction. More importantly, we conducted experimental validation by using salbutamol sulfate and indomethacin as model drugs to prepare co-amorphous DPIs based on the fine-tuned LightGBM model. The co-amorphous DPIs showed satisfactory aerodynamic performance with fine particle fractions of 41.87%—69.30%. In conclusion, we successfully demonstrated the feasibility of ML for guiding the formation of co-amorphous DPIs, further facilitating the development process in the future. Graphical
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高影响因子
Science China-Materials
·
2026
·Vol. 69
·期 6
·3312-3323
·
IF 7.7
Yin Ruize,Liu Juan,Zhang Xiaohui,Wang Peinan,Lu Jiajie,Xiong Weiwei,Yuan Saisai,Zheng Fenfen,Zhang Junhao,Zhu Wenlei
Amorphous metal-organic frameworks (aMOFs), owing to their abundant defects and unsaturated coordination sites, have emerged as ideal precursors for investigating electrocatalytic reconstruction mechanisms. However, a systematic understanding of how different modulation strategies affect the reconstruction pathways of amorphous MOFs and thereby determine the nature of the final active species remains lacking. In this study, an amorphous MOF constructed from 3,4,9,10-pyrene-tetracarboxylic acid (PTA) was employed as a controllable precursor platform to systematically compare two classical strategies (doping and alloying) in terms of their effects on structural reconstruction and oxygen evolution reaction (OER) performance. The results reveal that doping promotes preferential reconstruction into Fe-rich (oxy)hydroxides with more exposed active sites, whereas alloying tends to yield Fe-Co mixed (oxy)hydroxides with limited site exposure. Benefiting from this difference, the doped system FeCo 0.05 -PTA exhibits outstanding OER activity under alkaline conditions, with overpotentials of only 208 and 248 mV at 50 and 100 mA cm −2 , respectively, and a low Tafel slope of 36.2 mV dec −1 . In situ Fourier transform infrared spectroscopy (FTIR) captures the OOH* intermediate, confirming that the reaction follows the adsorbate evolution mechanism; density functional theory (DFT) calculations further demonstrate that the doped system possesses the lowest free-energy barrier (Δ G = 0.59 eV) at the rate-determining step. This study underscores the decisive role of precursor design in this system, elucidates the distinct effects of doping and alloying on the reconstruction pathways and final properties of amorphous MOF-derived (oxy)hydroxides, and provides valuable insights for the design and mechanistic understanding of related electrocatalysts.
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MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 7
·491
·
IF 5.4
Chen Shurui,Weng Jiachen,Chang Hao,Xiao Kaiming,Wu Yaling,Lu Yudong,You Ruiyun
A high-performance, flexible surface-enhanced Raman scattering (SERS) sensing platform designed to detect key hyperuricemia (HUA) biomarkers in non-invasive biofluids is presented. The platform employs a bimetallic nanocomposite hydrogel fabricated via a semi-interpenetrating polymer network, wherein hydroxyethyl cellulose-stabilized gold nanorods are integrated with in situ–reduced silver nanoparticles from oxidized sodium alginate within a sodium alginate/polyacrylamide hybrid network. The hydrogel SERS substrate exhibits outstanding mechanical properties with a tensile strain of up to 911.37% and excellent swelling behavior, achieving equilibrium swelling ratios of 1919.74% in deionized water and 1822.97% in artificial urine. It achieves detection limits of 2.09 × 10 − 10 M for uric acid, 1.70 × 10 − 10 M for xanthine, and 2.14 × 10 − 9 M for hypoxanthine. Spike-and-recovery experiments in artificial urine confirm recoveries consistently above 90%, validating the substrate’s excellent accuracy and reproducibility in complex biological matrices. Collectively, this work provides a novel and reliable approach for advancing non-invasive, real-time monitoring of HUA. Graphical abstract
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Advanced Fiber Materials
·
2026
·
IF 22.6
Xiao Lingfei,Huang Huayi,Ding Yunxiang,Lei Ming,Lei Qingjian,Yan Feifei,Shi Chaoran,Xu Jianbin,Cai Lin,Li Wanli
