ACS Nano
·
2026
·
IF 17.3
Ping Li,Qianying Li,Lu Ding,Shiyu Peng,Hui Wei,Tingtao Chen,Yonghua Xiong,Ben Zhong Tang,Xiaolin Huang
Interleukin-2 (IL-2) immunotherapy offers considerable potential for metastatic cancers; however, its efficacy is limited by low response rates, dose-dependent toxicity, short half-life, poor tumor accumulation, and off-target immune activation. In this work, we present a biohybrid microrobot (IL-2@Z/C/A) engineered through biomimetic mineralization of IL-2 variant-secreting Escherichia coli Nissle 1917 (EcN) with a zeolitic imidazolate framework-8 (ZIF-8) shell coloaded with an aggregation-induced emission photosensitizer and catalase. The ZIF-8 coating preserves bacterial viability tumor-homing capability while preventing premature drug leakage and systemic exposure, thus minimizing off-target effects. Upon accumulation in the acidic tumor microenvironment (TME), the framework degrades to release engineered bacteria and therapeutic cargo. Locally delivered zinc ion release, light-triggered reactive oxygen species and EcN derived pathogen-associated molecular patterns act synergistically to induce Cle-caspase/GSDMD mediated pyroptosis, resulting in immunogenic cell death accompanied by damage-associated molecular pattern release and pro-inflammatory cytokine production. Simultaneously, catalase-driven oxygen generation alleviates hypoxia and suppresses HIF-1α-induced immunosuppression. In combination with PD-L1 blockade, IL-2@Z/C/A achieves near-complete tumor regression in a B16F10 melanoma model via a coordinated immune cascade: pyroptosis-mediated antigen exposure primes adaptive immunity, hypoxia reversal counteracts immunosuppression, and sustained local IL-2 release reverses T cell exhaustion, collectively reprogramming the immunosuppressive TME and eliciting strong antitumor immunity. This work establishes a distinct paradigm for spatially controlled immunotherapy and highlights the potential of this biohybrid microrobot in converting immunologically “cold” tumors into responsive niches.
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PubMed
高影响因子
FUEL
·
2022
·
IF 8.035
Hua Yan,Xiaowei Liu,Junhong Zhou,Dawei Li,Xiaoye Yang,Yiming Gu,Hongjiu Su,Shudong Wang
The development of anthraquinone (AQ) hydrogenation catalysts with high activity and selectivity remains a challenge, but it is critical in industrial applications from both economic and environmental perspectives. In this work, an amine-modified catalyst, i.e., Pd/A/SiO 2 , was designed and synthesized via grafting 3-aminopropyltrimethoxysilane (APTMS) onto silica support and then loading Pd via the Shiff-base reaction. Multiple techniques were used to thoroughly characterize and analyze the catalysts' structural and electronic properties, such as transmission electron microscopy (TEM), H 2 temperature-programmed reduction (H 2 -TPR), and X-ray photoelectron spectroscopy (XPS), etc. The Pd particle size (3.86 nm) in Pd/A/SiO 2 was found to be very close to the reported optimal value, which balances well between the number of active sites and the required size for AQ adsorption; also, the residual carbon species from APTMS improves the hydrophobic properties of the support, which benefits AQ adsorption and anthrahydroquinone (AQH 2 ) desorption; and furthermore, the electron transferred from N to Pd increases the electron density of the latter, favoring the activation of the C O bond in AQ. These factors combine to give a very superior performance of Pd/A/SiO 2 for AQ hydrogenation, including a hydrogenation efficiency as high as 15.7 g/L with an intriguing selectivity of 96.1 %, and a good reusability merit within a six-run test, all of which are among the best results reported so far. This work, we believe, provides food for thought in the development of a highly efficient catalyst for AQ hydrogenation.
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高影响因子
Small
·
2023
·Vol. 19
·期 20
·2208167
·
IF 13.3
Mengya He,Xican Xu,Hongyu Wang,Qingyuan Wu,Linghao Zhang,Dongsheng Zhou,Yigang Tong,Xin Su,Huiyu Liu
Fluorescence-based PCR and other amplification methods have been used for SARS-CoV-2 diagnostics, however, it requires costly fluorescence detectors and probes limiting deploying large-scale screening. Here, a cut-price colorimetric method for SARS-CoV-2 RNA detection by iron manganese silicate nanozyme (IMSN) is established. IMSN catalyzes the oxidation of chromogenic substrates by its peroxidase (POD)-like activity, which is effectively inhibited by pyrophosphate ions (PPi). Due to the large number of PPi generated by amplification processes, SARS-CoV-2 RNA can be detected by a colorimetric readout visible to the naked eye, with the detection limit of 240 copies mL −1 . This conceptually new method has been successfully applied to correctly distinguish positive and negative oropharyngeal swab samples of COVID-19. Colorimetric assay provides a low-cost and instrumental-free solution for nucleic acid detection, which holds great potential for facilitating virus surveillance.
