BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
·
2021
·Vol. 69
·期 6
·2561-2572
·
IF 2.431
Yongjun Xia,Caiyun Yang,Xiaofeng Liu,Guangqiang Wang,Zhiqiang Xiong,Xin Song,Yijin Yang,Hui Zhang,Lianzhong Ai
There have been many studies on the activities and polysaccharide production of Sanghuangporus vaninii . However, few studies have looked at triterpene production from S. vaninii using liquid-state fermentation. A method for enhancing the production of triterpenes by in situ extractive fermentation (ISEF) was studied. Eight solvents were investigated as extractants for triterpene production in the ISEF system. The results showed that using vegetable oil as an extractant significantly increased the yield of total triterpenes and biomass of S. vaninii YC-1, reaching 18.98 ± 0.71 and 44.67 ± 2.21 g/L, respectively. In 5 L fermenter experiments, the added vegetable oil improved the dissolved oxygen condition of the fermentation broth and promoted the growth of S. vaninii YC-1. Furthermore, adding vegetable oil increased the expression of fatty acid synthesis-related genes such as FAD2 and SCD , thereby increasing the synthesis of unsaturated fatty acids in the cell membrane of S. vaninii YC-1. Therefore, the cell membrane permeability of S. vaninii YC-1 increased by 19%. Our results indicated that vegetable oil increased the permeability of S. vaninii YC-1 cell membranes to promote the production of total triterpenes. The use of vegetable oil as an extractant was thus effective in increasing the yield of triterpenes in the ISEF system.
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高影响因子
CATALYSIS LETTERS
·
2021
·Vol. 152
·期 9
·2832-2839
·
IF 3.186
Zhang Fengming,Li Ran,Zhang Jiapeng,Dong Hua
Dendritic fibrous KCC-1 supported CuCo bimetal catalysts (CuCo/KCC-1) are fabricated by coprecipitating Cu 2+ and Co 2+ on the surface of KCC-1 nanospheres. The microstructure and composition of the fabricated CuCo/KCC-1 bimetal catalysts are analyzed with ICP-OES, SEM, TEM, and XPS. The CuCo/KCC-1 catalysts demonstrate excellent performance in catalyzing ammonia borane hydrolysis to produce hydrogen in alkaline solutions. As an example, the hydrolysis of ammonia borane catalyzed by Cu 3 Co 1 /KCC-1 (with a Cu/Co molar ratio about 3:1) gives a hydrogen generation rate of 5.43 L/(min g metal ), and an activation energy of 23.96 kJ/mol. The synergic effect between Cu and Co in the CuCo/KCC-1 catalysts maintains the excellent catalytic activity while reducing the usage of Co, making it possible to decrease the cost of the catalyst. Graphical Abstract
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CHEMISTRY & BIODIVERSITY
·
2021
·Vol. 19
·期 2
·e202100735
·
IF 2.408
Zhipeng Qi,Xianyu Fan,Chunyi Zhu,Dongsheng Chang,Jianjun Pei,Linguo Zhao
Synthesis of β-ionone in microbial cell factories is limited by the efficiency of carotenoid cleavage dioxygenases (CCDs). To obtain genes responsible for specific cleavage of carotenoids generating β-ionone, a novel carotenoid cleavage dioxygenase 1 from Morus notabilis was cloned and overexpressed in Escherichia coli . The MnCCD1 protein was able to cleave a variety of carotenoids at the positions 9, 10 (9′, 10′) to produce β-ionone, 3-hydroxy-4-oxo-β-ionone, 3-hydroxy-β-ionone, and 3-hydroxy-α-ionone in vitro . MnCCD1 could also cleave lycopene and β-carotene at the 9, 10 (9′, 10′) bind bond to produce pseudoionone and β-ionone, respectively, in E. coli accumulating carotenoids. The enzyme activity of MnCCD1 was reached 2.98 U/mL at optimized conditions (temperature 28 °C, IPTG 0.1 mM, induction time 24 h). The biochemical characterization of MnCCD1 revealed the optimal activities were at pH 8.4 and 35 °C. The addition of 10 % ethanol could increase enzyme activity at above 15 %. However, an obvious decline was observed on enzyme activity as the concentration of Fe 2+ increased (0–1 mM). The V max for β-apo-8′-carotenal was 72.5 U/mg, while the K m was 0.83 mM. The results provide a foundation for developing the application of carotenoid cleavage dioxygenases as biocatalysis and synthetic biology platforms to produce volatile aroma components from carotenoids.