Extracellular vesicles (EVs) represent promising cell-free therapeutics for the regeneration of aged bone defects. Mechanical stimulation is critical in bone regeneration and significantly influences both EV secretion and cargo composition. Despite this, translating mechanically programmed EVs to clinically relevant doses remains a significant challenge. Most current scalable bioreactor systems has achieved high yields through high-density cell culture and fluid flow, yet they do not provide programmable or spatially consistent mechanical stimulation. In this study, an electrospun scaffold-enabled bioreactor was developed to integrate high-density three-dimensional macrophage culture with programmable cyclic stretching, enabling simultaneous EV scale-up and cargo programming. The interconnected fiber junction network transmits boundary-applied strain with enhanced spatial consistency, while the highly porous scaffold supports dense cell seeding and efficient EV release and collection. This approach increases EV yield by 13.8-fold compared to conventional two-dimensional culture. Fiber-mediated mechanical conditioning activates Piezo1-dependent Ca 2+ signaling and integrin-associated mechanotransduction, promotes Yes-associated protein (YAP) nuclear translocation, and remodels EV cargo composition. The resulting mechanically stimulated EVs (ms-EVs) demonstrate concurrent pro-regenerative activities, including cellular rejuvenation, vascular repair, and osteogenic enhancement, and perform favorably compared to canonical interleukin-4 (IL-4)-polarized M2 macrophage-derived extracellular vesicles (M2-EVs). In aged rat femoral defects, ms-EV-functionalized scaffolds increase bone volume fraction by approximately 1.7-fold at 8 weeks, reduce p16 + senescent cells, and enhance CD31 + neovascularization. These findings establish electrospun scaffolds as scalable mechanobiological manufacturing substrates for the production of functionally programmed EV therapeutics. Graphical
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Nature Communications
·
2026
·Vol. 17
·期 1
·5423
·
IF 18.1
Mo Xiaoyun,Huang Yongjie,Chen Haimin,Huang Junwen,Huang Kui,Zhao Suwen,Wang Xufei,Zhang Kuibo,Li Yang
The use of immunosuppressive therapies for rheumatoid arthritis (RA) is associated with substantial adverse effects. In addition, existing diagnostic reagents are limited in their ability to detect local joint damage. Here, we address both these needs using a collagen-hybridizing peptide (CHP) that selectively binds denatured collagen present in inflamed joints in RA models. Light-sheet scanning and immunohistology of the inflamed ankles in RA mice pre-injected with fluorescently-labeled-CHP revealed specific CHP accumulation within matrices of the synovial pannus and cartilage that express matrix metalloproteases and cathepsin K extensively. In vivo fluorescence probing with labeled CHP enabled dynamic monitoring of collagen breakdown throughout RA progression or regression with sensitivity beyond arthritis scoring. Moreover, bioconjugation of a therapeutic monoclonal antibody (mAb) against TNF with CHP enriched the mAb precisely within the pannus of inflamed joints in RA mice, resulting in improved efficacy relative to the native mAb and reduced systemic exposure. These findings highlight denatured collagen as a molecular matrix signature of RA lesions for diagnostic imaging and an effective target for RA drug delivery.
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Advanced Biotechnology
·
2026
·Vol. 4
·期 2
·21
·
IF 8.8
Chen Ruiqi,Zhou Zhenkun,Wang Jing,Zhang Yilin,Li Peibo,Wu Hao
Airborne particulate matter, particularly diesel particulate matter (DPM), is a major component of air pollution and poses significant risks to lung health. Tissue-resident alveolar macrophages (TR-AMs), which dominate the immune landscape of the alveoli, are essential for immune surveillance and pulmonary surfactant homeostasis. While previous studies have reported detrimental effects of particulate matter on macrophages, little effort has been made to its impact on the key physiological functions—chemotaxis and phagocytosis—of TR-AMs, the most predominant macrophage lineage in the lung. We found that DPM exposure markedly altered gene expression profiling in murine TR-AM cell line MH-S, notably downregulating genes related to cytoskeleton actin dynamics, chemotaxis, and bacterial recognition. Both MH-S cells and primary TR-AMs displayed impaired migration and phagocytosis of Escherichia coli and Staphylococcus aureus , associated with reduced filamentous actin (F-actin) polymerization and filopodia formation. Cytochalasin D treatment further confirmed the role of actin remodeling in bacterial engulfment. In vivo , DPM exposure reduced the proportion of highly phagocytic TR-AMs, accompanied by inefficient clearance of pulmonary surfactant, leading to accumulation of Periodic acid-Schiff-positive granules, elevated surfactant protein D, and excessive lipids in both alveoli and bronchoalveolar lavage fluid (BALF). Further compositional analysis of BALF revealed excessive accumulation of phosphatidylcholine, ceramide, and cholesterol in DPM-exposed mice. In conclusion, DPM disrupts F-actin–dependent migration and phagocytosis in TR-AMs, undermining alveolar immune surveillance and surfactant homeostasis. These results highlight a key mechanism by which air pollution compromises lung defense, offering insight into particulate matter–related respiratory disease pathogenesis.