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高影响因子
TALANTA
·
2022
·
IF 6.556
Yaqin Zhang,Xiangyu Quan,Yingchun Li,Hangyu Guo,Fange Kong,Jiahui Lu,Lirong Teng,Jiasi Wang,Di Wang
The coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global pandemic, highlighting the unprecedented demand for rapid and portable diagnostic methods. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) proteins-based platforms have been used for the detection of pathogens. However, in further applications and research, due to multiple steps needed, many methods showed an increased risk of cross-reactivity. The thermostable Cas12b enables the combination of isothermal amplification and CRISPR-mediated detection, which could decrease the risk of cross-contamination. In this study, we developed a portable and specific diagnostic method that combined the gold nanoparticle (AuNP) with thermal stable CRISPR/Cas12b-enhanced reverse transcription loop-mediated isothermal amplification (RT-LAMP), which is called SCAN, to distinguish the N gene of SARS-CoV-2 from flu gene. We validated our method using RNA from cells transfected by plasmids. We could easily distinguish the positive results by the naked eye based on the strong molar absorption coefficient of AuNP. Moreover, SCAN has the potential for high-throughput tests owing to its convenient operation. In sum, SCAN has broken the site and equipment restrictions of traditional detection methods and could be applied outside of hospitals and clinical laboratories, greatly expanding the test of COVID-19.
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高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Lihong He,Can Zeng,Lingfeng Wei,Li Xu,Fenglin Song,Jianrong Huang,Nanjing Zhong
This study aims to fabricate immobilized lipases for efficient preparation of 1,3-dioleoyl-2-palmitoyl-glycerol (OPO) through acidolysis of glycerol tripalmitate (PPP). Twelve (three types) supports and five lipases were studied carefully. Among them, the immobilized Thermomyces lanuginosa lipase (TLL) samples exhibited overall better performance than that of other immobilized lipases. Particularly, organic groups functionalized SBA-15 (R-SBA-15) supported TLL (TLL@R-SBA-15) samples gave PPP conversion from 97.70 to 99.00 % and OPO content from 59.52 to 64.73 %. After optimization, PPP conversion up to 99.07 %, OPO content 73.15 % and sn -2 palmitic acid content 90.09 % were obtained with TLL@C 18 H 37 -SBA-15 as catalyst. Moreover, TLL@C 18 H 37 -SBA-15 exhibited better acidolysis performance from 50 °C than that from 60 to 80 °C, which helped inhibit acyl migration. In addition, after 5 cycles of reuse, TLL@C 18 H 37 -SBA-15 retained 81.04 % (based on OPO content) and 98.88 % (based on sn- 2 palmitic acid content) of its initial activity, indicating it had an attractive prospect in future applications.
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高影响因子
POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2022
·
IF 6.751
Yonghua Zhang,Qiuli OuYang,Bin Duan,Okwong Oketch Reymick,Yue Chen,Yuanzhen Tan,Xiangrong Zhu,Donglin Su,Gaoyang Li,Nengguo Tao
Green mold, caused by Penicillium digitatum , is the primary postharvest disease in citrus fruit. Trans -2-hexenal was found to exert a considerable inhibitory effect on the mycelial growth of P. digitatum with a minimum fungicidal concentration (MFC) of 0.106 g L −1 . To overcome its hydrophobic restriction, a trans -2-hexenal- β -cyclodextrin inclusion complex ( β -CDIC) was prepared by the saturated aqueous solution method. In vitro assays revealed that the MFC of β -CDIC for P. digitatum mycelial growth was 1.00 g L −1 , and 10.0 g L −1 β -CDIC apparently reduced the incidence of green mold in citrus fruit inoculated with P. digitatum while maintaining fruit quality. The structure of β -CDIC was characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), fourier transform-infrared spectroscopy (FT-IR), thermogravimetric (TG), and nuclear magnetic resonance (NMR) analyzes. The results showed that the H-1 proton of trans -2-hexenal correlated with the H-5′ proton of β -cyclodextrin ( β -CD), which was further confirmed by molecular docking analysis. The present study suggests that β -CDIC is a promising candidate for controlling postharvest diseases in citrus fruit.
