Nanoscale Advances
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2023
·Vol. 5
·期 3
·830-839
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IF 4.7
Yazhou Huang,Yunfei Zhang,Junyan Lv,Yinfeng Shao,Dongfang Yang,Yuan Cong
The sensitive detection of cardiac troponin I (cTnI) is of great significance for the early diagnosis of acute myocardial infarction (AMI). Herein, in order to fabricate an electrochemical biosensor for ultrasensitive cTnI detection, atomic layer deposition (ALD) was employed to directly deposit NbS2 nanoflakes (NFs) on carbon fiber paper (CFP). Due to the self-limiting reaction of ALD, NbS2NFs were deposited uniformly and accurately on the surface of carbon fibers by controlling the number of ALD cycles, which ensured ultrasensitive detection. Precise regulation of the nanoscale morphology and electrochemical performance of NbS2 nanoflakes via ALD cycles was observed in depth. Owing to the high surface area and conductivity, an anodic/cathodic current of ∼3.01 mA of NbS2NFs/CFP can be obtained. Subsequently, an electrochemical biosensor based on the excellent performance of NbS2NFs/CFP was fabricated. The ultrasensitive detection of cTnI in a linear range of 1 fM to 0.1 nM with a detection limit of 0.32 fM was achieved.
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MOLECULES
·
2023
·Vol. 28
·期 14
·5368
·
IF 4.6
Jinghong Shi,Yucheng Wang,Wei He,Ziyue Ye,Mengli Liu,Zheng Zhao,Jacky Wing Yip Lam,Pengfei Zhang,Ryan Tsz Kin Kwok,Ben Zhong Tang
Biofilm-associated infections exert more severe and harmful attacks on human health since they can accelerate the generation and development of the antibiotic resistance of the embedded bacteria. Anti-biofilm materials and techniques that can eliminate biofilms effectively are in urgent demand. Therefore, we designed a type I photosensitizer (TTTDM) with an aggregation-induced emission (AIE) property and used F-127 to encapsulate the TTTDM into nanoparticles (F-127 AIE NPs). The NPs exhibit highly efficient ROS generation by enhancing intramolecular D–A interaction and confining molecular non-radiative transitions. Furthermore, the NPs can sufficiently penetrate the biofilm matrix and then detect and eliminate mature bacterial biofilms upon white light irradiation. This strategy holds great promise for the rapid detection and eradication of bacterial biofilms.
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International Journal of Electrochemical Science
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2023
·
IF 1.5
Ping Wang,Huikai Ma,Yaoyao Xie,Sanqiang Li
In this study, a ratiometric electrochemical aptasensor was proposed for the highly sensitive detection of carcinoembryonic antigen (CEA) with exonuclease I (Exo I)-assisted target recycling amplification. The thiolated methylene blue (MB)-labelled hairpin capture probe (MB-HP) was immobilized on the surface of gold nanoparticles (AuNPs) modified gold electrode (AuE) via Au-S bonding, and the hybridization between MB-HP and ferrocene (Fc)-labelled aptamer (Fc-Apt) formed a duplex structure, leading to Fc molecules approaching to the electrode surface while MB molecules were far away. In the presence of CEA, its specific recognition with the aptamer caused the release of Fc-Apt from the electrode surface and subsequently the formation of a hairpin structure for MB-HP, resulting in both “signal-on” of MB (I MB ) and “signal-off” of Fc (I Fc ). To improve the sensitivity of the aptasensor, an Exo I-assisted target recycling amplification strategy was designed to achieve an amplified ratiometric signal. Under the optimal conditions, the proposed aptasensor showed a linear detection range from 10 pg mL -1 to 100 ng mL -1 with a detection limit of 4.1 pg mL -1 . Furthermore, the stability, reproducibility, selectively and real sample detection ability of the aptasensor were investigated and satisfactory results were obtained.
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Journal of Materials Chemistry C
·
2023
·Vol. 11
·期 38
·13030-13038
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IF 6.4
Jie Chen,Yu Li,Zhe Yin,Shuaibing Wang,Ouyang Lin,Wentao Niu,Feng Teng,Aiwei Tang
Cu-based halide perovskites have attracted extensive attention in the optoelectronic field owing to their non-toxic nature and excellent stability. Nevertheless, the growth mechanism of Cu-based halide perovskite nanocrystals (NCs) remains unclear, and the size and uniformity of NCs are difficult to control, which seriously hinders the development of these materials. Herein, a facile hot-injection method was applied to control the size and uniformity of blue-emitting (450 nm) Cs3Cu2I5 NCs. The dimensions of Cu-based perovskites were tuned from zero-dimensional NCs to one-dimensional nanorods. Additionally, the sizes of Cs3Cu2I5 NCs could be tuned from 22.1 ± 1.5 to 8.9 ± 1.1 nm by varying the reaction temperature and the dosage of cesium oleate, which is also extended to tune the size of Cs3Cu2X5 (X = Br and Cl) NCs. Interestingly, the photoluminescence (PL) maximum at 450 nm was almost unchanged with the size tuning, indicating that PL is from the radiation recombination of self-trap exciton emission. Moreover, the phase transition temperature of Cs3Cu2X5 NCs was regulated by varying the size, where a small size is beneficial to the decrease of the phase transition temperature. As a demonstration, the different-sized Cs3Cu2X5 NCs exhibited a size-dependent temperature response, and the response time became shorter as the size decreased. The temperature response of the small-sized Cs3Cu2X5 NCs could be decreased to only 15 s, which is better than that of their counterparts reported previously. This work may deepen the understanding of the size-dependent phase transition process in Cu-based perovskite NCs.
