Natural Product Communications
·
2025
·
IF 1.4
Zhuowei Shen,Yanhong Chen,Kui Zeng,Haihong Hu,Jie Pan,Mingcheng Xu,Kaifeng He,Fengting Ou,Lushan Yu,Su Zeng,Lu Chen
Background6-hydroxykynurenic acid (6-HKA), isolated from Ginkgo biloba leaves, has demonstrated neurorestorative properties against cerebral ischemia in middle cerebral artery occlusion (MCAO) mice models. However, our previous studies showed that 6-HKA was difficult to penetrate the blood brain barrier (BBB) to exert its pharmacological effects in rats. Therefore, this study aims to investigate the pharmacokinetics and cerebral cortex distribution of 6-HKA in MCAO mice, to determine whether the ability of 6-HKA to cross the BBB is contingent upon barrier integrity.MethodsThis study established and validated the ultra-performance liquid chromatography tandem mass spectrometric (UPLC-MS/MS) methods for the quantification of 6-HKA in mouse plasma and brain, and investigated the pharmacokinetics and cerebral cortex distribution of 6-HKA in MCAO mice and sham-operated mice.ResultsThis study demonstrates that the mean residence time (MRT0−t) of 6-HKA in the MCAO group was significantly prolonged compared to the sham-operated group, and there was a higher distribution of 6-HKA in the ischemic cerebral cortex than in the contralateral normal cerebral cortex.ConclusionThis study illustrated that in MCAO mice, the prolonged MRT0−t of 6-HKA and the increased permeability of BBB made the accumulation of 6-HKA in the ischemic cerebral cortex, thereby enabling 6-HKA to exert neurorestorative effects.
✓ 已验证
DOI
高影响因子
PLANT CELL REPORTS
·
2019
·Vol. 39
·期 1
·63-73
·
IF 3.499
Wang Xiaomin,Ruan Mengjiao,Wan Qi,He Wenliang,Yang Lei,Liu Xinyuan,He Li,Yan Lili,Bi Yurong
Key message Changes in glucose-6-phosphate dehydrogenase (G6PD) isoforms activities and expression were investigated in soybean roots under drought, suggesting that cytosolic G6PD plays a main role by regulating H 2 O 2 signal and redox homeostasis. G6PD acts a vital role in plant growth, development and stress adaptation. Drought (PEG6000 treatment) could markedly increase the enzymatic activities of cytosolic G6PD ( Cyt-G6PD ) and compartmented G6PD (mainly plastidic P2-G6PD) in soybean roots. Application of G6PD inhibitor upon drought condition dramatically decreased the intracellular NADPH and reduced glutathione levels in soybean roots. Nitric oxide (NO) and hydrogen peroxide (H 2 O 2 ) participated in the regulation of Cyt-G6PD and P2-G6PD enzymatic activities under drought stress. Diphenylene iodonium (DPI), an inhibitor of NADPH oxidase, abolished the drought-induced accumulation of H 2 O 2 . The exogenous application of H 2 O 2 and its production inhibitor (DPI) could stimulate and inhibit the NO accumulation, respectively, but not vice versa. qRT-PCR analysis confirmed that NO, as the downstream signal of H 2 O 2 , positively regulated the transcription of genes encoding Cyt-G6PD ( GPD5 , G6PD6 , G6PD7 ) under drought stress in soybean roots. Comparatively, NO and H 2 O 2 signals negatively regulated the gene expression of compartmented G6PD ( GPD1 , G6PD2 , G6PD4 ), indicating that a post-transcriptional mechanism was involved in compartmented G6PD regulation. Taken together, the high Cyt-G6PD activity is essential for maintaining redox homeostasis upon drought condition in soybean roots, and the H 2 O 2 -dependent NO cascade signal is differently involved in Cyt-G6PD and compartmented G6PD regulation.
