NEUROCHEMICAL RESEARCH
·
2022
·Vol. 48
·期 2
·570-578
·
IF 4.414
Guo Shenglong,Lei Qi,Guo Hena,Yang Qian,Xue Yanli,Chen Ruili
Ferroptosis and neuroinflammation play a crucial role in the pathogenesis of Alzheimer’s disease (AD), and Edaravone (EDA) has been demonstrated to have anti-inflammatory, antioxidant and neuroprotective effects in neurodegenerative diseases. However, the relationship between EDA and ferroptosis in AD is unidentified. This research aimed to elucidate the mechanism of EDA in AD with Aβ 1-42-induced HT22 cells as in vitro cell model. The results showed that EDA could significantly reduce Aβ 1-42 -induced apoptosis of HT22 cells and formation of pro-inflammatory factors TNF-α, IL-1β and IL-6, prevent the activation of TLR4/NF-κB /NLRP3 signaling pathway, and inhibit ferroptosis and lipid peroxidation. Taken together, EDA contributes to inhibiting neuroinflammatory injury and ferroptosis in Aβ 1-42-induced HT22 cells, and thus may be a potential candidate for the treatment of AD.
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BIODEGRADATION
·
2022
·Vol. 34
·期 1
·73-81
·
IF 3.731
Gu Chen,Liang Jinxuan,Liu Ming,Rui Jianliang,Shi Jiyan,Yu Yanming,Zhang Xuwang
Bisphenol A (BPA) has been widely used in the manufacture of polymeric materials. BPA is regarded as an endocrine disrupting chemical, posing a great threat to the public health. In this study, a bacterial strain LM-1, capable of utilizing BPA as the sole carbon and energy source under aerobic conditions, was originally isolated from an activated sludge sample. The isolate was identified as Pseudomonas sp. based on 16S rRNA gene sequence analysis. Strain LM-1 was able to completely degrade 25–100 mg/L BPA within 14–24 h, and it also exhibited high capacity for BPA degradation at a range of pH (6.0–8.0). (NH 4 ) 2 SO 4 and NH 4 NO 3 were the suitable nitrogen sources for its growth and BPA biodegradation, and the BPA degradation could be accelerated when exogenous carbon sources were introduced as the co-substrates. Metal ions such as Zn 2+ , Cu 2+ , and Ni 2+ could considerably suppress the growth of strain LM-1 and BPA degradation. According to the analysis of liquid chromatography coupled to Q-Exactive high resolution mass spectrometry, hydroquinone, p -hydroxybenzaldehyde, and p -hydroxybenzoate were the predominate metabolites in the BPA biodegradation and the degradation pathways were proposed. This study is important for assessment of the fate of BPA in engineered and natural systems and possibly for designing bioremediation strategies.
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APPLIED CATALYSIS A-GENERAL
·
2022
·
IF 5.723
Yue Li,Huan Ren,Daolai Sun,Xinqiang Zhao,Satoshi Sato
Liquid-phase catalytic hydrodeoxygenation of 1,2-decanediol (1,2-DDO) to form 1-decanol was performed over various non-noble metal-loaded acidic catalysts, and Cu loaded on amorphous SiO 2 -Al 2 O 3 exhibited a high catalytic activity for the formation of 1-decanol. A high 1-decanol selectivity of 84.7% with a complete 1,2-DDO conversion was obtained within only 15 min over the Cu/SiO 2 -Al 2 O 3 at 250 °C under 10 MPa H 2 pressure. The temperature at 250 °C and the H 2 pressure higher than 3 MPa were conducive to the efficient production of 1-decanol from 1,2-DDO. In contrast to the previously reported noble metal catalysts, Cu/SiO 2 -Al 2 O 3 required a relatively high reaction temperature, while an extremely high 1-decanol productivity of 78 mmol g cat −1 h −1 could be achieved. Meanwhile, the hydrodeoxygenation of 1,2-decanediol over Cu/SiO 2 -Al 2 O 3 proceeded via a reaction mechanism in which decanal was formed as the intermediate. In addition, the Cu/SiO 2 -Al 2 O 3 catalyst was reusable at least five times without serious deactivation.
