CHINESE JOURNAL OF CATALYSIS
·
2025
·
IF 17.7
Pengkun Zhang,Qinhan Wu,Haoyu Wang,Dong-Hau Kuo,Yujie Lai,Dongfang Lu,Jiqing Li,Jinguo Lin,Zhanhui Yuan,Xiaoyun Chen
Herein, we established a Zn 3 (OH) 2 (V 2 O 7 )(H 2 O) 2 /V-Zn(O,S) Z-scheme heterojunction (labeled ZnVO/V-Zn(O,S) with a heterovalent V 4+ /V 5+ states and oxygen vacancies in both phases via a one-step in-situ hydrolysis method. The NaBH 4 regulated the ZnVO/V-Zn(O,S)-3 with rich Vo and suitable n (V 4+ )/ n (V 5+ ) ratio achieved an excellent photocatalytic nitrogen fixation activity of 301.7 μmol/(g·h) and apparent quantum efficiency of 1.148% at 420 nm without any sacrificial agent, which is 11 times than that of V-Zn(O,S). The Vo acts as the active site to trap and activate N 2 molecules and to trap and activate H 2 O to produce the H for N 2 molecules photocatalytic reduction. The rich Vo defects can also reduce the competitive adsorption of H 2 O and N 2 molecules on the surface active site of the catalyst. The heterovalent vanadium states act as the photogenerated electrons, quickly hopping between V 4+ and V 5+ to transfer for the photocatalytic N 2 reduction reaction. Additionally, the Z-scheme heterojunction effectively minimizes photogenerated carrier recombination. These synergistic effects collectively boost the photocatalytic nitrogen fixation activity. This study provides a practical method for designing Z-scheme heterojunctions for efficient photocatalytic N 2 fixation under mild conditions.
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高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2022
·
IF 6.633
Mansoor Khan,Ozgur Ozalp,Muslim Khan,Mustafa Soylak
In this work, we report the preparation of Fe 3 O 4 -Ti 3 AlC 2 max phase impregnated with 2-(5-Bromo-2-pyridylazo-5-(diethylamino) phenol) and its implementation for the extraction of cadmium, lead and cobalt from water and food samples. Blended Fe 3 O 4 -Ti 3 AlC 2 max phase impregnated with 2-(5-Bromo-2-pyridylazo-5-(diethylamino) phenol) was characterized using different instrumental techniques like scanning electron microscopy (SEM), surface area analyser (SAA), energy dispersive X-rays (EDX), Fourier transform infrared spectroscopy (FT-IR) and X-rays diffraction (XRD) for surface morphology, surface region, elemental composition, functional group and crystallinity, respectively. Factors that impact the % recoveries of analyte i.e pH, sample volume, vortex time, desorption condition and matrix impact were optimized. Analytical parameters like limit of detection (LOD) (Cd: 0.093 µg/L, Pb: 0.297 µg/L, Co: 0.223 µg/L), limit of quantifications (LOQ) (Cd: 0.310 µg/L, Pb: 0.992 µg/L, Co: 0.744 µg/L) preconcentration factors (PF) (Cd = Pb = Co: 40), enrichment factors (EF) (Cd: 39.8, Pb: 39, Co: 40) and relative standard deviation (RSD) (Cd: 3.8, Pb: 5.98, Co: 4.0) were determined under optimized trial conditions. The proposed strategy was tested using certified reference materials like TMDA-64.3 natural water and tobacco leaves (INCT-OBTL-5) for trace metal analysis. The developed strategy was also applied effectively to natural water samples and food samples using the standard addition method with good recovery results.
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ELECTROCHIMICA ACTA
·
2017
·
IF 4.798
Zhonghua Xue,Xiaoxia Fu,Honghong Rao,Xibin Zhou,Xiuhui Liu,Xiaoquan Lu
As efficient electron transfer mediator, various nanomaterials with smart performance have been explored in non-enzymatic sensor fabrication. We herein present an electron transfer mediator actuated electrochemical non-enzymatic sensor to assay nitrite, enabled by electrochemical reduced graphene oxide (ERGO) and 1-(2-pyridylazo)-2-naphthol (PAN). The composites of PAN-ERGO coating on electrode surface were successfully fabricated through a facile and green cyclic voltammetry technique. With the aid of ERGO nanosheets on the electrode surface, the electron transfer mediator of PAN nanostructures was easily immobilized and hybridized with ERGO, resulting in a remarkably synergistic effect between ERGO sensing substrate and the electrodeposited PAN coating for target electron transfer. The results demonstrated that the electrocatalytic current increased linearly with the nitrite concentration in the range of 7.49–1752.6 μM and 1752.6–3500 μM, with a good detection limit of 0.67 μM and 1.44 μM (signal-to-noise ratio, S/N = 3), respectively. The present work provide some new insights for the smart design of high efficiency electrocatalytic materials that based on the composites of electrochemical reduced graphene oxide and other substances.
