BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
·
2021
·Vol. 69
·期 6
·2561-2572
·
IF 2.431
Yongjun Xia,Caiyun Yang,Xiaofeng Liu,Guangqiang Wang,Zhiqiang Xiong,Xin Song,Yijin Yang,Hui Zhang,Lianzhong Ai
There have been many studies on the activities and polysaccharide production of Sanghuangporus vaninii . However, few studies have looked at triterpene production from S. vaninii using liquid-state fermentation. A method for enhancing the production of triterpenes by in situ extractive fermentation (ISEF) was studied. Eight solvents were investigated as extractants for triterpene production in the ISEF system. The results showed that using vegetable oil as an extractant significantly increased the yield of total triterpenes and biomass of S. vaninii YC-1, reaching 18.98 ± 0.71 and 44.67 ± 2.21 g/L, respectively. In 5 L fermenter experiments, the added vegetable oil improved the dissolved oxygen condition of the fermentation broth and promoted the growth of S. vaninii YC-1. Furthermore, adding vegetable oil increased the expression of fatty acid synthesis-related genes such as FAD2 and SCD , thereby increasing the synthesis of unsaturated fatty acids in the cell membrane of S. vaninii YC-1. Therefore, the cell membrane permeability of S. vaninii YC-1 increased by 19%. Our results indicated that vegetable oil increased the permeability of S. vaninii YC-1 cell membranes to promote the production of total triterpenes. The use of vegetable oil as an extractant was thus effective in increasing the yield of triterpenes in the ISEF system.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2023
·
IF 5.2
Hankui Sang,Congming Tang,Kai Ma,Xinli Li
The scarcity of fresh water resources necessitates us to develop an efficient technique for sustainable production of clean water from seawater and wastewater. Here, we constructed the 1 T-MoS 2 /PDA (polyaniline) as a photothermal material for purification of seawater and wastewater to produce clean water through interfacial evaporation by virtue of sunlight. 1 T-MoS 2 has a higher evaporation capacity of water than 2 H-MoS 2 , which was selected to prepare 1 T-MoS 2 /PDA composite for photothermal conversion. As expected, 1 T-MoS 2 /PDA composite offers a higher evaporation rate of water than both 1 T-MoS 2 and PDA , which owes to an excellent light absorbance, low light reflectance and good wettability . Under irradiation of 1 sun, the evaporation rate of water over the 1 T-MoS 2 /PDA composite in pure water reached 1.51 kg m −2 h −1 , and the photothermal conversion efficiency reached 92%. Encouragingly, 1 T-MoS 2 /PDA composite can be well used to treat real seawater and various wastewater such as organic dye solution and heavy metal ion solution, in which the evaporation rate of water is close to pure water, and the quality of water is better than the standard of drinking water established by the World Health Organization (WHO). Besides, 1 T-MoS 2 /PDA composite still maintain an excellent evaporation performance after treatment of acidic solution and alkaline solution, indicating an excellent durability. This work provides a feasible solution for producing clean water by solar-driven interfacial evaporation from seawater and wastewater.
