ACS Omega
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2025
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IF 4.3
Zhenghu Min,Xingdan Wang,Xiaoqiu Yang,Qiwei Zhang,Qi Zheng
Glycosylation profiling is an effective methodology for achieving a comprehensive understanding of glycoproteins and their alterations in a multitude of pathological conditions. However, in comparison to N-glycosylation, O-glycosylation presents significant challenges in terms of both qualitative and quantitative mass spectrometric analyses. A recently developed oxidative release protocol enables the selective formation of O-glycans containing a carboxyl group derived from the amino acid residue. In this study, 3-nitrophenylhydrazine was used to derivatize the common carboxyl group in a mild hydrophilic solution. Derivatization resulted in the generation of a series of report ions for serine, threonine, sialic acid, and O-acetylated sialic acid residues, thereby facilitating the identification of O-glycans and their attached amino acid residues, as well as the determination of the number of O-acetyl groups. A total of 65 O-glycans can be identified from bovine mucin. Furthermore, the analytical strategy revealed that O-acetylated N-acetylneuraminic acid (Neu5Ac)-containing O-glycans from horse serum exhibited distinctive fragmentation patterns in comparison to those from bovine mucin. Additionally, the presence of deaminoneuraminic acid (KDN)-containing O-glycans was successfully confirmed in fish intestinal tissue. These findings suggest that this method provides an economical and potentially valuable tool for large-scale O-glycosylation studies in complex biological samples.
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BIOTECHNOLOGY LETTERS
·
2019
·Vol. 41
·期 6
·789-799
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IF 2.154
Wang Xiuwen,Cheng Yang,Jin Qingzhe,Wei Wei,Wang Xingguo
Objectives To investigate the lipase-catalyzed synthesis of high purity sn -1/3 and sn -2 monoacylglycerols (1/3-MAG and 2-MAG) of different fatty acids (FAs). Results The 1/3-MAGs of three FAs (16:0, 17:0, 16:1) were synthesized using lipase-catalyzed esterification of glycerol with FAs. The 2-MAGs were obtained from the ethanolysis of synthetic triacylglycerols using sn -1,3 regiospecific lipase. The effects of lipase types, substrate ratio, temperature, reaction time and lipase load on the MAG conversion were studied. Under the optimal conditions, high purities (96.74%, 95.44%, 92.96%) with acceptable isolated yields (51.00%, 54.28%, 46.00%) were obtained for 1/3-16:0-MAG, 1/3-17:0-MAG, and 1/3-16:1-MAG, respectively. For 2-16:0-MAG, 2-17:0-MAG, and 2-16:1-MAG, the purities were 92.64, 95.04, and 96.48%, with isolated yields of 50.64, 52.16, and 26.12%, respectively. The molecular structures of the synthetic compounds were confirmed by 1 H NMR, and MS and the melting points were characterized by DSC. Conclusions High purity MAG isomers can be synthesized via lipase-catalyzed reactions to be building blocks for production of functional lipids, the melting points of which are largely governed by the hydrophobic interactions among the alkanyl chains.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2015
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IF 2.912
Chang Li,Hanbing Jia,Mingyue Shen,Yuting Wang,Shaoping Nie,Yi Chen,Yongqiang Zhou,Yuanxing Wang,Mingyong Xie
The capacities of six antioxidants to inhibit the formation of 3-monochloropropane-1,2 diol (3-MCPD) esters were examined in this study. Inhibitory capacities of the antioxidants were investigated both in chemical models containing the precursors (tripalmitoyl glycerol, 1,2-dipalmitoyl-sn-glycerol, monopalmitoyl glycerol, and sodium chloride) of 3-MCPD esters and in oil models (rapeseed oil and sodium chloride). Six antioxidants, butylated hydroxytoluene (BHT), butylated hydroxy anisole (BHA), tert-butyl hydroquinone (TBHQ), propyl gallate (PG), l-ascorbyl palmitate (AP), and α-tocopherol (VE), were found to exhibit inhibiting capacities on 3-MCPD ester formation both in chemical models and in oil models. TBHQ provided the highest inhibitory capacity both in chemical models and in oil models; 44% of 3-MCPD ester formation was inhibited in the presence of TBHQ (66 mg/kg of oil) after heating of rapeseed oil at 230 °C for 30 min, followed by PG and AP. BHT, BHA, and VE appeared to have weaker inhibitory abilities in both models. VE exhibited the lowest inhibition rate; 22% of 3-MCPD esters were inhibited in the presence of VE (172 mg/kg of oil) after heating of rapeseed oil at 230 °C for 30 min. In addition, the inhibition rates of PG and VE decreased dramatically with an increase in temperature or heating time. The results suggested that some antioxidants, such as TBHQ, PG, and AP, could be the potential inhibitors of 3-MCPD esters in practice.