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高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2026
·Vol. 37
·期 17
·1347
·
IF 3.2
Geng Zhihao,Yang Yujie,Ding Hongyu,Hu Tan,Ge Shaofan,Wang Xiyan,Tang Ying
The rapid proliferation of electromagnetic technology has led to severe electromagnetic pollution, driving an urgent need for efficient microwave-absorbing materials. Nickel-zinc ferrites are widely studied for this purpose; however, their inherent impedance mismatch, resulting from the large disparity between complex permeability and permittivity, leads to strong surface reflection and severely limits absorption efficiency. This study systematically investigates the regulatory effect of indium ion (In 3+ ) doping on the microwave absorption performance of spinel-type nickel‑zinc ferrites. First, Ni 0.48 Zn 0.32 Cu 0.2 Cr 0.1 In x Fe 1.9-x O 4 (x = 0, 0.1, 0.2, 0.3) ferrite powders were synthesized via a solid‑state reaction method, with the optimal doping level identified as x = 0.1. Subsequently, using this optimal composition, a series of composite materials with ferrite to β-SiC mass ratios of 9:1, 8:2, and 7:3 was prepared via high-energy ball milling. The introduction of non‑magnetic SiC aimed to achieve magnetic dilution, thereby optimizing the impedance matching and loss characteristics of the composites. Phase and morphological characterizations confirmed that all samples exhibited a pure spinel structure, with both phases uniformly distributed in the composites. Scanning electron microscopy revealed that the ferrite particles displayed regular morphology, with sizes ranging from 3 to 3.5 µm, and were well‑dispersed and mechanically integrated with the SiC particles. Electromagnetic measurements indicated that appropriate In 3+ doping (x = 0.1) improved the minimum reflection loss from -18.76 dB to -19.84 dB. Further composite formation with SiC led to a remarkable enhancement in absorption performance: at the optimal ratio (9:1), the composite achieved a significantly lower minimum reflection loss of ‑35.49 dB at 7.52 GHz, along with a broadening of the effective absorption bandwidth (RL ≤ -10 dB) from 4.0 GHz to 4.4 GHz. This work demonstrates that the absorption enhancement in the In-doped ferrite/SiC composite originates solely from the increased attenuation constant driven by boosted dielectric loss, providing a practical design paradigm for high-performance ferrite‑based microwave absorbers. These findings provide clear experimental evidence and mechanistic insights for the design of high‑performance ferrite‑based composite microwave absorbers.
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APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2026
·Vol. 110
·期 1
·156
·
IF 4.7
Xu Haoyue,Zhu Xueliang,Li Qian,Liu Yuxi,Wang Qi,Meng Xuelian
Peste des petits ruminants (PPR) is an acute, highly contagious viral disease caused by Peste des petits ruminants virus (PPRV). PPR has inflicted severe economic losses on livestock industries across numerous countries in Africa, Asia, and Europe, posing a significant threat to the survival of endangered wild ruminant species. In this study, we performed prokaryotic expression of the PPRV nucleocapsid (N) protein and prepared rabbit-derived polyclonal antibodies (pAbs). We also identified the characteristics of two mouse-derived monoclonal antibodies (mAbs) against the PPRV N protein. The selected mAb was labeled with horseradish peroxidase (HRP). A double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) was established to detect PPRV antigen using rabbit-derived pAbs and HRP-conjugated mAbs. The threshold for the DAS-ELISA was determined to be 0.227. The DAS-ELISA demonstrated minimum detection limits of 0.195 μg/mL for the recombinant PPRV N protein and 250 50% tissue culture infectious dose (TCID 50 ) for PPRV. Of note, the assay exhibited no cross-reactivity with Capripoxvirus (CaPV), Orf virus (ORFV), Lumpy skin disease virus (LSDV), Foot-and-mouth disease virus (FMDV), or Parainfluenza virus 5 (PIV5). The repeatability of the DAS-ELISA was validated with both intra-assay and inter-assay coefficients of variation (CV%) being less than 9%, indicating good repeatability. Compared to the results obtained using the commercial ID Vet kit (ID-Vet, Montpellier, France) for PPRV antigen detection on the 240 tissue samples, the DAS-ELISA showed a concordance rate of 93.75% and a kappa value of 0.86, demonstrating the reliability of the developed method. The results showed that the DAS-ELISA established in this study is a reliable diagnostic method for the PPRV detection. Key points • Polyclonal and monoclonal antibodies against PPRV N protein were prepared. • A DAS-ELISA based on mAbs and pAbs for detecting virus infection was developed. • The established DAS-ELISA exhibits excellent sensitivity and specificity.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2026