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CATALYSIS LETTERS
·
2021
·Vol. 152
·期 9
·2832-2839
·
IF 3.186
Zhang Fengming,Li Ran,Zhang Jiapeng,Dong Hua
Dendritic fibrous KCC-1 supported CuCo bimetal catalysts (CuCo/KCC-1) are fabricated by coprecipitating Cu 2+ and Co 2+ on the surface of KCC-1 nanospheres. The microstructure and composition of the fabricated CuCo/KCC-1 bimetal catalysts are analyzed with ICP-OES, SEM, TEM, and XPS. The CuCo/KCC-1 catalysts demonstrate excellent performance in catalyzing ammonia borane hydrolysis to produce hydrogen in alkaline solutions. As an example, the hydrolysis of ammonia borane catalyzed by Cu 3 Co 1 /KCC-1 (with a Cu/Co molar ratio about 3:1) gives a hydrogen generation rate of 5.43 L/(min g metal ), and an activation energy of 23.96 kJ/mol. The synergic effect between Cu and Co in the CuCo/KCC-1 catalysts maintains the excellent catalytic activity while reducing the usage of Co, making it possible to decrease the cost of the catalyst. Graphical Abstract
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CHEMISTRY & BIODIVERSITY
·
2021
·Vol. 19
·期 2
·e202100735
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IF 2.408
Zhipeng Qi,Xianyu Fan,Chunyi Zhu,Dongsheng Chang,Jianjun Pei,Linguo Zhao
Synthesis of β-ionone in microbial cell factories is limited by the efficiency of carotenoid cleavage dioxygenases (CCDs). To obtain genes responsible for specific cleavage of carotenoids generating β-ionone, a novel carotenoid cleavage dioxygenase 1 from Morus notabilis was cloned and overexpressed in Escherichia coli . The MnCCD1 protein was able to cleave a variety of carotenoids at the positions 9, 10 (9′, 10′) to produce β-ionone, 3-hydroxy-4-oxo-β-ionone, 3-hydroxy-β-ionone, and 3-hydroxy-α-ionone in vitro . MnCCD1 could also cleave lycopene and β-carotene at the 9, 10 (9′, 10′) bind bond to produce pseudoionone and β-ionone, respectively, in E. coli accumulating carotenoids. The enzyme activity of MnCCD1 was reached 2.98 U/mL at optimized conditions (temperature 28 °C, IPTG 0.1 mM, induction time 24 h). The biochemical characterization of MnCCD1 revealed the optimal activities were at pH 8.4 and 35 °C. The addition of 10 % ethanol could increase enzyme activity at above 15 %. However, an obvious decline was observed on enzyme activity as the concentration of Fe 2+ increased (0–1 mM). The V max for β-apo-8′-carotenal was 72.5 U/mg, while the K m was 0.83 mM. The results provide a foundation for developing the application of carotenoid cleavage dioxygenases as biocatalysis and synthetic biology platforms to produce volatile aroma components from carotenoids.
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Molecular Catalysis
·
2023
·
IF 4.6
Liqiang Ma,Pengpeng Wei,Jingfang Li,Xue Lu,Guangming Li
Aerobic oxidative desulfurization (AODS) is a promising deep desulfurization technique that can efficiently remove organosulfur compounds under mild conditions. In this study, a one-pot hydrothermal method was designed to encapsulate a quaternary heteropolyacid H 4 PVMo 4 W 7 O 40 (PVMo 4 W 7 ) into rht -MOF-1 { rht -MOF-1 = [Cu 6 O(TZI) 3 (H 2 O) 6 ] 4 ⋅nH 2 O}, and the single crystal structure of H 4 PVMo 4 W 7 O 40 @ rht -MOF-1 ( 1 ) indicated that PVMo 4 W 7 was effectively encapsulated within cage C of rht -MOF-1. Further, a similar one-pot hydrothermal reaction was employed to introduce acetic acid, resulting in the synthesis of defective H 4 PVMo 4 W 7 O 40 @ rht -MOF-1-De ( 1 - De ). 1 - De was characterized using various techniques such as FT-IR, XRD, TGA, XPS, and EPR. In comparison with 1, 1 - De exhibited numerous defect sites, high porosity, high surface area, high Mo 5+ ion content, well-dispersed PVMo 4 W 7 , and abundant oxygen vacancies, leading to efficient AODS capability. When applied as catalyst, 1 - De demonstrated high catalytic activity and good recyclability for the oxidation of dibenzothiophene (DBT) and 4,6-dimethyl dibenzothiophene (4,6-DMDBT) under air atmosphere. Strikingly, the removal rates of DBT and 4,6-DMDBT reached 99 % and 96 %, respectively under optimized conditions.
