BMC BIOTECHNOLOGY
·
2026
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IF 4.8
Wang Yingying,Cao Meihui,Hu Mei,Xu Hengrui,Du Yafei,Sun Changxing,Kong Lingfei,Luo Yu,Liu Xiaoyu,Yang Jianming,Liang Bo
Background 3-Hydroxypropionic acid (3-HP) is a promising C3 platform chemical with wide industrial applications. However, its microbial production remains limited by insufficient intracellular malonyl-CoA availability and metabolic imbalance. Result In this study, we systematically engineered Escherichia coli for enhanced 3-HP biosynthesis. The malonate assimilation genes ( matB , smatPQM ) and 3-HP biosynthesis gene ( mcr ) were chromosomally integrated using CRISPR/Cas9, resulting in a plasmid-free, antibiotic-free strain (WYY04) that produced 21.97 mM 3-HP, 0.51-fold higher than the plasmid-based system. Further improvement was achieved by CRISPRi-mediated repression of fatty acid biosynthesis genes ( fabD , fabF ), increasing 3-HP titer by 66%. Introduction of a malonyl-CoA-responsive FapR/fapO biosensor enabled dynamic regulation of mcr expression, enhancing 3-HP production by 59%. Through all these above engineering, the 3-HP production of the strain WYY19 increased by 2.29 times compared to that of the plasmid-expressing system. Under optimized fermentation conditions, the final engineered strain WYY19 produced 42.22 g/L 3-HP with the specific productivity of 0.69 g/g and 0.46 g/L/h from glucose and malonate in fed-batch bioreactor. Conclusions This study demonstrates a robust, genetically stable, and scalable microbial platform for 3-HP biosynthesis.
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Cell Death & Disease
·
2026
·
IF 12.2
Zhang Zhe,Xiao Bing,Jiang Yupeng,Niu Lixia,Wang Li,Cai Juan
Lung adenocarcinoma (LUAD) remains a leading cause of cancer-related mortality worldwide, and understanding its molecular drivers is critical for improving therapeutic outcomes. SUMOylation is a form of post-translational modification that conjugates small ubiquitin-like modifier (SUMO) to specific lysine residues of target proteins. The sole SUMO E2-conjugating enzyme UBC9 is upregulated in multiple malignancies, yet its functional role and specific substrate in LUAD remain poorly defined. Here, we demonstrate that UBC9 is significantly elevated in LUAD tissues, and its high expression is associated with adverse clinic outcomes. Functional assays revealed that genetic ablation of UBC9 robustly suppresses LUAD cell proliferation, migration, invasion, and tumorigenesis both in vitro and in vivo. Through immunoprecipitation-mass spectrometry, we identified Coronin-1C (CORO1C), a master regulator of actin dynamics, as a key substrate of UBC9-mediated SUMOylation. Mechanistically, SUMOylation of CORO1C at lysine residues K19, K311, and K440 enhances its binding to actin-related protein 2 (Arp2) complex, promotes actin‑based cytoskeletal remodeling, and drives malignant cellular behaviors. Collectively, our work reveals a previously unrecognized regulatory axis in which UBC9‑dependent SUMOylation licenses CORO1C to orchestrate Arp2/3‑mediated cytoskeletal dynamics, thereby advancing LUAD progression. These findings reveal CORO1C as a novel SUMOylation target in lung adenocarcinoma and offer new mechanistic insights into tumor cell motility, but also highlight a promising therapeutic avenue for treating advanced LUAD.