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高影响因子
ACS Earth and Space Chemistry
·
2022
·
IF 3.556
Shangying Li,Hongping He,Qi Tao,Peixin Du,Aiqing Chen,Yilin He,Jianxi Zhu
2:1 type layered aluminosilicates (LAs) such as beidellite, montmorillonite, and paragonite distribute widely in the various environments of the Earth, and they form commonly through the transformation of other minerals under hydrothermal or weathering conditions. Understanding the growth mechanisms of 2:1 type LAs during mineral transformation is of great importance for profoundly revealing their formation processes in the geological environments and a better understanding of the formation of crystal defects within minerals, however, which is not thoroughly revealed. In this study, the transformation of boehmite into 2:1 type LAs was successfully realized under hydrothermal conditions, and the growth mechanisms of 2:1 type LAs during such a mineral transformation were explored using spectroscopic and microscopic characterizations. The results indicated that layers of 2:1 type LAs preferentially extend in the a–b plane at the initial transformation stage and stack along the c-axis with the transformation proceeding. Additionally, some small primary nanoparticles of 2:1 type LAs occasionally attached to each other along the c-axis or in the a–b plane. This revealed that 2:1 type LAs that formed at the interfaces between precursor boehmite and additional amorphous Si mainly grew through the layer growth mechanism occasionally accompanied by the oriented attachment.
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高影响因子
Frontiers in Pharmacology
·
2022
·
IF 5.988
Ma Quantao,Li Yaqi,Zhao Ruixue,Tang Ziyan,Li Jialin,Chen Cong,Liu Xiaoyao,Hu Yujie,Wang Ting,Zhao Baosheng
Background: Type 2 diabetes (T2D) is considered as one of the most significant metabolic syndromes worldwide, and the long-term use of the drugs already on the market for T2D often gives rise to some side effects. The mulberry leaf (ML), Morus alba L., has advantages in terms of its comprehensive therapeutic efficacy, which are characterized as multicomponent, multitarget, multipathway, and matching with the complex pathological mechanisms of diabetes.Methods: T2D rats were established by a high-fat diet combined with an intraperitoneal injection of streptozotocin; an evaluation of the hypoglycemic effects of the ML in combination with fasting blood glucose and other indicators, in addition to the utilization of metabolomics technology, was performed to analysis the metabolite changes in serum of rats.Results: MLs significantly reduced the fasting blood glucose of T2D rats, while improving the symptoms of polyphagia and polyuria. ML treatment altered the levels of various metabolites in the serum of T2D rats, which are involved in multiple metabolic pathways (amino acid metabolism, carbohydrate metabolism, and lipid metabolism), played a role in antioxidative stress and anti-inflammation, modulated immune and gluconeogenesis processes, and improved obesity as well as insulin resistance (IR).Conclusion: The ML contains a variety of chemical components, and metabolomic results have shown that MLs regulate multiple metabolic pathways to exert hypoglycemic effects, suggesting that MLs may have great promise in the development of new hypoglycemic drugs.
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高影响因子
RESEARCH ON CHEMICAL INTERMEDIATES
·
2022
·Vol. 48
·期 8
·3347-3357
·
IF 3.134
Zhang Xiangxiang,Zhou Man,Zhong Yang,Zhang Letian,Kang Jianjian,Wang Lingzhi
Co(OH) 2 modified TiO 2 (Co(OH) 2 /TiO 2 ) was fabricated by a simple precipitation method and applied as a surface enhanced Raman scattering (SERS) substrate. Using triphenylmethane dye acid blue 83 as the analyte, the SERS substrate showed high sensitivity with a detection limit of 1 × 10 –7 M and high signal reproducibility with a relative standard deviation of 8.9%. Moreover, the substrate can also be used to in situ monitor the catalytic degradation of Acid blue 83. In addition, compared with cationic triphenylmethane dye, the Co(OH) 2 /TiO 2 substrate presented stronger SERS activity towards anionic counterparts. The easy preparation and high sensitivity and selectivity towards anionic dyes of Co(OH) 2 /TiO 2 provides a robust alternative for promoting the practical application of semiconductor-based SERS substrate in selective detection.
