INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
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2023
·
IF 4.2
Xiaoyan Zheng,Tianhao Zhang,Jie Li,Guoliang Zhang,Hongnan Chen,Shasha Cao,Chunshan Li,Guangzhou Jin
2-Ethyl-3-oxazolidineethanol (OE) is an important pharmaceutical intermediate for the treatment of dental plaque. A systematic investigation of the one-pot synthesis of OE from propanal (PA) and diethanolamine (DEA) was conducted herein. The process was carried out under mild reaction conditions without any addition of catalyst, and the product was characterized by Fourier transform infrared spectroscopy (FT-IR), 1H nuclear magnetic resonance (NMR), and electrospray ionization-tandem mass spectrometry (ESI-MS/MS). Additionally, the effects of the PA/DEA ratio, methanol/DEA ratio, reaction time, and temperature on the reaction results were explored. Based on the single-factor experiments, response surface methodology (RSM) was used to optimize the reaction conditions and it achieved a high OE yield of 98.48%, which is close to the prediction value of 98.38%. Furthermore, the reaction kinetics were calculated utilizing the second-order kinetic model, and activation energies of the forward and reverse reactions were 31.35 and 41.85 kJ·mol–1, respectively. Under the optimization reaction conditions, OE was efficiently synthesized, indicating a significant potential for industrial applications.
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POLYMER
·
2023
·
IF 4.6
Yuhui Xie,Xue Lv,Xinyi Sui,Song Tian,Li'an Jiang,Shulin Sun
In this paper, the physicochemical double cross-linking nanocomposite hydrogels were prepared. Due to the addition of ionic liquid (1-butyl-3-methylimidazolium chloride salt), Laponite cannot spontaneously form a high thixotropic gel in water. The homogeneously dispersed Laponite nanosheets act as dynamic physical crosslinking sites, which cooperate with the chemical crosslinking center of MBA in the polymer chain segments and build a super-strong and super-tough hydrogel network structure. The PAM/Laponite/IL nanocomposite hydrogel achieves excellent tensile strength up to 1191 Kpa, a high tensile strain of 6842%, and a very high toughness of 26.62 MJ/m 3 . In this paper, an effective mechanism to enhance the mechanical properties of the hydrogel is proposed based on the structural evolution of Laponite. This highly flexible nanocomposite hydrogel shows excellent potential as a load-bearing material.
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MATERIALS RESEARCH BULLETIN
·
2023
·
IF 5.4
Jinbiao Jia,Beibei Shi,Jia Dong,Xu Feng,Yangqing Wu,Bingqiang Cao
Although perovskite solar cells have made remarkable progress, the record device efficiency still lags far behind the theoretical limit efficiency. In this work, the ionic liquid, 1-ethyl-3-methylimidazolium chloride, is introduced into the triple-cation perovskite light absorption layer. The perovskite film with optimal amount of 1-ethyl-3-methylimidazolium chloride increases crystallinity, decreases pinholes and reduces defects density. As a result, the nonradiative recombination loss is efficiently restrained, accelerating the carrier extraction. The device modified with 1-ethyl-3-methylimidazolium chloride acquires a power conversion efficiency of 21.54%, which is considerably higher than that of the control device (18.12%). This work demonstrates that 1-ethyl-3-methylimidazolium chloride is a promising ionic liquid additive for high-performance perovskite solar cells.
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Journal of Cleaner Production
·
2023
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IF 11.1
Chen Fang,Zixuan Hao,Yanlan Wang,Yingping Huang,Di Huang,Xiang Liu
Nanoreactors have been enlightened by enzymes to benefit from particular confinement for purpose of the acceleration of catalytic reactions and rendering them specific. Herein, we have first designed and synthesized the high-efficiency carbon nanotube-stabilized cobalt oxides nanoparticles (CoO x /CNT), via immobilization of CoO x nanoparticles onto carbon nanotube (CNT), for 3-aminophenol (3-AP) degradation via peroxymonosulfate (PMS) activation. Among them, carbon nanotube is used as a nanoreactor for efficient degradation of 3-AP over CoO x /CNT nanocomposite . The detailed physical characterizations illustrate that CoO x /CNT presented a nanotube-shaped structure, and some CoO x nanoparticles were encapsulated into CNT, and the molar ration of Co and O in CoO x was calculated to be 1:1.006, where CoO 1.006 might be the mixture of CoO and Co 3 O 4 . The comparison with other carriers (including MoS 2 , CeO 2 , ZnO and ZrO 2 ), CoO x /CNT provides the highest 3-AP degradation efficiency, highlighting the key role of CNT, as a nanoreactor, in CoO x /CNT for activating PMS to degrade 3-AP. The super-high catalytic activity of CoO x /CNT nanocomposite in the 3-AP degradation is owing to the specific confinement (encapsulation of some CoO x by carbon nanotubes). It seems the electron transfer from CNT to Co atom surface, enriched its surface electron cloud density, which is favorable for PMS activation. Quenching experiments and electron paramagnetic resonance (EPR) result have verified that SO 4 • - and OH• are the primary reactive oxygen species in 3-AP degradation over CoO x /CNT/PMS system. This study provides a new research orientation for the synthesis of efficient and stable carbon nanotube-encapsulated Co-based nanocatalyst for wastewater treatment .
