JOURNAL OF FLUORESCENCE
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2024
·
IF 2.6
Chen Xian,Zhang Menghan,Zheng Lang,Deng Xiaoqin,Chen Qiang,Han Wendi,Huang Zhengjun,Weng Shaohuang
The establishment of a convenient and efficient testing method is crucial and needed for the monitoring of diosmin. In this study, nitrogen doped carbon dots (N-CDs) with the particle size distribution of 2.5–5.7 nm and the average diameter of 4.1 nm were successfully synthesized using a simple strategy. N-CDs exhibited excellent and stable fluorescence performance with the quantum yield of 22.33%. Correspondingly, a fluorescence analytical method was developed for diosmin determination using N-CDs as probe. UV-vis absorbance spectroscopy and the evaluation of internal filtering parameters verified that the mechanism causing the quenching of N-CDs was an internal filtering effect (IFE). The concentration of diosmin can be directly evaluated based on the quenched fluorescence intensities. After optimizing experimental conditions, it was found that the fluorescence quenching efficiency ((F 0 -F)/F 0 ) of N-CDs exhibited a good linear relationship with the concentration of diosmin (C Diosmin ) in the range of 3.0–50 µg/mL. The limit of detection (LOD) was 0.86 µg/mL based on 3σ/slope ( n = 13). This method was successfully applied to accurately determine the content of diosmin in diosmin tablets and human plasma samples with good reproducibility. It stands out for its simplicity, speed, and acceptable determination performance.
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高影响因子
FUEL
·
2024
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IF 6.7
Tingwei Zhang,Yongcan Jin,Huining Xiao
Sulfonated carbon/γ-Al 2 O 3 composites (AlSC-x, x = 1, 2, 3) were successfully prepared by the three-step method of impregnation-carbonization-sulfonation. The introduction of sulfonated sucrose-based carbon onto nano-γ-Al 2 O 3 enhanced its Brønsted acidity and weakened the original Lewis acidity. The Brønsted/Lewis acidity of the composite catalysts can be regulated by controlling the sulfonated carbon intake. AlSC-1 containing suitable Brønsted/Lewis acidity showed the best catalytic performance for converting glucose to 5-hydroxymethylfurfural (HMF). In monophasic DMSO/H 2 O (2.0/0.2 mL), an HMF yield of 62.3 % was achieved at 160 °C and 4.0 h when 0.02 g of glucose and 0.02 g of AlSC-1 were used. Adding a moderate amount of water into DMSO is crucial to improve HMF selectivity, and the DMSO/H 2 O system exhibited superior performance to other solvent systems, such as pure water, 1,4-dioxane/H 2 O, and GVL/H 2 O. More importantly, the recyclability of AlSC-1 was pretty good. In addition, AlSC-1 also exhibited much better performance than the other composite catalysts, including sulfonated carbon/nano-TiO 2 , sulfonated carbon/nano-ZrO 2 , and sulfonated carbon/nano-SnO 2 .
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SEPARATION AND PURIFICATION TECHNOLOGY
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2024
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IF 8.1
Jiachang Shen,Maohua Yang,Jianmin Xing
Utilization of an in vitro multi-enzyme catalytic system for the synthesis of fructose from carbon dioxide is a promising approach towards achieving carbon neutrality. However, the lack of effective separation methods for the buffer HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) and the product fructose within the system has hindered the industrialization of in vitro multi-enzyme catalytic synthesis systems. This study aimed to separate and recover HEPES and fructose using nanofiltration technology. We investigated the influence of various parameters, including total solution concentration, solute concentration ratio, pH, and temperature, on the nanofiltration separation performance. Experimental results revealed that increasing the pH to 9.0 significantly decreased the fructose retention rate while maintaining a nearly complete HEPES retention rate. The HEPES-fructose mixed solution was further subjected to diafiltration using a spiral-wound membrane, ultimately yielding fructose and HEPES solutions with purities of 90.22 % and 92.93 %, respectively. Combining separation test results with models such as DSPM-DE, and using characterization methods such as ATR-FTIR and pore size distribution analysis, we observed pore swelling during nanofiltration of the HEPES-fructose solution at pH 9.0. A mechanism for this pore swelling, induced by the dissociation of HEPES under the influence of pH, was proposed: Dissociated HEPES in the solution interacts with the membrane surface at the liquid-membrane interface, causing reversible swelling of the membrane pores at the membrane surface, thereby reducing the retention rate of fructose. The nanofiltration process developed in this study effectively separates HEPES and fructose, enabling resource recycling and cost reduction. Furthermore, this study provides theoretical foundations and practical guidance for the design of nanofiltration separation strategies for similar systems using HEPES as a buffer, holding significant scientific and practical value.