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高影响因子
MICROPOROUS AND MESOPOROUS MATERIALS
·
2021
·
IF 5.455
Xu Wang,Qing You,Yushuai Wu,Chenyao Bi,Huiyong Chen,Chengyi Dai,Qingqing Hao,Jianbo Zhang,Xiaoxun Ma
A novel hierarchical tungsten-substituted Silicalite-1 zeolite with the highly interconnected hollow structure (HWS-1) was synthesized for the first time by post-treating the purely microporous tungsten-substituted Silicalite-1 (WS-1) through simultaneous desilication and tungstation, in which a combination of sodium hydroxide and tetrapropyl ammonium hydroxide (TPAOH) was used to achieve a systematic balance between dissolution and recrystallization and further promote the synchronous tungstation with using sodium tungstate dihydrate as a tungsten source. Under an optimum addition ratio of NaOH/TPAOH, the resulted HWS-1 exhibited not only well retained microporosity and crystallinity but also obviously enlarged external surface area and mesopore volume in contrast to the parent WS-1. Furthermore, the effective tungstation also facilitated the substitution and increased the amount of coordinated W species in zeolite frameworks. The catalytic performance of HWS-1 was evaluated by the epoxidation of cyclohexene with using H 2 O 2 as oxidant. Benefited by the highly interconnected hollow structure and more accessible active sites on the enlarged external surface, the HWS-1 catalyst revealed a higher catalytic activity with improved cyclohexene conversion than WS-1. Moreover, it was found that a significant positive effect on promoting epoxidation was achieved by tungsten-substituted Silicalite-1 (WS-1 and HWS-1) catalysts reflecting at a much higher selectivity of epoxide (>79%) in comparison with titanium-substituted Silicalite-1 (TS-1) (~50% selectivity of epoxide), due to their superior hydrophobicity and suitably weaker acidity, which further demonstrated the potential application of the developed hierarchical tungsten-substituted Silicalite-1 as a candidate catalyst for epoxidation and other selective oxidation reactions.
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MOLECULES
·
2021
·Vol. 26
·期 11
·3064
·
IF 4.412
Qing Shi,Ling-Yi Shen,Hong Xu,Zhi-Yong Wang,Xian-Jiong Yang,Ya-Li Huang,Carl Redshaw,Qi-Long Zhang
A novel 1-hydroxy-2,4-diformylnaphthalene-based fluorescent probeLwas synthesized by a Knoevenagel reaction and exhibited excellent sensitivity and selectivity towards sulfite ions (SO32−) and bisulfite ions (HSO3−). The detection limits of the probeLwere 0.24 μM using UV-Vis spectroscopy and 9.93 nM using fluorescence spectroscopy, respectively. Furthermore, the fluorescent probeLcould be utilized for detection in real water samples with satisfactory recoveries in the range 99.20%~104.30% in lake water and 100.00%~104.80% in tap water by UV-Vis absorption spectrometry, and in the range 100.50%~108.60% in lake water and 102.70%~103.80% in tap water by fluorescence spectrophotometry.Keywords:fluorescent probe;sulfite/bisulfite;crystal structure;real sample detection
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MICROPOROUS AND MESOPOROUS MATERIALS
·
2021
·
IF 5.455
Rong Pan,Yining Tang,Yanni Guo,Jun Shang,Lei Zhou,Wei Dong,Deliang He
To develop effective SO 2 adsorbents, HKUST-1 and HKUST-1 @ GO were synthesized by hydrothermal and room-temperature methods, and characterized by SEM, BET, XRD , TG and FTIR. The preparation method of the adsorbent, amount of adsorbent, initial concentration of SO 2 , and the influence of time and temperature on the adsorption of SO 2 by HKUST-1 and HKUST-1@GO were studied. The results showed that the hydrothermally synthesized HKUST-1@1.2%GO was the best adsorbent for SO 2 , the SO 2 concentration did not affect the final adsorption efficiency, the best adsorption time was 20 min, and the best adsorbent dose was 200 mg. The kinetics and isotherms of HKUST-1@1.2%GO were studied, and the thermodynamic parameters were calculated. The static SO 2 adsorption of HKUST-1@1.2%GO conformed to the pseudo-first-order model and Freundlich model, and was a spontaneous exothermic process. Lowering the temperature is beneficial to the adsorption. At 288 K, the maximum theoretical SO 2 adsorption capacity of HKUST-1 @1.2% GO was 72.49 mmol g −1 . After five adsorption cycles, the adsorption efficiency only decreased by 0.8%, indicating that the adsorbent has excellent repeatability and cyclic stability.
