APPLIED SURFACE SCIENCE
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2025
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IF 6.9
Zong Li,Kaili Ni,Yifei Wang,Yuan Rui,Miaohui Wang,Haiping Lin,Peipei Huang,Qing Li
Achieving high activity and selectivity for multi-carbon (C 2+ ) products on copper-based catalysts at ampere-level current densities is important for advancing industrial electrochemical CO 2 reduction reaction (eCO 2 RR). However, oxidative copper species are prone to reduction to metallic copper at high reduction potentials, causing the decline of selectivity and stability for C 2+ products. Herein, we report that Cucurbit[6]uril (CB[6])-modified copper nanoparticles promote eCO 2 RR via stabilizing Cu + sites at ampere-level current densities. The Cu-CB[6]-3 catalyst exhibited the highest C 2+ productivity with a faradaic efficiency of 72.2 ± 1.0 % at a current density of 1.0 A cm −2 , and the corresponding potential was −1.2 V vs. RHE. Detailed systematical spectroscopic analyses revealed that the main reason for boosting the C–C coupling is that the strong coordination interaction and molecular-level confinement of CO 2 and reaction intermediates of CB[6] can not only stabilize Cu + at higher current densities, but also accelerate the reaction kinetics. Simultaneously, the hydrophobic inner cavity and hydrophilic outer surface of CB[6] molecules ensure the smooth progress of eCO 2 RR while suppressing the hydrogen evolution reaction, resulting in high C 2+ selectivity and long-term stability. This work provides an approach to design highly efficient catalysts for industrial eCO 2 RR.
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BIOPHYSICAL CHEMISTRY
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2025
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IF 2.2
Jing Zhang,Qiang Ma,Honghui Wang,Bin Chen,Yan Li,Yingmin Liao
The rubber antioxidant, N -(1,3-dimethylbutyl)- N ′-phenyl- p -phenylenediamine (6-PPD), as an emerging pollutant, is receiving more and more attention recently. This study investigated the intermolecular interactions of 6-PPD with two key biological macromolecules, human serum albumin (HSA) and alpha-glucosidase (AG), to understand the transport and toxic effects of 6-PPD. Using multiple spectroscopic methods and molecular docking technology, the results demonstrated that 6-PPD could bind to both HSA and AG, thereby inducing fluorescence quenching and conformational changes in both proteins. The binding constants were determined to be (5.93 ± 0.20) × 10 5 and (3.17 ± 0.15) × 10 4 L mol −1 respectively for HSA-6-PPD and AG-6-PPD systems at 298 K, revealing strong binding affinities. Molecular docking identified specific binding sites and non-covalent interactions of the two systems. MD and Energy decomposition analysis revealed the dynamics conformational changes of the complexes and identified van der Waals and electrostatic interactions as primary binding drivers for both systems, while polar solvation energy impeded complex formation. TYR161, ILE142, and TYR138 dominated HSA-6-PPD stabilization, whereas AG-6-PPD was driven by hydrophobic interactions with TRP1369 and VAL1373, with ARG1377 incurring substantial desolvation penalties. Synchronous fluorescence and circular dichroism spectroscopy indicated that 6-PPD binding did not disrupt the microenvironment of Tyr and Trp residues in HSA and AG, while induced structural alterations in HSA and AG that could affect their physiological function. In-vitro tests showed that 6-PPD inhibited AG activity in a dose-dependent manner, with an IC 50 of 8.22 ± 0.44 μmol L −1 . ADMET and PASS online tools was used to predict physicochemical properties and multiorgan toxicity. This work provided insights into the transport and molecular toxicity of 6-PPD, highlighting the adverse biological effects associated with this common rubber additive.
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TALANTA
·
2025
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IF 6.1
Xinyu Zhang,Xuan Zhou,Quan Wan,Taolei Sun,Guanbin Gao
Prussian blue analogue nanozymes (PBANZs) have been widely employed in the colorimetric detection of various organic small molecules due to their peroxidase-like activity. However, the practical applications of PBANZ are limited by its narrow operating pH range, insufficient catalytic activity, and poor stability. In this study, we synthesized a novel Cu 2 Fe(CN) 6 @PVP PBA nanozyme (Cu-PBANZ) using polyvinylpyrrolidone (PVP) as a capping ligand. This novel Cu-PBANZ not only exhibited enhanced peroxidase-like activity and improved stability under near-neutral conditions but also demonstrated higher sensitivity and a broader linear range in the detection of hydrogen peroxide (20–4000 μM, LOD 5 μM) and ascorbic acid (10–2000 μM, LOD 3.9 μM). Besides, a smartphone-based platform was developed for detecting AA content in fruits, achieving a detection limit of 7.8 μM. Both UV–Vis spectrophotometry and smartphone-based colorimetry were validated with recovery rates of 95.1 %–106.7 % and 94.8 %–108.9 %, respectively. This study proposed a promising biosensor incorporating Cu-PBANZ for rapid colorimetric detection of AA in fruits using a smartphone.
