MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Nuo Chen,Zhiyuan Xing,Hongying Liu,Lu Fang,Yuqing Zhang,Lihua Li,Zheng-Zhi Yin,Maofeng Wang
Reduced nicotinamide adenine dinucleotide (NADH) plays crucial roles in maintaining cell growth and differentiation, cytoprotection and energy metabolism. And it is imperative to precisely monitor the variable quantity of NADH. Herein, the connotative feature of acupuncture needle (AN) was explored as electrochemical matrix. Poly-3, 4-ethylenedioxythiophene-liganded gold nanoparticle modified AN electrode (ANE/AuNPs/PEDOT) was prepared via a simple electrosynthesis technology. Working together by charge transfer and reaction has a strong response to the electron-transfer of “N” in NADH, which is coordinately presented as a current peak. Interestingly, the behavior of electron-transfer was affected by ambient environment. After the conditional optimization, the fabricated sensor exhibited good linear range of 20 ∼ 500 μmol/L with a detection limit of 18.5 μmol/L. In addition, the sensor can be successfully applied to analysis NADH in serum. Furthermore, the microelectrode exhibits the real-time monitor ability for NADH that released from MDA-MB231 cells. This sensor also possesses the significant performance of high selectivity, stability and reproducibility. Based on the acupuncture history, the developed ANE should be a fascinating microsensor besides the low price, fast response and user-friendly operation.
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TALANTA
·
2024
·
IF 5.6
Yuhua Chen,Yanghan Sun,Yi Li,Zhuo Wen,Xinyu Peng,Yuanke He,Yuanfang Hou,Jingchuan Fan,Guangchao Zang,Yuchan Zhang
To enhance personalized diabetes management, there is a critical need for non-invasive wearable electrochemical sensors made from flexible materials to enable continuous monitoring of sweat glucose levels. The main challenge lies in developing glucose sensors with superior electrochemical characteristics and high adaptability. Herein, we present a wearable sensor for non-enzymatic electrochemical glucose analysis. The sensor was synthesized using hydrothermal and one-pot preparation methods, incorporating gold nanoparticles (AuNPs) functionalized onto aminated multi-walled carbon nanotubes (AMWCNTs) as an efficient catalyst, and crosslinked with carboxylated styrene butadiene rubber (XSBR) and PEDOT:PSS. The sensors were then integrated onto screen-printed electrodes (SPEs) to create flexible glucose sensors (XSBR-PEDOT:PSS-AMWCNTs/AuNPs/SPE). Operating under neutral conditions, the sensor exhibits a linear range of 50 μmol/L to 600 μmol/L, with a limit of detection limit of 3.2 μmol/L (S/N = 3), enabling the detection of minute glucose concentrations . The flexible glucose sensor maintains functionality after 500 repetitions of bending at a 180° angle, without significant degradation in performance. Furthermore, the sensor exhibits exceptional stability, repeatability, and resistance to interference. Importantly, we successfully monitored changes in sweat glucose levels by applying screen-printed electrodes to human skin, with results consistent with normal physiological blood glucose fluctuations. This study details the fabrication of a wearable sensor characterized by ease of manufacture, remarkable flexibility, high sensitivity, and adaptability for non-invasive blood glucose monitoring through non-enzymatic electrochemical analysis . Thus, this streamlined fabrication process presents a novel approach for non-invasive, real-time blood glucose level monitoring.
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ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Zhen Wei,Yanxin Li,Shuyue Guo,Rui Han,Xiliang Luo
The accurate and reliable quantification of the levels of disease markers in human sweat is of significance for health monitoring through wearable sensing technology, but the sensors performed in real sweat always suffer from biofouling that cause performance degradation or even malfunction. We herein developed a wearable antifouling electrochemical sensor based on a novel multifunctional hydrogel for the detection of targets in sweat. The integration of polyethylene glycol (PEG) into the sulfobetaine methacrylate (SBMA) hydrogel results in a robust network structure characterized by abundant hydrophilic groups on its surface, significantly enhancing the PEG-SBMA hydrogel's antifouling and mechanical properties. The wearable sweat sensor is specifically designed for ascorbic acid (AA) detection. The incorporation of a silver nanoparticles-molybdenum disulfide (AgNPs-MoS 2 ) composite material into the hydrogel significantly enhances its catalytic properties towards AA. Electrochemical analysis confirms that the sensor reliably detects AA in real sweat with minimal interference from other components and bacteria, demonstrating its practical application potential. Furthermore, this multifunctional hydrogel's mechanical robustness and strong adhesion to various substrates ensure its practical applicability in wearable devices. This technology provides a foundation for accurate health monitoring in wearable sensors, enabling advanced, non-invasive diagnostic tools for personalized healthcare.