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Polymers
·
2022
·Vol. 14
·期 12
·2470
·
IF 4.967
Jing Yang,Xujiao Tang,Qingqing Wu,Panpan Ren,Yishu Yan,Wei Liu,Chun Pan
Heparin has shown benefits in severe acute pancreatitis (SAP) therapy, but the underlying mechanisms were unknown. Extracellular high-mobility group protein-1 (HMGB-1) has been regarded as a central mediator contributing to inflammation exacerbation and disease aggravation. We hypothesized heparin attenuated the disease by targeting HMGB-1-related pathways. In the present study, the possible therapeutic roles of heparin and its non-anticoagulant derivatives, 6-O-desulfulted heparin and N-acylated-heparin, were determined on mouse models induced by “Two-Hit” of L-arginine. The compounds exhibited potent efficiency by substantially decreasing the pancreatic necrosis, macrophage infiltration, and serum inflammatory cytokine (IL-6 and TNF-α) concentration. Moreover, they greatly reduced the rapidly increasing extracellular HMGB-1 levels in the L-arginine injured pancreases. As a result, multiple organ failure and mortality of the mice were inhibited. Furthermore, the drugs were incubated with the RAW264.7 cells activated with damaged pancreatic tissue of SAP mice in vitro. They were found to inhibit HMGB-1 transfer from the nucleus to the plasma, a critical step during HMGB-1 active secretion from macrophages. The results were carefully re-examined with a caerulein and LPS induced mouse model, and similar results were found. The paper demonstrated heparin alleviated SAP independent of the anti-coagulant functions. Therefore, non-anticoagulant heparin derivatives might become promising approaches to treat patients suffering from SAP.Keywords:heparin;severe acute pancreatitis;HMGB-1;macrophage
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Journal of Science-Advanced Materials and Devices
·
2022
·
IF 7.382
Xiaoyan Xue,Yan Sun,Qiwen Sun,Weiren Bao,Zongsen Zhang,Liping Chang,Jiancheng Wang,Kechang Xie
As an atom economy technology, the direct esterification reaction of olefins plays a significant role in the production of organic esters. In this work, a series of resin-supported HPW catalysts were prepared using D101, D152, RM027, and A15 as the carriers, and they were used to prepare 1-methylpentyl acetate by the esterification of 1-hexene and acetic acid. The composition, structure, morphology, acid content, and other parameters of catalysts were characterized. In the esterification reaction, the activity order of catalysts is as follows: 20.0%HPW/A15 > 20.0%HPW/D101 ≈ 20.0%HPW/D152 > 20.0%HPW/RM027. It depends on the acid strength of the carrier, the interaction between the carrier and HPW, and the HPW content. Moreover, it can be concluded that the weak acid resin carrier is completely inactive for the esterification reaction of 1-hexene, and the ratio of acetic acid to 1-hexene has a significant influence on the product selectivity. When n (acetic acid)/n (1-hexene) = 2, the optimum 1-methylpentyl acetate selectivity is 38.2%. Results of repeated experiments confirm that the stability of the active constituent is related to the type of functional groups on the carrier.
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CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Zhaohui Wu,Jihuai Wu,Shibo Wang,Chunyan Wang,Yitiani Du,Ying Wang,Jialian Geng,Yuhe Lin,Weihai Sun,Zhang Lan
The rapid development of perovskite solar cell (PSC) makes it a typical representative of the third generation PV technology. The large-scale manufacturing of the device still faces many challenges, such as enhancing efficiency and improving stability. The core problem is the suppression of various defects and the adjustment of energy levels among components. In this report, a multifunctional molecule of potassium nonafluoro-1-butanesulfonate (KFBS) is introduced into SnO 2 electron transport layer (ETL). Owing to the synergistic effect of potassium ion, sulfonic group and nonafluorobutane group in KFBS, the energy match between perovskite (PVK) and ETL is tuned, the PVK and ETL quality is improved, and the defects at interface, on grain boundaries and in bulk are passivated. Consequently, the power conversion efficiencies increase from 20.6% for pristine device to 23.21% for KFBS-modified device. Moreover, unpacked KFBS-modified device still maintains its initial efficiency of 90.5 % after storing at room temperature and relative humidity of 30 % in dark for 1000 h, showing an excellent environmental stability.
