Food Bioscience
·
2024
·
IF 4.8
Jinyue Sun,Han-Xiao Zhuang,Jia-Neng Pan,Qian-Jun Shen,Hui-Ling Shen,Wen-Wen Zhou
This study has investigated cuproptosis caused by copper sulfate (CuSO 4 ) in Escherichia coli O157: H7 and its mechanism. Meanwhile, the mechanism of CuSO 4 combined with natamycin (Nata) against E. coli O157: H7 planktonic and biofilm cells were also investigated. Finally, we prepared an agar-based film, researched its properties, and used it for packaging cherry tomatoes and purple kale. The results indicated that CuSO 4 could cause cuproptosis in E. coli O157: H7 and lead the genes related to Fe-S cluster protein ( hyfA , sufB , xdhC , and narY ) to down-regulate and cuproptosis-related proteins to change. Then, CuSO 4 combined with Nata could synergistically decrease E. coli O157: H7 bacterial counts in planktonic condition and biofilm by synergistically affecting the membrane potential, cytoplasm leakage, permeability of outer membrane and inner membrane. After that, CuSO 4 and Nata could change the mechanical properties, color and optical characteristics of agar-based film, and decrease the water vapor permeability and oxygen permeability value. Finally, CuSO 4 @Nata@Carboxymethylcellulose@Agar film effectively lowered the counts of E. coli O157: H7 in cherry tomatoes and purple kale during the storage.
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Foods
·
2024
·Vol. 13
·期 20
·3295
·
IF 4.7
Kok Bing Tan,Meixia Zheng,Junyan Lin,Yujing Zhu,Guowu Zhan,Jianfu Chen
Loquat leaf extract (LLE) was added to guar gum and pullulan as an environmentally friendly packaging film (GPE) to preserve Chinese water chestnuts (CWCs). The effect of the amount of LLE on the guar gum/pullulan composite film was investigated. The optimal amount of LLE was 4% (GPE4), with lower water vapor permeability (WVP) and greater mechanical strength, antioxidant, and comparable antibacterial performance than many pullulan-based films. Upon packing the CWCs for 4 days, the weight loss rate of GPE4 was only 1.80 ± 0.05%. For GPE4, the POD activity, the soluble solid content, and the vitamin C (Vc) content of the CWCs were 21.61%, 36.16%, and 26.22% higher than those of the control sample, respectively. More importantly, GPE4 was effective in preserving the quality of CWCs after 4 days of storage, better or at least comparable to non-biodegradable plastic wrapping (PE). Therefore, it can be concluded that GPE films hold significant promise as a sustainable alternative packaging material for preserving fruit-based foods like CWCs, potentially replacing PE in the future.
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STARCH-STARKE
·
2025
·
IF 2.7
Ge Yan,Qifeng Chen
In this study, polyphenols extracted from apple waste were blended with modified nanocellulose and modified microcrystalline cellulose to produce composite films based on water chestnut starch. Thermogravimetric analysis indicated that the addition of polyphenols from apple residue could enhance the heat endurance of the water chestnut starch film. The study findings suggested that the incorporation of M-NCC led to a 24.35% boost in the tensile strength of the finished films, while that with M-MCC increased by 55.17%. The hydrophobic properties were improved due to the introduction of non-polar carbon chains and hydrophobic ester groups, resulting in respective increases in the surface contact angle of 22.66% and 24.94%. The incorporation of polyphenols from apple residue led to a 16.05% increase in the water solubility of the starch film, and the incorporation of M-NCC and M-MCC resulted in a decrease in the water solubility of the composite films by 19.04% and 30.79%, respectively. Moreover, the DPPH free radical scavenging rates of the films containing apple polyphenols (P, PN, and PM) were increased by 164.7%, 163.3%, and 134.7%, demonstrating excellent antioxidant properties. These findings suggested that the combination of apple pomace polyphenols and cellulose nanocrystals offered an optional approach for preparing food packaging films.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Chao Zhang,Chunhe Gu,Mengrui Wang,Junxia Chen,Haode Chang,Ziqing Chang,Junping Zhou,Mingzhe Yue,Wenjing Zhang,Quanmiao Zhang,Zhen Feng
Noni fruit has an unpleasant flavour but is highly bioactive. Therefore, it is necessary to clarify the effect of temperature regulation on quality of fermented noni fruit. In the present study, the formation of flavours, amino acid profiles, and iridoid glycosides during noni fruit fermentation at different temperatures were investigated. We initially found that different temperatures affected core microbial communities. The general evolutionary trends of Acetobacter and Gluconobacter were influenced by different temperatures. Furthermore, high temperature helped maintain low octanoic and hexanoic acids. Subsequently, we found that high temperature improved total amino acids and iridoid glycosides. The correlation network analysis revealed that bacterial communities impacted the quality (volatile flavours, amino acid profiles, and iridoid glycosides) of fermented noni fruit. Overall, altering the temperature induced variations in microbial communities and quality during the noni fruit fermentation process. These results are instrumental in the pursuit of quality control in natural fermentation processes.