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ACS Omega
·
2025
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IF 4.3
Songzhi Li,Yang Guo,Hong Jiang,Huan Zhang,Jiayu Li,Yanli Chen,Jie Li,Xiangzhao Mao,Minxiao Wang
Arylsulfatase catalyzes the cleavage of sulfate ester bonds and plays a role in agar desulfation, thereby enhancing agar gel strength and quality. While studying the desulfurization pathway in Pseudoalteromonassp. SR43-6, a sequence encoding a potential arylsulfatase─Pseudoalteromonas Ars (Ps-Ars)─was found. The enzyme, with p-nitrophenyl sulfate as a substrate, exhibited optimal activity at 35 °C and pH 8.0. Its relative activity (206 U/mg) exceeded that of the recently identified arylsulfatases. Four hundred units of the enzyme removed 86.4% of sulfate groups from Gelidium amansii agar in 4 h, whereas 800 U of the enzyme removed 71.3% of sulfate groups from Gracilaria lemaneiformis agar in 8 h. After enzymatic treatment, G. amansii agar gel strength was enhanced by 32%, and a similar improvement was observed in G. lemaneiformis agar gel strength. Enzymatic agar desulfurization offers mild, quality-retaining, and environmentally friendly advantages, augmenting industrial application prospects.
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ENVIRONMENTAL POLLUTION
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2025
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IF 7.3
Qian Wang,Tao Zhang,Xuemei Liu,Shuo Liu,Xinyue Wang,Xiaoming Wang
Although 6:2 fluorotelomer alcohol (6:2 FTOH) is a common compound in landfill leachate, its anaerobic biotransformation and partitioning remain poorly understood. This study investigated the anaerobic biotransformation and partitioning of 6:2 FTOH in landfill leachate microcosms. At the end of the experiment, 19.4 mol% of the initial 6:2 FTOH partitioned into the gas phase. Therefore, anaerobic leachate could represent a significant pathway for semi-volatile 6:2 FTOH to the landfill gas or enter the atmosphere. The anaerobic biotransformation of 6:2 FTOH in leachate conformed to the first-order bi-exponential degradation model and the half-life was 12 days. The 6:2 fluorotelomer carboxylic acid (6:2 FTCA) was the main biotransformation product, accounting for 8 mol%. Moreover, microbial community composition showed the genus of Pseudomonas, DMER64, and Fastidiosipila may play a role in the biotransformation of 6:2 FTOH. This study elucidates the potential significance of biotransformation processes on both the partitioning and environmental fate of 6:2 FTOH within landfill leachate.
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JOURNAL OF MOLECULAR STRUCTURE
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2025
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IF 4.7
Dantong Wang,Hanqing Xie,Yongqi Zeng,Fengxi Li,Lei Wang,Peng Chen,Zhi Wang
This study presented, for the first time, a method for synthesizing 4-amino-3,5-dicyano-6-aryl phthalates using porcine pancreatic lipase (PPL) as a catalyst. We synthesized 18 derivatives of 4-amino-3,5-dicyano-6-aryl phthalates employing aryl aldehyde, acetylenic esters, and malononitrile under optimal conditions for 4 h, with water serving as the primary reaction medium. The yields of the target products ranged from moderate to high, achieving 43 % to 95 %. This method demonstrates an excellent tolerance for various functional groups. Furthermore, this paper elucidates the reaction mechanism through molecular docking studies and confirms the interaction between the substrate and lipase. The data indicate that the measured distance from the substrate to the catalytic triad is 3.8 Å, with a binding energy of -4.6 kJ/mol, suggesting that PPL demonstrates the strongest catalytic potential in this specific reaction. Additionally, the aforementioned products underwent molecular docking analysis with three selected enzyme targets from Leishmania, highlighting their potential as lead compounds for anti-Leishmania activity.