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APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2023
·Vol. 107
·期 5
·1725-1736
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IF 5
Li Jing,Li Ziyi,Gong Hui,Ma Mengyi,Li Shuolei,Yang Huilin,Zhang Hailin,Liu Jianguo
Abstract In this study, a novel high-activity amylosucrase from Salinispirillum sp. LH10-3-1 (SaAS) was identified and characterized. The recombinant enzyme was determined as a monomer with a molecular mass of 75 kDa. SaAS protein exhibited the maximum total and polymerization activities at pH 9.0 and maximum hydrolysis activity at pH 8.0. The optimum temperature for total, polymerization, and hydrolysis activities were 40, 40, and 45 °C, respectively. Under the optimal pH and temperature, SaAS had a specific activity of 108.2 U/mg. SaAS also showed excellent salt tolerance and could retain 77.4% of its original total activity at 4.0 M NaCl. The addition of Mg 2+ , Ba 2+ , and Ca 2+ enhanced the total activity of SaAS. When the conversion of 0.1 M and 1.0 M sucrose was catalyzed at pH 9.0 and 40 °C for 24 h, the ratios of hydrolysis, polymerization, and isomerization reactions were 11.9:77.4:10.7 and 15.3:53.5:31.2, respectively. The α-arbutin yield of 60.3% was achieved from 20 mM sucrose and 5 mM hydroquinone catalyzed by SaAS. Key points
• A novel amylosucrase from Salinispirillum sp. LH10-3-1 (SaAS) was characterized.
• SaAS has the highest specific enzyme activity among all known amylosucrase.
• SaAS has hydrolysis, polymerization, isomerization, and glucosyltransferase activities.
Graphical Abstract
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Biosensors-Basel
·
2022
·Vol. 12
·期 12
·1141
·
IF 5.743
Chunyuan Tian,Minggang Wei,Xiaobin Wang,Qing Hua,Feiyan Tang,Lijun Zhao,Xuming Zhuang,Feng Luan
In the present study, the electrochemiluminescence (ECL) properties of Gd(OH)3nanocrystals with K2S2O8as the cathode coreactant were studied for the first time. Based on the prominent ECL behavior of this material and the excellent specificity of the aptamer technique, an ECL aptasensor for the detection of ochratoxin A (OTA) was formulated successfully. Over an OTA concentration range of 0.01 pg mL−1to 10 ng mL−1, the change in the ECL signal was highly linear with the OTA concentration, and the limit of detection (LOD) was 0.0027 pg mL−1. Finally, the ECL aptasensor was further used to detect OTA in real samples (grapes and corn) and satisfactory results were obtained, which indicated that the built method is expected to be applied in food detection.Keywords:Gd(OH)3nanocrystals;electrochemiluminescence;aptasensor;ochratoxin A;food detection
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BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
·
2022
·
IF 1.762
Jingjing Wang,Jinyong Wu,Zhongkui Li,Xiangsong Chen,Weiwei Liu,Jianming Yao
Protein engineering is an effective method to improve the thermal stability of biological macromolecules. Cytidine monophosphate kinase (CMK) is a key enzyme involved in nucleotide metabolism and participates in the production of cytidine triphosphate. It has previously been confirmed that CMK shows low thermal stability. This study aimed to enhance the thermal stability of CMK. Herein, we first analyzed native CMK using PoPMuSiC. Thereafter, we constructed single point mutants (Q30G, P62R, E85I, D148W and K165L) and multipoint mutants of CMK with higher thermal stability using site-directed mutagenesis. The thermal stability of the mutant Q30G/E85I/K165L was observed to be 1.2-fold higher than that of the wild type. The mutants were then allowed to catalyse 3′-sialyllactose (3′-SL) synthesis. After 51.72 mmol/L N-acetylneuraminic acid (sialic acid) was consumed in a 25 mL reaction system and 50.67 mmol/L 3′-SL was obtained, a decrease of 2 h was recorded in the reaction time, whereas the yield rate increased by 33.6%. The results suggest that the mutant Q30G/E85I/K165L can improve the thermal stability of CMK and its catalytic efficiency for 3′-SL synthesis. These findings are crucial for various applications involving enzyme conversion.
