JOURNAL OF HAZARDOUS MATERIALS
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2026
·
IF 10.6
Yuan Wang,Lingxiu Liu,Fan Chen,Shaoyu Fang,Xiaohai Yan,Wushuang Bai,Qinglin Sheng,Yahong Yuan,Kewei Feng,Tianli Yue
This study developed two novel magnetic nanocomposites with immobilized porcine pancreatic lipase (PPL) for simultaneously removing patulin (PAT) and ochratoxin A (OTA)—two hazardous environmental contaminants—from fruit juices. All detoxification experiments were conducted in real fruit juice matrices (apple juice for PAT, grape juice for OTA) under ambient temperature (25 °C) with realistic contamination ranges. The results demonstrated MNP@UiO-66-(SH)₂@PPL achieved removal rates of 77.03% for PAT in apple juice and 45.03% for OTA in grape juice, while MNP@UiO-66-NH₂@PPL showed removal rates of 85.74% for OTA and 48.44% for PAT. LC-MS analysis indicated that PAT undergoes ring-opening to ascladiol, while OTA is hydrolyzed to phenylalanine and OTα. Molecular docking revealed that PPL spontaneously binds to both toxins via hydrogen bonding and hydrophobic interactions. CCK-8 assays confirmed low cytotoxicity of the materials and their degradation products, with no adverse impact on juice quality. The composites also exhibited magnetic recovery rates > 93%, enabling facile separation and recyclability. This work provides a safe, efficient, and reusable strategy for multi-mycotoxin decontamination with demonstrated environmental relevance.
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POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2025
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IF 6.8
Siyu Chen,Yiwen Zhang,Shuang Liu,Juan Ma,Huiling Wang,Wei Chang,Hengfeng Li,Xiaochun Ding,Xuejun Pan,Xuewu Duan
DNA methylation is a key epigenetic regulator of gene expression, yet its role in pigment and ascorbic acid (AsA) metabolism in Rosa roxburghii remains unclear. This study examined the effects of 5-azacytidine (5-azaC), a DNA methyltransferase inhibitor, on postharvest fruit quality after cold storage. 5-azaC treatment reduced decay, delayed the onset of color change, preserved overall fruit brightness, maintained firmness, and suppressed respiration during cold storage. It inhibited chlorophyll a degradation and enhanced zeaxanthin, lutein, α-carotene, phytoene, and violaxanthin levels. 5-azaC enhanced AsA content, accompanied by increased activities of monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), glutathione reductase (GR), ascorbate oxidase (AO), and ascorbate peroxidase (APX). Integrated RNA-seq and WGBS analysis showed that 5-azaC induced carotenoid biosynthesis genes ( RrBCH, RrVDE, RrNSY, RrDXS and RrZE P), with RrVDE hypomethylated. RrMDHAR, RrDHAR, RrAO1, RrGR, RrGALUR, RrGalDH and RrIMPL2 were upregulated by 5-azaC, with reduced DNA methylation levels observed in RrAO1, RrGalDH and RrIMPL2 . Transcription factors RrAP2, RrMYB21, RrMYB44 and RrPosF21-like were induced by 5-azaC, with RrMYB21 hypomethylated. DNA demethylases ( RrROS1 and RrROS1-like ) were upregulated, while methyltransferases ( RrCMT-like, RrMET, RrMDR2 and RrDMR2-like ) were downregulated. Silencing RrAP2, RrMYB21, RrMYB44, RrPosF21-like and RrROS1-like reduced AsA content and downregulated AsA metabolism-related genes in R. roxburghii fruit . These findings provide preliminary evidence that 5-azaC may regulate pigment and AsA metabolism via epigenetic mechanisms during cold storage.