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JOURNAL OF MOLECULAR STRUCTURE
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2023
·
IF 3.8
Yong-Po Zhang,Qing He,Xin-Hui Zhou,Guang-Huan Liu,Ai-Qin Yue,Chun-Yan Gao,Jin-Zhong Zhao,Wei-Jun Du,Shi-Ping Yan
Two new Cu(І) complexes, [Cu 2 (L 1 ) 2 (μ-Br) 2 ] (1) and [Cu 2 (L 1 ) 2 (μ-Cl) 2 ] (2) were synthesized from Cu(II) halogen salts and the reduced Schiff base ligand. Stable Cu(І) complexes were obtained via in situ reduction of Cu(II) to Cu(I) and the C N was oxidize to C = N in the ligand. The structures of two complexes were characterized by X-ray single crystal diffraction analysis as well as IR and UV–Vis spectra. The two complexes have potential antioxidant capacity and exhibit efficiently self-activated DNA cleavage activity and hydroxyl radical ROS cleavage mechanism. MTT was used to assay cytotoxicity of the complexes toward a series of tumor cell and HUVEC cell lines. Both complexes exhibited higher selective cytotoxicity against tumor cells than normal cells, which is significantly superior to the positive control. The complexes could induce cell apoptosis via mitochondrial pathway by increase of ROS levels and decrease of mitochondrial membrane potential (Δψ m ). Furthermore, the complexes can obviously inhibit the migration and invasion of the quiz tumor cells, as well as inhibit the angiogenesis of HUVEC cells.
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Journal of Orthopaedic Surgery and Research
·
2023
·Vol. 18
·期 1
·1-15
·
IF 2.6
Sun Kai,Xue Yuman,Zhang Xin,Li Xiaodong,Zhao Jun,Xu Xilin,Zhang Xiaofeng,Yang Fubiao
Background The impaired blood supply to the bones is an important pathological feature of steroid-induced osteonecrosis of the femoral head (SIONFH). Danshen is a Chinese herb that shows therapeutic effects on SIONFH, but the effects of one of its major bioactive constituents, Tanshinone I (TsI), on SIONFH remain unknown. Here, we evaluated the effects of TsI on SIONFH, particularly focusing on its effects on angiogenesis, in in vivo and in vitro research. Methods SIONFH was induced in Sprague–Dawley rats by an intramuscular injection of methylprednisolone (40 mg/kg) in combination with an intraperitoneal injection of lipopolysaccharide (20 μg/kg). Morphological alterations of the femoral head were observed by dual-energy X-ray absorptiometry and HE staining. Western blot, qRT-PCR, and immunohistochemical/immunofluorescence staining were used to determine gene expression. Results TsI (10 mg/kg) alleviated bone loss and rescued the expression of angiogenesis-related molecules (CD31, VWF, VEGF, and VEGFR2) in the femoral heads of SIONFH rats. Notably, TsI rescued the down-regulated expression of SRY-box transcription factor 11 (SOX11) in CD31 + endothelial cells in the femoral heads of SIONFH rats. In vitro studies showed that TsI preserved the dexamethasone-harmed angiogenic property (migration and tube formation) of human umbilical vein cells (EA.hy926), suppressed dexamethasone-induced cell apoptosis, reduced pro-apoptotic proteins (cytosolic cytochrome C, Bax, and caspase 3/9) and increased anti-apoptotic protein Bcl-2, whereas silencing of SOX11 reversed these beneficial effects. Conclusions This study demonstrates that TsI alleviates SIONFH and promotes angiogenesis by regulating SOX11 expression. Our work would provide new evidence for the application of TsI to treat SIONFH. Graphical Abstract
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INORGANIC CHEMISTRY
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2022
·
IF 5.436
Qiang Huang,Changqing Tian,Junan Lai,Fei Qi,Nan Zhang,Jiaxin Liu,Zhiyu Liang,Daofu Wu,Xiaosheng Tang
In response to calling for a sustainable and carbon-neutral economy, the conversion of CO2 to useful chemicals using the solar energy is a potential tactic to relieve the global energy dilemma and environmental issues, which has been a hot topic so far. Recently, the lead halide perovskites as novel photocatalysts have attracted researchers’ interests. However, they generally encounter poor stability and lead toxicity, restricting their large-scale practical applications. Here, the lead-free Cs2TeX6 (X = Cl, Cl0.5Br0.5, Br, Br0.5I0.5, and I) perovskite microcrystals with strong stability were prepared and used to realize the CO2 photocatalytic reduction efficiently. The prepared Cs2TeBr6 microcrystals delivered stronger photocatalytic ability than many previously reported photocatalysts, with the CO and CH4 yields of 308.63 and 60.42 μmolg–1, respectively, under 3 h of illumination. The presented strategy in our work provides new ideas of designing and preparing efficient and practical CO2 reduction photocatalysts based on nonleaded and high-stability halide perovskites.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2022