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JOURNAL OF CHROMATOGRAPHY A
·
2019
·
IF 3.858
Yinghe Ji,Zhaorui Meng,Jing Zhao,Hanqing Zhao,Longshan Zhao
A sensitive, fast, eco-friendly ultrasound assisted liquid–liquid microextraction (LLME) in combination with hydrophobic deep eutectic solvent (DES) method was developed to preconcentrate and extract sulfonamides in fruit juices, prior to high-performance liquid chromatographic (HPLC) analysis. The DES was synthesized with trioctylmethylammonium chloride (TAC) and 2-octanol at the molar ratio of 1:2. Some parameters which affected the extraction efficiency were investigated and optimized, including the volume of DES, extraction method, extraction time, pH. Under the optimum conditions, good linearity ( r = 0.9999) in the range of 0.1–50 µg mL −1 was obtained. The limit of detection (LOD) was 0.02–0.05 µg mL −1 . And the accuracy of the developed method was confirmed by analysis of spike method, the recoveries were in the range of 88.09–97.84% at the spike levels of 2–20 µg mL −1 in fruit juices.
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Food Bioscience
·
2024
·
IF 4.8
Fuhao Tang,Baoyao Wei,Chao Qin,Li Huang,Ning Xia,Jianwen Teng
Passion fruit peel contained various phenolic compounds with α-glucosidase and α-amylase inhibitory activities in vitro . However, the bound state and glycosylated forms hindered their active expression. In this article, β-glucosidase-producing Lactobacilli were inoculated into fermented peels for targeted metabolism. The results showed that the release of bound polyphenols and the increase of free polyphenols were significantly correlated with β-glucosidase activity. The strain G1 with high enzyme activity degraded 48.20% of the bound polyphenols after 24 h. The ability of extracts to inhibit α-glucosidase and α-amylase activities was significantly enhanced and closely related to the content of free polyphenols (r = -0.96 and -0.88, P < 0.001). Computer- assisted screening identified 38 phenolic compounds as potential dual inhibitors of α-glucosidase and α-amylase. Among them, 22 α-glucosidase inhibitors and 24 α-amylase inhibitors were identified for the first time. Various inhibitors, including phenolic acids, flavones, and flavonols, increased in content after fermentation. The total content of 14 glycosylated polyphenols decreased to 28.38% at 48 h, which was opposite to the aglycone. Molecular docking indicated that glycosylated polyphenols bind to β-glucosidase through hydrogen bonds and van der Waals forces. Different glycosylations at the C-3 position of quercetin bound at same sites to β-glucosidase. This study contributes to the theory of enhancing the functional activity of natural products through targeted fermentation transformation.