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PROGRESS IN ORGANIC COATINGS
·
2021
·
IF 5.161
Muhammad Atif,Kang He,Muhammad Irfan,Yuchen Wang,Fei Hu,Lijuan Chen,Yanmei Wang
Protein adsorption at interfaces is a key for many applications especially in the biomedical field, biotechnologies, biocatalysis, food industry, etc. The development of surface layers that allow to control and manipulate protein adsorption is thus highly desired. To achieve this desired goal, we designed a smart thin stimuli responsive coating based on poly[(2-methyl-2-oxazoline)-random-glycidyl methacrylate)] (PMOXA-r-GMA) comb copolymer and poly(4-vinyl pyridine)-block-poly(glycidyl methacrylate) (P4VP-b-PGMA) block copolymer and scrutinized its ability to control protein adsorption properties by unraveling the effect of ionic strength (I) along with the pH of the surrounding medium and variation of chain length of P4VP. Firstly, PMOXA/P4VP based coatings were prepared by spin coating the mixture of PMOXA-r-GMA and P4VP-b-PGMA copolymer solutions onto silicon substrates followed by annealing at 110 °C. The surface composition and morphology of the PMOXA/P4VP based coatings were evaluated through X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The switchable ability of the coating with the variation of chain length of P4VP upon pH and ionic strength trigger was characterized by water contact angle (WCA) and variable angle spectroscopic ellipsometry (VASE). The experimental observations illustrated that the change in the water contact angle (ΔWCA) and thickness (Δh) were found to be maximum when the chain length of P4VP was almost three times that of PMOXA while turning the environmental condition from pH 3, with low ionic strength (I = 10 −3 M) to pH 9 with high ionic strength (I = 10 -1 M). Finally, the adsorption and desorption behavior of mixed polymer coating against bovine serum albumin (BSA) protein was analyzed qualitatively and quantitatively using the fluorescein isothiocyanate-labelled BSA (FITC-BSA) assay and quartz crystal microbalance with dissipation monitoring (QCM-D), respectively. The low ionic strength (I = 10 -3 M) at pH 3, caused to increase the adsorption of BSA and this increment was enhanced by increasing the chain length of P4VP in PMOX/P4VP based coatings. Subsequently, the adsorbed protein was then efficiently desorbed by changing the conditions to pH 9, I = 10 -1 M, as well as the maximum desorption percentage (> 92 %) could be obtained at these optimized conditions in PMOXA/P4VP based polymer coatings.
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Pharmaceuticals
·
2023
·Vol. 16
·期 5
·767
·
IF 4.6
Jie Chen,Xiang Zhou,Lifeng Fu,Haiyu Xu
Drugs that cure COVID-19 have been marketed; however, this disease continues to ravage the world without becoming extinct, and thus, drug discoveries are still relevant. Since Mprohas known advantages as a drug target, such as the conserved nature of the active site and the absence of homologous proteins in the body, it receives the attention of many researchers. Meanwhile, the role of traditional Chinese medicine (TCM) in the control of epidemics in China has also led to a focus on natural products, with the hope of finding some promising lead molecules through screening. In this study, we selected a commercial library of 2526 natural products from plants, animals and microorganisms with known biological activity for drug discovery, which had previously been reported for compound screening of the SARS CoV-2 S protein, but had not been tested on Mpro. This library contains compounds from a variety of Chinese herbs, includingLonicerae Japonicae Flos,Forsythiae FructusandScutellariae Radix, which are derived from traditional Chinese medicine prescriptions that have been shown to be effective against COVID-19. We used the conventional FRET method for the initial screening. After two rounds of selection, the remaining 86 compounds were divided into flavonoids, lipids, phenylpropanoids, phenols, quinones, alkaloids, terpenoids and steroids according to the skeleton structures, with inhibition rates greater than 70%. The top compounds in each group were selected to test the effective concentration ranges; the IC50values were as follows: (−)–gallocatechin gallate (1.522 ± 0.126 μM), ginkgolic acid C15:1 (9.352 ± 0.531 μM), hematoxylin (1.025 ± 0.042 μM), fraxetin (2.486 ± 0.178 μM), wedelolactone (1.003 ± 0.238 μM), hydroxytyrosol acetate (3.850 ± 0.576 μM), vanitiolide (2.837 ± 0.225 μM), β,β–dimethylacrylalkannin (2.731 ± 0.308 μM), melanin (7.373 ± 0.368 μM) and cholesteryl sodium sulfate (2.741 ± 0.234μM). In the next step, we employed two biophysical techniques, SPR and nanoDSF, to obtain KD/Kobsvalues: hematoxylin (0.7 μM), (−)–gallocatechin gallate (126 μM), ginkgolic acid C15:1 (227 μM), wedelolactone (0.9770 μM), β,β–dimethylacrylalkannin (1.9004 μM,), cholesteryl sodium sulfate (7.5950 μM) and melanin (11.5667 μM), which allowed better