·
IF 5.518
Zewei Liang,Jing Wen,Yuanliang Zhou,Tingting Liu,Jinmei Dong,Weixin Zheng,Chenggong Chang,Xueying Xiao,Qiyuan Liu,Xiaogang Zheng
BiOCl 0.5 X 0.5 (X = F, Br, and I) heterojunctions were prepared via the solvothermal approach to achieve the solar-light induced photodegradation of tetracycline hydrochloride (TCH). The photocatalytic activity of BiOCl coupling with BiOI is greatly affected by the molar ratio of Cl/I. The best photocatalytic efficiency of 97.9 % within 120 min is obtained by flower-like BiOCl 0.5 I 0.5 microspheres with a Cl/I molar ratio of 1:1, and the removal efficiency of TCH over BiOCl 0.5 I 0.5 declines to 93.2 % after five cycles. Compared with BiOCl 0.5 Br 0.5 and BiOCl 0.5 F 0.5 , BiOCl 0.5 I 0.5 presents the better photocatalytic activity . Z-scheme junction between BiOCl and BiOI is favorable for the efficient separation and migration of photo-generated charge carriers, and the narrow band-gap structure of BiOCl 0.5 I 0.5 microspheres favors the visible-light harvesting.·O 2 - plays a vital role in BiOCl 0.5 I 0.5 -assisted photocatalytic reaction. However, the change in valance states induced by photo-corrosion is responsible for the solar-light deactivation of BiOCl 0.5 I 0.5 .
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 12
·6588
·
IF 6.208
Jiafeng Niu,Ruxue Yan,Juan Shen,Xiaoyu Zhu,Fanqiang Meng,Zhaoxin Lu,Fengxia Lu
Type I L-asparaginase fromBacillus licheniformisZ-1 (BlAase) was efficiently produced and secreted inBacillus subtilisRIK 1285, but its low yield made it unsuitable for industrial use. Thus, a combined method was used in this study to boost BlAase synthesis inB. subtilis. First, fifteen single strong promoters were chosen to replace the original promoter P43, with PyvyD achieving the greatest BlAase activity (436.28 U/mL). Second, dual-promoter systems were built using four promoters (PyvyD, P43, PaprE, and PspoVG) with relatively high BlAase expression levels to boost BlAase output, with the engine of promoter PaprE-PyvyD reaching 502.11 U/mL. The activity of BlAase was also increased (568.59 U/mL) by modifying key portions of the PaprE-PyvyD promoter. Third, when the ribosome binding site (RBS) sequence of promoter PyvyD was replaced, BlAase activity reached 790.1 U/mL, which was 2.27 times greater than the original promoter P43 strain. After 36 h of cultivation, the BlAase expression level in a 10 L fermenter reached 2163.09 U/mL, which was 6.2 times greater than the initial strain using promoter P43. Moreover, the application potential of BlAase on acrylamide migration in potato chips was evaluated. Results showed that 89.50% of acrylamide in fried potato chips could be removed when combined with blanching and BlAase treatment. These findings revealed that combining transcription and translation techniques are effective strategies to boost recombinant protein output, and BlAase can be a great candidate for controlling acrylamide in food processing.Keywords:L-asparaginase;Bacillus subtilis;promoter;dual-promoter systems;ribosome binding site;scale-up fermentation;acrylamide
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 11
·6326
·
IF 6.208
Ting Sun,Xian Li,Xiaochuan Jin,Ziyi Wu,Xiachao Chen,Jieqiong Qiu
Graphene oxide is well known for its excellent fluorescence quenching ability. In this study, positively charged graphene oxide (pGO25000) was developed as a fluorescence quencher that is water-soluble and synthesized by grafting polyetherimide onto graphene oxide nanosheets by a carbodiimide reaction. Compared to graphene oxide, the fluorescence quenching ability of pGO25000 is significantly improved by the increase in the affinity between pGO25000 and the DNA strand, which is introduced by the additional electrostatic interaction. The FAM-labeled single-stranded DNA probe can be almost completely quenched at concentrations of pGO25000 as low as 0.1 μg/mL. A simple and novel FAM-labeled single-stranded DNA sensor was designed for Hg2+detection to take advantage of exonuclease I-triggered single-stranded DNA hydrolysis, and pGO25000 acted as a fluorescence quencher. The FAM-labeled single-stranded DNA probe is present as a hairpin structure by the formation of T–Hg2+–T when Hg2+is present, and no fluorescence is observed. It is digested by exonuclease I without Hg2+, and fluorescence is recovered. The fluorescence intensity of the proposed biosensor was positively correlated with the Hg2+concentration in the range of 0–250 nM (R2= 0.9955), with a seasonable limit of detection (3σ) cal. 3.93 nM. It was successfully applied to real samples of pond water for Hg2+detection, obtaining a recovery rate from 99.6% to 101.1%.Keywords:positively charged graphene oxide (pGO);exonuclease I;fluorescence quencher;hairpin structure;T–Hg2+–T