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Horticulturae
·
2024
·Vol. 10
·期 6
·587
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IF 3.1
Jiangmei Hu,Junhuo Cai,Xinrui Hu,Lijun Wang,Qiangqiang Cheng,Xiuhua Tao
Turpinia argutais an excellent medicinal plant mainly used for the treatment of pharyngitis, tonsillitis, and tonsillar abscesses. However, an efficient regeneration protocol using tissue cultures forT. argutadoes not exist. Its main medicinal constituents are flavonoids, particularly ligustroflavone and rhoifolin. Here, we aimed to establish a tissue culture system forT. argutafor the first time using annual stem segments with axillary buds harvested from the field of the Jiangxi Academy of Forestry as explants by dynamically determining the accumulation of effective functional components in the tissue culture plantlets. Orthogonal tests were conducted to compare the effects of different explant disinfection times, media, and exogenous hormone ratios on the induction of the axillary bud growth, successional proliferation, and rooting ofT. argutastem segments. The best explant disinfection effect was achieved by disinfecting theT. argutaexplant with 75% ethanol for 50 s, followed by 0.1% mercuric chloride (HgCl2) for 6 min, and the optimal media for successional proliferation and rooting were Murashige and Skoog (MS) + 0.2 mg/L of 6-benzyladenine (6-BA), + 0.03 mg/L of naphthaleneacetic acid (NAA), and ½ MS + 2.5 mg/L of indole-3-butyric acid + 0.5 mg/L of NAA, respectively. The detection of ligustroflavone and rhoifolin in tissue culture plantlets 0, 3, and 5 months after transplanting showed a significant increasing trend and eventually exceeded the content requirements of the 2020 Edition ofChinese Pharmacopoeia forT. arguta. Our findings provide, for the first time, an effective tissue culture system forT. arguta, thereby providing important information to support the germplasm preservation, innovation, and application ofT. argutain the future.
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MICROCHEMICAL JOURNAL
·
2024
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IF 4.9
Yao Jin,Xiangwei Yuan,Junchun Guo,Li Xu,Qian Wang,Xiaoli Xiong
Dopamine (DA) is a central neurotransmitter that plays a crucial role in human metabolism. Its precise detection is of great importance in disease diagnosis. Compared with single-signal detection methods, colorimetric and electrochemical dual-signal outputs can self-validate the measured data and counteract interference effects, which is expected to provide more reliable detection results. In this paper, carbon nanofiber-supported 3D bristlegrass-like π-conjugated conducting metal–organic frameworks ( c MOF) Cu 3 (HHTP) 2 nanorod arrays (Cu 3 (HHTP) 2 NRAs/CNF) catalyst with core–shell structure are synthesized by electrospinning and solvothermal reaction. The obtained catalyst possesses remarkable peroxidase-like activity which can catalyze the colorless 3,3′,5,5′ −tetramethylbenzidine (TMB) to blue oxTMB. The mechanism study showes that ·OH radicals are the main active substances. Meanwhile, the core–shell structure between π-conjugated cMOF and CNF can effectively inhibit the aggregation of Cu 3 (HHTP) 2 nanorods and generate more catalytic active sites, resulting in strong electrocatalytic activity of Cu 3 (HHTP) 2 NRAs/CNF. As a result, a novel dual-mode sensor based on colorimetric and electrochemical are constructed for detecting dopamine (DA) with a good linear relationship ranging from 2.5-60 μM and 0.1–106 μM, and the limit of detection (LOD) are 0.27 μM and 10.1 nM (S/N=3), respectively. This study opens up a new idea for the application of c MOF peroxidase-like enzyme in sensing field.