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JOURNAL OF ETHNOPHARMACOLOGY
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2016
·
IF 3.055
Supawadee Parhira,Guo-Yuan Zhu,Ming Chen,Li-Ping Bai,Zhi-Hong Jiang
Ethnopharmacological relevance Calotropis gigantea (L.) Dryand (Apocynaceae) is a medicinal plant native to southern China, India and Southeast Asia. It has been traditionally used for the treatment of several diseases including cancers in these countries. Aim of the study This study aimed to isolate bioactive cardenolides from C. gigantea , to screen their hypoxia-inducible factor (HIF-) 1 inhibitory activity, and to analyze the structure-activity relationship (SAR). Materials and methods Isolation and purification of cardenolides from the latex and the fruits of C. gigantea were performed by using a series of separation techniques. Their structures were fully characterized by elucidating their NMR and HRMS data. The HIF-1 inhibitory activities of cardenolides were evaluated by using a T47D cell-based dual-luciferase reporter assay. The potent cardenolides were selected to further evaluate their dose-response manner. Cytotoxic effects of selected cardenolides were also examined against breast cancer cell line (MCF-7) and normal mammary epithelial cell line (MCF-10A) by MTT assay. Results Among twenty isolated cardenolides, compounds 1 , 3 , 4 , 6–8 , 14 and 17 exhibited stronger HIF-1 inhibitory activities than that of digoxin, a well-known HIF-1 inhibitor ( P <0.001). These eight cardenolides inhibited HIF-1 transcriptional activity in a dose-dependent manner with IC 50 values in nanomolar potency (21.8–64.9 nM). An analysis of SAR revealed the great contributions of a β -configuration of the substituents at positions of C-2′ and C-3′, an aldehydic moiety on C-19, and the dioxane moiety between the aglycone and sugar parts of cardenolides to the HIF-1 inhibitory activity. In contrast, a hydroxyl group at any positions of C-15, C-16 and C-4′ of cardenolides showed negative effects on suppressing HIF-1 transcriptional activity. In addition, these eight cardenolides also exhibited potent cytotoxic effects against human breast cancer cell MCF-7 (IC 50 values ranged from 30.5 to 68.8 nM), but less toxic effects to human normal mammary epithelial cell MCF-10A (IC 50 values >20 µM). Conclusions This is the first report of a comprehensive study of SAR on cardenolides from C. gigantea as HIF-1 inhibitors. Eight cardenolides ( 1 , 3 , 4 , 6–8 , 14 and 17 ) showed both potent HIF-1 inhibitory activity and strong cytotoxic effect against MCF-7 cancer cells in nanomolar level. The findings of these cardenolides provided important insights into the development of these potent HIF-1 inhibitors as anticancer drug .
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JOURNAL OF NUTRITIONAL BIOCHEMISTRY
·
2026
·
IF 5.2
Liangyong Shao,Xiaohan Zhang,Jiamei Xin,Baorui Li,Huiyong Lian,Jiao Xie,Kai Yu,Ye Zhang,Hao Wang
Silibinin had both anti-obesity and anti-aging effects. • The effects of Silibinin on obesity and aging were linked to the IIS pathway. • Network pharmacology and molecular docking verified Silibinin 's anti-obesity mechanism. Although silibinin (SIL), from milk thistle, has been reported to promise health promotion and disease treatment, the potential mechanism of SIL in improving obesity is still unknown. The fat accumulation was decreased by 78% and 49% in cellular and high glucose-induced (HGI) Caenorhabditis elegans ( C.elegans ) models. The decreased lipid droplets in both average size and quantity in the cytoplasm of the liver and EWATs were obtained by histology (hematoxylin & eosin) experiments. The promoted health effects in lifespan, stress ability, antioxidant ability, changed lipofuscin accumulation, pharyngeal pumping rate, and aging-related indexes of exercise were also confirmed. The increased expressions of daf-2, akt-1, daf-16, and skn-1 , combined with the downstream target genes of IIS-mediated mTOR signaling factors ( pha-4, hlh-30, lgg-1, and unc-51 ) and adipose-related factors ( nhr-49, fat-5, fat-6, and fat-7 ) in C. elegans were verified through the insulin/insulin-like growth factor-1 signaling pathway. SIL was proposed as a potential adjuvant remedy or nutraceutical for improving obesity and promoting health. Download: Download high-res image (146KB) Download: Download full-size image