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NEW JOURNAL OF CHEMISTRY
·
2022
·Vol. 47
·期 2
·828-837
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IF 3.925
Tiantian Wan,Zulei Zhang,Hailong Wang,Yiwen Yang,Hanxing Yang,Jian Zhang,Yanbo Zeng,Lei Li,Jinchun Li
Chlorogenic acid (CGA), a cis-diol-containing compound, exhibits anti-inflammatory, antiviral, antimicrobial and anti-oxidation properties. Thus, selective determination of CGA in food products is extremely necessary. In this study, a boron affinity magnetic polymer named Fe3O4@SiO2@PIL was synthesized to detect the concentration of CGA in actual fruit samples. The Fe3O4@SiO2@PIL was characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared (FT-IR) spectroscopy, X-ray photoelectron spectroscopy (XPS) and thermogravimetric (TG) analysis. The results showed that the Fe3O4@SiO2@PIL has uniform morphology, good magnetic properties, suitable structure and high stability. The experimental assays showed that the Fe3O4@SiO2@PIL can detect the concentration of CGA within 7 min, and has a good linear relationship with CGA in the concentration range of 0.025–2 μM (R2 = 0.9989) with a detection limit of 10 nM (S/N = 3). Moreover, the Fe3O4@SiO2@PIL exhibited excellent selectivity and can be reused for at least 5 runs, indicating its good stability and reusability. Finally, the Fe3O4@SiO2@PIL was used to selectively separate CGA in real fruit samples with the recovery ranging from 93.4% to 103.0% and the relative standard deviation (RSD) between 0.7% and 5.1%. This study gives a new insight into the utilization of magnetic materials by grafting boric acid functionalized ionic liquids for specific and rapid detection of natural cis-diol-containing compounds.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2023
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IF 6
Chao Zhang,Xiaoai Chen,Xiaoxue Guo,Ruijia Guo,Lin Zhu,Xinrong Qiu,Xiaohan Yu,Jun Chai,Chunhe Gu,Zhen Feng
Noni ( Morinda citrifolia L.) fruit juice (NFJ) has an unpleasant flavor but is highly bioactive, so it is necessary to improve the quality of NFJ by exploring a novel strategy. The effects of eight lactic acid bacteria (LAB) on the antioxidant, phenolic, iridoid, and flavor properties of NFJ were investigated in the present study. The results showed that antioxidant activity was improved after NFJ fermentation by LAB and that L. rhamnosus had the highest free radical scavenging activity. The highest total phenolic content and total flavonoid content were in L. brevis- fermented NFJ (78.85 mg gallic acid equivalent/100 mL and 37.12 mg rutin equivalent/100 mL, respectively). Bioconversion of iridoid revealed that deacetylasperulosidic acid can be deacetylated from asperulosidic acid. Furthermore, LAB fermentation presented a significant decrease in unpleasant butanoic acid, with the lowest content found in L. fermentum- fermented NFJ. This research provides progressive information on the application of LAB culture in NFJ fermentation.
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FOOD CHEMISTRY
·
2022
·
IF 9.231
Guo-Dong Zhuang,Wen-Ting Gu,Shu-Hong Xu,Dong-Min Cao,Si-Min Deng,Yong-Sheng Chen,Shu-Mei Wang,Dan Tang
A new AAPH-Incubating HPLC-DAD-HR MS/MS method was developed for the rapid and high-throughput screening of antioxidants directly in natural products and applied to Gardenia jasminoides fruit. This method was assumed that the peak areas of compounds with potential antioxidant activity in HPLC chromatograms would be significantly reduced or disappeared after incubating with the AAPH which can release ROO at physiological conditions (37 °C, pH 7.4). Additionally, the activity of antioxidants can be evaluated by comparing the peak reduction rates and the screened components can be further identified by HRMS/MS. Then, 17 potential natural antioxidants from the crude extract of GJF was screened. Among them, three major components including crocin I, crocin II and crocetin showed excellent ROO scavenging activity, which were further validated by the ORAC assay. In conclusion, our study provided a simple and effective strategy to rapidly screen antioxidants in natural products.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2022
·
IF 9.136
Shengrui Xu,Hailin Liu,Anying Long,Suling Feng,Chang-Po Chen
An in-situ synthesis strategy was proposed for fabrication of carbon dots embedded wrinkled mesoporous silica (CDs/WMS) microspheres. Characterizations results demonstrated that the doping of CDs with a moderate content still allowed the microspheres to maintain wrinkled and highly mesoporous structures. The CDs/WMS composite was subsequently employed as solid phase microextraction (SPME) coating for sensitive determination of ultra-trace organochlorine pesticides (OCPs) from aqueous solutions. High surface area, abundant chemical groups, and plentiful porosity made CDs/WMS microspheres present superior extraction capacities for OCPs compared with commercial SPME coatings and pure WMS. Under the optimum extraction conditions, the proposed analytical method exhibited wide linearity range (0.2–1000 ng/L), low detection of limits (0.0023–0.073 ng/L for water, 0.0062–0.41 ng/L for fruit juice), and satisfactory reproducibility with relative standard deviations of 6.2 %−13.3 % and 7.3 %−13.2 % for water and fruit juice, respectively. Finally, the proposed method was successfully used for determination of OCPs in real water and fruit juice samples. The recoveries varied from 80.1 % to 105.2 % for water and 71 % to 106.8 % for fruit juice. This study developed a novel strategy for synthesis of CDs/WMS microspheres, and proposed a new type of SPME coating, which expanded the potential of CDs in capturing organic pollutants from aqueous solutions.