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ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Xuecui Mei,Lei Zhou,Liang Zhu,Bin Wang
Microfluidic chips play a crucial role in wearable sensors for sweat collection. However, previously reported wearable microfluidic chips, such as those based on poly(dimethylsiloxane) (PDMS) and paper, encounter sweat accumulation at the skin–sensor interface in practical applications, which consequently affects both sensing stability and wearing comfort. Herein, we propose a composite nanofiber membrane (CNMF)-based microfluidic chip for in situ sweat collection. The CNMF with directional water transport capability was integrated with patterned PDMS to prepare microfluidic chips. On one hand, sweat can be automatically transported to the analysis area along the designed pathway. On the other hand, sweat transfers from the hydrophobic membrane close to the skin to the hydrophilic membrane, effectively avoiding sweat accumulation and facilitating a comfortable skin microenvironment. Subsequently, we constructed a CNMF-based microfluidic fluorescence sensor for the analysis of multiple targets in human sweat. A portable 3D-printed device was employed for the visual signal output. Results indicated that the microfluidic sensor exhibits excellent reliability for collecting and analyzing sweat. This work provides new insights into the construction of wearable microfluidic chips with enhanced wearing comfort.
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NANOTECHNOLOGY
·
2024
·Vol. 35
·期 41
·415501
·
IF 2.9
Yanxin Liao,Shupei Sun,Qian Du,Nianfeng Shi,Guangfu Yin,Zhongbing Huang,Xiaoming Liao
Metal–organic frameworks (MOFs) are believed to be promising precursors for constructing novel and efficient catalysts for glucose sensing. Herein, HKUST-1 precursors are first fabricated using a one-pot hydrothermal approach, and then HKUST-1 is converted into porous Cu2S/CuO octahedrons through conformal sulfidation with the help of OH− ions. The as-obtained Cu2S/CuO composite can provide rich electrochemical active sites and promoted electric transfer kinetics. Benefiting from these combined merits, the as-fabricated Cu2S/CuO composite is confirmed to be a high-performance catalyst, with high sensitivities of 8269.45 and 4140.82 μA mM−1cm−2 in the corresponding ranges of 0.05 ∼ 0.6 mM and 0.6 ∼ 1.2 mM, respectively. Moreover, the as-prepared electrode materials possess good anti-interference ability, reproducibility and long-term stability. This work opens up new avenues for the design and preparation of transition metal sulfide composites.