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Materials
·
2025
·Vol. 18
·期 10
·2289
·
IF 3.2
Junyao Shen,Tian Si,Huan Gao,Linhua Zhu,Heng Zhang,Xin Gao,Xiaoning Tang
ZrO 2 and multi-walled carbon nanotubes (MWCNTs) were selected as single-phase and composite toughening agents to investigate the influence on the mechanical properties of CaZr 4 (PO 4 ) 6 (CZP) ceramics. The results revealed that the addition of single-phase or composite toughening agents had minimal impact on the phase composition and crystallinity of CZP ceramics. When the content of the single-phase ZrO 2 toughening agent reached 10 wt.%, the flexural strength of CZP ceramics increased to 71.60 MPa due to the particle toughening mechanism of ZrO 2 . With the addition of 1.0 wt.% ZrO 2 and 0.3 wt.% MWCNTs, the CZP ceramics demonstrated enhanced densification and improved sintering activity. The small-sized ZrO 2 particles were evenly dispersed within the ceramic matrix, accompanied by a phase transformation during sintering. Together with MWCNTs, this combination resulted in a significant increase in flexural strength, reaching 138.43 MPa. An in-depth analysis of the toughening mechanisms indicated that the CZP ceramic matrix primarily featured ZrO 2 phase transformation toughening and the pull-out and bridging toughening provided by MWCNTs. The synergistic interaction of these multiple toughening mechanisms significantly enhanced the mechanical properties of CZP ceramics, providing valuable theoretical insights for optimizing the performance of phosphate ceramics in practical applications.
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LANGMUIR
·
2025
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IF 3.9
Heng Li,Chengzhong Zhang,Yuhang Jiang,Wenwu Ni,Mingshuo Li,Jing Wang
Widespread dye contamination poses a serious threat to humans and aquatic environments. Metal–organic frameworks (MOFs) with abundant reactive groups and high specific surfaces are candidates for the adsorption of dyes. In this study, good growth of UiO-66-SO3H crystals on the surface of nylon-6 fibers was achieved based on the idea of in situ growth. UiO-66-SO3H with a sulfonic acid group was successfully introduced into nylon-6 electrostatic spinning fiber, and the UiO-66-SO3H@nylon-6 membrane with high specific surface area and a three-dimensional mesh structure was obtained, which effectively solved the problems of clogging of the equipment, difficulty in being separated and recovered from the water, the possible secondary pollution of the water environment caused by the powder form of the MOF material, etc. The UiO-66-SO3H@nylon-6 membrane has a good adsorption capacity for rhodamine B (RhB), and the experimental data are consistent with the pseudo-second-order kinetic model and Langmuir model, which shows that the adsorption is a spontaneous heat absorption process. Pore-filling, hydrogen bonding, π–π interactions, and electrostatic attraction induced by –SO3H are the adsorption mechanisms of RhB on this adsorbent. In conclusion, the UiO-66-SO3H@nylon-6 membrane is an adsorbent capable of rapid adsorption of RhB and can be easily separated from water for recovery, regeneration, and utilization. This study may provide ideas for the application of MOFs in the field of pollutant removal.
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MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 7
·1-11
·
IF 5.3
Zhuang Pujian,Chen Sisi,Chen Ruichao,Chen Zhiwei,Han Luodan,Zhang Xi,Yao Xu,Wu Fang,Shi Dongmei,Chen Jinghua,Lan Jianming
An upconversion luminescence (UCL) nanosensor was developed based on the inner filter effect (IFE) mechanism. This involved the interaction between polyacrylic acid (PAA)-coated NaYF 4 :Yb/Er upconversion nanoparticles (UCNPs) and the oxidation product (oxDPD) of N , N -diethyl- p -phenylenediamine (DPD). DPD was oxidized to oxDPD through the combined action of H 2 O 2 and horseradish peroxidase (HRP). As a result, there was a large overlap between the UCL spectrum (545 nm) of Er-doped UCNPs and the UV–Vis absorption spectrum (552 nm) of oxDPD, which subsequently caused the quenching of UCL via the IFE. When 6-mercaptopurine (6-MP) reacted with oxDPD, the absorption intensity of the resulting product at 552 nm decreased, thereby restoring the intensity of UCL. Therefore, 6-MP can be sensitively detected according to the intensity change of UCL. The resulting detection range and the limit of detection (LOD) were 0–15 μg·mL −1 and 0.56 μg·mL −1 , respectively. The “off–on” UCL nanosensor designed for the detection of 6-MP exhibits the advantages of easy operation, rapid response time, and high sensitivity, demonstrating promising potential for application in clinical monitoring. Graphical
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Heliyon
·
2023
·
IF 4
Liang Hao,Yaxin Qi,Yingchun Wu,Donghui Xia
A simple solid phase extraction (SPE) coupled with spectrophotometric method was developed for determination of trace permanganate (MnO 4 − ) in water. Commercial reagent polyamined-6 powder was used as SPE stationary phase to retain MnO 4 − which is based on the fact that MnO 4 − can be adsorbed on polyamide 6 (PA-6) sorbent as water samples flow through the SPE cartridge. 3,3′,5,5′-tetramethylbenzidine (TMB) were used both as eluent solution and chromogenic agent to convert the adsorbed MnO 4 − into Mn 2+ and generate blue oxidized product with high molar absorptivity. Quantification of MnO 4 − could be obtained by spectrophotometric detection of the resulting solution flow out of the SPE cartridge. The extraction and detection variables was optimized to maximize the absorbance of the TMB oxidized product. Under optimized conditions, this method provided linear dynamic ranges of 0.01–1 μmol L −1 for MnO 4 − with preconcentration of 100 mL water sample. The limits of detection (3S b /m) of this method in deionized water were calculated to be 5.1 nM, and the relative standard deviations were determined to be 3.2% (C = 0.1 μmol L −1 , n = 5). This method has been applied to the determination of MnO 4 − in spiked tap waters and satisfactory recovery was obtained.