✓ 已验证
DOI
高影响因子
CHINESE JOURNAL OF POLYMER SCIENCE
·
2026
·
IF 4
Wu Jia-Le,Xiao Wen-Hao,Wu Jing-Bo,Lin Jiang-Hua,Xu Rui-Jie,Lei Cai-Hong
The chemical structure of polyamide 6 (PA6) dictates that only 50% of hydrogen bonds participate in crystallization during the crystallization process, resulting in the properties of its products being significantly dependent on the molding process. Therefore, the design and development of nucleating agents suitable for PA6 holds great practical significance for high-performance PA6 materials. Amide-based nucleating agents can effectively improve the crystallization rate by increasing intermolecular hydrogen bond density. Further introduction of hydroxyl groups can enhance the hydrogen bonding interactions between the nucleating agent and PA6. In this study, a hydroxyl-containing amide-based nucleating agent, BHT, was designed and synthesized using a tyramine-based biomass as the raw material. These results demonstrated that BHT exhibited good structural compatibility with PA6. After adding 1 wt% BHT, the crystallization temperature of PA6 increased from 170.9 °C to 193.3 °C, the crystallinity increased 16.6%, the heat distortion temperature and Vicat softening temperature rose to 89.5 and 187.8 °C, respectively, the haze decreased to 46%, achieving the synergistic optimization of mechanical, thermal, and optical properties. The in situ time-resolved FTIR results indicated that the addition of BHT increased the enthalpy of hydrogen bond formation during the nucleation stage, facilitated the segmental conformation adjustment of PA6, and enhanced the molar concentration of trans-conformations, ultimately leading to an improvement in the crystallization rate.
✓ 已验证
产品关联
DOI
高影响因子
Journal of Physical Chemistry C
·
2017
·
IF 4.536
Wanfeng Li,Fang Zhang,Xingde Xiang,Xiucheng Zhang
Nickel-based ferrocyanides have attracted wide interest as cathode for aqueous sodium-ion batteries in fields of sustainable energies such as wind and solar, owing to excellent cycling stability and high-rate capability. However, they suffer from low specific capacities (∼60 mAh g–1). Herein, Na2Ni0.4Co0.6[Fe(CN)6] nanocrystallites are reported for the first time as high-capacity cathode for aqueous sodium-ion batteries. Its electrochemical properties and redox mechanism have been understood by combining the X-ray diffraction technique, Fourier-transform infrared spectroscopy, cyclic voltammetry, electrochemical impedance microscopy, and charge/discharge measurements. It is revealed that the material undergoes a reversible three-step single-phase reaction mechanism during Na extraction through sequential electrochemical oxidation of nitrogen-coordinated Co2+ ions and carbon-coordinated Fe2+ ions and achieves superior electrochemical performance with a high reversible capacity of 85 mAh g–1 at 0.5 C, an average operating potential of 0.62 V (vs Ag/AgCl), and a high capacity retention of 90% after 100 cycles. The combination of high specific energy and good cycling performance enables the Na2Ni0.4Co0.6[Fe(CN)6] material exhibiting promising application for high-performance aqueous sodium-ion batteries.
研究领域:
电化学
·
储能
·
材料科学
·
电池技术
✓ 已验证
DOI
高影响因子
EUROPEAN JOURNAL OF PHARMACOLOGY
·
2026
·
IF 4.7
Wenying Fu,Ying Yan,Chongchong Shu,Chenxin Xu,Yinghua Chen,Yiqun Xia,Jundixia Chen,Yunzhi Chen,Ri Cui,Peng Zou,Daoyong Ni
