ENVIRONMENTAL SCIENCE & TECHNOLOGY
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2017
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IF 6.198
Shenyu Lan,Jinxi Feng,Ya Xiong,Shuanghong Tian,Shengwei Liu,Lingjun Kong
Piezo-catalysis was first used to degrade a nondye pollutant, 4-chlorophenol (4-CP). In this process, hydrothermally synthesized tetragonal BaTiO3 nano/micrometer-sized particles were used as the piezo-catalyst, and the ultrasonic irradiation with low frequency was selected as the vibration energy to cause the deformation of tetragonal BaTiO3. It was found that the piezoelectric potential from the deformation could not only successfully degrade 4-chlorophenol but also effectively dechlorinate it at the same time, and five kinds of dechlorinated intermediates, hydroquinone, benzoquinone, phenol, cyclohexanone, and cyclohexanol, were determined. This is the first sample of piezo-dechlorination. Although various active species, including h+, e–, •H, •OH, •O2–, 1O2, and H2O2, were generated in the piezoelectric process, it was confirmed by ESR, scavenger studies, and LC-MS that the degradation and dechlorination were mainly attributed to •OH radicals. These •OH radicals were chiefly derived from the electron reduction of O2, partly from the hole oxidation of H2O. These results indicated that the piezo-catalysis was an emerging and effective advanced oxidation technology for degradation and dechlorination of organic pollutants.
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PLACENTA
·
2024
·
IF 3
Caiqun Huang
Introduction T oxoplasma gondii is an opportunistic intracellular parasite that is a major pathogenic factor in miscarriage, especially when it occurs early in pregnancy. We have previously demonstrated that the regulation of forkhead box transcription factor (Foxp3) is associated with abortion in early pregnancy caused by excretory-secretory antigen (ESA) of strain China 1. We aimed to reveal the underlying mechanism of miscarriage caused by ESA. Methods A TLR4 −/− pregnant mouse model was successfully constructed. Pregnant mice at gestational day 5 (G5) were injected with ESA. All animals were sacrificed on G13, pregnancy outcomes were observed, and abortion rates were calculated. Placental status observed by Hematoxylin-eosin staining; gene expression was measured by IHC; flow cytometry analysis was used to determine the number and function of regulatory T cells. In EL4 cells, real-time PCR and Western blot were used to evaluate gene expression and cytokines assay. Results In vivo studies revealed that ESA injection caused 83% abortion in pregnant mice but only 35% abortion in TLR4−/− pregnant mice. In addition, ESA attenuated the number and function of regulatory T cells, further suppressed Foxp3, FOXO1 levels, and upregulated CD127 expression. TLR4 −/− mice partially reversed this inhibitory effect on regulatory T cells. Furthermore, in vitro studies revealed that ESA inhibited TLR4/NF-κB signaling pathway expression and that TLR4 agonists significantly restored the ESA-induced decrease in Foxp3. Discussion These findings suggest that ESA suppresses Foxp3 expression by blocking TLR4/NF-κB signaling, resulting in miscarriage. More importantly, the results indicated that miscarriage caused by ESA is TLR4 dependent.
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GLIA
·
2025
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IF 5.1
Tingting Yang,Zhenzhou Lin,Mingjia Yu,Yongchuan Li,Jiancong Chen,Yuanchi Liu,Kaibin Huang,Suyue Pan
Status epilepticus (SE) is a severe condition that results in uncontrollable cerebral edema and cognitive dysfunction. Recent studies suggest that the localization of aquaporin-4 (AQP4) in astrocytic endfeet plays a crucial role in regulating blood–brain water transport and cell volume control, particularly along perivascular pathways. However, the signaling mechanisms underlying AQP4 localization remain poorly understood. In this study, we utilized the genetically encoded fluorescent calcium (Ca 2+ ) indicator GCaMp6f to investigate Ca 2+ signals in astrocytic somata, processes, and endfeet during SE induction and observed enhanced Ca 2+ signals in both the somata and perivascular endfeet of astrocytes. We employed genetic knockout of TRPM4 ( Trpm4 −/− ) and glibenclamide treatment to explore the role of sulfonylurea receptor 1 transient receptor potential melastatin-4 (SUR1-TRPM4) channel in these Ca 2+ responses. Both approaches significantly suppressed the Ca 2+ signals in the astrocytic endfeet and reduced perivascular expression of the Ca 2+ -related signaling pathway sensor calmodulin (CaM). Furthermore, we found that AQP4 localization was no longer confined to the domains of astrocytic endfeet following SE. Inhibition of SUR1-TRPM4 through pharmacological blockade or gene deletion restored the subcellular localization of AQP4, reduced cerebral edema, and improved cognitive outcomes post-SE. Our findings suggest that SUR1-TRPM4 plays a pivotal role in regulating astrocytic Ca 2+ signals and mediating the aberrant expression and subcellular localization of astrocytic AQP4 along perivascular pathways. Together, these findings demonstrate a novel molecular mechanism underscoring SUR1-TRPM4 therapy in the treatment of SE characterized by dysregulated Ca 2+ signaling in astrocytic endfeet.