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MOLECULAR AND CELLULAR BIOCHEMISTRY
·
2021
·Vol. 476
·期 6
·2479-2489
·
IF 3.396
Chai Qian,Meng Ziang,Lu Dexue,Zhang Ziying,Liu Meili,Wu Weihua
Cardiomyocyte death is an important pathogenic process in cardiac complications of diabetes. Diabetic patients often suffer glycemic variability. Pyroptosis is a form of programmed cell death triggered by inflammasomes and related with caspase-1 and gasdermin D activation. The present study was designed to examine the effects of intermittent high glucose simulating glycemic variability on the pyroptosis of cardiomyocytes in vitro. Rat H9C2 cardiomyocytes were incubated with normal glucose (NG), constant high glucose (CHG) and intermittent high glucose (IHG). Results showed that compared to CHG treatment, IHG further inhibited cell proliferation and promoted cell death of H9C2 cardiomyocytes. In addition, IHG upregulated higher levels of the expressions of inflammasome NLR family pyrin domain containing 3 (NLRP3) and adaptor protein apoptosis-associated speck-like protein containing CARD domain (ASC) and increased higher levels of activated caspase-1 and gasdermin D than CHG treatment. Moreover, the production of reactive oxygen species (ROS) and activation of NF-κB that is induced by IHG were significantly higher than that induced by CHG. Knockdown of sodium–glucose cotransporters 1 (SGLT1) in H9C2 cardiomyocytes was performed and the effects of SGLT1 on IHG-induced pyroptosis was evaluated. The results demonstrated that knockdown of SGLT1 partially reduced IHG-induced pyroptosis, ROS generation and NF-κB activation. Our results indicated that IHG is harmful to cardiomyocytes and it might be partially because of the SGLT1-depedent pyroptosis in cardiomyocytes.
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高影响因子
Catalysts
·
2020
·Vol. 10
·期 11
·1304
·
IF 3.52
Qiang Yuan,Jifeng Pang,Wenguang Yu,Mingyuan Zheng
A high-performance Pt catalyst supported on SBA-15 was developed for furfural decarbonylation. Compared to Pt catalysts loaded on microporous DeAl-Hbeta zeolite and hierarchical micro-mesoporous MFI nanosheet (NS) materials, the 1%Pt/SBA-15 catalyst afforded notably higher activity, furan selectivity and stability owing to the negligible acid sites and proper mesopores on the SBA-15 support. Among a set of 1%Pt/SBA-15 catalysts bearing Pt nanoparticles (NPs) with sizes of 2.4–4.3 nm, the catalyst with 3.7 nm Pt NPs afforded the highest furan selectivity. Over the optimal catalyst, 88.6% furan selectivity and ca. 90% furfural conversion were obtained at 573 K and a high weight hourly space velocity (WHSV) of 16.5 h−1. Moreover, the reaction temperatures at 440–573 K and the ratios of H2to furfural at 0.79–9.44 did not affect the reaction selectivity notably, showing that the reaction over 1%Pt/SBA-15 can be conducted over a wide range of conditions. The catalyst was stable under the harsh reaction conditions and lasted for 90 h without significant deactivation, demonstrating the superior property of SBA-15 as a catalyst support for furfural decarbonylation.Keywords:decarbonylation;furfural;furan;platinum;SBA-15
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高影响因子
ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Zhenya Yu,Xue Chen,Ying Cheng,Hongmei Yang,Fang Wang,Zilin Chen
A label-free electrochemical method was developed for sensitive determination of ten-eleven translocation protein 1 (TET1) which can mediate the demethylation of DNA . This strategy is mainly based on MspI-mediated restriction endonuclease reaction. Current response difference of the biosensor before and after cleavage by MspI was dependent on the activity and concentration of TET1. With the aid of Au nanoparticles , this method shows a good linear range from 0.0042 μg μL −1 to 0.0210 μg μL −1 with a correlation coefficient of 0.9350 and a low limit of detection 0.00098 μg μL −1 . Finally, this method was used to investigate the effects of n-oxalylglycine (NOG) and taxol on activity of TET1. The results indicated that NOG could inhibit TET1 activity but taxol could not. So this electrochemical biosensor could be applied to TET activity evaluation and inhibitor screening in field of biomedicine and clinical diagnosis.
