INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
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2024
·
IF 8.1
Manigandan Ramadoss,Hesheng Yu,Xinsheng Li,Yuanfu Chen
The sluggish electro-kinetics of electron-driven water oxidation restricts the electrocatalyst-assisted electrical to life-valued product conversion applications. Herein, for the first time, self-assembled nanochain network, iron-doped cobalt oxide (Fe–CoO) embedded boron-doped carbon nanotubes (B-CNT), is synthesized via a facile self-assembly-hydrolysis strategy followed by Ostwald-ripening assisted crystal-growth approach. Due to its unique features, such as porous nanochain, low dimensional network, heteroatom distribution, and electronic modulated-rich active sites, it can synergistically accelerate the complicated multistep 4e − electron transfer kinetics, which guarantees Fe–CoO@B-CNT delivering extraordinary OER activity. Herein, a very low Tafel (54.6 mV/dev) and the overpotential of 260 mV to achieve a partial current density of 10 mAcm −2 in alkaline medium. Thus outperforms noble-metal and most cobalt/carbon-based electrocatalysts; furthermore, the hybrid delivers outstanding physicochemical stability over 48 h deprived of degradation. This work gives new insights into the rational designing and self-assembling fabrication of a hetero-atom doped hierarchical nanochain network with high conductivity and abundant active sites to significantly boost water oxidation activity.
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2025
·
IF 4.1
Yan Ma,Peng Zeng,Yan Ma,Xiaomei Yang
Supercapacitors have been extensively studied for their excellent power density and cycle life, but they are limited by low energy density. This study successfully prepared nitrogen/oxygen/boron (N/O/B) co-doped phenolic resin-based porous carbon materials (NBC) through a sol-gel method combined with high-temperature carbonization and activation processes, and systematically investigated their electrochemical performance in supercapacitors. By adjusting the amount of boric acid and the carbonization temperature, the pore structure and heteroatom doping levels of the materials were optimized. Experimental results demonstrate that when the carbonization temperature is 700 °C and the mass ratio of L-tyrosine to boric acid is 1:1, the obtained sample NBC-700-1 exhibits a high specific surface area (1721 m 2 g −1 ), an abundant micro/mesoporous hierarchical structure, and uniform N/O/B doping (N content: 8.31 at.%, O content: 14.11 at.%, B content: 0.55 at.%). In a three-electrode system, NBC-700-1 (377 F g −1 ) exhibits superior specific capacitance, high rate performance (73% capacitance retention at 20 A g −1 ), and long cycle stability at 0.5 A g −1 in 6 M KOH electrolyte compared to NBC-600-1 (300 F g −1 ), NBC-700-0.5 (290 F g −1 ), NBC-700-2 (268 F g −1 ), and NBC-800-1 (351 F g −1 ). Furthermore, a symmetric supercapacitor assembled with Na 2 SO 4 gel electrolyte achieves an energy density of 29.9 Wh kg −1 at a power density of 550 W kg −1 , along with broad temperature adaptability (−25 to 75 °C) and remarkable flexibility (no significant performance degradation after 180° bending). Additionally, the design of a PVA-based gel electrolyte with wide pH applicability provides new insights for the application of such devices in complex environments.
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FEBS Journal
·
2024
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IF 5.5
Yaqing Li,Xiyang Liu,Yulai Li,Jieting Wang,Mengqian Zhang,Weili Xue,Mingzhi Zhang
Diffuse large B-cell lymphoma (DLBCL) is the most common subtype of non-Hodgkin lymphoma and is associated with a poor prognosis. Data from the Gene Expression Profiling Interactive Analysis (GEPIA) database revealed dysregulated expression of several ubiquitin-specific proteases (USPs) in DLBCL tissues (DLBCL vs. non-DLBCL = 47 vs. 337), including USP19 (log 2 fold change = 1.17, P < 0.05). USP19 is closely linked to tumorigenesis, but its role in DLBCL progression remains largely unknown. Here, we investigated the role of USP19 in DLBCL development. Genetic manipulation of USP19 using adenovirus-based vectors was performed in two DLBCL cell lines, SUDHL4 and DB cells. The results showed that USP19 knockdown suppressed the proliferation, anchorage-independent growth and xenograft tumor formation of DLBCL cells and arrested the cell cycle at the G1 stage. In parallel, DLBCL cells overexpressing USP19 acquired a more malignant phenotype. Next, to explore USP19 interactors, we performed co-immunoprecipitation/liquid chromatography–mass spectrometry and identified potential interacting proteins. Among them, Parkinson disease protein 7 (PARK7), a member of the peptidase C56 family known to be involved in carcinogenesis, was further validated to bind with and be stabilized by USP19. Additionally, we found that USP19 induced PARK7 deubiquitylation in both DLBCL cell lines, and PARK7 acted as a downstream effector of USP19 in regulating the growth of DLBCL cells. Collectively, USP19 exerts a tumor-promoting role in DLBCL through interacting with and stabilizing PARK7.