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Analytical Methods
·
2025
·
IF 2.6
Chen Lu,Jiayue Chen,Shengsheng Zhang,Yaru Tian,Xinyu Ying,Dan Zhang,Zhidan Luo,Xinxin Si,Ming Li
Two single nucleotide polymorphisms (SNPs) in the human SLCO1B1 gene, c.388A>G (rs2306283) and c.521T>C (rs4149056), are independent determinants of the efficacy and side effects of statin drugs. Multinational clinical guidelines recommend testing for SLCO1B1 genotypes before the initial use of statins. Current SLCO1B1 SNP identification methods, primarily based on quantitative fluorescence PCR, rely on expensive equipment, are time-consuming, and require cold-chain storage for reagents, making them unsuitable for use in resource-limited healthcare settings. In this study, we developed a CRISPR/Cas12b-based amplification-free genotyping technique for SLCO1B1 SNPs. Within 30 minutes of the isothermal reaction, genotyping of the c.388A>G and c.521T>C SNPs in the SLCO1B1 gene can be observed by the naked eyes under blue light. Additionally, maltodextrin was identified as an effective vitrification stabilizer for the CRISPR/Cas12b premix. A low-cost vitrification process was optimized to prepare a glass like solid reagent via room-temperature vacuum drying. The vitrified CRISPR/Cas12b reagent retained approximately 88% of its activity after 30 days of storage at 37 °C, eliminating the need for cold-chain storage and allowing for long-term preservation at room temperature. This vitrified CRISPR/Cas12b based rapid SNP detection technique is especially suitable for genotyping drug metabolism genes in primary healthcare settings, providing effective guidance for precision medicine in clinical practice.
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SOLID STATE SCIENCES
·
2024
·
IF 3.4
Ke Yang,Sisi Cheng,Ziqin Yao,Shijia Li,Yuting Yang
Researchers in the field of electrochemistry have recently become interested in the extraordinary features of ternary transition metal oxide nanostructures with spinel structure derived from metal-organic framework (MOFs). In this study, the zeolitic imidazolate framework (ZIF-67), which grows in situ on the surface of another metal organic framework (Ce-BTC), was created using the internal expansion growth method (IEGM), and the resulting complex was then used as a template to create the dumbbell-like nanocomposite. Due to the strong electron conduction of CeO 2 in composites, the electrode modified with composite material has good electrochemical activity in alkaline solution. As a glucose sensor, Co 3 O 4 /CeO 2 nanocomposite modified glass carbon electrode (Co 3 O 4 /CeO 2 /GCE) has better performance with a detection range of 83.75 μM–2796.84 μM, a reasonably good sensitivity of 790.746 μA mM −1 ·cm −2 , an estimated detection limit of 5.5 μA (S/N = 3) and a short response time less than 3s. The modified electrode can continue to function normally even when exposed to various interfering compounds for extended periods of time in the air. The successful use of this enzyme-free glucose sensor in fruit juice demonstrates its remarkable performance and provides ideas for future applications.