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FOOD CHEMISTRY
·
2022
·
IF 9.231
Zhimin Zhang,Miao Liang,Binshan Liu,Yajian Wu,Rui Wang,Yuping Liu
To characterize the key odorants in ripe Capparis spinosa L. fruit (RCF) and to investigate changes in the odorants after storage, volatiles were captured by both solid-phase microextraction and solvent extraction combined with solvent-assisted flavor evaporation; a total of 22 aroma-active regions were detected in the isolates by gas chromatography–olfactometry. Of 19 odor-active compounds identified positively, 13 odorants were found in RCF for the first time; their flavor dilution factors ranging from 1 to 512 were measured by an aroma extract dilution assay. Eleven odorants were determined as the key odorants by stable isotope dilution analysis, odor activity value (OAV) calculation, and recombination experiments. To further investigate the changes in odorants after storage, their concentrations and OAVs were determined. The results showed that the number of key odorants did not change before and after storage, but the concentrations of octanoic acid, sotolon, and 3-methylbutanoic acid increased markedly.
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2022
·
IF 4.598
Wei Zhong,Jin Zou,Yanyu Xie,Jing Yang,Mingfang Li,Shuwu Liu,Yansha Gao,Xiaoqiang Wang,Limin Lu
Herein, a highly sensitive and selective electrochemical sensor for the detection of benomyl (BN), a typical benzimidazole fungicide was proposed based on hierarchical nano-Cu x O decorated MWCNTs-COOH/MXene (nano-Cu x O-MWCNTs-COOH /MXene) composite. The ternary heterostructure presents good electrical conductivity, large specific surface area, and high catalytic activity, exhibiting excellent electrocatalytic activity with enhanced electrochemical signals towards the oxidation of BN. Besides, the embedded nano-Cu x O particles possess strong affinity toward benzimidazole molecules, which could promote the preconcentration of BN, further enhancing the sensitivity, as well as ensuring the high selectivity. Benefitting from the synergistic effect of components in the composite, the developed sensor presents a broad linear range from 10.0 nM to 10.0 μM with a low limit of detection of 3.0 nM (S/N = 3). Meanwhile, the nano-Cu x O-MWCNTs-COOH/MXene electrode also displays good long-term stability and repeatability. Furthermore, this method was successfully applied for the detection of BN in apple samples, illustrating its promising practicability.
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Food & Function
·
2022
·Vol. 13
·期 17
·8880-8891
·
IF 6.317
Juan Su,Xiong Fu,Qiang Huang,Guang Liu,Chao Li
The aim of this study was to elucidate the liberation and phytochemical profile of bound polyphenols present in dietary fiber (RPDF) isolated from Rosa roxburghii fruit pomace during in vitro-simulated digestion and colonic fermentation. The variation in the biological activity and prebiotic potential of the released polyphenols from RPDF was investigated. The results showed that the bound polyphenols were released from RPDF at a higher ratio during colonic fermentation than gastrointestinal digestion. The released polyphenols showed antioxidant activity and α-glucosidase inhibitory activity. The in vitro colonic fermentation assay showed that compared with dephenolized RPDF (DP-RPDF), RPDF exhibited a significantly stronger prebiotic effect by lowering the Firmicutes to Bacteroidetes (F/B) ratio at the phylum level, increasing the relative abundances of beneficial bacteria at the genus level, and improving the production of short chain fatty acids (SCFAs). These findings indicate that the bound polyphenols may act as important functional food ingredients that can contribute to the biological properties of RPDF. In addition, this study provides new insights into the high-value utilization of Rosa roxburghii fruit pomace, which will reduce the wastage of resources and greatly contribute to environmental protection.