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BIOMATERIALS
·
2022
·
IF 15.304
Yuyu Zhao,Long Bai,Yi Zhang,Runhua Yao,Yonghua Sun,Ruiyue Hang,Xiangyang Chen,Huaiyu Wang,Xiaohong Yao,Yin Xiao,Ruiqiang Hang
Osseointegration of implants is a complex physiological process that requires temporal and spatial regulation of immune responses, angiogenesis , and osteogenesis. To achieve efficient and long-term osseointegration, type I collagen (COL1) decorated nanoporous network was developed on titanium substrates via alkali treatment , polydopamine coating, and layer-by-layer (LBL) self-assembly. It was noted that the simple physisorbed COL1 could be easily desorbed from the nanostructured surface , however, multilayer COL1 constructed by polydopamine and LBL self-assembly obscured the nanoporous network of the alkali-treated titanium surfaces. Interestingly, the nanostructured surface covalently immobilized with COL1 (T-ADC) could timely convert macrophages (MΦs) from pro-inflammatory M1 to pro-healing M2 phenotype, generating a beneficial osteoimmune microenvironment and promoting angio/osteo-genesis. RNA sequencing revealed that the nanostructure and COL1 could synergistically activate RhoA/ROCK, PI3K-AKT, and classical MAPK signaling pathways in MΦs to sustain the cell cycle, and trigger autocrine feedback-mediated JAK-STAT and FoxO signaling pathways, which in turn motivated autophagy and oxidative stress resistance and attenuated lipopolysaccharide-induced Toll-like receptor signaling pathway and its downstream NF-κB and JNK/p38 MAPK signaling cascades, leading to the inhibition of inflammation and osteoclastic-related gene expression of MΦs. Simultaneously, T-ADC prominently facilitated angiogenesis of endothelial cells and osteogenesis of osteoblasts as well as their cross-talks, further highlighting synergistically positive effects of the nanostructure and COL1 on osseointegration. In vivo experiments revealed that T-ADC could induce abundant new bone mass and ameliorative osseointegration, corroborating the in vitro results. The study elucidated that the COL1 decorated nanoporous network on titanium surfaces could significantly regulate early inflammatory reaction and subsequent angio/osteo-genesis processes, resulting in favorable osseointegration.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2022
·
IF 9.136
Chuankun Yin,Aimal Khan,Qiaohui Gao,Quan Li,Xiaoyun Zhou,Xiuying Liu,Aihua Xu,Xiaoxia Li
Recently, the development of efficient mixed metal oxide catalysts for organic pollutants degradation via peroxymonosulfate (PMS) activation is a hot topic in the area of wastewater treatment . In this work, the silver-copper mixed-oxide, Ag 2 Cu 2 O 3 (ACO) was reported for the first time as a highly active catalyst for removing organic pollutants in the presence of PMS. ACO was successfully synthesized from Ag + and Cu 2+ ions at 60 °C, and its structure was strongly influenced by the temperature. The catalyst could almost completely remove p -nitrophenol and other pollutants with 0.65 mM PMS after 20 min, and also exhibited satisfactory stability and recyclability. The system efficiency was shown to vary with the concentration of PMS and catalyst, as well as solution pH. Scavenger experiment and EPR analysis indicated that radicals produced by the oxidation of Ag(I) and Cu(I) with PMS could promote degradation reaction. The synergistic effect between Cu and Ag in the bimetallic catalyst was demonstrated to contribute to the high performance: Cu(II)-O-Ag(I) bonds promote the conversion of Cu(II) species to Cu(I) through reaction with PMS, and facilitate the oxidation of Cu(I) and Ag(I) by PMS to generate active radicals. The study provides a feasible strategy to design efficient and stable bimetal oxide catalysts for PMS activation.
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