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Foods
·
2024
·Vol. 13
·期 15
·2385
·
IF 4.7
Mian Wang,Yiting Wang,Muhammad Bilal,Chong Xie,Pei Wang,Xin Rui,Runqiang Yang
Isoflavones, a class of substances with high biological activity, are abundant in soybeans. This study investigated isoflavone biosynthesis in soybean cell suspension cultures under UV-B radiation. UV-B radiation enhanced the transcription level and activity of key enzymes involved in isoflavone synthesis in cell suspension cultures. As a result, the isoflavone contents significantly increased by 19.80% and 91.21% in hypocotyl and cotyledon suspension cultures compared with the control, respectively. Meanwhile, a significant difference was observed in the composition of isoflavones between soybean hypocotyl and cotyledon suspension cultures. Genistin was only detected in hypocotyl suspension cultures, whereas glycitin, daidzein, and genistein accumulated in cotyledon suspension cultures. Therefore, UV-B radiation exhibited tissue-specific regulation of isoflavone biosynthesis in soybean cell suspension cultures. The combination of suspension cultures and abiotic stress provides a novel technological approach to isoflavone accumulation.
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JOURNAL OF MEDICAL VIROLOGY
·
2025
·Vol. 97
·期 5
·e70395
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IF 4.6
Qiuhong You,Kaifeng Wang,Zhou Zhao,Heqi Zhou,Zhixian Lan,Hongyan Liang,Rui Deng,Wanying Li,Sheng Shen,Routing Wang,Kaikai Zhang,Dekai Zheng,Jian Sun
In chronic hepatitis B (CHB) patients under antiviral treatment, liver injury, as evidenced by elevated alanine transaminase (ALT), is associated with unfavorable outcomes and needs effective treatment. The interaction between gut microbiota and liver injury in CHB patients remains unclear. Using a case-control design, 28 cases with elevated ALT and 28 matched controls with normal ALT were randomly selected from CHB patients with viral control. Clinical characteristics were comparable between groups. Metagenomic sequencing revealed that Bacteroides fragilis was decreased in cases and exhibited the greatest disparity between cases and controls. Mice colonized by gut microbiota from cases exhibited more severe liver damage in both LPS-induced and MCD diet-induced liver injury models, and had a lower abundance of B. fragilis compared to mice colonized by gut microbiota from controls. Oral gavage of B. fragilis improved both LPS-induced and MCD diet-induced liver injury. Metabolomics analysis revealed that the levels of 7-Ketolithocholic acid (7-Keto-LCA) were positively correlated with B. fragilis and significantly increased in the cultural supernatant of B. fragilis . Consistently, 7-Keto-LCA exerted protective effects against both LPS-induced and MCD diet-induced liver damage. Targeting gut microbiota might be a promising therapeutic treatment for alleviation residual liver inflammation in CHB patients with viral control.
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TISSUE & CELL
·
2025
·
IF 2.5
Minhui Wang,Daojun Chen,Daofa Zhang,Hui Han,Maowei Xie,Ying Zhang,Yun Cao,Jiali Wei
Background Renal fibrosis (RF) is a common histopathological feature of chronic kidney disease (CKD) and is closely associated with persistent inflammation and regulated cell death. However, the underlying molecular mechanisms of pyroptosis in RF are unclear. Methods Pyroptosis-related hub genes in RF were identified through bioinformatics analysis. Granzyme B ( GZMB ) was knocked down in transforming growth factor (TGF-β)-stimulated HK-2 cells and in a folic acid (FA)-induced nephropathy mouse model. Western blot and enzyme-linked immunosorbent assays were performed to detect the expression of fibrosis and pyroptosis-related markers. Cell viability was assessed by the Cell Counting Kit-8 assay. Hematoxylin and eosin (HE) and Masson's staining were used to evaluate tubular injury and collagen deposition in renal tissues. Results GZMB was identified as a pyroptosis-associated hub gene. It was significantly upregulated in RF and exhibited high diagnostic performance. In TGF-β-stimulated HK-2 cells, GZMB knockdown enhanced cell viability and reduced lactate dehydrogenase release. In vivo , GZMB silencing ameliorated tubular injury, reduced collagen deposition, and improved renal function. The expression levels of pyroptosis-related proteins, including the N-terminal fragment of gasdermin D, cleaved caspase-1, and NOD-like receptor family pyrin domain containing 3 (NLRP3), as well as fibrotic markers alpha-smooth muscle actin and collagen type I, were downregulated after GZMB knockdown. Mechanically, GZMB knockdown inhibited NLRP3 inflammasome activation and suppressed the Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) pathway. Conclusion GZMB deficiency inhibits pyroptosis and dampens the JAK2/STAT3 pathway, representing a potential approach for RF treatment.