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JOURNAL OF CONTROLLED RELEASE
·
2023
·
IF 10.8
Tiantian Liu,Zehang Zhou,Mengxing Zhang,Puxin Lang,Jing Li,Zhenmi Liu,Zhirong Zhang,Lin Li,Ling Zhang
The cuproptosis cell death pathway brings fresh opportunities for tumor therapy. However, efficient and targeted cuproptosis induction in tumors is still a challenge. Unfortunately, the well-known cuproptosis initiator, disulfiram and copper complex (DSF/Cu 2+ ), also increases PD-L1 level in tumors, which may diminish the final therapeutic outcome. In this study, DSF/Cu 2+ -loading MXene nanosheets are coated with PD-1 overexpressing T cell membrane to generate CuX-P system. CuX-P could recognize and stick to PD-L1 on tumor cells like a patch, which promotes the endocytosis of both CuX-P and PD-L1 by tumor cells. Following internalization and release of DSF/Cu 2+ in the cytoplasm, PD-L1 expression is upregulated. However, due to the presence of CuX-P in the tumor microenvironment , the then supplemented PD-L1 on tumor surface again binds CuX-P for internalization. This feedback loop keeps blocking and consuming the PD-L1 on tumor surface and promotes the enrichment of CuX-P in tumors to induce cuproptosis. After CuX-P treatment with laser irradiation, strong anti-tumor immune responses are stimulated in a mouse model with triple-negative breast cancer. Thus, this study develops a tumor-targeted biomimetic system that offers simultaneous cuproptosis killing, photothermal therapy (PTT) and immunotherapy in mice.
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Nanoscale
·
2023
·
IF 6.7
Yu Ran,Shanmei Yin,Pei Xie,Yaxue Liu,ying wang,Zongning Yin
Acute Lung Injury (ALI) is an inflammatory disease caused by multiple factors such as infection, trauma, and chemicals. Without effective intervention during the early stages, it usually quickly progresses to acute respiratory distress syndrome (ARDS). Since ordinary pharmaceutical preparations cannot precisely target to the lungs, their clinical application is limited. In response, we constructed a γ3 peptide-decorated and ROS-responsive nanoparticle system encapsulating therapeutic dexamethasone (Dex/PSB-γ3 NPs). In vitro, Dex/PSB-γ3 NPs had rapid H2O2 responsiveness, low cytotoxicity, and strong intracellular ROS removal capacity. In a mouse model of ALI, Dex/PSB-γ3 NPs accumulated at the injured lung rapidly, alleviating pulmonary edema and cytokine levels significantly. The modification of NPs by γ3 peptide achieved highly specific positioning of NPs in the inflammatory area. The ROS-responsive release mechanism ensured the rapid release of therapeutic dexamethasone at the inflammatory site. This combined approach improves treatment accuracy, and drug bioavailability, and effectively inhibits inflammation progression. Our study could effectively reduce the risk of ALI progressing to ARDS and hold potential for the early treatment of ALI.
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MICROPOROUS AND MESOPOROUS MATERIALS
·
2021
·
IF 5.455
Rong Pan,Yining Tang,Yanni Guo,Jun Shang,Lei Zhou,Wei Dong,Deliang He
To develop effective SO 2 adsorbents, HKUST-1 and HKUST-1 @ GO were synthesized by hydrothermal and room-temperature methods, and characterized by SEM, BET, XRD , TG and FTIR. The preparation method of the adsorbent, amount of adsorbent, initial concentration of SO 2 , and the influence of time and temperature on the adsorption of SO 2 by HKUST-1 and HKUST-1@GO were studied. The results showed that the hydrothermally synthesized HKUST-1@1.2%GO was the best adsorbent for SO 2 , the SO 2 concentration did not affect the final adsorption efficiency, the best adsorption time was 20 min, and the best adsorbent dose was 200 mg. The kinetics and isotherms of HKUST-1@1.2%GO were studied, and the thermodynamic parameters were calculated. The static SO 2 adsorption of HKUST-1@1.2%GO conformed to the pseudo-first-order model and Freundlich model, and was a spontaneous exothermic process. Lowering the temperature is beneficial to the adsorption. At 288 K, the maximum theoretical SO 2 adsorption capacity of HKUST-1 @1.2% GO was 72.49 mmol g −1 . After five adsorption cycles, the adsorption efficiency only decreased by 0.8%, indicating that the adsorbent has excellent repeatability and cyclic stability.