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高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2020
·Vol. 31
·期 13
·10639-10648
·
IF 2.22
Si Shufang,Deng Hongmei,Wang Tiantian,Zheng Dongliang,Yang Pingxiong,Chu Junhao
Multiferroic ferrite–ferroelectric composites with general formula x CoFe 2 O 4 -(1 − x )K 0.5 Bi 0.5 TiO 3 ( x = 0.0, 0.3, 0.5, 0.7, and 1.0) ( x CFO-KBT) were developed by conventional solid-state reaction method. The structural, optical, magnetic and ferroelectric properties for the prepared samples were studied in detail. Two individual structures of spinel-type ferrite and tetragonal-type ferroelectric are characterized through X-ray diffraction while Raman scattering spectra and SEM analyses furtherly confirm the coexistence of the two phases in the composites. The bandgaps calculated for the composites narrow dramatically compared with the pure KBT, which are attributed to Co 2+ d–d on-site transitions from the CFO phase. The magnetic property transforms from diamagnetism for pure KBT to ferromagnetism for the composites and pure CFO with saturation magnetization M s increasing gradually with addition of CFO. Besides, the room temperature ferroelectricity has been detected in the composites, which turns out to be enhanced by space charge contribution of CFO phase. The improvement of optical and multiferroic properties in the composites indicates great prospect on visible-light optical devices and non-volatile data storage.
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Chinese Journal of Natural Medicines
·
2020
·
IF 2.014
Li YAO,Yu-Xia YANG,Hui CAO,Huan-Huan REN,Zheng NIU,Ling SHI
Osthole is observed to have the capacity to treat pulmonary arterial hypertension (PAH) in rats, but molecular mechanism is still unknown. The present study aims to discover therapeutic targets and explore therapeutic mechanism of osthole against PAH from metabolic perspective. A rat model with PAH was successfully established with MCT, following osthole administration, then untargeted metabolomics assay was performed using UPLC-Q-TOF-MS to identify differential metabolites and associated metabolic pathways, at last mechanism investigation was done by qRT-PCR, Western blot and ELISA. Differential metabolites characterized in rats with PAH were mostly assigned to sphingolipid metabolism, synthesis of unsaturated fatty acids, glycolysis, nucleotide metabolism, steroid hormone biosynthesis. Furthermore, osthole reversed high level of S1P by modulating metabolic enzyme Sphk1 in rats with PAH. In addition, osthole inhibited the expression of Sphk1 by downregulating microRNA-21, phosphorylation of Akt, phosphorylation of mTOR in vivo and in vitro . These results demonstrated that metabolomics is a promising approach to discover potential drug target for PAH treatment. Importantly, our findings further elucidated therapeutic mechanism of osthole, a natural product, having a role of metabolic regulator to potentially treat PAH by targeting inhibition of Sphk1/S1P via microRNA-21-PI3K/Akt/mTOR signal pathway. Altogether, this discovery paves a critical foundation for enabling osthole to be a candidate compound to treat PAH.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Yong Liu,Ensheng Xu,Chao Xu,Wei Wei
As a tumor-related enzyme, PARP-1 activity is closely related to the occurrence of cancer. Sensitive detection of PARP-1 activity is of significance in the early diagnosis and treatment of cancer. Therefore, the gold nanorods (AuNRs) based biological nanoprobe was constructed for the visual and label-free detection of PARP-1 activity. dsDNA which activate PARP-1 was modified on AuNPs ( [email protected] ). MoO 4 2− is added to catalyze H 2 O 2 and KI to accelerate the etching of AuNRs. In the presence of PARP-1, hyperbranched poly (ADP-ribose) polymers (PAR) formed on the surface of [email protected] ( [email protected] @PAR). A large number of PO 4 3- of the PAR reacted with MoO 4 2− to generate PMo 12 O 40 3- . Thus, PO 4 3- diminished MoO 4 2− concentration in solution, preventing the etching of AuNRs. Therefore, PARP-1 prevented the etching of AuNRs. With the increasing concentration of PARP-1, the color of the solution changed from light blue to brown obviously. The method was applied to assay actual samples with satisfactory results. The strategy has the advantages of rapid quantitative analysis of PARP-1 activity with naked eyes, simple operation and good application prospect.
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