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JOURNAL OF LUMINESCENCE
·
2023
·
IF 3.6
Renping Cao,Caiping Chen,Fangrui Cheng,Ting Chen,Bang Lan,Lan Li,Ru Liu,Jing Wang
In this work, we synthesize Ca 3 Bi(PO 4 ) 3 :Dy 3+ , Ca 3 Bi(PO 4 ) 3 :Eu 3+ , and Ca 3 Bi(PO 4 ) 3 :Eu 3+ , Dy 3+ . The crystal structure, phase purity, and the luminescence properties of samples are investigated, respectively. Ca 3 Bi(PO 4 ) 3 :Dy 3+ under excitation at 350 nm shows yellow emission because of the 4 F 9/2 → 6 H 15/2 , 6 H 13/2 , 6 H 11/2 , and 6 H 9/2 transitions of Dy 3+ . Ca 3 Bi(PO 4 ) 3 :Eu 3+ with excitation at 394 nm emits red-orange emission due to the 5 D 0 → 7 F 1 , 7 F 2 , 7 F 3 , and 7 F 4 transitions of Eu 3+ ion. Ca 3 Bi(PO 4 ) 3 :Dy 3+ , Eu 3+ with excitation at 394 nm mainly shows the emission of Eu 3+ , however, that with excitation at 350 nm mainly derive from Dy 3+ ion. We investigate the effects of the ratio between Dy 3+ and Eu 3+ in Ca 3 Bi(PO 4 ) 3 :Eu 3+ , Dy 3+ on the luminescence properties. The energy transfer from Dy 3+ to Eu 3+ in Ca 3 Bi(PO 4 ) 3 :Eu 3+ , Dy 3+ is found. We analyze the luminous mechanism of samples by energy level diagrams of Dy 3+ and Eu 3+ ions. The results are helpful for the research of other Dy 3+ and Eu 3+ co-doped luminescence materials.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2022
·
IF 5.895
Chunle Wei,Xiong Yang,Shaojie Shi,Lian Bai,Deyu Hu,Runjiang Song,Baoan Song
3-Hydroxy-2-oxindole motif constitutes a core structure in numerous natural products and imparts notable biological activities. Here, we describe the design and synthesis of four series of novel 3-substituted-3-hydroxy-2-oxindole derivatives containing sulfonamide moiety along with their antiviral activities against potato virus Y (PVY). Compound 10b displayed optimal antiviral activity and superior anti-PVY activity compared with the lead compound and commercial Ningnanmycin in terms of curative and protective effects. Additionally, 10b considerably inhibited PVY systemic infection in Nicotiana benthamiana. Physiological and biochemical analyses revealed that the activities of the four crucial defense-related enzymes increased in the tobacco plant following treatment with 10b. RNA-sequencing analysis revealed that 10b substantially induced the upregulation of 38 differentially expressed genes, which were enriched in the photosynthesis pathway. These findings suggest that 10b is a promising antiviral agrochemical that can effectively control PVY infection and trigger plant host immunity to develop virus resistance. This study provides novel molecular entities and ideas for developing new pesticides.