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ChemistryOpen
·
2024
·
IF 2.5
Yuanyong Yao,Tao Wu,Meng Zhang,Daihua Fu,Hai Yang,Shixue Chen
Graphical The test method for assaying inhibitory effects of bioflavonoids on xanhine oxidase in vitro has been improved successfully with the equipment of UV-Vis technique. In the improved test method, there is a micro-environment surrounding XOD has been created in the presence of •O 2 - , which is similar to that of in vivo . Simultaneously, the inhibition of dihydromyricetin as a chiral flavonoid was effectively assayed by the improved test method, indicating dihydromyricetin enabling to be a potent inhibitor of XOD, resulting from the selective transformation of DMY to MY occurred. The difference on inhibitory effects of bioflavonoids inhibiting XOD activity assayed by varying test methods cause of us to be further in consideration. The reported test method creating a micro-environment surrounding XOD in the absence of ⋅O 2 − , which is seemly different from the assay in vivo . So, the vitro test method for assaying XOD activity is necessary to be improved for selection of potential inhibitors in the presence of ⋅O 2 − . The inhibitory results demonstrated that bioflavonoids of MY, DMY, QUE and LUT are capable to be on effective IC 50 values, but others are not. As well, their resulting inhibitions determined by the improved test method are much less than that reported in the literature, indicating that their chemical affinities with XOD become weaker. Moreover, DMY assayed on the inhibitions of XOD in the improved test method performs to be a better inhibitor, as compared to the assay of the reported test methods. Abasing on the transformation of DMY into MY in the presence of ⋅O 2 − , the good inhibition of DMY on XOD activity can be explained by the synergistic effect of MY.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Zhuoran Li,Limin Cao,Jianxin Sui,Lei Wang,Hong Lin,Kaiqiang Wang
The timely detection of freshness changes of aquatic products is crucial. In this study, we have developed a reliable, cost-effective, and user-friendly method for rapidly detecting hypoxanthine using a xanthine oxidase (XOD)/nanozyme enzymatic cascade system. The nanozyme, derived from the Fe 7 /Ni 3 metal–organic framework (Fe 7 Ni 3 MOF), exhibited good peroxidase-mimetic activity and stability. Our proposed XOD/Fe 7 Ni 3 MOF enzymatic cascade system demonstrated a linear response to hypoxanthine in the range of 3–70 μM, with a low detection limit of 1.39 μM. We also analyzed hypoxanthine in actual aquatic products, achieving spiked recoveries ranging from 90.04 % to 107.37 %. The correlation coefficient between our developed colorimetric method and the HPLC method was 0.98. Importantly, our proposed method holds several advantages over alternative techniques, particularly in terms of cost-effectiveness, precision, and speed. Consequently, this methodology shows great promise for the early detection of freshness changes in aquatic samples.
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LANGMUIR
·
2024
·
IF 3.7
Peng Lei,Shan Zhao,Muhammad Asif,Ayesha Aziz,Ying Zhou,Chuan Dong,Minglu Li,Shaomin Shuang
The presence of abnormal dopamine (DA) levels may cause serious neurological disorders, therefore, the quantitative analysis of DA and its related research are of great significance for ensuring health. Herein, the bovine serum albumin (BSA) template method has been proposed for the preparation of catalytically high-performance ruthenium dioxide/multiwalled carbon nanotube (RuO2/MWCNT) nanocomposites. The incorporation of MWCNTs has improved the active surface area and conductivity while effectively preventing the aggregation of RuO2 nanoparticles. The outstanding electrocatalytic performance of RuO2/MWCNTs has promoted the electro-oxidation of DA at neutral pH. The electrochemical sensing platform based on RuO2/MWCNTs has demonstrated a wide linear range (0.5 to 111.1 μM), low detection limit (0.167 μM), excellent selectivity, long-term stability, and good reproducibility for DA detection. The satisfactory recovery range of 94.7% to 103% exhibited by the proposed sensing podium in serum samples signifies its potential for analytical applications. The aforementioned results reveal that RuO2/MWCNT nanostructures hold promising aptitude in the electrochemical sensor to detect DA in real samples, further offering broad prospects in clinical and medical diagnosis.