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CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Shengquan Shan,Jihao Ma,Yang Chen,Lun Zhang,Chengyang Wang,Yanrui Yang,Ru Zhou,Mei Lyu,Jun Zhu
An anion-cation synergistic strategy is adopted by TbCl 3 post-treatment in Sb 2 (S,Se) 3 solar cells. • This strategy induces the further growth of Sb 2 (S,Se) 3 with favorable crystal orientation. • TbCl 3 -treatment suppresses deep-level defects and adjusts band alignment of Sb 2 (S,Se) 3 films. • The champion device achieves a PCE of 10.11% with excellent stability. Antimony selenosulfide (Sb 2 (S,Se) 3 ) is a promising quasi-one-dimensional photovoltaic material in the application of solar cell field because of its outstanding optoelectronic properties. It is extremely difficult to achieve the Shockley-Queisser limit due to the presence of various defects and the less favorable (hk0) crystal orientation in Sb 2 (S,Se) 3 films. Here, we report an anion-cation synergistic strategy to enhance the quality of Sb 2 (S,Se) 3 films by terbium chloride post-treated process. Both Tb 3+ and Cl − ions are incorporated into the crystal lattice of Sb 2 (S,Se) 3 after post-treatment, which is confirmed by X-ray photoelectron spectroscopy and X-ray diffraction. This anion-cation synergistic effect promotes the Sb 2 (S,Se) 3 films to have a preferred (hk1) crystal orientation, larger grain size, suppressed deep-level defects and more favorable energy band alignment to facilitate charge carrier transport. Consequently, terbium chloride post-treatment enhances the performance of Sb 2 (S,Se) 3 solar cells, achieving a remarkable of 10.11%. Furthermore, the champion devices exhibit an excellent storage, humidity and thermal stability over 800 h aging test. This work provides a valuable strategy for fabricating high-quality of Sb 2 (S,Se) 3 films and high-efficiency inorganic horizontal and vertical PN heterostructure solar cells.
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IEEE SENSORS JOURNAL
·
2026
·
IF 4.5
Lingling Fan,Cunhong Cen,Yuru Wu,Xiaohua Deng,Yuxuan Shi,Yuxing Huang,Xiaohong Tan,Shidong Li,Guiyin Li
Hepatocellular carcinoma (HCC) remains one of the most lethal malignancies worldwide, underscoring the urgent demand for early diagnostic strategies to improve patient survival rates. Glypican-3 (GPC3), a highly specific biomarker of HCC, has emerged as a promising target for early detection. Herein, we developed an innovative magnetic colorimetric aptasensor using ferrosoferric oxide@carbon quantum dots (Fe3O4@CQDs) nanocomposites. The Fe3O4@CQDs nanocomposites combine the magnetic properties of Fe3O4 with the unique optical characteristics of CQDs, exhibiting intrinsic peroxidase‑like activity. The animo-modified GPC3 aptamer (GPC3Apt) was covalently conjugated onto the Fe3O4@CQDs nanocomposites surface via EDC/NHS chemistry to form the recognition probe (Fe3O4@CQDs-GPC3Apt). Upon specific binding between the aptamer and GPC3, the resulting complexes (Fe3O4@CQDs-GPC3Apt-GPC3) modulate the catalytic oxidation of o-phenylenediamine (OPD) by H2O2, generating a yellow oxOPD product whose absorbance intensity correlated with GPC3 concentration. The proposed colorimetric aptasensor demonstrated a detection limit of 3.16 ng/mL and a linear range of 10–200 ng/mL, with high specificity against interfering proteins. It was successfully validated in human serum samples, yielding recoveries between 99.27% and 109.33%. Owing to its operational simplicity and cost‑effectiveness, this platform offers transformative potential for point-of-care HCC screening. Beyond advancing GPC3 detection, the work provides a flexible design paradigm that can be adapted for assays targeting other disease biomarkers.