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MICROPOROUS AND MESOPOROUS MATERIALS
·
2021
·
IF 5.455
Xu Wang,Qing You,Yushuai Wu,Chenyao Bi,Huiyong Chen,Chengyi Dai,Qingqing Hao,Jianbo Zhang,Xiaoxun Ma
A novel hierarchical tungsten-substituted Silicalite-1 zeolite with the highly interconnected hollow structure (HWS-1) was synthesized for the first time by post-treating the purely microporous tungsten-substituted Silicalite-1 (WS-1) through simultaneous desilication and tungstation, in which a combination of sodium hydroxide and tetrapropyl ammonium hydroxide (TPAOH) was used to achieve a systematic balance between dissolution and recrystallization and further promote the synchronous tungstation with using sodium tungstate dihydrate as a tungsten source. Under an optimum addition ratio of NaOH/TPAOH, the resulted HWS-1 exhibited not only well retained microporosity and crystallinity but also obviously enlarged external surface area and mesopore volume in contrast to the parent WS-1. Furthermore, the effective tungstation also facilitated the substitution and increased the amount of coordinated W species in zeolite frameworks. The catalytic performance of HWS-1 was evaluated by the epoxidation of cyclohexene with using H 2 O 2 as oxidant. Benefited by the highly interconnected hollow structure and more accessible active sites on the enlarged external surface, the HWS-1 catalyst revealed a higher catalytic activity with improved cyclohexene conversion than WS-1. Moreover, it was found that a significant positive effect on promoting epoxidation was achieved by tungsten-substituted Silicalite-1 (WS-1 and HWS-1) catalysts reflecting at a much higher selectivity of epoxide (>79%) in comparison with titanium-substituted Silicalite-1 (TS-1) (~50% selectivity of epoxide), due to their superior hydrophobicity and suitably weaker acidity, which further demonstrated the potential application of the developed hierarchical tungsten-substituted Silicalite-1 as a candidate catalyst for epoxidation and other selective oxidation reactions.
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MOLECULAR AND CELLULAR BIOCHEMISTRY
·
2021
·Vol. 476
·期 6
·2479-2489
·
IF 3.396
Chai Qian,Meng Ziang,Lu Dexue,Zhang Ziying,Liu Meili,Wu Weihua
Cardiomyocyte death is an important pathogenic process in cardiac complications of diabetes. Diabetic patients often suffer glycemic variability. Pyroptosis is a form of programmed cell death triggered by inflammasomes and related with caspase-1 and gasdermin D activation. The present study was designed to examine the effects of intermittent high glucose simulating glycemic variability on the pyroptosis of cardiomyocytes in vitro. Rat H9C2 cardiomyocytes were incubated with normal glucose (NG), constant high glucose (CHG) and intermittent high glucose (IHG). Results showed that compared to CHG treatment, IHG further inhibited cell proliferation and promoted cell death of H9C2 cardiomyocytes. In addition, IHG upregulated higher levels of the expressions of inflammasome NLR family pyrin domain containing 3 (NLRP3) and adaptor protein apoptosis-associated speck-like protein containing CARD domain (ASC) and increased higher levels of activated caspase-1 and gasdermin D than CHG treatment. Moreover, the production of reactive oxygen species (ROS) and activation of NF-κB that is induced by IHG were significantly higher than that induced by CHG. Knockdown of sodium–glucose cotransporters 1 (SGLT1) in H9C2 cardiomyocytes was performed and the effects of SGLT1 on IHG-induced pyroptosis was evaluated. The results demonstrated that knockdown of SGLT1 partially reduced IHG-induced pyroptosis, ROS generation and NF-κB activation. Our results indicated that IHG is harmful to cardiomyocytes and it might be partially because of the SGLT1-depedent pyroptosis in cardiomyocytes.
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危险品化学品经营许可证（带存储）