assessments of the binding levels. Here, seven compounds were the winners. Then, molecular docking experiments were specially performed by AutoDock Vina to analyze the mode of interactions within Mproand ligands. We finally formulated the present in silico study to predict pharmacokinetic parameters as well as drug-like properties, which is presumably the step that tells humans whether the compounds are drug-like or not. Moreover, hematoxylin, melanin, wedelolactone, β,β–dimethylacrylalkannin and cholesteryl sodium sulfate are in full compliance with the “Lipinski” principle and possess reasonable ADME/T properties, they have a greater potential of being lead compounds. The proposed five compounds are also the first to be found to have potential inhibitory effects on SARS CoV-2 Mpro. We hope that the results in this manuscript may serve as benchmarks for the above potentials.Keywords:SARS CoV-2 Mpro;natural products;high-throughput screening;lead compounds;in silico study
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PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2026
·
IF 8.2
Jie Qiao,Yibo Song,Nan Zhao,Peiyao Duan,Xiujuan Li
Acyltransferase MtMetA catalyzes the specific conversion of BHET to MHET. • Catalytic barrier-minimization strategy increased MHET yield by 3.13-fold. • The engineered mutants outperformed wild-type MtMetA in stability and activity. • Shortened nucleophilic attack distance governs the enhanced enzyme activity. The extensive use of polyethylene terephthalate (PET) has resulted in severe environmental pollution and ecological stress. Despite advances in PET recycling, current processes struggle to achieve high product value, as the complete conversion to terephthalic acid remains energetically demanding and economically inefficient. The catalytic promiscuity inherent in natural enzyme evolution holds great promise for providing novel candidates to accelerate PET biodegradation and upcycling. Herein, we report for the first time that the acyltransferase metA from Mycobacterium tuberculosis (MtMetA) catalyzes the conversion of bis(2-hydroxyethyl) terephthalate (BHET), an intermediate of PET hydrolysis, into the high-value monomer mono(2-hydroxyethyl) terephthalate (MHET). Guided by molecular dynamics (MD) simulations, we applied a catalytic barrier-minimization strategy to optimize the active-site environment of MtMetA, yielding engineered variants, notably ΔBarrier2 and ΔBarrier3. Specifically, ΔBarrier2 achieved a 3.1-fold increase in MHET yield, while ΔBarrier3 demonstrated a 3.2-fold enhancement in catalytic efficiency ( k cat / K M ) relative to the wild-type. The truncated MtMetA variants also exhibited enhanced robustness, showing improved thermostability (3.5-fold increase in residual activity at 60 °C for ΔBarrier3), as well as higher tolerance to metal ions and organic solvents. In particular, ΔBarrier2 displayed a 7.2-fold increase in product yield in Ca 2+ -containing systems, while ΔBarrier3 retained 2.3-fold higher residual activity in the presence of 50 % (v/v) isopropanol. MD simulations revealed that an enlarged active pocket and a shortened nucleophilic attack distance synergistically govern the enhanced catalytic activity and robustness. This work expands the enzymatic toolbox for PET recycling and targeted BHET degradation, advancing sustainable plastic waste management through biocatalytic innovations. Download: Download high-res image (113KB) Download: Download full-size image
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高影响因子
Processes
·
2026
·Vol. 14
·期 2
·242
·
IF 3.4
Yu Guo,Qisong Liu,Li Liang,Guihong Lan,Ming Duan
Hydrogen sulfide (H2S) prevalent in fuel gases such as natural gas and biogas necessitates removal prior to utilization or pipeline distribution. Biological desulfurization is considered a green purification technology employing sulfur-oxidizing bacteria (SOB) under ambient conditions to eliminate sulfur compounds, offering advantages including high efficiency, simplified equipment, and minimal chemical consumption. A highly efficient SOB TYWJ-2 was isolated in this study. Genomic analysis revealed that strain TYWJ-2 possesses a complete set of sulfur metabolism genes, enabling the metabolism of various inorganic sulfides, along with salt-tolerance genes that support adaptation to high osmolarity environments. The optimal conditions for desulfurization were determined through single-factor experiments and Box–Behnken response surface methodology. Long-term desulfurization performance demonstrated stable operational efficiency, with H2S removal rates consistently reaching 99.72~99.87%. System performance remained robust under varying sulfur loads, elevated salinity, and intermittent operational shutdowns, with no significant decline in desulfurization efficiency observed. These findings indicate that strain TYWJ-2 holds considerable potential for the biological desulfurization of sulfur-containing biogas and natural gas.
