Journal of Materials Chemistry A
·
2014
·Vol. 2
·期 33
·13277-13282
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IF 0
Ru-Jun Chen,Mian Huang,Wen-Ze Huang,Yang Shen,Yuan-Hua Lin,Ce-Wen Nan
Li–La–Zr–O thin films were successfully fabricated using a sol–gel spin coating method, and the effects of altering the annealing temperature and the number of layers of the films on electrolyte conductivity were studied. Using X-ray diffraction, scanning electron microscopy and X-ray photoelectron spectroscopy, the characteristics of these films were investigated as a function of annealing temperature and the numbers of layers of the film. With these methods, an amorphous structure and a smooth surface on the films were revealed. The thin film's ionic conductivity was investigated by impedance analysis. The results show that the ionic conductivity of the films decreases with an increase of the annealing temperature, from 1.67 × 10−6 S cm−1 for 600 °C annealing to 8.53 × 10−7 S cm−1 for 800 °C annealing. The influence of film thickness on conductivity was investigated and the conductivity followed an inverse u-shaped curve with increasing film thickness. We propose that Li–La–Zr–O thin films may be promising solid electrolytes for solid-state lithium-ion batteries.
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Frontiers in Plant Science
·
2016
·
IF 4.495
Xu Huanhuan,Wang Ya,Chen Yana,Zhang Pan,Zhao Yi,Huang Yewei,Wang Xuanjun,Sheng Jun
Galloylated catechins, as the main secondary metabolites in the tea plant, including (-)-epigallocatechin-3-gallate and (-)-epicatechin-3-gallate, comprise approximately three-quarters of all the tea plant catechins and have stronger effects than non-galloylated catechins, both on the product quality in tea processing and the pharmacological efficacy to human beings. The subcellular localization of galloylated catechins has been the primary focus of studies that assess biosynthesis and physiological functions. Classical histochemical localization staining reagents can not specifically detect galloylated catechins; thus, their subcellular localization remains controversial. In the present study, we generated a monoclonal antibody (mAb) against galloylated catechins, which can be used for the subcellular localization of galloylated catechins in the tea plant by immunohistochemistry. Direct ELISA and ForteBio Octet Red 96 System assay indicated the mAb could recognize the galloylated catechins with high specificities and affinities. In addition, tea bud was ascertained as the optimal tissue for freezing microtomic sections for immunohistochemistry. What’s more, the high quality mAbs which exhibited excellent binding capability to galloylated catechins were utilized for the visualization of them via immunohistochemistry. Our findings demonstrated that vacuoles were the primary sites of localization of galloylated catechins at the subcellular level.
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ACS Catalysis
·
2016
·
IF 9.307
Nengchao Luo,Min Wang,Hongji Li,Jian Zhang,Huifang Liu,Feng Wang
One of the challenges of depolymerizing lignin to valuable aromatics lies in the selective cleavage of the abundant C–O bonds of β-O-4 linkages. Herein we report a photocatalytic oxidation–hydrogenolysis tandem method for cleaving C–O bonds of β-O-4 alcohols. The Pd/ZnIn2S4 catalyst is used in the aerobic oxidation of α-C–OH of β-O-4 alcohols to α-C═O with 455 nm light, and then a TiO2–NaOAc system is employed for cleaving C–O bonds neighboring the α-C═O bonds through a hydrogenolysis reaction by switching to 365 nm light. Interestingly, the oxidation–hydrogenolysis tandem reaction can be conducted in one pot to offer ketones and phenols (up to 90% selectivity) via a dual light wavelength switching (DLWS) strategy. EPR and metal loading experiments elucidate that Ti3+ in TiO2 is formed in situ and is responsible for the photocatalytic hydrogenolysis through electron transfer from Ti3+ to the β-O-4 ketones.