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Surfaces and Interfaces
·
2026
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IF 6
Mingjun Wu,Yiqing Ouyang,Mingjian Zhao,Yuyao Wu,Zhengying Wang,Qiang Ru
An artificial interphase of Na 3 MnZr(PO 4 ) 3 (NMZP) is engineered on the zinc metal surface as a fast ion conductor to efficiently modulate zinc flux and stabilize the anode. Fabricated via a sol-gel method, NMZP possesses a NASICON-type open 3D framework, high structural stability, robust corrosion resistance, and exceptional ionic conductivity, providing favorable pathways for Zn 2+ diffusion. The NMZP interphase modulates Zn 2+ flux through ionic confinement effects while promoting desolvation of [Zn(H 2 O) 6 ] 2+ species prior to deposition, thereby suppressing parasitic reactions. This dual mechanism ensures uniform zinc nucleation and deposition beneath the protective layer, effectively inhibiting dendritic growth and enhancing cycling stability. Benefiting from robust protective function of the NMZP layer, the NMZP@Zn symmetric cell achieves stable cycling beyond 650 hours at 3.0 mA cm -2 and 1.0 mAh cm -2 with minimal polarization. This study establishes an engineered interphase strategy for realizing long-lived, dendrite-free zinc-ion batteries.
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Russian Journal of Physical Chemistry A
·
2026
·Vol. 100
·期 7
·1348-1359
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IF 0.7
Ren Chaobin,Yaofei Huang,He Zhu,Zhuo Chen,Junlei Shi,Chengxin Shen,Shun Yan,Jingya Peng,Huanxin Li
The solubility properties of coumarin-3-carboxylic acid (CCA) was studied in 12 kinds of alcohol and ester solvents at p = 100 kPa with the temperature range from 278.15 to 318.15 K. It was found that the solubility of CCA increases with the temperature raises. Among the selected solvents, methyl acetate showed the best dissolving ability for CCA, while 2-propanol showed the worst dissolving ability. The obtained data are neatly correlated with four models, including van’t Hoff, λ h , Wilson, and NRTL models, with λ h equation yielding the most accurate predicted results. The dissolving mechanism of CCA was investigated with intermolecular interaction between CCA and solvents by using the COSMO-RS model, and the results indicating that the dissolving process is affected by the intermolecular interaction, molecular polarity, and size of both solvents and CCA. The dissolution thermodynamics analysis shows that the dissolving process of CCA in the twelve solvents is spontaneous, endothermic, and enthalpy driven.
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CHEMICAL ENGINEERING JOURNAL
·
2025
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IF 13.2
Guang Li,Xiangpeng Yang,Lei Huang,Jiaying Wang,Xiangrui Wu,Benhong Xu,Liang Li,Hongbin Yuan,Yuhuan Liu,Jinwen Wang,Lichuang Cao
Despite its longstanding use in recombinant protein production, the fundamental principles governing signal peptide (SP) functionality in Komagataella phaffii ( K. phaffii ) remain poorly understood. In this study, 64 endogenous SPs were identified from K. phaffii genome and were combined with 52 high-performance exogenous SPs from literature. This SP library was employed to optimize secretion of β-galactosidase Bgal1–3-Y497F, an efficient biocatalyst for producing galactooligosaccharide-enriched and low-lactose dairy products. The characterization identified 35 new SPs, expanding the repertoire of SPs that outperformed the conventional α-mating factor (α-MF) SP by 30 %. The top performers, SP25 and W1, mediated 4.0- and 5.5-fold higher extracellular enzyme activity than α-MF SP, with similar enhancements (2.0–5.5-fold) observed for heterologous proteins β-galactosidase BgaD-D and plastic-degrading enzyme LCC-ICCG. A few key sequence features for efficient secretion were revealed. For N-regions, endogenous SPs preferred +1 charge, whereas exogenous SPs preferred +3 charges. For H-region, both SP types showed bell-curve dependencies (extracellular enzyme activity plotted against hydrophobicity) with a consistent optimal window (hydrophobicity scores = 40–55) that provides a general guideline for SP selection and engineering in this host. Implementing an integrated engineering strategy (W1-based SP engineering, multi-copy strain selection, chaperone co-expression, and cultivation condition optimization), a 17.9-fold production increase was achieved. Scale-up to 5-L bioreactor cultivation yielded 7.0 g/L, the highest reported titer of prokaryotic β-galactosidase in K. phaffii . This work provides both fundamental insights into SP features for efficient protein secretion in K. phaffii and a high-performance industrial strain for dairy processing applications.