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高影响因子
CRYSTENGCOMM
·
2022
·Vol. 24
·期 35
·6232-6238
·
IF 3.756
Duanliang Wang,Defu Sun,Shoubao Zhang,Jiancai Leng,Ruoxian Hou,Hanzhang Chen,Jie Sun,Yangyang Dang,Chuanying Shen
Organic phosphate nonlinear optical (NLO) materials, especially with the introduction of π-conjugated organic cations, have attracted attention due to their excellent performance and promising applications. Here, by combining the π-conjugated organic cationic 2-(aminomethyl)pyridine ((C6H9N2)+) with the inorganic moiety H2PO4−, a novel (C6H9N2)H2PO4 ((2-Ampy)H2PO4) crystal with a non-centrosymmetric structure has been grown by the slow evaporation method under the conditions of RT and ambient atmosphere. The data about the crystal structure, optical, thermal, photoluminescence and nonlinear optical properties were sufficiently studied. (2-Ampy)H2PO4 exhibits a high diffuse reflection with wide transparent range and appropriate thermal stability. Furthermore, an obvious photoluminescence characteristic was presented and displayed two emission peaks with positions at about 385 and 489 nm, while the average PL lifetime was 5.36 μs under the excitation of 300 nm. Importantly, SHG about 0.3× that of KDP is attributed to the hydrogen bond and π-conjugated cationic effects for (2-Ampy)H2PO4 crystal powder. By DFT calculations, the simulated band gap is about 3.84 eV, and the electronic states around the forbidden gap are largely determined by the combined effects of the (2-Ampy)+ and (H2PO4)− groups’ contribution to the optical features. All the above characteristics should be beneficial to the design and application of novel organic–inorganic hybrid phosphate NLO materials.
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高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2022
·
IF 4.601
Ying Zhang,Stanislas Nsanzamahoro,Cheng-Bo Wang,Wei-Feng Wang,Jun-Li Yang
Prolyl hydroxylase 2 (PHD2) is a key oxygen receptor regulating oxygen homeostasis in human body, and it is one of the important targets for drug research and development of hypoxia related diseases. In PHD2 enzymatic reaction, the structure of substrate (HIF-1 α 556–574 ) and product (hydroxylated HIF-1 α ) peptide only differ from one oxygen atom (MW>2000), which makes it a great challenge to separate them accurately and efficiently. In this work, the direct separation and detection of HIF-1 α and hydroxylated HIF-1 α has been firstly reported based on micellar electrokinetic chromatography (MEKC). Under optimized conditions, the intraday RSD of peak area and apparent electrophoretic mobility of hydroxylated HIF-1 α were 1.87% and 0.81% respectively, and the interday RSD were 2.01% and 1.03% respectively. The LOD and LOQ of the MEKC method were 10 µM and 50 µM respectively, and the recoveries was 98.42–105.38%. Subsequently, the feasibility and accuracy of MEKC method to screen PHD2 inhibitors were confirmed by using roxadustat, and the IC 50 (10.36 µM) and inhibitor type (competitive) were consistent with literature. Finally, the method was used to screen the PHD2 inhibitory activity of five traditional Chinese medicines (TCMs). The present work not only overcomes the difficulties of direct quantitative detection of hydroxylated HIF-1 α , but also provides technical support for exploring and discovering new drug leads for hypoxia-related diseases from complex matrix such as TCMs.
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高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2022
·
IF 10.53
Ming-Yu Lv,Shen-Hui Li,Heng Mao,Ying-Nan Feng,Ao-Shuai Zhang,Li-Hao Xu,Sen Wang,Wen-Wen Xie,Zhi-Ping Zhao
Polymeric membrane separations have been used for hydrocarbon recovery in some polyolefin plants due to the low energy consumption and easy operation, but the relatively low separation factor and hydrocarbon permeance block their ways to popularization. In this study, membranes with high propylene permeance and sufficient separation factor for propylene/nitrogen separation were prepared by direct incorporating 2-methylimidazole (MIM) into polydimethylsiloxane (PDMS), which manifested simultaneous promotion of separation factor and propylene permeance. Interestingly, MIM simultaneously acted in promoting the crosslinking process of PDMS and tetraethyl orthosilicate . A series of tests and characterizations were conducted to investigate the microstructure, morphology, thermal property, and mass transfer property of the membranes. The gas separation performance of the MIM-incorporated PDMS membrane with a separation factor of 20.2 and propylene permeance of 440.9 GPU outperformed many other propylene-selective membranes reported in the literature. Molecular dynamics simulations revealed that MIM had a preferential affinity for propylene rather than nitrogen, which was similar to PDMS, but the propylene-affinity of MIM was significantly higher than that of PDMS, accounting for the promoted separation of propylene/nitrogen upon the incorporation of MIM. The MIM-incorporated PDMS membranes with readily available, cheap materials and straightforward manufacturing procedures should be a promising alternative for hydrocarbon recovery. It is also enlightening for the study and design of membrane materials for gas separation.
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高影响因子
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