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EUROPEAN JOURNAL OF INORGANIC CHEMISTRY
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2025
·
IF 2
Yue-Ling Sun,An-Hua Gong
A new barium zincophosphite BaZn 2 (HPO 3 ) 3 is obtained via a simple hydrothermal reaction. BaZn 2 (HPO 3 ) 3 crystallizes in the P space group and exhibits a novel 3D crystal structure. It possesses a short ultraviolet cutoff edge of 205 nm, corresponding to a large experimental bandgap of 5.55 eV. BaZn 2 (HPO 3 ) 3 also has good thermal stability. This present work provides a comprehensive characterization of its crystal structure and optical properties. Additionally, first-principles calculations are employed to analyze the structure–activity relationship of BaZn 2 (HPO 3 ) 3 . Graphical BaZn 2 (HPO 3 ) 3 possesses a novel 3D anion framework, short ultraviolet cutoff edge, large bandgap, and good thermal stability.
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RSC Advances
·
2021
·Vol. 12
·期 1
·595-601
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IF 3.361
Lijuan Chen,Xiang Li,Zezhi Li,Kejian Liu,Jianping Xie
Peroxidases are present widely in microorganisms and plants, and catalyze many reactions. However, the activity of natural peroxidases is susceptible to external conditions. We prepared carbon nanoparticles (CNPs) using an environmentally friendly and simple method. These CNPs were demonstrated to possess intrinsic peroxidase-like activity. CNPs could catalyze the reaction of a peroxidase substrate, 3,3,5,5-tetramethylbenzidine (TMB), in the presence of H2O2 to produce a blue solution at 652 nm. CNPs exhibited higher peroxidase activity than that of other carbon-based nanomaterials. Moreover, CNPs retained their high peroxidase activity after being reused several times. Glutathione (GSH) can change the blue color of oxidized TMB into a colorless hue at 652 nm. Based on this fact, qualitative and quantitative approaches were employed to detect GSH using a colorimetric method. This method showed a broad detection range (2.5–50 μM) with a limit of detection of 0.26 μM. This method was shown to be accurate for GSH detection in a cell culture medium compared with that using a commercial assay kit. Our findings could facilitate application of CNPs in biomedical areas.
研究领域:
纳米技术
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生物化学
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分析化学
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生物医学工程
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DRUG DEVELOPMENT RESEARCH
·
2019
·Vol. 80
·期 6
·750-757
·
IF 1.742
Chang Zheng,Wen Hou,Jun Liu,Xiao-Fang Xu,Jing Lin,Ping-Hua Sun,Wei-Min Chen
The prevalence of multidrug resistance among clinically significant bacterial pathogens underlines a critical need for the development of new classes of antibacterial agents with novel structural scaffolds. Cajaninstilbene acid (CSA), which is isolated from pigeonpea leaves, has shown potent antibacterial activity. In this study, a series of 2-hydroxyl-4-methoxyl-3-(3-methylbut-2-en-1-yl)-6-(4-phenylbenzoylamino)benzoic acid derivatives derived from CSA were designed and synthesized, and their antibacterial activities were evaluated. Several synthesized compounds exhibit better antibacterial activity than CSA against Staphylococcus aureus , Staphylococcus epidermidis , and two strains of methicillin-resistant S. aureus . Meanwhile, the results of 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide assays illustrate the good selectivity between bacteria and normal cells of the most active compounds 6u and 6v . Furthermore, well combinations with bacterial RNA polymerase of 6u arising from docking study imply the possible mechanism of antibacterial activity of these synthetic compounds.