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MATERIALS & DESIGN
·
2022
·
IF 9.417
Lingling Pan,Dawei Jiang,Luqi Pan,Zhizhen Meng,Yangyang Zhuang,Yueyue Huang,Fanrong Ye,Changcan Shi,Jie Chen,Jingye Pan
Sepsis is defined as life-threatening organ dysfunction caused by a dysregulated host response to infection and causes high ratios of morbidity and mortality worldwide. Although many potential therapeutic targets have been reported, the therapeutic effect needs to be further improved. Herein, we report a strategy to specifically target inflammation site via anti-ICAM-1 antibody bonded antibacterial peptides polymeric nanoparticle. Antibacterial peptide (KR-12) grafted amphipathic PCL- b -P(OEGMA- co -AMA)- g -Cys-KR-12 block copolymer and PCL- b -P(OEGMA- co -AMA)- g -biotin block copolymer were synthesized by ring-opening polymerization and click chemistry. Then, antibacterial peptides polymeric nanoparticles (AMPNP) were prepared by co-assembly of amphipathic PCL- b -P(OEGMA- co -AMA)- g -Cys-KR-12 and PCL- b -P(OEGMA- co -AMA)- g -biotin in aqueous solution. Intercellular adhesion molecule-1 (ICAM-1) is highly expressed on the surface of inflammatory endothelial cells, and the anti-ICAM-1 antibody can specifically bind to it. To obtain the anti-ICAM-1-AMPNP nanoparticles, biotinylated anti-ICAM-1 antibodies were bonded on the surface of AMPNP nanoparticles through the biotin-avidin system. The anti-ICAM-1-AMPNP nanoparticles had low systemic toxicity and exhibited ideal antibacterial property against drug-resistant bacteria, Gram-negative bacteria and Gram-positive bacteria. The anti-ICAM-1-AMPNP nanoparticles exhibited favorable targeting capabilities to inflammation endothelial cells in vitro and significantly reduced the inflammatory response of CLP mice in vivo . The present investigation might provide a specific strategy for targeting combating sepsis with anti-ICAM-1-AMPNP nanoparticles.
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Advanced Healthcare Materials
·
2023
·
IF 10
Jian Tang,Yang Liu,Mingxi Li,Xiao Wang,Anning Du,Ning Gu,Fang Yang
Vascular inflammation is an early manifestation and common pathophysiological basis of numerous cardiovascular and cerebrovascular diseases. However, effective surveillance methods are lacking. In this study, sulfur hexafluoride (SF 6 )-loaded polylactic acid- co -glycolic acid (PLGA) nanobubbles (NBs) with a surface assembly of cyclodextrin (CD) and sphingosine-1-phosphate (S1P) (S1P@CD-PLGA NBs) are designed. The characterization results show that S1P@CD-PLGA NBs with diameters of ≈200 nm have good stability, biosafety, and ultrasound imaging-enhancement effects. When interacting with inflammatory vascular endothelial cells, S1P molecules encapsulated in cyclodextrin cavities exhibit a rapid, excellent, and stable targeting effect owing to their specific interaction with the highly expressed S1P receptor 1 (S1PR1) on the inflammatory vascular endothelial cells. Particularly, the S1P–S1PR1 interaction further activates the downstream signaling pathway of S1PR1 to reduce the expression of tumor necrosis factor-α (TNF-α) to protect endothelial cells. Furthermore, mouse models of carotid endothelial injuries and mesenteric thrombosis demonstrate that S1P@CD-PLGA NBs have excellent capabilities for in vivo targeting imaging. In summary, this study proposes a new strategy of using S1P to target inflammatory vascular endothelial cells while reducing the expression of TNF-α, which has the potential to be utilized in the targeted surveillance and treatment of vascular inflammatory diseases.
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Journal of Flow Chemistry
·
2023
·Vol. 13
·期 4
·375-383
·
IF 2.7
Lai Liangchuan,Gao Liang,Liu Minjie,Guo Yongxing,Cheng Dang,Jiang Meifen,Chen Fener
Continuous flow technology has been widely adopted in manufacturing active pharmaceutical ingredients (APIs). Herein, we report an expeditious multi-step continuous-flow strategy for an efficient and highly productive flow synthesis of 7-methoxy-1-tetralone, which is an essential intermediate for the opioid analgesic drug (-)-dezocine. Compared with the traditional batch operation, this work presents significant advantages of continuous-flow chemistry with dramatically reduced reaction time, highly improved reaction efficiency, good controls over reaction optimizing conditions, etc. The flow protocol in this work provided the desired product in an overall yield of up to 76.6% with 99% purity, much higher than those from batch process (i.e., 50% yield, 92% purity). Moreover, reaction efficiency is highly improved with a throughput of 0.49 g/h, the total reaction time is markedly reduced from hours in batch to minutes in flow process. Graphical abstract
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SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Yingli Han,Yuehua Wang,Fangyu Zhou,Yuyang Jiang,Feng Liu
Human apurine/apyrimidine endonuclease (APE) is a multifunctional enzyme that affects the occurrence and development of cancer through DNA repair and redox regulation. It has been identified as a tumor marker for detection and prognosis and drug target due to its overexpression in a variety of cancers. However, the accurate detection of APE 1 activity is still a huge challenge. Traditional approaches exist some disadvantages, including low sensitivity, complex operation and professional equipment requirements. Therefore, it is necessary to develop a fast, sensitive and simple biosensor to detect APE 1 activity in vivo and in vitro. In this report, a new tetrahedral molecular beacon (TMB) probe is designed for the detection of APE 1 activity. This probe not only has the advantage that the tetrahedron can enter the cells without a transfection agent, but also has the advantages of simple operation and low cost. The extracellular detection limit of TMB is as low as 0.0026 U/mL, and this program has successfully achieved APE 1 intracellular imaging to distinguish cancer cells overexpressing APE 1 from normal cells. In addition, the probe which is an effective mean for screening APE 1 inhibitor can also monitor the response of APE 1 to different drugs. All in all, our probe can be successfully applied to detect APE 1 activity in vivo and vitro, and can achieve living cells drug screening. And this probe also provides a general method for the detection of other endogenous biological targets in living cells.