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EUROPEAN POLYMER JOURNAL
·
2025
·
IF 6.3
Lebing Lian,Xiaotao Bai,Fengguang Jiang,Yongcheng Zhang,Xuan Feng,Min Liu,Quan Zhou
Phthalonitrile resins are widely recognized for their outstanding heat resistance, dielectric properties, and mechanical properties. However, the excessively high melting temperature and narrow processing window constrain their applications in fields such as thermal protection. This study successfully prepared BPL-P resins with enhanced processing, thermal, and mechanical properties by modifying laboratory-synthesized silicone-containing phthalonitrile (BPL) resins with 3-aminophenylacetylene (APA). The alkyne group in APA can self-polymerize to form polyene and trisubstituted benzene structures, and the amino group in APA encourages the conversion of cyano groups into triazine and phthalocyanine. These modifications confer exceptional thermal and thermo-oxidative stability to the resins, with the T d5 of BPL-P2 resin (containing 20 wt% APA) reaching 563.2°C and 522.2°C, respectively. Additionally, the strong solubility and relatively low viscosity of APA significantly enhance the processing performance of the resin. The softening point of BPL-P2 resin decreases from 161°C to 94°C, and its processing window expands from 32°C to 59°C under a viscosity of 1 Pa·s. Moreover, the mechanical properties of the quartz fiber-reinforced composite QF/BPL-P2 were substantially enhanced, with flexural strength increasing from 673.6 MPa to 731.9 MPa at room temperature, and from 115.1 MPa to 358.4 MPa after heat treatment at 400°C for 16 h. These findings broaden the potential applications of phthalonitrile resins in high-end industrial sectors.
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FOOD CHEMISTRY
·
2026
·
IF 10.4
Zipeng Zheng,Jun Hu,Weijie Zhou,Hongqing Yuan,Jianxia Sun,Yongbin Xu,Weibin Bai
Anthocyanins are a class of water-soluble flavonoid pigments widely distributed in plants, characterized by their flavylium cation core. Glycosylation modifications exert significant effects on the biological activities of anthocyanins with identical aglycones. This study accomplished the first chemical syntheses of cyanidin-3-O-galactoside (C3Gal) and cyanidin-3-O-arabinoside (C3Ara), providing a general glycosylation strategy. The configurations of β-D-galactopyranoside and α-L-arabinopyranoside were determined by 2D NMR. Comparative analysis of cyanidin-3-O-glucoside (C3Glu), C3Gal, and C3Ara revealed that C3Glu was the most stable, whereas C3Ara and C3Gal exhibited superior antioxidant activity. C3Ara also showed the strongest inhibition against α-amylase (IC 50 = 98.16 μM), consistent with molecular docking predictions. These results reveal the key structure-activity: hexoses (glucose, galactose) have higher stability and reduced activity. Pentosyl arabinosides have higher biological activity. This work provides a reliable preparation method and research direction for anthocyanin glycosides.
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RESPIRATORY RESEARCH
·
2026
·
IF 5
Zhang Jia,Liu Jin,Chen Yuqian,Chen Huan,Meng Yihan,Wang Yan,Qiu Yuanjie,Hu Huizhong,Shi Wenhua,Li Manxiang,Li Man-xiang
Background Galactose lectin-3 (Gal-3) has been shown to promote the progression of pulmonary arterial hypertension (PAH), yet the intricate molecular mechanisms are still unclear. Methods Primary cultured rat pulmonary arterial smooth muscle cells (PASMCs) and PAH rats were used in this study. Protein phosphorylation and expression levels were identified using Western blotting, while mRNA level was quantified through qRT-PCR. Hemodynamic measurements, hematoxylin-eosin and immunohistochemistry staining were employed to assess the PAH progression. Results Yes-associated protein 1 (YAP1) mediated Gal-3/toll-like receptor 4 (TLR4)-induced PDZ-binding kinase (PBK) upregulation. Furthermore, polo-like kinase 1 (PLK1)/cyclin-dependent kinase 1 (CDK1) mediated Gal-3/TLR4-induced PBK phosphorylation. Activated PBK further phosphorylated protein regulator of cytokinesis 1 (PRC1), which ultimately led to PASMC proliferation. In monocrotaline-induced PAH rat models, inhibition of Gal-3, TLR4, YAP1, CDK1 or silencing of PLK1, PBK, PRC1 attenuated PAH progression. Conclusion Our study presents novel evidence that Gal-3 promotes PASMC proliferation and pulmonary vascular remodeling by activating PBK/PRC1 through the TLR4/YAP1 and TLR4/PLK1/CDK1 signaling pathways, suggesting that this signaling pathway might be a promising target for the treatment of PAH.