·
IF 15
Guo Ningli,Shi Jiayi,Zhang Xinyu,Xu Hui,Ji Tongyao,Qiu Fengkai,Li Kai,Wu Zhenyi,Ma Ji,Weng Qiaoyou,Qiu Rongfang,Shu Gaofeng,Chen Zhang,Chen Jingfeng,Wang Xiaojing
Inflammatory bowel disease (IBD) is a persistent intestinal inflammation driven by epithelial barrier dysfunction, immunological imbalance, and microbial dysbiosis. The restoration of gut homeostasis through biomaterial-based strategies offers a potential avenue for alleviating inflammation in IBD. Herein, an alginate-based hydrogel incorporating astaxanthin-loaded mesoporous catechin nanoparticles (AST@MCN-Gel) was designed to relieve inflammation and rebalance gut homeostasis. The mesoporous framework of MCN enabled effective encapsulation of the hydrophobic AST, thereby increasing its water solubility and bioavailability. In lipopolysaccharide-induced macrophages, AST@MCN exhibited superior ROS-scavenging capacity and anti-inflammatory activity relative to either AST or MCN. Mechanistically, AST@MCN modulated macrophage phenotype from pro-inflammatory (M1) to anti-inflammatory (M2) state through regulation of the MAPK/NF-κB pathway. On the other hand, the alginate-based hydrogel effectively enhanced the intestinal retention of AST@MCN, resulting in a marked improvement in oral therapeutic efficacy. In a DSS-induced IBD mouse model, oral administration of AST@MCN-Gel significantly alleviated the clinical symptoms and restored intestinal barrier integrity. Analysis of the gut microbiome further indicated that AST@MCN-Gel ameliorated gut dysbiosis by restoring microbial diversity and suppressing the proliferation of pathogenic species. Collectively, these underscore the potential of AST@MCN-Gel as an effective biomaterial-based therapeutic approach for restoring intestinal homeostasis and improving IBD therapy.
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高影响因子
BIOLOGICAL RESEARCH
·
2026
·
IF 5.4
Ying Shen,Xiaolin Gao,Jiashi Wang,Yingying Hao
Background: Abnormal syncytialization of cytotrophoblast (CTB) cells is known to be associated with preeclampsia (PE), however, the underlying molecular mechanisms remain elusive. Results: In this study, proteomic analysis of placental tissues was performed to identify proteins involved in PE pathogenesis. NCK Adaptor Protein 1 (NCK1) was found to be downregulated in placental tissues from patients with early-onset PE. In vitro functional assays revealed that NCK1 was markedly increased during spontaneous syncytialization of CTB cells. NCK1 overexpression alleviated hypoxia-induced defects in CTB cell fusion. Mechanistically, NCK1 upregulated both the expression and stability of Peroxisome Proliferator-Activated Receptor Gamma (PPARG), accompanied by increased m6A modification of its mRNA. Overexpression of PPARG significantly rescued hypoxia-induced syncytialization impairment, whereas PPARG deficiency abolished the promotive effects of NCK1 on CTB syncytialization. Additionally, phosphorylation of Eukaryotic Translation Initiation Factor 3 Subunit (EIF3D) was significantly increased during spontaneous syncytialization, and NCK1 overexpression partially reversed hypoxia-induced suppression of EIF3D phosphorylation. EIF3D knockdown significantly reduced m6A levels on PPARG mRNA, likely due to impaired NCK1-mediated suppression of Alpha-Ketoglutarate-Dependent Homolog 5 (ALKBH5), an m6A demethylase. Furthermore, NCK1 enhanced EIF3D phosphorylation by inhibiting its O-GlcNAcylation. Conclusions: Our findings demonstrate that NCK1 stabilizes PPARG by modulating the crosstalk between O-GlcNAcylation and phosphorylation of EIF3D, thereby restoring hypoxia-impaired syncytialization in CTB cells. This study identifies the NCK1/EIF3D/PPARG axis as a molecular pathway potentially relevant to trophoblast fusion dysfunction in PE.