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RSC Advances
·
2016
·Vol. 6
·期 92
·89484-89491
·
IF 3.289
Yingshuai Liu,Jin Xie,Zeying Zhang,Zhisong Lu
O-GlcNAcylation is a very important post-translational modification of nucleocytoplasmic proteins. Aberrant O-GlcNacylation has been found in a variety of human diseases including many types of cancers, diabetes, and Alzheimer's disease. However, analysis of protein O-GlcNacylation still remains a great challenge due to its low levels and labile nature. Herein, a highly sensitive and reliable colorimetric method is developed by use of wheat germ agglutinin (WGA) lectin for specific recognition of O-GlcNAc and gold nanoparticle (AuNP)-catalyzed copper deposition for signal amplification. In presence of the target molecule, AuNP is specifically introduced onto the sensing surface and then catalyzes the copper deposition. An Au@Cu core–shell structure is produced from the deposition. The obtained copper shell on AuNP is dissolved by FeCl3 due to the oxidation–reduction reaction between copper and Fe3+, resulting in Cu2+ and Fe2+, which subsequently form a red coordination complex in the presence of bathophenanthrolinedisulfonic acid disodium salt (BPT2−). Thus, the amount of the red complex is proportional to the level of O-GlcNAc. Qualitative O-GlcNAc detection is realized by direct readout with the naked eye, while the quantitative analysis is achieved by measurement of the absorbance using a UV-Vis spectrophotometer. With increasing time, more and more copper is deposited on the surface of AuNP, thus achieving significant signal amplification. Under optimal conditions, an ultralow limit of detection (LOD) down to 0.02 pg mL−1 is achieved with GlcNAc-conjugated BSA as a model analyte. The developed platform was also successfully applied to analyze O-GlcNAcylation levels in prostate cancer cell line PC-3 and normal cell line RWPE-1. The results obtained by the proposed method are in good agreement with the data from protein microarray assays, demonstrating its great potential for sensitive and reliable O-GlcNAcaylation detection in fundamental research and in cancer diagnosis.
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高影响因子
FOOD HYDROCOLLOIDS
·
2017
·
IF 4.747
Ping Shao,Huiling Ma,Jieyu Zhu,Qiang Qiu
The aim of this research was to investigate the effect of ionic strength (Na + 0.1–0.5 mol/L, Ca 2+ 0.01–0.05 mol/L) on the physicochemical stability of o/w emulsions stabilized by Ulva fasciata polysaccharide . Droplet diameter, Polydispersity Index (PDI), zeta-potential value, microscopic morphology and rheological properties were achieved to investigate the possible stability mechanisms. The results showed that different metal ions had significant effects on the stability of o/w emulsions. When compared the effect of Ca 2+ with that of Na + , it was found that Na + concentration did not have a significant effect on emulsions stability. The results indicated that zeta-potential decreased sharply with increasing calcium ionic strength (CaCl 2 :0.01 mol/L - 0.05 mol/L) and the minimum zeta-potential value was −27.5 mV, while zeta-potential changed slightly when NaCl (0.1 mol/L - 0.5 mol/L) were added. As for z-average size and PDI, they changed significantly with higher valence metallic ions. With increasing NaCl and CaCl 2 concentration, the retention rate of R-(+)-Limonene was reduced and emulsions exhibited non-Newtonian behavior. Another interesting result was that the addition of Ca 2+ at low concentration (0.01 mol/L - 0.02 mol/L) managed to improve the stability of emulsions .
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Nanoscale
·
2017
·Vol. 9
·期 44
·17593-17600
·
IF 7.367
Min Wang,Xiangqian Fan,Lingxia Zhang,Jianjun Liu,Beizhou Wang,Ruolin Cheng,Mengli Li,Jianjian Tian,Jianlin Shi
Porous activated carbons (PACs) are promising candidates to capture CO2 through physical adsorption because of their chemical stability, easy-synthesis, cost-effectiveness and good recyclability. However, their low CO2 adsorption capacity, especially low CO2/N2 selectivity, has limited their practical applications. In this work, an optimized PAC with a large specific surface area, a small micropore size, and a large micropore volume has been synthesized by one-step carbonization/activation of casein using K2CO3 as a mild activation agent. It showed a remarkably enhanced CO2 adsorption capacity as high as 5.78 mmol g−1 and an excellent CO2/N2 selectivity of 144 (25 °C, 1 bar). Based on DFT calculations and experimental results, the coexistence of adjacent pyridinic N and –OH/–NH2 species was proposed for the first time to make an important contribution to the ultra-high CO2 adsorption performance, especially CO2/N2 selectivity. This work provides effective guidance to design PAC adsorbents with high CO2 adsorption performance. The content of pyridine N combined with –OH/–NH2 was further elevated by additional nitrogen introduction, resulting in a further enhanced CO2 adsorption capacity up to 5.96 mmol g−1 (25 °C, 1 bar). All these results suggest that, in addition to the well-defined pore structure, pyridinic N with neighboring OH or NH2 species played an important role in enhancing the CO2 adsorption performance of PACs, thus providing effective guidance for the rational design of CO2 adsorbents.