研究领域:
药物化学
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微生物学
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合成化学
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MOLECULAR NUTRITION & FOOD RESEARCH
·
2025
·Vol. 70
·期 1
·e70363
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IF 4.2
Shaoxuan Wang,Fei Pan,Xinyan Xu,Qian Fan,Yangyang Wang,Lei Zhao,Liang Zhao
Heterocyclic aromatic amines (HAAs), potential carcinogens formed in processed meats, have been linked to liver injury. This study evaluated whether cyanidin-3-O-glucoside (C3G, 100 mg/kg) protects against liver injury by the representative HAA 2-amino-3-methylimidazo[4,5-f]quinoline (IQ, 20 mg/kg) in mice, and explored mechanisms by integrated approaches: biochemical assays, histology, Western blot, and molecular dynamics (MD) simulations. Results demonstrated that C3G significantly ameliorated IQ-induced liver injury, as indicated by reduced serum AST and ALT and inflammatory cytokines (IL-6, IL-1β, TNF-α) levels. Additionally, C3G restored redox homeostasis by inhibiting lipid peroxidation and protein carbonylation, while enhancing antioxidant defenses (GSH, GSH-Px, and SOD). Mechanistically, C3G exerted its protective effects via multiple anti-apoptotic pathways. Specifically, it attenuated mitochondrial apoptosis by lowering the Bax/Bcl-2 ratio and inhibiting cytochrome c-caspase-9/3 activation. It also alleviated endoplasmic reticulum (ER) stress by downregulating GRP78/ATF4/CHOP and caspase-12. Furthermore, C3G interfered with death receptor signaling by reducing Fas/FasL/FADD activation and caspase-8, while upregulating CFLAR. MD simulations further demonstrated that C3G binds to the hydrophobic pocket of BAX and weakens BAX-BIM interactions. These results conclusively identify C3G as a multi-target agent against IQ-induced liver damage, highlighting its promise for development into a novel dietary strategy to counteract the toxicological risks of processed meat consumption.
研究领域:
毒理学
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分子生物学
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营养生物化学
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药理学
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计算生物学
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食品科学
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JOURNAL OF ORGANIC CHEMISTRY
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2019
·
IF 4.745
Yong-Long Zhao,Xing-Hai Fei,Yong-Qin Tang,Peng-Fei Xu,Fen-Fen Yang,Bin He,Xiao-Zhong Fu,Yuan-Yong Yang,Meng Zhou,Yuan-Hu Mao,Yong-Xi Dong,Chun Li
An organocatalytic asymmetric α-sulfenylation of 2-substituted indolin-3-ones with N-(alkylthio or arylthio)succinimides has been developed for the first time using Cinchona-derived squaramide as the catalyst. Various chiral 2,2-disubstituted indole-3-ones with S- and N-containing heteroquaternary carbon stereocenters were obtained with up to 98% yield and 99% ee.
研究领域:
有机化学
·
合成化学
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JOURNAL OF CHROMATOGRAPHY A
·
2022
·
IF 4.601
You-Ping Zhang,Ling-Xiao Xiong,Ying Wang,Kuan Li,Bang-Jin Wang,Sheng-Ming Xie,Jun-Hui Zhang,Li-Ming Yuan
Polyimine macrocycles are a new class of organic macrocycles with cyclic structures, well-defined molecular cavities, and multiple cooperative binding sites, which have recently aroused considerable research interest in molecular recognition and separation. Herein, we report the bonding of a [3+3] chiral polyimine macrocycle (H 3 L, C 78 H 78 N 6 O 3 ) on thiol-functionalized silica gel using thiol-ene click chemistry to prepare a chiral stationary phase (CSP) for high performance liquid chromatography (HPLC). The fabricated column exhibited excellent chiral separation capability under both normal-phase and reversed-phase conditions. Fourteen and 10 racemates were well resolved on the column in normal-phase mode (using n -hexane/isopropanol as the mobile phase) and reversed-phase mode (using methanol/water as the mobile phase), respectively, including alcohols, esters, ethers, ketones, aldehydes, epoxides and organic acids. Moreover, the column also shows good selectivity toward positional isomers. Six positional isomers (dinitrobenzene, chloroaniline, bromoaniline, iodoaniline, nitrobrobenzene and nitrochlorobenzene) were well separated on the column. In addition, the effects of the injection mass and mobile phase composition on the separation were investigated. The column shows good reproducibility and stability after multiple injections with the relative standard deviation (RSD) ( n = 5) of the retention time and resolution being < 0.96 % and 0.65 %, respectively. This study indicates that this type of chiral polyimine macrocycles is a promising chiral selector for HPLC enantioseparation and will push forward the applications of more novel chiral macrocycles for chiral chromatographic separation.