6-Methoxydihydrosanguinarine is a natural alkaloid derived from medicinal plants that exhibits significant antitumor activity, making it a promising candidate for cancer therapy. However, the exact molecular mechanisms underlying its effects require further investigation. In this study, we investigated the cytotoxicity and underlying mechanisms of 6-Methoxydihydrosanguinarine in human non-small cell lung cancer (NSCLC) cells. Our findings reveal that reactive oxygen species (ROS) accumulation is the key driver of its antitumor activity. Mechanistically, 6-Methoxydihydrosanguinarine activates the JNK signaling pathway and induces endoplasmic reticulum (ER) stress, both of which can be reversed by the ROS scavenger N-acetylcysteine (NAC). Interestingly, 6-Methoxydihydrosanguinarine also activates autophagy, and inhibition of autophagy reverses the JNK and ER stress pathway activation induced by 6-Methoxydihydrosanguinarine. Notably, 6-Methoxydihydrosanguinarine synergistically enhances cisplatin-induced NSCLC cell death, and this synergistic effect is abolished by NAC, highlighting the critical role of ROS accumulation in their combined efficacy. This study systematically elucidates the molecular mechanisms of 6-Methoxydihydrosanguinarine against NSCLC, revealing that, in addition to the JNK and autophagy pathways, ER stress also mediates its antitumor effects. Moreover, our data establish a rationale for exploring 6-Methoxydihydrosanguinarine in NSCLC therapy and highlight its combination with cisplatin as a potentially effective strategy.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POLYMER ENGINEERING AND SCIENCE
·
2026
·
IF 3.6
Wenqiao Meng,Kun Zhang,Wenxing Chen,Shichang Chen
Improving the toughness and reducing the oligomer content of Polyamide 6 through a green and sustainable bio-based modification strategy is of great significance for expanding its application potential. In this study, bio-based itaconic acid pentanediamine salt (IPA) was copolymerized with ε-caprolactam to prepare a bio-based copolyamide (PA6-IPA) containing pyrrolidone rings. The regulatory effects of IPA content on the oligomer content, thermal behavior, rheological properties, and mechanical properties of PA6-IPA were systematically investigated. The successful synthesis of PA6-IPA was confirmed by 1 H NMR and FT-IR. The results showed that with the increase of IPA content, the oligomer content of PA6-IPA decreased from 8.68% to 2.76%, the crystallinity crystallization temperature decreased significantly, and the relative content of crystal forms was regulated. In addition, PA6-IPA 20 achieved an elongation at break of 382.5% and a tensile strength of 58 MPa, realizing excellent toughness enhancement while maintaining the original strength. This can be attributed to the addition of IPA, which disrupts the regularity of the chain segments, increases the amorphous region of the copolyamide, and enhances the mobility of the molecular chains. This study provides a feasible strategy for developing eco-friendly, high-performance bio-based polyamides with balanced processability and mechanical properties. Highlights Bio-based PA6 copolymerized with itaconic acid-pentanediamine salt. The addition of IPA salt reduced the oligomers content from 8.68% to 2.76%. PA6-IPA 20 exhibited 382.5% elongation and 58 MPa strength.
✓ 已验证
DOI
高影响因子
NATURAL PRODUCT RESEARCH
·
2026
·
IF 1.8
Qiao-Long Hao,Han-Mei Bai,Lei Miao,Shuang Yao,Chuan-Rui Zhang
The first phytochemical investigation of Tarlmounia elliptica (the sole species of its genus) led to the isolation of eight compounds from its aerial parts, including a previously undescribed seco-norlignan, tarlelliptin A (1), and a rare ring B aromatic 1(10→6)abeo-ergostane-type steroid, tarlelliptin B (2). Their structures were fully characterised by comprehensive spectroscopic analysis (HRESIMS, 1D/2D NMR) and corroborated by quantum chemical calculations (including ECD and NMR computations). Biological evaluation for cytotoxic, anti-inflammatory, and antibacterial activities indicated that only the known compound 7-oxo-β-sitosterol (8) exhibited weak antibacterial activity (MIC = 64.0 μg/mL) against methicillin-resistant Staphylococcus aureus (MRSA) strain 252.