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PubMed
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2017
·Vol. 40
·期 23
·4653-4660
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IF 2.557
Haoran Fan,Jinpeng Wang,Qingran Meng,Yaoqi Tian,Xueming Xu,Zhengyu Jin
Photoirradiation surface molecularly imprinted polymers for the separation of 6- O -α- d -maltosyl-β-cyclodextrin were synthesized using functionalized silica as a matrix, 4-(phenyldiazenyl)phenol as a light-sensitive monomer, and 6- O -α- d -maltosyl-β-cyclodextrin as a template. Fourier transform infrared spectroscopy results indicated that 4-(phenyldiazenyl)phenol was grafted onto the surface of functionalized silica. The obtained imprinted polymers exhibited specific recognition toward 6- O -α- d -maltosyl-β-cyclodextrin. Equilibrium binding experiments showed that the photoirradiation surface molecularly imprinted polymers obtained the maximum adsorption amount of 6- O -α- d -maltosyl-β-cyclodextrin at 20.5 mg/g. In binding kinetic experiments, the adsorption reached saturation within 2 h with binding capacity of 72.8%. The experimental results showed that the adsorption capacity and selectivity of imprinted polymers were effective for the separation of 6- O -α- d -maltosyl-β-cyclodextrin, indicating that imprinted polymers could be used to isolate 6- O -α- d -maltosyl-β-cyclodextrin from a conversion mixture containing β-cyclodextrin and maltose. The results showed that the imprinted polymers prepared by this method were very promising for the selective separation of 6- O -α- d -maltosyl-β-cyclodextrin.
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CHINESE JOURNAL OF CHEMICAL ENGINEERING
·
2017
·
IF 1.174
Jianhua Chen,Xue Sun,Lijing Lin,Xinfei Dong,Yasan He
In the present paper, a metal–organic framework Cr-BDC was prepared and used as adsorbent for adsorption of o -nitrophenol (ONP) and p -nitrophenol (PNP) from aqueous solutions. Cr-BDC was characterized by scanning electron microscopy, transmission electron microscope, X-ray diffraction and BET methods. The results indicate that Cr-BDC gets a very large specific surface area of 4128 m 2 ·g − 1 and pore sizes are concentrated in 1 nm, which is a benefit for using for wastewater treatment . The influences of the adsorption conditions, such as temperature, solution concentration, adsorption time and reusability on adsorption performance were investigated. Cr-BDC exhibited an encouraging uptake capacity of 310.0 mg·g − 1 for ONP, and adsorption capacity of Cr-BDC for ONP is significantly higher than that for PNP under suitable adsorption conditions. The characterizations of adsorption process were examined with the Lagergren pseudo-first-order, the pseudo-second-order kinetic model, and the intra-particular diffusion model. Kinetics experiments indicated that the pseudo-second-order model displayed the best correlation with adsorption kinetics data. Furthermore, our adsorption equilibrium data could be better described by the Freundlich equation. The results indicate that the as-prepared Cr-BDC is promising for use as an effective and economical adsorbent for ONP removal.
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高影响因子
BIOORGANIC CHEMISTRY
·
2024
·
IF 4.5
Fengxin Zheng,Jiale Ke,Shiqin Lin,Wenjie Ye,Zhenkun Wu,Yuexin Xu,Suiqing Mai,Yishuang Chen,Zitao Guo,Huazhong Hu,Shuqin Zhang,Jianxin Pang,Qun Zhang,Zean Zhao
Inhibition of human concentrative nucleoside transporter 2 (CNT2) could suppress increases in serum urate levels derived from dietary purines. However, the structural basis for substrate recognition of CNT2 is still unknown and only a few inhibitors have been reported. In this study, a homology model of CNT2 was constructed and residues T315, E316, N426, N491, E492, F536 and N538 were identified as binding sites for adenosine through site-directed mutagenesis and a 3 H-adenosine uptake assay. To explore potential CNT2 inhibitors, a database containing 4704 saccharides and glycosides was constructed, followed by high-throughput virtual screening. The top 20 compounds with the highest docking scores were then evaluated their in vitro activity. Among them, cyanidin-3-O-galactoside (Cy3Gal) demonstrated the most potent inhibitory activity against CNT2, exhibiting an IC 50 value of 9.40 μM. Docking analysis revealed that residues M314, T315, T347, N422, F536 and S541 contributed to a strong binding affinity with Cy3Gal. In addition, Cy3Gal exhibited minimal inhibitory effects on CNT3 and equilibrative nucleoside transporters (ENTs) in vitro . At doses of 5–20 mg/kg, Cy3Gal effectively reduced serum and urine levels of uric acid and adenosine in vivo . The CCK-8 assay revealed that Cy3Gal displayed minimal cytotoxicity to mTEC and hIEC cells at a concentration of 100 μM. The serum CR and BUN levels indicate that Cy3Gal does not exhibit any apparent renal toxicity compared to lesinurad and allopurinol. HE examination showed no noticeable pathological changes in the kidneys or colons after treatment with Cy3Gal. In summary, Cy3Gal could be a CNT2 inhibitor with favorable drugability for the treatment of hyperuricemia.