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Advanced Healthcare Materials
·
2026
·
IF 11
Minjiang Chen,Kun Zhang,Kai Li,Hui Xu,Jiayi Shi,Huilin Hu,Qianhui He,Liyun Zheng,Shiji Fang,Qiaoyou Weng,Min Xu,Zhongwei Zhao,Gaofeng Shu,Jiansong Ji
The acidic and immunosuppressive tumor microenvironment (TME) remarkably hampers the therapeutic efficacy of transcatheter arterial embolization (TAE) for hepatocellular carcinoma (HCC). Herein, uniform CaCO 3 nanoparticles encapsulated nickel–alginate microspheres (CaNPs/Ni-Alg MSs) were engineered using a microfluidic device, serving as an immune-regulated embolic agent to amplify TAE treatment of HCC. CaNPs/Ni-Alg MSs could adsorb His-tagged interleukin-2 (IL-2) and enable sustained release through coordination interactions between the His-tag and Ni 2 + , thereby reducing systemic cytokine elevation risk from IL-2 administration. IL-2-loaded microspheres (IL-2/CaNPs/Ni-Alg MSs) effectively neutralize intratumoral lactic acid (LA) via CaCO 3 , modulating the acidic TME. Proton neutralization restores IL-2 activity, enhancing T lymphocyte infiltration and proliferation. Additionally, lactate made available following acid neutralization may serve as a metabolic substrate, as supported by the calcium lactate and neutralization-mimicking experiments in vitro, contributing to CD8 + T-cell metabolic fitness. IL-2/CaNPs/Ni-Alg MSs could reprogram the TME into an immune-promoting milieu, synergizing with immune checkpoint blockade (ICB) therapy to suppress both primary and distant tumors. Not surprisingly, TAE with IL-2/CaNPs/Ni-Alg MSs combined with αPD-L1 achieved superior therapeutic efficacy without side effect in a rat orthotopic HCC model. Overall, this work presents a safe metabolism-reprogramming embolic strategy that repurposes LA to boost IL-2–mediated embolization-immunotherapy in HCC.
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高影响因子
Journal of Materials Chemistry A
·
2026
·
IF 9.2
Chao Yang,Xiao-Li Pu,Qi Yin,Qing-Ping Huang,Lei Cai,Ping Liu,Zhi-Bin Fang
Elucidating properties of cocatalysts from all aspects endows them new roles as proactive regulators of light harvesting and reaction kinetics to promote the overall efficiency of photocatalysts. [Mo3S13]2- nanoclusters have served as one of the most efficient catalysts for hydrogen evolution reaction (HER), while their underlying photo-responsive behaviors within hybrid photocatalyst systems remain unexplored. Herein, we unveil the visible-light sensitization effect of [Mo3S13]2- nanoclusters, in the mode of hot electron injection, that drive apparent and sustainable photocatalytic HER. This effect was demonstrated by loading [Mo3S13]2- nanoclusters on wide-bandgap nanosized ZnS host. Detailed characterizations further clarified the photosensitization of [Mo3S13]2- nanoclusters by revealing the dependence of HER activity on the enhanced visible light responsivity and the light-induced nanocluster-to-host electron transfer. This work not only showcases the dual function of the advanced [Mo3S13]2- nanocluster cocatalysts but also provides guidance for the discovery and understanding of more multifunctional materials opening new opportunities for efficient and multi-mode photocatalysis.