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EUROPEAN JOURNAL OF PHARMACOLOGY
·
2019
·
IF 3.17
Qi-meng Liu,Qing Xiao,Xiang Zhu,Kai-feng Chen,Xia-wen Liu,Ru-chao Jiang,Dan Wu,Jun-min Shi,Li-jun Dai,Jun-jun Huang
Benign prostatic hyperplasia (BPH) is a common disorder of the urinary system in aging men. 2-(1H-indol-3-yl)-N-[3-(4-(2-methoxyphenyl) piperazinyl) propyl] acetamide (HJZ-3), which is derived from naftopidil , exhibited 97.7- and 64.6-fold greater inhibitory effects for a 1D adrenoceptor than for a 1B - and a 1A -adrenoceptors in vitro, respectively. To investigate the therapeutic potential for treating BPH, we evaluated the pharmacological activity of HJZ-3. Specifically, we evaluated through estrogen/androgen-induced rat benign prostatic hyperplasia model in vivo. HJZ-3 effectively prevented the progression of rat prostatic hyperplasia by suppressing the increase in prostate index and reducing the quantitative analysis of the relative acinus volume, relative stroma, epithelial volume and epithelial thickness and expression of proliferating cell nuclear antigen and α -smooth muscle actin. HJZ-3 decreased α 1A - and α 1D -adrenoceptor protein expressions in prostate tissue. HJZ-3 is a good alternative for α 1A - and α 1D -adrenoceptor blocker. It may relax smooth muscle tone and relieve symptoms of BPH.
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ChemPlusChem
·
2020
·Vol. 85
·期 1
·240-246
·
IF 2.753
Sheng Tang,Xinyu Xiao,Jing Hu,Bo Gao,Hunglin Chen,Zongyang Peng,Jianchun Wen,Masanao Era,Dechun Zou
Graphical Solvent-free is best : Mixed halide hybrid perovskites (MHPs) are prepared through a solvent-free mechanochemical synthesis (MCS) route with superior control over halide compositions. The MCS route eliminates the problem in the preparation of MAPb(I x Br 1− x ) 3 with continuously varying x , while maintaining the material properties and suppressing phase segregation in solvent synthesis (SS) routes. Thus, the optical band gaps can be tuned easily by compositional control. Hybrid perovskites have recently received much attention in optoelectronic applications. However, hybrid perovskites are unstable in a humid environment. Mixed halide perovskites (MHPs) show enhanced stability and band-gap tunability upon engineering of their halide composition. Here, MHPs are prepared through a solvent-free mechanochemical synthesis (MCS) route that allows superior control over halide compositions than the solvent synthesis routes (SS). The MCS route eliminates the problem in the preparation of MAPb(I x Br 1− x ) 3 with continuously varying x , while maintaining the material properties and suppressing phase segregation present in SS routes. UV-vis absorption and X-ray diffraction patterns confirm the production of the desired pure-phase MHPs. For MAPb(I x Br 1− x ) 3 (0≤ x ≤1), with increased ratio of halide ( x ), the cubic phase gradually transforms into the tetragonal phase and band-gap tunability is accomplished. The MCS route for the preparation of MHPs is a very promising and efficient technique for superior control in optoelectronic properties, leading to improved control in fabrication approaches.
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International Journal of Nanomedicine
·
2019
·
IF 4.471
Dian Chen,Tonghe Zhu,Wei Fu,Haibo Zhang
Purpose A promising vascular scaffold must possess satisfying mechanical properties, great hemocompatibility, and favorable tissue regeneration. Combining natural with synthetic materials is a popular method of creating/enhancing such scaffolds. However, the effect of additional modification on the materials requires further exploration. Materials and methods We selected polycaprolactone (PCL), which has excellent mechanical properties and biocompatibility and can be combined with collagen. Electrospun fibers created using a PCL/collagen solution were used to fashion mixed nanofibers, while separate syringes of PCL and collagen were used to create separated nanofibers, resulting in different pore sizes. Mixed and separated nanofibers were cross-linked with glutaraldehyde (GA), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), and genipin; hence, we named them as mixed GA, mixed EDC (ME), mixed genipin (MG), separated GA, separated EDC (SE), and separated genipin (SG). Results Fourier transform infrared (FTIR) spectroscopy and X-ray diffraction showed that cross-linking did not affect the main functional groups of fibers in all groups. ME, MG, SE, and SG met the requisite mechanical properties, and they also resisted collagenase degradation. In hemocompatibility assays, only ME and MG demonstrated ideal safety. Furthermore, ME and MG presented the greatest cytocompatibility. For vascular scaffolds, rapid endothelialization helps to prevent thrombosis. According to human umbilical vein endothelial cell migration on different nanofibers, ME and MG are also successful in promoting cell migration. Conclusion ME and MG may be promising candidates for vascular tissue engineering. The study suggests that collagen cross-linked by EDC/ N -hydroxysuccinimide or genipin facilitates endothelial cell regeneration, which could be of great benefit in tissue engineering of vascular scaffolds.
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