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ACS Sustainable Chemistry & Engineering
·
2024
·
IF 7.1
Xinyu Mao,Yu Chen,Shihao Li,Yang Yang,Chen Tao,Jiafu Shi,Zhongyi Jiang
Activation of the N≡N bond is of importance in photocatalytic nitrogen (N2) fixation, which strongly relies on the precise construction of catalytic active sites. Herein, we reported a dual-metal photocatalyst of Mo/UN(Zr) with a high proportion of Mo-coordinated Zr–O clusters (Zr–O–Mo dual-metal active sites) for photocatalytic N2 fixation, which was synthesized though post-modification to the defects of UN(Zr) by Mo. By contrast with the dual-metal photocatalyst of UN(Zr–Mo) synthesized via the co-mixing method, Mo/UN(Zr) possesses a higher proportion of Zr–O–Mo dual-metal active sites that is crucial for activating and polarizing N≡N bond as evidenced by in situ Fourier transform infrared (FTIR) spectroscopy combined with density functional theory (DFT) calculations. The synergistic effect of Zr–O–Mo dual-metal active sites resulted in a lower potential activation energy barrier for N2 activation which promoted the photocatalytic N2 fixation efficiency. The optimum photocatalytic N2 fixation rate of Mo/UN(Zr) was 255.7 μmol g–1 h–1 by adjusting the loading amount of Mo, which was enhanced about 2.5 times and 1.6 times compared with UN(Zr) and UN(Zr–Mo) photocatalyst, respectively. Our study will afford valuable guidance for designing efficient photocatalysts for photocatalytic N2 fixation.
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npj Science of Food
·
2025
·Vol. 9
·期 1
·1-9
·
IF 7.8
Liu Kai,Lian Yinyin,Xie Xingyun,Gao Yuyang,Song Jiajia,Suo Huayi,Zhang Yu
This study investigates the impact of O. oeni on the quality of blueberry wine, employing various analytical methods to determine parameters such as alcohol content, antioxidant capacity, organic acids, amino acids, and volatile components. The results demonstrated that co-inoculation with O. oeni significantly decreased the titratable acidity of blueberry wine. Among the organic acids, the malic acid content of ST and SE groups decreased by (26.24 ± 1.24)% and (35.75 ± 4.02)%, respectively. The main consumed amino acids were aspartate, lysine, and arginine after co-inoculation with O. oeni . Additionally, co-inoculation with O. oeni significantly increased the content of medium-chain fatty acid ethyl esters, especially isoamyl acetate content. The antioxidant capacity of blueberry wine was significantly enhanced by co-inoculation with O. oeni , particularly in terms of measurement of 1,1-diphenyl-2-picryl-hydrazyl (DPPH) clearance. The findings of this study provide a solid basis for improving the quality of blueberry wine and promoting the growth of blueberry wine industry.
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高影响因子
APPLIED SURFACE SCIENCE
·
2025
·
IF 6.9
Fengyi Wang,Yihao Wang,Jinshuo Zou,Gemeng Liang,Kaiqi Hou,Lining Liu,Zehui Lin,Ci Song,Shengyu Liu,Xingyun Li
Crystal structure and surface modulation of Co 3 O 4 catalyst holds great promise for the improvement of catalytic performance for propane oxidation. In this study, Mn doping in Co 3 O 4 was first adapted to introduce crystal defects. And acid etching was then used to orchestrate the surface properties of Mn doped Co 3 O 4 . The combination of both Mn doping and acid etching resulted in a distorted crystal structure, which significantly activates the Co-O bonds, obviously increasing the exposed active oxygen and enhancing the intrinsic oxidation ability. Consequently, as a catalyst for propane oxidation, Mn-Co 3 O 4 -H facilitates the acetate intermediate generation and its decomposition, resulting in an excellent oxidation activity with propane conversion rate 3.8 times higher than that of the pristine Co 3 O 4 catalyst at reaction temperature of 195 °C. Meanwhile, the catalyst demonstrates a stable hydrothermal performance with negligible deactivation during 30 h on-stream test. This study set an important paradigm for the structure and surface manipulation of Co 3 O 4 catalyst for efficient propane oxidation.
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