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Biosensors-Basel
·
2025
·Vol. 15
·期 1
·49
·
IF 5.6
Yijiong Xue,Chengqi Bao,Hui Liu,Fanghui Ma,Minghui Yang,Xiaoqing Li
A fluorescence probe for “switch-on” detection of alkaline phosphatase (ALP) was developed based on Au nanoclusters anchored MnO2nanosheets (Au NCs-MnO2NSs), which were synthesized using bovine serum albumin (BSA) as template through a simple one-pot approach. In the sensing system, MnO2NSs function as both energy acceptors and target identifiers, effectively quenches the fluorescence of Au NCs via fluorescence resonance energy transfer (FRET). The presence of ALP catalyzes the hydrolysis of L-ascorbic acid-2-phosphate (AAP) to ascorbic acid (AA), reducing MnO2NSs to Mn2+and facilitate the fluorescence recovery of Au NCs. The fluorescence assay offers the advantages of facile preparation, cost-effectiveness, good specificity, and high sensitivity. Moreover, the assay exhibits a broad linear range (0.005 U/mL to 8 U/mL) for ALP detection with a remarkable limit of detection of 0.0015 U/mL. Notably, this assay demonstrates promising applicability for detection ALP in human serum samples, thereby providing valuable potential for clinical applications.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Jing Li,Jijie Shi,Hui Ren,Junjie Yang,Dan Xiao,Xiaoqin Li,Xinhui Wang,Yong Guo,Zhaoyu Jin
A novel non-enzymatic glucose sensor is developed by directly growing flower-shaped NiCo 2 O 4 microsphere on flexible carbon cloth (NCO/CC) through a one-step electro-deposition with subsequent mild heat treatment. Benefiting from the excellent conductivity between the three-dimensional (3D) structure of NiCo 2 O 4 and CC, as well as the synergic effect between the nickel and cobalt cations, the NCO/CC can not only offer more additional active sites, but also significantly enhance its electrocatalytic performance. Under the optimized conditions, the NCO/CC electrode reveals excellent activity with a broad linear detection range from 10 to 2000 μM, a high sensitivity of 5260 μA mM −1 cm −2 , and a low detection limit (LOD) of 0.2 μM, along with good repeatability and long-term stability. Furthermore, the NCO/CC was successfully utilized to detect glucose in actual beverage samples, showing competitive performance. The straightforward and practical method offers a novel approach to measuring glucose in real-world applications. Novelty statement 3D flower-shaped NiCo 2 O 4 microspheres growing on flexible carbon cloth (NCO/CC) were fabricated by a facile one-step electro-deposition with subsequent mild heat treatment. The fabricated NCO/CC electrode exhibits a wide linear detection range, high selectivity, good reproducibility, and stability towards the detection of glucose.
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Scientific Reports
·
2024
·Vol. 14
·期 1
·1-14
·
IF 3.8
Bian Lijun,Su Xinglin,Wang Jialu
Carbon cloth (CC) was electrochemically activated using three electrochemical methods in different electrolytes. The gases released during the activation process etched the surface of the CC, thereby increasing its surface area of the activated CC (FCC). Moreover, functional groups such as oxygen-containing groups and N-doping have been introduced. The quantity and type of functional groups introduced during the activation process were related to the anions in the solution. FCC electrodes were used to construct electrochemical sensors for the simultaneous determination of ascorbic acid (AA), dopamine (DA), and uric acid (UA). The PFCC electrode activated by advanced cyclic voltammetry in (NH 4 ) 2 HPO 4 showed linear ranges for AA, DA, and UA concentrations of 0.1 to 2.1 mM, 0.5 to 11 µM, and 0.5 to 11 µM, respectively. The detection limits are 72.93, 0.22, and 0.42 µM, respectively. The good flexibility the PFCC electrode made it suitable for the preparation of flexible sensors. The simultaneous determination of AA, DA, and UA in fetal bovine serum showed a reliable recovery ratio. This study provides a simple and green approach for activating carbon cloth and constructing flexible electrochemical sensors with the potential to simultaneously detect AA, DA, and UA.
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ACS Applied Nano Materials
·
2024
·
IF 5.3
Tingting Li,Yuzhuo Tuo,Yunqi Hao,Jingyu Pang,Miaomiao Tian,Samuel Lalthazuala Rokhum,Fang Zhang,Fang Chai
Determination of Hg2+ is crucial as its high toxicity poses a potential serious threat to food and the environment. Therefore, it is urgent to explore a portable, cost-effective, and visual approach to monitor Hg2+. Herein, ratiometric-constructed dual-emissive carbon dots (P-R-CDs) with dual emissions at 440 and 575 nm were fabricated by blue CDs and orange CDs for visual quantitative detection of Hg2+. The vivid colorimetric sensing can be achieved by quantitative quenching blue emission with Hg2+ and relative stable orange emission. Consecutive fluorescent color transformation with elimination of blue emission by Hg2+ embodied visible distinguishable determination of Hg2+, with a detection limit of 2.9 nM. Furthermore, a smartphone-supported visual sensing platform was established for the determination of Hg2+ by capturing and identifying the RGB value of the variation precisely. The smartphone assistant detection greatly promoted the effectiveness of detection and provided promising on-site application in real samples. The recoveries in real water and food samples revealed the excellent environmental tolerance of P-R-CDs, which represented a prospective application utility for quantitative monitoring of Hg2+ in food and the environment.
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