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JOURNAL OF SEPARATION SCIENCE
·
2025
·Vol. 48
·期 3
·e70118
·
IF 2.8
Yu Jiang,Hong Pan,Menghong Xie,Huifeng Deng,Guihua Ruan,Yipeng Huang
In this study, magnetic Fe 3 O 4 -coated metal-organic framework composites, CONH 2 -MIL-101(Cr)/Fe 3 O 4 , were synthesized by depositing Fe 3 O 4 particles on the surface of amidated MIL-101(Cr). The scanning electron microscope images show that the MIL-101(Cr) crystals were uniformly coated by Fe 3 O 4 particles. The magnetic hysteresis curve of CONH 2 -MIL-101(Cr)/Fe 3 O 4 reveals a saturated magnetization value of 23.47 emu/g. The water contact angle decreases from 61.00° to 12.88° after amidation and Fe 3 O 4 coating. The magnetic solid-phase extraction (MSPE) efficiency of CONH 2 -MIL-101(Cr)/Fe 3 O 4 for benzoic acid derivatives was studied. Under optimal conditions, the adsorption and desorption processes can be completed within 5 min, and the maximum adsorption capacity to protocatechuic acid (PCA) and vanillic acid (VA) is 10.31 and 17.30 mg/g. Coupling the MSPE with high-performance liquid chromatography-ultraviolet detection, the detection limit for PCA and VA was 0.29 and 0.58 ng/mL, respectively. The mean recoveries of PCA and VA in spiked samples ranged from 82.85%–91.27% with relative standard deviations lower than 0.33%–5.33%. This study provides inspiration for the fabrication of novel magnetic sorbents, and a reliable method for the adsorption and analysis of benzoic acid derivatives.
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POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2025
·
IF 6.8
Hua Huang,Genlin Mao,Dongjie Liu,Shenghua Ding
The cuticle layer covering on surface plays important roles in the quality changes including visual of appearance, texture, and water loss for fruit. The surface microstructures, chemical composition of epi- and intracuticular waxes, and cutin monomers, as well as their effects on fruit transpiration in Sanhua plum were comprehensively carried out. Microscopic observation revealed an irregular surface morphology characterized by continuous crusts interspersed with sparsely distributed nano-granular crystals. Chemical analysis demonstrated that the epicuticular waxes were primarily composed of very-long-chain n -alkanes, secondary alcohols, aldehydes, fatty acids, primary alcohols, and alkyl esters. The intracuticular waxes contained comparable levels of very-long-chain components and pentacyclic triterpenoids. The cutin monomers were predominantly C 16 -dihydroxy fatty acids, and other hydroxyl fatty acid derivatives, present with a moderate level as other reported Rosaceae family fruit. Compared to cutin polymers, the cuticular waxes contributed approximately 76.9 % of the total barrier to water loss, with the epicuticular wax layer exhibiting 1.6-fold to the intracuticular wax layer for the barrier efficiency in Sanhua plum fruit. The elucidation of the potential effect of surface lipids on transpiration was likely associated with the structural and chemical distribution of cuticular wax layers within the cuticle.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Rui Cheng,Yiqun Wan,Xuejin Mao,Hao Wan,Fan Liu,Lan Guo,Bingzhen Zhang
This work presents an efficient sorbent for plant growth regulators (PGRs) by regulating the defects of a metal–organic framework MIL-101(Cr). Using the regulated MIL-101(Cr), we developed a simple and effective method for the simultaneous determination of eleven PGRs in fresh fruit juice. The extraction conditions were optimized by an orthogonal array design. Under optimal conditions, the method showed a satisfactory limit of detection (0.1–1.2 ng/g), recovery rates (83.4–110.2 %), and precision (2.9–18.0 % for intra-day and 2.7–10.8 % for inter-day), as well as a greatly suppressed matrix effect. Notably, regulating the defects significantly enhanced the desorption of PGRs on MIL-101(Cr). The sorbent didn’t need to be destroyed to release the adsorbed PGRs and could be reused at least 6 times. Furthermore, the defects of MIL-101(Cr) and interactions between the sorbent and PGRs were studied by TGA, ATR-IR, XPS, NH 3 -TPD and UV–Vis DRS.