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ACS Nano
·
2026
·
IF 17.3
Ping Li,Qianying Li,Lu Ding,Shiyu Peng,Hui Wei,Tingtao Chen,Yonghua Xiong,Ben Zhong Tang,Xiaolin Huang
Interleukin-2 (IL-2) immunotherapy offers considerable potential for metastatic cancers; however, its efficacy is limited by low response rates, dose-dependent toxicity, short half-life, poor tumor accumulation, and off-target immune activation. In this work, we present a biohybrid microrobot (IL-2@Z/C/A) engineered through biomimetic mineralization of IL-2 variant-secreting Escherichia coli Nissle 1917 (EcN) with a zeolitic imidazolate framework-8 (ZIF-8) shell coloaded with an aggregation-induced emission photosensitizer and catalase. The ZIF-8 coating preserves bacterial viability tumor-homing capability while preventing premature drug leakage and systemic exposure, thus minimizing off-target effects. Upon accumulation in the acidic tumor microenvironment (TME), the framework degrades to release engineered bacteria and therapeutic cargo. Locally delivered zinc ion release, light-triggered reactive oxygen species and EcN derived pathogen-associated molecular patterns act synergistically to induce Cle-caspase/GSDMD mediated pyroptosis, resulting in immunogenic cell death accompanied by damage-associated molecular pattern release and pro-inflammatory cytokine production. Simultaneously, catalase-driven oxygen generation alleviates hypoxia and suppresses HIF-1α-induced immunosuppression. In combination with PD-L1 blockade, IL-2@Z/C/A achieves near-complete tumor regression in a B16F10 melanoma model via a coordinated immune cascade: pyroptosis-mediated antigen exposure primes adaptive immunity, hypoxia reversal counteracts immunosuppression, and sustained local IL-2 release reverses T cell exhaustion, collectively reprogramming the immunosuppressive TME and eliciting strong antitumor immunity. This work establishes a distinct paradigm for spatially controlled immunotherapy and highlights the potential of this biohybrid microrobot in converting immunologically “cold” tumors into responsive niches.
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高影响因子
Journal of Environmental Sciences
·
2016
·
IF 2.208
Jingjing Shao,Yan Cheng,Chunping Yang,Guangming Zeng,Wencan Liu,Panpan Jiao,Huijun He
Naphthalene-2-ol is a typical biologically recalcitrant pollutant in dye wastewater. Solvent extraction of naphthalene-2-ol from aqueous solutions using mixed solvents was investigated. Various extractants and diluents were evaluated, and the effects of volume ratio of extractant to diluent, initial pH, initial concentration of naphthalene-2-ol in aqueous solution, extraction time, temperature, volume ratio of organic phase to aqueous phase (O/A), stirring rate and extraction stages, on extraction efficiency were examined separately. Regeneration and reuse of the spent extractant were also investigated. Results showed that tributyl phosphate (TBP) achieved 98% extraction efficiency for naphthalene-2-ol in a single stage extraction, the highest among the 12 extractants evaluated. Extraction efficiency was optimized when cyclohexane and n -octane were used as diluents. The solvent combination of 20% TBP, 20% n -octanol and 60% cyclohexane ( V / V ) obtained the maximum extraction efficiency for naphthalene-2-ol, 99.3%, within 20 min using three cross-current extraction stages under the following extraction conditions: O/A ratio of 1:1, initial pH of 3, 25°C and stirring rate of 150 r/min. Recovery of mixed solvents was achieved by using 15% ( W / W ) NaOH solution at an O:A ratio of 1:1 and a contact time of 15 min. The mixed solvents achieved an extraction capacity for naphthalene-2-ol stably higher than 90% during five cycles after regeneration.
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高影响因子
JOURNAL OF SOLID STATE CHEMISTRY
·
2015
·
IF 2.133
Xiaoxiang Xu,Yinghao Xie,Shuang Ni,Abul K. Azad,Tongcheng Cao
Solid solutions of ZnGa 2− x Cr x O 4 (0≤ x ≤2) were successfully prepared by solid state reactions . Introducing Cr into ZnGa 2 O 4 crystal structure significantly improves its light absorption and greatly enhances its photocatalytic activity . An optimized Cr content ( x =1.5 for ZnGa 0.5 Cr 1.5 O 4 ) was found for the solid solutions with the highest photocatalytic hydrogen production rate (~775 μmol/h). Compositional analysis suggests there is a strong enrichment of Cr at the surface of sample ZnGa 0.5 Cr 1.5 O 4 compared with other samples. Theoretical calculations suggest their electronic structures involve spin-polarized conduction band (CB) and valence band (VB) that are mainly composed of Cr 3 d orbitals. The highest photocatalytic activity observed in ZnGa 0.5 Cr 1.5 O 4 is probably due to the higher Cr content at the surface that favors the Cr 3 d orbital overlapping.
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