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FOOD CHEMISTRY
·
2022
·
IF 9.231
Xiaojiao Du,Wenhan Du,Jun Sun,Ding Jiang
Herein, a high-performance self-powered photoelectrochemical (PEC) sensor was fabricated for chlorpyrifos (CPF) assay based on the noble-metal-free AgBr/Ti 3 C 2 Schottky interface in fruit and vegetables. The Schottky barrier could provide an electron-transfer irreversible passage from AgBr to Ti 3 C 2 nanosheets, and thus improving the light absorption and efficiency of charge separation. Such Schottky interface exhibited an ultrasensitive and selective photocurrent response for CPF due to the metal–ligand charge transfer effect. Because Ti 3 C 2 MXene nanosheet had coordinative interaction with CPF and thus suppressed its photocurrent response of the Schottky interface, which was further confirmed by the electrochemical impedance spectra and X-ray photoelectron spectroscopy. The developed self-powered PEC sensor showed wide linear range (1 × 10 −3 ∼ 1 ng L −1 ), low detection limit (0.33 pg L −1 ), limit of quantitation (1 pg L −1 ), good reproducibility and stability. The sensor was applied for the determination of CPF in river water, apple and cucumber samples with good satisfactory results.
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FOOD CHEMISTRY
·
2023
·
IF 8.8
Zhiyang Chen,Yue Sun,Jiyong Shi,Wen Zhang,Xinai Zhang,Xiaowei Hang,Zhihua Li,Xiaobo Zou
The goal of food safety supervision is to directly identify the pesticide residues on the surface of fruits and vegetables. This study proposed to develop a facile, non-destructive, and sensitive method based on surface-enhanced Raman scattering (SERS) to detect non-systemic pesticides on the surface of fruits and vegetables. The composite material was prepared by loading CTAB guided Au@Ag NRs with positive charge onto filter paper which was modified with PDADMAC(+) and PSS(−) using electrostatic adsorption. Au@Ag NRs with bimetallic synergies were effectively adsorbed in the fiber grid to generate 3D SERS hotspots within a few microns of depth. The results showed that the 3D composite flexible substrate had a high SERS activity, great repeatability, and sensitivity when the method was utilized to detect 4-MBA, methyl-parathion, thiram and chlorpyrifos. Three kinds of non-systemic pesticides on the peel could be detected directly and quickly owing to the arbitrary bending of the substrate, demonstrating the efficiency of the SERS “paste-reading” method. The acquired findings demonstrated that PDADMAC/PSS/Au@Ag NRs composite filter paper had the potential to provide rapid feedback for in- situ analysis of pesticide residues on the surface of fruit and vegetable.
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Journal of Cleaner Production
·
2023
·
IF 11.1
Yuwei Hua,Donghui Xu,Zhongxiao Liu,Jie Zhou,Jiatong Han,Zhihao Lin,Dan Xu,Ge Chen,Xiaodong Huang,Jing Chen,Jun Lv,Guangyang Liu
To deal with the environmental pollution caused by dyes and heavy metals , it is vital to design and prepare adsorbents with efficient adsorption and removal capabilities for the regulated removal of contaminants in the environment. In this work, biomimetic apatite hydrogel beads inspired by the structure of petunia pollen were produced in order to efficiently remove lead ions (Pb 2+ ) and malachite green (MG) by adsorption. Utilizing an ionic cross-linking approach, magnetic hydroxyapatite nanoparticles (MHNPs) were encapsulated in sodium alginate gels to make hydrogel beads (MHSB) with the structure of petunia pollen. The MG and Pb 2+ adsorption capacities of MHNPs were 208.06 mg/g and 475.50 mg/g, respectively, demonstrating a satisfactory adsorption ability. In addition, the magnetic hydroxyapatite nanoparticles/hydrogel microspheres demonstrated effective MG and Pb 2+ adsorption and removal in mixed adsorption studies, environmental water samples, and fruit juices, as well as in real application. Due to their sound adsorption capability and stability, magnetic MHNPs and MHSB are regarded as fairly promising composite materials for the adsorption and removal of dyes and heavy metal pollutants.
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