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APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING
·
2021
·Vol. 127
·期 12
·1-13
·
IF 2.584
Li Wenchuan,Wu Heng,Ao Hong,Zeng Zhixin,Gao Rongli,Cai Wei,Fu Chunlin,Deng Xiaoling,Chen Gang,Wang Zhenhua,Lei Xiang
Multiferroic composite ceramics x Ni 0.15 Cu 0.25 Zn 0.6 Fe 2 O 4 -(1- x ) Ba 0.85 Ca 0.15 Zr 0.1 Ti 0.9 O 3 ( x = 0, 0.2, 0.3, 0.4, 0.5, 0.6, 0.9 and 1) were prepared by combining chemical co-precipitation method with sol–gel method; the microstructure, dielectric, ferroelectric, magnetic and multiferroic properties were comparatively investigated. XRD results show that all the specimens have obvious bi-phases structure. The grains with larger size are magnetic phase Ni 0.15 Cu 0.25 Zn 0.6 Fe 2 O 4 (NCZF), while that of smaller size can be attributed to ferroelectric phase Ba 0.85 Ca 0.15 Zr 0.1 Ti 0.9 O 3 (BCZT). The dielectric constant of the sample with x = 0.6 is the largest at low frequency but the specimen x = 0.2 has the highest value of dielectric constant in high-frequency region. When x = 0.9, the ceramic has the largest loss, while it presents the lowest loss value when x = 0.3. The height of the peak decreases with increase in the frequency, and the position of the peak moves to higher temperature range with the decrease in x . The dielectric loss increases sharply with temperature, especially when x = 0.9, when the temperature is higher than 300 °C, the dielectric loss reaches hundreds of times under low frequency. Al samples present apparent ferroelectric hysteresis loops, but further characterization shows that the hysteresis may be attributed to leakage current. The remnant polarization( P r ) and coercive field( E c ) do not monotonically change with x , P r is 0.1576 μC/cm 2 at 2 kHz when x = 0.6. The magnetization monotonically changes with the x , indicating that there is a strong interfacial interaction between the two phases. Under the action of an external magnetic field of 1 mT, the magnetoelectric (ME) coupling is the strongest (relative polarization change 37%) when x = 0.2.
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MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 12
·1-8
·
IF 5.479
Meng Xiangying,Chen Xiao,Wu Weihua,Zheng Wei,Deng Haohua,Xu Luyao,Chen Wei,Li Zhulai,Peng Huaping
Molybdenum oxide quantum dots (MoO x QDs) were synthesized by a one-pot method and used as a versatile probe in an electrochemiluminescent (ECL) immunoassay of the non-small cell lung cancer biomarker cytokeratin 19 fragment 21–1 (CYFRA21-1) as a model analyte. The MoO x QDs exhibited stable and strong cathodic green ECL, with an emission peak at 535 nm, in the presence of K 2 S 2 O 8 within the potential range of −2.0 to 0 V. On exposure to CYFRA21-1, the ECL decreases because of the immunoreaction between CYFRA21-1 and its antibody which generates a barrier for electron transfer. The determination of CYFRA21-1 with favorable analytical performances was successfully realized under the optimal conditions. ECL decreases linearly in the 1 pg mL −1 to 350 ng mL −1 CYFRA21-1 concentration range, and the detection is as low as 0.3 pg mL −1 . Excellent recoveries from CYFRA21-1-spiked human serum indicate that the assay can be operated under physiological conditions.
Schematic representation of the fabrication of molybdenum oxide quantum dots (MoO x QDs) and the electrochemiluminescent (ECL) immunoassay based on the use of the MoO x QDs ECL probe for cytokeratin 19 fragment 21–1 (CYFRA21-1).
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