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ANALYTICAL LETTERS
·
2025
·
IF 1.8
Wanying Zhao,Yanqin Chen,Jun Tan,Yanru Bai,Fudong Ma
A method utilizing high-performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD) has been developed and validated for the simultaneous determination of raffinose, glucose, sucrose, and stachyose in waste liquid from cottonseed processing and in byproducts including cottonseed meal. The separation was performed using a carbohydrate ES column (250 × 4.6 mm, 5 μm) and isocratic elution with acetonitrile and water (70:30, v/v). The temperature of the column oven was maintained at 35 °C. The drift tube temperature for evaporative light scattering detection (ELSD) was operated 40 °C, while the nitrogen flow was set to 1.5 L·min−1. The saccharides were separated by HPLC-ELSD within 20 min. The coefficients of determination (R2 = 0.9986–0.9999) indicate that there was a linear relationship between saccharides and the response. The limits of detection were 0.004–0.023 mg·mL−1 and the quantification range was from 0.013 to 0.080 mg·mL−1. The intraday and interday precision were from 0.05 to 0.55% and 0.20 to 0.58%, respectively. The recovery was from 102.75 ± 2.03% to 106.76 ± 2.09% and the relative standard deviations were 1.87 to 1.98%. The method exhibited good stability in determining four saccharides in cottonseed processing waste liquid and the resultant byproduct cottonseed meal. This method exhibits high separation efficiency and sensitivity, excellent precision, and simplicity, which allows determination of the high value-added product, raffinose, in cottonseed processing waste liquid and meal.
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MONATSHEFTE FUR CHEMIE
·
2024
·
IF 1.7
Qiu Shengzhou,Wu Chenxi,Akter Sima,Hong Shaoli,Liu Huihong,Mahmud Sakil
Addressing the challenges of tuberculosis drug detection, this study investigates the application of copper(II) oxide modified screen-printed carbon electrodes (CuO/SPCEs) for the electrochemical detection of rifampicin, a critical drug in the treatment of tuberculosis and mycobacterial infections. The CuO/SPCE was fabricated via a constant potential electrodeposition technique and characterized using voltammetry. The bare SPCE surface exhibited a rough, porous structure, whereas the electrodeposition of CuO resulted in a smoother CuO/SPCE surface. The sensor exhibited high sensitivity with a detection limit of 2.89 μM for rifampicin and demonstrated excellent stability with a relative standard deviation of 3.5% over 10 measurements. The reproducibility was confirmed with an RSD of 4.2% across different electrodes. It also showed a linear response to rifampicin concentrations ranging from 10 to 200 μM. The real sample analysis showed a linear relationship between rifampicin concentration and peak current ( I = 0.739 + 0.008 c ), with a high correlation coefficient ( R 2 = 0.995). This electrochemical sensor’s simplicity, cost-effectiveness, and rapid response make it a promising tool for point-of-care applications in tuberculosis management. Graphical abstract
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Foods
·
2025
·Vol. 14
·期 3
·510
·
IF 5.1
Cuiping Zhang,Shujing Zhou,Chenxinzi Wu,Xinjian Xu,Xiangjie Zhu
Honey is a sweet substance laboriously collected and crafted from nectar by bees, and since ancient times, it has been deeply cherished by humans for its unique flavor and nutritional value. Litchi honey stands out among various types of honey with its unique flavor and sweet taste, and it is particularly favored by consumers. In accordance with the testing methodologies specified in relevant Chinese national standards, we conducted an exhaustive analysis of the physicochemical properties of six litchi honey samples in Southern China. The results showed that the moisture content fell within a range of 17.18% to 22.7%, while the electrical conductivity remained below 0.28 mS/cm, and amylase activity surpassed 7.7 mL/(g·h). The fructose content varied from 36.5% to 39.6%, with glucose content ranging between 30.57% and 37.63%. The combined total of these two monosaccharides was found to be within the spectrum of 69.63% to 77.23%, and sucrose content was recorded between 0.59% and 1.15%. The F/G was between 1.05 and 1.28, the proportion of fructose in reducing sugars ranged from 51.28% to 56.22%, and the maltose content was between 1.09% and 1.51%. The HMF content was measured between 1.04 and 3.49 mg/kg. Moreover, the presence of C-4 plant sugars was absent in all tested honey samples. These results definitively demonstrate that the physicochemical attributes of all litchi honey samples align with the standards set forth by Chinese national regulations and international authorities such as CODEX. During our in-depth examination of volatile constituents, we identified 26 common compounds, with trans-linalool oxide, linalool, lilac aldehyde B, lilac aldehyde D, α-terpineol, and cedrol emerging as pivotal in crafting the unique flavor and aroma profile of litchi honey. Additionally, the detection of methyl cyclosiloxane in litchi honey has garnered our attention, necessitating a comprehensive investigation into the honey production process. In conclusion, this study not only establishes a robust scientific basis for the quality assurance and product development of litchi honey but also provides valuable reference information for consumers in their selection of honey products.