✓ 已验证
DOI
PubMed
高影响因子
Journal of Energy Storage
·
2026
·
IF 10.7
Kaixin Xie,Xiaobo Chen
In the pursuit of advanced energy storage solutions, materials featuring high porosity, robust redox activity, and abundant electrochemically accessible sites are highly attractive candidates for next-generation supercapacitor electrodes. In this study, a binder-free positive electrode material consisting of porous MnCo 2 O 4 particles directly coated onto carbon cloth (MnCo 2 O 4 @CC) was synthesized via a straightforward hydrothermal process followed by thermal treatment. Additionally, the incorporation of redox additives into electrolytes has been identified as a promising approach to elevate electrochemical supercapacitor performance. To this end, we designed an aqueous asymmetric supercapacitor (ASC) system utilizing MnCo 2 O 4 @CC and activated carbon (AC) electrodes, where potassium ferricyanide (K 3 [Fe(CN) 6 ], denoted as KFCN) was introduced as an electrolyte additive to augment pseudocapacitive behavior. The electron migration mechanisms and charge storage dynamics of MnCo 2 O 4 @CC in a KOH + KFCN electrolyte were systematically investigated. The MnCo 2 O 4 @CC electrode immersed in a 3 M KOH + 0.05 M KFCN solution exhibited a high specific capacitance of 2206.7 F g −1 at 1 A g −1 , along with low resistance characteristics and exceptional cycling durability. A complete aqueous MnCo 2 O 4 @CC//KOH + KFCN//AC ASC was assembled, demonstrating enhanced specific capacitance, extended cycle life (94.5% capacitance retention after 10,000 cycles), and an ultrahigh energy density of 79.9 Wh kg −1 at 799.6 W kg −1 . These findings provide significant electrochemical insights and demonstrate the great potential of the MnCo 2 O 4 @CC-KOH + KFCN system for next-generation supercapacitor applications.
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2026
·
IF 10.4
Yuzhuo Wei,WenJing Guo,Shiqi Hao,Xueying Sun,Lina Sun,Erke Sun,Xiaotong Li,Ye Jin,Lihua Zhao
2-Amino-1-methyl-6-phenylimidazo [4,5-b] pyridine (PhIP) is a highly polar heterocyclic amine with elevated levels in high-temperature meat products and strong carcinogenicity. This study screened Pediococcus acidilactici X3-1B, achieving a PhIP adsorption rate of 74.30%. The stability of the complex formed between the strain and PhIP was highest at 0 h, 4 °C, and pH 7. Scanning electron microscopy (SEM) revealed significant morphological changes in the strain after PhIP adsorption. Fourier transform infrared spectroscopy (FTIR) results indicated that N H and C O groups on the bacterial surface played a primary role in adsorption. Zeta potential measurements revealed an increase in net negative charge on the bacterial surface following PhIP adsorption. Functional group chemical masking indicated that amino groups are one of the core functional groups influencing X3-1B's adsorption of PhIP. This study provides theoretical foundations and practical references for the application of lactic acid bacteria in food safety.
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2025
·Vol. 26
·期 5
·2027
·
IF 4.9
Shuangliu Chen,Jiřimutu Xiao,Shijie Zhou,Taxi Wumiti,Zitong Zhao,Ruihua Zhao,Yalan Pan,Qing Wang,Yong Ma,Lan Wu,Yang Guo
Dysregulated iron metabolism-induced ferroptosis is considered a key pathological mechanism in the development of osteoporosis (OP). G protein-coupled receptor 30 (GPR30, also known as Gper1) is an estrogen-binding receptor that has shown therapeutic benefits in patients with certain degenerative diseases. Moreover, several studies have demonstrated the anti-ferroptotic effects of estrogen receptor activation. However, its role in the prevention and treatment of OP remains unclear, and there are currently no reports on the anti-ferroptotic function of GPR30 in OP. Therefore, this study aimed to investigate the ferroptosis-related effects and mechanisms of GPR30 in the context of OP. In vivo and in vitro experiments were conducted using wild-type (WT)C57BL/6female mice and GPR30-knockout (GPR30-KO)C57BL/6Jfemale mice. The microarchitecture of the distal femur was assessed using micro-computed tomography (micro-CT), and histomorphological changes were analyzed via hematoxylin and eosin (H&E) staining. Bone marrow mesenchymal stem cells (BMSCs) were isolated and cultured to establish an iron overload model using ferric ammonium citrate (FAC). Interventions included GPR30 overexpression via transfection and BMP-6 inhibition using LDN-214117. Cell viability was evaluated with the CCK-8 assay, while osteogenic differentiation and mineralization levels were assessed using ALP and Alizarin Red S (ARS) staining. Iron accumulation was detected via Prussian blue