研究领域:
分析化学
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环境科学
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Nanoscale Advances
·
2023
·Vol. 5
·期 20
·5661-5670
·
IF 4.7
Tingyu Xue,Fang Liu,Bin Lu,Qingrong Dong,Bin Zhao,Tianqing Chen,Kun Zhang,Jianguo Li,Jiangfeng Du
Radioactive cesium (Cs) is a significant concern due to its role as a major byproduct of nuclear fission and its potential for radioactive contamination. Internal contamination with radioactive Cs is characterized by immoderate production of reactive oxygen species (ROS), resulting in severe radiation damage. Therefore, the development of therapeutic strategies should focus on enhancing the excretion of radioactive Cs and reducing radiation-induced oxidative damage. However, current therapeutic drugs like Prussian blue (PB) have limited efficacy in addressing these issues. In this study, we present Cu3[Fe(CN)6]2 (CuFe) nanoparticles, a Prussian blue analog (PBA), which can not only efficiently sequester Cs but also exhibit resistance against radiation damage. The results of the adsorption studies demonstrate that CuFe outperforms PB in terms of adsorption performance. Further mechanistic investigations indicate that the increased adsorption capacity of CuFe may be attributed to the presence of additional defects resulting from the [Fe(CN)6] missing linkers. Moreover, CuFe mimics the functions of catalase (CAT) and superoxide dismutase (SOD) by effectively eliminating O2˙− and H2O2 while scavenging ˙OH, thereby mitigating ROS induced by radiative Cs. Importantly, in vivo study confirms the efficient Cs decorporation capability of CuFe. The fecal cumulative excretion rate of CuFe reaches 69.5%, which is 1.45 times higher than that of PB (48.8%). These findings demonstrate that CuFe exhibits excellent Cs removal performance and ROS scavenging ability, making it an attractive candidate for the treatment of Cs contamination.
研究领域:
药理学
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放射医学
·
纳米医学
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BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
·
2022
·
IF 3.322
Wei Sun,Jing Liu,Renhao Zhao,Teng Yang,Zhichen Zheng,Tongyu Zhang,Guofeng Wang
Background and Purpose: To investigate the biological role of interferon α/β receptor 2 (IFNAR2) in type 1 diabetes (T1D). Methods First, IFNAR2 mRNA and protein expression levels in serum of T1D patients and healthy controls were detected by RT-qPCR and Western blot . For experimental studies, 80 male C57BL/6 mice were randomly divided into 4 groups with 20 mice in each group: the control group, the T1D group, the T1D + ad-con group and the T1D + ad-si-IFNAR2 group. The T1D mouse model was generated by multiple intraperitoneal injections of small doses of streptozotocin (STZ). Body weight and blood glucose levels were measured weekly until 6 weeks. After 6 weeks, all mice were sacrificed and the levels of insulin (Ins), tumor necrosis factor α (TNF-α), interleukin 4 (IL-4), IL-6, and type I interferon γ (IFN-γ), IFNAR2 protein expression, the number of dendritic cells (DCs), and changes in islet β cells were assessed. Results IFNAR2 mRNA and protein expression levels in serum of T1D patients were significantly higher than those in healthy controls ( P < 0.05). Furthermore, IFNAR2 protein expression, number of DCs, and IFNAR2 mRNA, blood glucose, TNF-α, and IFN-γ levels were significantly upregulated in T1D mice compared with the control group ( P < 0.05), while weight, and Ins, IL-6, and IL-4 levels were decreased ( P < 0.05). However, knockdown of IFNAR2 reversed these trends. There was no significant difference in markers between the T1D + ad-con group and the T1D group ( P > 0.05). Conclusions Knockdown of IFNAR2 reduced the inflammatory response and improved islet function of T1D mice.
研究领域:
免疫学
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糖尿病
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分子生物学
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