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POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2025
·
IF 6.8
Qiuli OuYang,MingChen Yang,Yonghua Zhang,Lu Li,Xiaoli Tan,Nengguo Tao
Huge postharvest citrus infections are produced by Penicillium digitatum . The antifungal activity of sodium trans -2-hexenylate (STH) against the P. digitatum growth and possible mechanism were investigated. The minimal inhibitory and fungicidal concentrations of STH against P. digitatum were 0.4 and 0.8 g L –1 , respectively. STH decreased the green mold incidence of Ponkan fruit without negatively impacting fruit quality. STH altered and distorted the external appearance of P. digitatum mycelia rather than the cell wall. Correspondingly, STH decreased the lipid and ergosterol levels while degrading the cell membrane integrity. Meanwhile, STH affected mitochondrial function by lowering the mitochondria membrane potential (MMP) and ATP levels, as well as malate dehydrogenase (MDH) and succinate dehydrogenase (SDH) activities. Furthermore, MMP damage occurred earlier than that of cell membrane damage. Our current study showed that STH may be an acceptable substitute to chemical fungicide in postharvest disease control in citrus fruit and its underlying inhibitory mechanism is attributed to its ability to impair the mitochondrial function of P. digitatum .
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ACS Sensors
·
2025
·
IF 9.1
Xiaopeng She,Mengqing Wang,Xinke Jiang,Yi Chen,Cheng Zou,Hongping Liang,Yong Zhou
Ethylene (C2H4) serves as a gaseous biomarker of fruit ripeness during the stages of growth and storage, thus necessitating swift and sensitive detection to assess the evolution of maturity and flavor for target fruits. However, C2H4 vapor is difficult to discern due to its nonpolar inertness and robust molecular structure at room temperature (RT). To this end, a new chemiresistive sensor based on bismuth selenide (Bi2Se3) decorated with zinc oxide (ZnO) nanorods was prepared in this work. Contrary to the high sensor resistance and lack of signal toward C2H4 under dark environments at RT, UV illumination endowed the composite film sensor with low resistance and a noticeable signal. Furthermore, the recovery speed was intriguingly reduced from 10 min to less than 2 min when water vapor was simultaneously introduced. In particular, the optimal 1.0-Bi2Se3/ZnO sensor delivered a response of 38.7% and a response/recovery time of 27/87 s toward 5 ppm of C2H4. Also, the long-term stability was remarkably improved with respect to the pure ZnO counterpart. In addition, excellent linearity across the concentration range from 1 to 20 ppm, good repeatability, and high selectivity over C2H4 were displayed. The effective on-site freshness detection of bananas via the as-prepared 1.0-Bi2Se3/ZnO sensor, by combining electrical signal and visual information demonstrated a huge application potential for fruit ripening monitoring under the synergistic effect of UV illumination and humidity at RT, well catering to the demanding requirements of low power consumption and high miniaturization in the field of future smart sensors.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Dongsheng Fu,Yuanzheng Ding,Ruijie Guo,Jie Zhang,Huifang Wang,Baolong Niu,Hong Yan
ZIF-8, a subclass of metal organic frameworks (MOFs), was employed as the CuO carriers because of its high surface areas and good dispersibility. A novel antibacterial agent CuO@ZIF-8 was synthesized by environmentally-friendly direct calcination strategy, and introduced into the composite double-layer films for packing materials. The double-layer films were prepared via solution casting method with polylactic acid (PLA) and polyvinyl alcohol (PVA)-quaternary ammonium chitosan as the matrix of outer layer and inner layer, respectively; and CuO@ZIF-8 nanoparticles were introduced into the PVA-quaternary ammonium chitosan layer. The double-layer films exhibited superior antibacterial activity resulted from the uniform dispersion of CuO by ZIF-8 carriers. The elongation at break was enhanced and up to 17.13%, about 2.4-fold that of PLA films. Meanwhile, the films provided low water vapor permeability and strong UV-barrier ability which were attributed to the lay-by-layer casting, CuO@ZIF-8 doping and TiO 2 addition. Cherry tomato preservation experiment revealed that the composite films retarded the growth of harmful microorganisms on the fruit surface. MTT assay confirmed the cytocompatibility of the films. The easily fabricated double-layer films presented potential possibility in the field of biodegradable food packaging.
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