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Food & Function
·
2023
·Vol. 14
·期 6
·2684-2697
·
IF 6.1
Ran Wei,Zhucheng Su,Gerardo G. Mackenzie
Chlorogenic acid (CGA) and epigallocatechin-3-gallate (EGCG) are major polyphenolic constituents of coffee and green tea with beneficial health properties. In this study, we evaluated the gut protecting effect of CGA and EGCG, alone or in combination, on D-galactose-induced aging mice. CGA plus EGCG more effectively improved the cognition deficits and protected the gut barrier function, compared with the agents alone. Specifically, CGA plus EGCG prevented the D-galactose mediated reactive oxygen species accumulation by increasing the total antioxidant capacity, reducing the levels of malondialdehyde, and suppressing the activity of the antioxidant enzymes superoxide dismutase and catalase. In addition, supplementation of CGA and EGCG suppressed gut inflammation by reducing the levels of the proinflammatory cytokines TNFα, IFNγ, IL-1β and IL-6. Moreover, CGA and EGCG modulated the gut microbiome altered by D-galactose. For instance, CGA plus EGCG restored the Firmicutes/Bacteroidetes ratio of the aging mice to control levels. Furthermore, CGA plus EGCG decreased the abundance of Lactobacillaceae, Erysipelotrichaceae, and Deferribacteraceae, while increased the abundance of Lachnospiraceae, Muribaculaceae, and Rikenellaceae, at the family level. In conclusion, CGA in combination with EGCG ameliorated the gut alterations induced by aging, in part, through antioxidant and anti-inflammatory effects, along with its gut microbiota modulatory capacity.
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NANOTECHNOLOGY
·
2023
·Vol. 34
·期 13
·135601
·
IF 3.5
Yudan Chai,Xuedong Wei,Yufen Wang,Shuangyan Qiao
In this paper, a Cr(OH)3 NSs@ZIF67 (NSs = nanosheets) electrocatalyst is prepared on foam Ni via a simple and rapid electrochemical deposition method. Excellent electrocatalytic activity of Cr(OH)3 NSs@ZIF67 is demonstrated. It can use the overpotential of 281 mV and 390 mV respectively to drive 10 mA cm−2 and 50 mA cm−2. It is observed that the Cr(OH)3 NSs@ZIF67 electrode has the highest initial current density at 1.57 V compared with the other two monomer electrodes and shows excellent stability at the end of 60 000 s. It has the largest electrochemical activity specific surface and lowest charge-transfer resistance, and M–O bonds (M = Co, Cr) and shifting of binding energy peaks at the interface lead to more active sites and more efficient electron transfer for oxygen evolution reaction. This work highlights the construction of highly efficient composite electrocatalysts composted of low-dimensional non-precious transition metal compounds and metalorganic frameworks, promoting the development of low-cost non-noble metal composites in energy chemistry.
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JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
·
2023
·
IF 3.6
Xiaofei Ren,Kui Xu,Jianming Xu,Qiao Mei
Melatonin possesses potent hepatoprotective properties, but it remains to be elucidated whether melatonin has a therapeutic effect on monocrotaline (MCT)-induced hepatic sinusoidal obstruction syndrome (HSOS). In this study, male Sprague Dawley rats were intraperitoneally injected with melatonin or the same volume of vehicle at 0 and 24 h after MCT intragastric administration. Next, hematoxylin–eosin staining and electron microscopy were performed to evaluate the hepatic sinusoidal injury of rats. Endothelial cell marker RECA-1 was observed by immunohistochemistry. Hepatic oxidative stress was analyzed by detecting malondialdehyde, glutathione S-transferase, and reactive oxygen species. Assessment of liver function was carried out by analysis of serum aspartate aminotransferase, alanine aminotransferase, total bilirubin, and albumin levels. Real-time polymerase chain reaction and Western blot analysis were used to identify liver Sirtuin-3 (SIRT3) and active matrix metallopeptidase 9 (MMP-9) expression. Besides, liver sinusoidal endothelial cells (LSECs) were used for the in vitro functional verification experiment. Specifically, liver histology of the melatonin-treated groups showed that the pathological damages caused by MCT were significantly attenuated, total HSOS scores were decreased, and the elevation of serum hyaluronic acid observed in the model group was also reduced. Moreover, melatonin treatment also improved the survival of rats after partial hepatectomy. Administration of melatonin ameliorated MCT-induced LSECs injury, hepatic oxidative stress, and hepatic dysfunction. Furthermore, melatonin treatment increased SIRT3 expression while attenuating MMP-9 activity in liver tissues. Cell experiment also demonstrated that SIRT3 might mediate the protective effect of melatonin on LSECs. Collectively, our study provided the potential rationale for the application of melatonin for the prevention of MCT-induced HSOS.
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