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高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2026
·
IF 4.4
Cai Jingjing,Chen Zhenjie,Li Shenglong,Liu Haijun,Sun Tianyang,Chen Liwei,Jin Guanhua
The rational design of biomass-derived carbon aerogels is crucial for developing high-energy-density supercapacitors. Herein, we report a one-pot synthesis of in situ N/O dual-doped porous carbon aerogels from cotton fibers, utilizing a NaOH/urea aqueous system as a multifunctional solvent, followed by freeze-drying and carbonization. The optimized cotton fiber-derived carbon aerogel (HCFA-600) possesses a high specific surface area (398.4 m 2 g −1 ), a honeycomb-like pore architecture, and significant heteroatom doping. When tested in a three-electrode system, the HCFA-600 electrode achieves a notable specific capacitance of 249 F g −1 at 1 A g −1 . A symmetric coin-type supercapacitor (HCFA-600//HCFA-600) assembled using this material delivers a high energy density of 14.5 Wh kg −1 at a power density of 500 W kg −1 , while retaining 75.58% of its initial capacitance after 5,000 cycles at 5 A g −1 . The proposed synthesis route, which enables in situ heteroatom doping of porous carbon aerogels from renewable cotton fibers, is not only simple, eco-friendly, and cost-effective but also holds promising potential for application in high-performance supercapacitors.
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Chinese Journal of Integrative Medicine
·
2026
·
IF 3.1
Wang Ya-fen,Yue Shi-jun,Li Jia-yao,Meng Si-tong,Zheng Min,Zhang Yu-fang,Gu Ying,Hou Bin,Biao Ya-ning
Objective To explore the molecular mechanism of Xuefu Zhuyu Decoction (XFZYD) against myocardial fibrosis. Methods The active components of XFZYD were identified by high performance liquid chromatography (HPLC). Network pharmacology, molecular docking, and affinity dot-mass spectrometry (AD-MS) were used to predict and validate the binding interaction between XFZYD components and copper transporter 1 (CTR1). Sixty male Kunming mice were randomly divided into 6 groups using a random number table. Control, isoproterenol hydrochloride (ISO), captopril, low-, medium-, and high-dose XFZYD (5.69, 11.38, and 22.76 g/kg) groups, with 10 mice in each group. Except the control group, mice received daily subcutaneous injection of ISO (10 mg/kg) for 28 d to induce myocardial fibrosis, concurrently treated with respective drugs by gavage. Cardiac function was assessed by echocardiography (ECG) and electrocardiography (UCG). Serum myocardial injury markers and transforming growth factor beta 1 (TGF-β 1) were measured by a biochemical analyzer and enzyme-linked Immunosorbent assay (ELISA). Pathological changes and collagen deposition was observed using HE, Masson and sirius red staining methods. Copper content in cardiac tissue was determined by inductively coupled plasma mass spectrometry (ICP-MS), and protein expressions of collagen I, III, alpha-smooth muscle actin (α-SMA), lysyl oxidase (LOX), CTR1, copper chaperone for superoxide dismutase (CCS), and cytochrome c oxidase 17 (COX17) were detected by Western blot, immunohistochemistry, and immunofluorescence. In vitro , TGF-β 1 (5 ng/mL)-stimulated NIH-3T3 fibroblasts were treated with XFZYD (60 mg/L), with or without CuCl 2 (20 µ mol/L) or SLC31A1 overexpression for 24 h, and the same indicators were evaluated. Results HPLC identified 7 major components in XFZYD. Network pharmacology revealed that XFZYD targets copper metabolism related genes. Molecular docking showed strong binding affinities between the 7 compounds and CTR1, and AD-MS confirmed direct binding of paeoniflorin and amygdalin to CTR1. In vivo , XFZYD could ameliorate the collagen deposition, cardiac dysfunction, and elevated levels of collagen I/III, α-SMA, LOX, and copper content exhibited in ISO-induced myocardial fibrosis mice ( P <0.05 or P <0.01). In vitro , XFZYD had the effects of suppressing copper overload and the upregulation of LOX, collagen I/III, and α-SMA in TGF β 1 stimulated NIH-3T3 fibroblasts, whereas co-treatment with CuCl 2 exacerbates these fibrotic responses and attenuates the protective effect of XFZYD ( P <0.05 or P <0.01). XFZYD had the effect of partially reversing the enhanced copper accumulation, LOX activation, and collagen deposition induced by SLC31A1 (CTR1) overexpression in TGF β 1 stimulated fibroblasts, and SLC31A1 overexpression reduces its efficacy ( P <0.05 or P <0.01). Conclusions XFZYD exerted a protective effect against ISO-induced myocardial fibrosis. The mechanism may be related to regulating the CTR1–copper–LOX axis, thereby reducing excessive collagen deposition and cross-linking in cardiac fibroblasts.
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