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CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Wenping Zha,Yadong Ruan,Zhaoyin Wen
The application of Li 1.5 Al 0.5 Ge 1.5 (PO 4 ) 3 (LAGP) is hindered by chemical instability with lithium (Li) metal and poor interfacial compatibility with high-voltage cathodes. Herein, an in-situ Janus LAGP is fabricated to solve the above two issues simultaneously. On the cathodic side, a succinonitrile-based interlayer with good oxidation stability is applied to facilitate ion transport at the interface and inside the cathode. On the anodic side, Fluoroethylene carbonate (FEC)-based polymer interlayer is chosen to ameliorate side reactions on LAGP/Li interface and inhibit the dendrite growth. Consequently, the Li symmetric cell with the Janus LAGP possesses a high critical current density of 5.4 mA cm −2 and operates stably for over 500 h at a high current density of 1.0 mA cm −2 . Moreover, the Janus LAGP exhibits excellent ionic conductivity (4.76 × 10 −4 S cm −1 ), wide electrochemical window (0–5.2 V vs. Li + /Li) and stable interfacial compatibility with high-voltage cathodes. The solid-state lithium battery (LiNi 0.8 Co 0.1 Mn 0.1 O 2 /Li) delivers a maximum discharge capacity of 178 mAh g −1 with a retention of 85.7% after 100 cycles at 0.5C. Notably, when the mass loading is increased to 10.0 mg cm −2 , the battery delivers a maximum discharge capacity of 134.1 mAh g −1 (1.52 mAh) at 0.5C, and the capacity remains 76.1% after 100 cycles.
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Solar RRL
·
2020
·Vol. 4
·期 9
·2000220
·
IF 7.527
Kanghua Li,Yue Lu,Xiaoxing Ke,Sen Li,Shuaicheng Lu,Chong Wang,Siyu Wang,Chao Chen,Jiang Tang
Antimony chalcogenides (including Sb 2 S 3 , Sb 2 Se 3 , and Sb 2 (S,Se) 3 alloy) have emerged as promising solar absorber materials. Notably, the Sb 2 (S,Se) 3 alloy possesses continuously tunable bandgap from 1.1 to 1.7 eV, which covers the ideal bandgap for single-junction photovoltaics governed by the Shockley–Queisser theory. Moreover, the bandgap gradient provides effective ways for photogenerated carriers collection and has the potential for high-efficient Sb 2 (S,Se) 3 alloy solar cells. Herein, a V-shaped distributional bandgap in Sb 2 (S,Se) 3 solar cells is reported through a simple dual-source vapor transport deposition process, enabling the synergetic increase of the open-circuit voltage ( V OC ) and short-circuit current ( J SC ). Through careful optimization, a power conversion efficiency of 7.27% under AM1.5G illumination is obtained, with V OC and J SC of 0.46 V and 29.6 mA cm −2 , respectively. This V-shaped bandgap engineering provides an effective method to enhance the device performance and can be extended to other chalcogenide thin-film solar cells such as Sn–X, Ge–X, Cu–Sb–X (X = S and Se), and so on.
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危险品化学品经营许可证（带存储）