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ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Hanyue Xue,Min Yu,Kunyi He,Yingnan Liu,Yuanyuan Cao,Yuhang Shui,Jing Li,Muhammad Farooq,Li Wang
Herein, a novel bimodal ratiometric probe for sensitive and selective detection of biothiols (including glutathione (GSH), cysteine (Cys) and homocysteine (Hcys)) was constructed, which was based on the redox reaction between manganese dioxide nanoflakes (MnO 2 NFs) and rhodamine (RhB) and biothiols. When MnO 2 NFs was added into RhB solution, RhB was oxidized to a series of derivatives, accompanying with the colorimetric color changing from purple to light pink and fluorescence changing from red to green. In the presence of GSH, Cys or Hcys, they could reduce MnO 2 NFs to Mn 2+ , thereby preventing the following oxidization of RhB and the corresponding color and fluorescence changes. The absorption intensity ratio and fluorescence intensity ratio showed good linear relationships with the concentrations of biothiols. The colorimetric detection limits for GSH, Cys and Hcys were 0.057 μM, 0.140 μM and 0.087 μM, respectively. And the fluorescence detection limits were 0.177 μM, 0.282 μM and 0.161 μM. More importantly, this probe was successfully applied to monitor the concentration of GSH/Cys/Hcys in human serum samples, with satisfactory recovery. Thus, this MnO 2 NFs-RhB platform can potentially be a candidate for the detection of biothiols.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2019
·
IF 2.931
Lantian Su,Song Wang,Lumei Wang,Zhiyu Yan,Haoyang Yi,Dongwei Zhang,Guoqing Shen,Yun Ma
This paper proposes a fluorescent aptasensor for the detection of carbendazim (CBZ) in aqueous solution using CBZ-specific aptamer as sensing probe, gold nanoparticles (AuNPs) and Rhodamine B (RhoB) as indicator, respectively. In the absence of CBZ, CBZ aptamer could wrap AuNPs and maintained it dispersed in NaCl solution basically. Contrarily, the aptamer could specifically combine with CBZ and form a stable aptamer-CBZ complex, leaving AuNPs exposed to be aggregated by NaCl solution. The dispersed AuNPs could efficiently quench the fluorescence of RhoB, but those aggregated AuNPs have poor capability to impair the fluorescent indicator . Thus, the concentration of CBZ could be detected quantitatively through the distinction of the fluorescence intensity . This convenient fluorescent assay for CBZ had a wide linear range from 2.33 to 800 nM and a 2.33 nM limit of detection (LOD). Furthermore, it had high selectivity over pesticides, antibiotics, metal ions and other disrupting chemicals. As for application, the method could determine CBZ in water samples with recoveries in the range of 96.3–111.2%. This fluorescent aptasensor possessed great potential application for CBZ detection in actual aquatic environment.
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Molecular Therapy-Nucleic Acids
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2021
·
IF 8.886
Jing Miao,Xiqin Yang,Xuwei Shang,Zhe Gao,Qian Li,Yun Hong,Jiaying Wu,Tingting Meng,Hong Yuan,Fuqiang Hu
Hepatitis B (HB) is a viral infectious disease that seriously endangers human health, and since there are no radical drugs to counter this, effective and safe therapies urgently need to be developed. HB virus (HBV) mainly infects hepatocytes (HCs), while the drugs are easily phagocytosed by Kupffer cells (KCs). In this study, the glutathione concentration difference between HCs and KCs was examined and utilized in an ideal drug-release strategy. Here, galactosylated chitosan-oligosaccharide-SS-octadecylamine (Gal-CSSO) was prepared to accurately deliver 10-23 DNAzyme DrzBC (blocking HBeAg expression) or DrzBS (blocking HBsAg expression) in targeted HB therapy. In vitro Gal-CSSO systems exhibited low cytotoxicity, endosomal escape, and glutathione responsiveness. The HBeAg and HBsAg secretion of HepG2.2.15 was significantly decreased by Gal-CSSO systems, and the maximum inhibition rates were 1.82-fold and 2.38-fold greater than those of commercial Lipofectamine 2000 (Lipo2000) systems. In vivo Gal-CSSO systems exhibited HC targeting and HC microenvironmental responsiveness without noticeable hepatotoxicity or systemic toxicity. The HBeAg and HBsAg titers of the HBV-infected mice were evidently decreased by Gal-CSSO systems, and the inhibition rates were 1.52-fold and 1.22-fold greater than those of Lipo2000 systems. This study presents a kind of glycolipid-like polymer micelles that promise efficient and safe gene therapy of HB.