staining, oxidative stress levels were evaluated using ROS staining, and mitochondrial membrane potential changes were analyzed using JC-1 staining. Transmission electron microscopy (TEM) was employed to observe mitochondrial ultrastructural changes. Additionally, key gene and protein expression levels were measured using immunofluorescence and Western blotting. The micro-CT analysis revealed significant bone microarchitecture deterioration and bone loss in the GPR30-KO mouse model. At the cellular level, GPR30 overexpression markedly reduced iron accumulation and oxidative stress in BMSCs, restored the mitochondrial membrane potential, and improved the mitochondrial ultrastructure. Furthermore, GPR30 enhanced osteogenic differentiation in BMSCs by promoting the activation of the BMP-6/HEP/FPN signaling pathway, leading to increased expression of osteogenic markers. The protective effects of GPR30 were reversed by the BMP-6 inhibitor LDN-214117, indicating that BMP-6 is a critical mediator in GPR30-regulated iron metabolism and ferroptosis inhibition. GPR30 inhibits ferroptosis in BMSCs and enhances osteogenic differentiation by activating the BMP-6/HEP/FPN signaling pathway. This provides new insights and potential therapeutic targets for the treatment of osteoporosis OP.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2025
·
IF 2.5
Qian Liu,Yawen Luo,Xinyi Tao,Qunlin Zhang,Haifeng Lu
As a membrane-bound enzyme, α-Glucosidase (α-Glu) was closely associated with the pathogenesis of type 2 diabetes mellitus, making accurate monitoring of its enzymatic activity and screening of potential inhibitory compounds crucial for both clinical diagnostics and therapeutic development. Herein, we developed a colorimetric platform that synergistically integrates enzymatic catalysis with the redox-responsive optical characteristics of 2,6-dichlorophenolindophenol (DCIP). In this strategy, α-Glu enabled the removal of glucose from 2-O-α-D-glucopyranosyl-L-ascorbic acid to yield ascorbic acid, followed by in situ reduction of DCIP to reduced DCIP (redDCIP). As a result, a dramatic color fading from blue to colorless and a substantial decrease of absorbance at 612 nm were both observed, which was used to both qualitative visual assessment and quantitative spectrophotometric analysis for α-Glu activity. The proposed mechanism was systematically validated through UV-vis spectroscopic characterization and electrospray ionization mass spectrometry analysis. Under optimized experimental conditions, the system demonstrated excellent analytical performance with a good linear response between the absorbance and α-Glu activity over a dynamic range of 2-70 U L⁻¹. The platform achieved a low detection limit of 0.68 U L⁻¹, fulfilling the sensitive monitoring of α-Glu activity. Notably, the proposed approach exhibited several advantages over conventional methods, including user-friendly operation, cost-effective instrumentation and rapid assay without the complex synthesis and modification of nanomaterials, and has been applied to the screening of α-Glu inhibitor obtained from natural plant extracts. Overall, the established sensing platform not only enables sensitive detection of α-Glu activity but also be feasible for its inhibitors screening.
✓ 已验证
产品关联
DOI
高影响因子
Cell Death Discovery
·
2022
·Vol. 8
·期 1
·1-10
·
IF 7.109
Wang Jingyu,Wang Yaqing,Liu Yi,Cai Xintian,Huang Xin,Fu Wenjing,Wang Lei,Qiu Lihua,Li Junying,Sun Li
Ferroptosis is a non-traditional form of regulated cell death, characterized by iron overload and lipid peroxidation. Exploration of ferroptosis in chronic kidney disease (CKD) has been extremely limited to date. In this study, we established a rat model of CKD by 5/6 nephrectomy, treated CKD rats with the ferroptosis inducer, cisplatin (CDDP), and the ferroptosis inhibitor, deferoxamine mesylate (DFO), and observed the resulting pathologic changes (injury markers and fibrosis) and ferroptotic biochemical indices. Kidney iron deposition, lipid peroxidation, mitochondrial defects, ferroptosis marker induction, and TUNEL staining positivity were detected in CKD group rats. Further, treatment with CDDP or DFO influenced renal injury and fibrosis by affecting ferroptosis, rather than apoptosis, and ferroptosis occurs in the remnant kidney due to disordered iron metabolism. In conclusion, our study shows for the first time that 5/6 nephrectomy induces ferroptosis in the remnant kidney and clarifies the underlying pathogenesis. Moreover, we demonstrate that ferroptosis is involved in CKD progression and represents a therapeutic target in chronic kidney injury and renal fibrosis.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）