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MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Miaomiao Song,Weihua Ma
Increasing attention has been directed towards oxidase mimics due to their catalytic ability toward enzyme substrates in the absence of H 2 O 2 , offering great colorimetric sensing potential in assays and diagnostics. However, slow electron-transfer and unstable absorbance over time often lead to unsatisfactory enzymatic reaction rates and unreliable readouts, significantly reducing detection efficiency and accuracy. Herein, we report on an Ag 6 Si 2 O 7 nanozyme utilizing 3,3′,5,5′-tetramethylbenzidine (TMB) as a chromogenic substrate, demonstrating excellent oxidase-like activity and long-term stable colorimetric signals. Experimental results reveal that the excellent catalytic kinetics of Ag 6 Si 2 O 7 (K m = 0.197 mM, Vmax = 5.1 × 10 −7 M/s) are attributed to ultrafast electron transfer between Ag + and TMB, mediated by Ag 2 O intermediates formed from self-released alkaline ions. Additionally, self-released H 2 SiO 4 2− improves the stability of the oxidized substrate, resulting in a long-term stable colorimetric signal (∼20 min), which exhibits reliable accuracy regardless of the time point at which analyte is added within 20 min, thus eliminating the interference of individual operation time errors. Ascorbic acid (AA) is selected as the target molecule to assess the performance of the Ag 6 Si 2 O 7 -TMB colorimetric sensing system. This approach demonstrates a wide detection range from 5 to 300 μM and a detection limit of 0.452 μM. The developed colorimetric sensing strategy exhibits good stability, specificity, rapidity, and potential application in real sample detection.
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ACS Sustainable Chemistry & Engineering
·
2024
·
IF 7.1
Zhifei Liu,Chunxiang Lu,Shuxia Yuan,Wei Cui,Shijie Wu,Xiaodan Ren,You Chen
As a prospective substitution for conventional lithium-ion batteries, lithium–sulfur batteries have been propelled into the spotlight on account of their merits, such as high energy density and ecofriendliness, among other things. Their commercialization, however, has run up against thorny impediments, with them primarily embodying sluggish evolution kinetics of sulfur species, the adverse shuttle effect of soluble polysulfide ions, and insufficient electronic conductivity of S8/Li2S2/Li2S. Herein, a multifunctional separator modifier based on porous carbonaceous microspheres inlaid with Fe3Se4 nanoparticles (Fe3Se4/PCM) is proposed to surmount these hurdles. The porous carbon skeleton offers a wealth of adsorption sites for the physical immobilization of polysulfides in tandem with excellent electrical conductivity. Beyond chemically fixing polysulfide intermediates, the embedded Fe3Se4 can facilitate the conversion reaction from Li2S2 to Li2S. Attributed to the superiority of Fe3Se4/PCM, the final batteries deliver a high utilization rate of sulfur (1179.2 mAh·g–1 at 0.1 C), a superb rate capability of 486.4 mAh·g–1 at 3 C, and prolonged cycling stability (a low degradation rate of 0.04% per cycle over 500 cycles at 1 C). This work may provide a novel formula for designing a string of transition-metal selenides/porous carbon composites as separator modifiers to achieve robust lithium–sulfur batteries.
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危险品化学品经营许可证（带存储）