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CHEMISTRY & BIODIVERSITY
·
2025
·
IF 2.5
Qian Luo,Chen Sun,Xue Zhao,Er-Wei Li,Yu-Qing Yi,Yang Zhang,Shuai-Ming Zhu,Yu Yang,Rui Xu,Li-Xia Chen,You-Zhi Zhang,Chang-Wei Li
Two new cytochalasins ( 1 and 2 ) were isolated from the fermentation products of the fungus Xylaria necrophora SZSS1-2-2, along with nine known compounds: compound 3 , arbuschalasin B ( 4 ), cytochalasin C ( 5 ), cytochalasin D ( 6 ), 19,20-epoxycytochalasin C ( 7 ), 19,20-epoxycytochalasin D ( 8 ), cytochalasin R ( 9 ), cytochalasin D 13,14-epoxide ( 10 ), and arbuschalasin A ( 11 ). The structures and absolute configurations of these compounds were elucidated by spectroscopic analyses, electronic circular dichroism, and GIAO 13 C NMR computational methods. All isolated compounds were evaluated for their biological activities, and the results indicated that compounds 5 – 10 exhibited notable cytotoxic effects against the tested cell lines. However, none of the compounds showed antibacterial activity. Graphical
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WATER SCIENCE AND TECHNOLOGY
·
2021
·Vol. 85
·期 1
·354-366
·
IF 1.915
Zhang Kaili,Yang Yanli,Xu Miao,Cheng Tiexin,Zhou Guangdong
In this work, Mackinawite (FeS m ) was synthesized by homogeneous precipitation method, and flower-like nanoparticles formed by the aggregation of nanosheets with a preferred orientation along the (001) plane. The heterogeneous Fenton-like degradation performance of FeS m on rhodamine B (RhB) was investigated, results illustrated that RhB degradation was the synergistic effect of adsorption, Fenton, and a heterogeneous Fenton-like reaction. In repeated experiments, the reduction of reactivity was attributed to the oxidation of FeS m into lepidocrocite, whereas lepidocrocite has relatively low hydroxyl radicals (•OH) production reactivity. Thus, it showed excellent degradation effects in long-time degradation of RhB. Photoluminescence technology and free radical capture experiments demonstrated that •OH produced on the surface of catalyst was the main active species to remove RhB. In addition, the Fe species on the surface of FeS m was the main active center for surface-mediated reactions. A total organic carbon test revealed that the degradation was not complete and degradation intermediates were formed. Liquid chromatography-mass spectrometry technology was used to identify the degradation intermediates. On this basis, possible degradation pathways were proposed. Flower-like Mackinawite (FeS m ) nanoparticles were synthesized using an homogeneous precipitation method. FeS m was used as an heterogeneous Fenton-like catalyst to degrade RhB. The reduction of FeS m reuse activity was mainly attributed to the formation of lepidocrocite with lower activity. •OH surface-bounded of FeS m was the main active species for RhB degradation. The possible degradation pathway of RhB was speculated.
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APPLIED SURFACE SCIENCE
·
2024
·
IF 6.3
Guohua Zhu,Xiaoyan Zhang,Yongguang Liu,Fangbo Ma,Jing Suo,Ling Wang,Shan Liu
Layered oxide cathodes have attracted widespread attention due to their high-voltage and high discharge capacity. Nevertheless, the irreversible redox reactions of layered oxide cathode and decomposition of electrolyte at high-voltage greatly reduce the reversibility of the batteries. Herein, we employ a double-doping (Li&B) strategy to enhance the electrochemistry performance of the layered NaNi 0.3 Fe 0.3 Mn 0.4 O 2 (NNFM) cathode at high voltage. It proved that the dissolution of the transition metal can be restricted by double doping, and the irreversible phase transition is mitigated even at high voltage. As a result, battery with the double-doping cathode exhibits excellent electrochemistry performance, which achieves a capacity of 175 mAh g −1 at 0.2C, maintaining a capacity retention of approximately 87.3% over 100 cycles at 1C, and demonstrates exceptional rate performance. This work presents a novel insight into the doping technique for the high-performance cathode materials and offers a general method for the high energy Na-ion batteries.
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