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ACS Applied Materials & Interfaces
·
2025
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IF 8.2
Min Zhou,Ruru Xiong,Xuanxuan Wang,Huishuang Li,Lijuan Wang,Guixia Ling,Peng Zhang
Hyperpigmentation (HP) is mainly triggered by ultraviolet irradiation or local inflammation, and the free radicals generated by these two factors are the core inducers of various skin diseases. This study proposes a multipathway and highly efficient treatment strategy for melanin deposition. A microneedle (MN) system based on hydrolyzed collagen (HC) and polyvinylpyrrolidone (PVP) was designed to precisely deliver Chlamydomonas reinhardtii (C. reinhardtii) and potassium 4-MSK (C.r&4-MSK-MN) to skin tissues. C. reinhardtii contains unique antioxidant components such as arsenoproteins, polyunsaturated fatty acids (PUFAs), and algal polysaccharides, which distinguish it from other microalgae like Chlorella. In vitro free radical scavenging experiments show that at a concentration of 1 × 109 cells/mL, C. reinhardtii exhibits scavenging rates of 89.26%, 101.44%, and 103.57% against DPPH·, ABTS·+, and ·OH radicals, respectively. In addition, potassium 4-methoxysalicylate (4-MSK) can inhibit tyrosinase activity and promote skin metabolism. Therefore, C.r&4-MSK-MN has the following advantages: on one hand, C.r&4-MSK-MN successfully breaks through the skin barrier, directly delivering C. reinhardtii cells to the target site to scavenge free radicals and reduce the stimuli for melanin production. On the other hand, 4-MSK inhibits tyrosinase activity to reduce melanin synthesis and, at the same time, promotes skin metabolism to reduce melanin deposition on the skin surface, achieving multipathway reduction of melanin deposition. Experimental results showed that in animal models, C.r&4-MSK-MN significantly reduces the epidermal thickness of rats and substantially decreases melanin production. This research outcome not only provides a new strategy for the treatment of melanin deposition but also confirms that microalgae have potential application prospects in the fields of skin care (such as whitening and antiaging) and cosmeceuticals.
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CHEMICAL ENGINEERING JOURNAL
·
2025
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IF 13.2
Yulong Luo,Kerun Li,Wei Liu
The Advanced Oxidation Process (AOPs) has garnered significant attention as an efficient and promising technology for wastewater treatment in the field of environmental remediation. Currently, the exploration of highly effective catalysts for peroxymonosulfate (PMS) activation remains a focal point of scientific research, with increasing emphasis placed on the reuse of waste materials. In line with this objective, this study reports the synthesis of a Fe Co bimetallic metal-organic framework (MOF)-sponge composite (FCS) via an in-situ growth method using discarded natural sponge as a support material. Subsequently, the carbonized derivative (FCSC), obtained through high-temperature carbonization, was employed as a catalyst for PMS activation. The resulting catalyst demonstrated remarkable efficiency in removing 4-nitrophenol (4-NP) from aqueous solutions. Utilizing waste natural sponge as a support not only facilitates resource recovery but also enables the uniform distribution of MOF particles within its inherent three-dimensional porous structure. This feature enhances the contact between the catalyst and pollutants, thereby improving catalytic performance. Moreover, diatomic active sites exhibit significantly higher degradation efficiency toward 4-NP compared to the monometallic configurations. This enhanced performance can be attributed to the more rapid generation of free radicals by diatomic structures during PMS activation, which accelerates electron transfer between metal centers and adjacent oxygen atoms. This work offers novel insights into the development of efficient strategies for the removal of organic contaminants.
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MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 11
·1-11
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IF 5.3
Zhu Guancheng,Zou Jiahui,Lin Xueer,Zhong Huimin,Jiang Cong,Huang Yanyan
By self-assembly of MnCl 2 and arginine under alkaline conditions, ultra-small MnArg nanoparticles were successfully constructed as oxidase (OXD) mimics for intelligent detection of the Ginkgo toxin 4′- O -methylpyridoxal (MPN). The obtained MnArg nanozymes possessed excellent OXD-like activity and thermal stability. Based on the inhibitory effect of MPN for the catalytic activity of MnArg, this system was utilized for the colorimetric sensing of MPN with a low limit of detection (LOD) of 2.16 µg mL −1 . The detection system exhibited good selectivity against other potential interferents. FTIR data showed that the presence of MPN binds with MnArg and shields the active sites, thereby interfering with the oxidase-like activity. Combined with a smartphone and the ColorMax software, this nanozyme-based intelligent detection platform could effectively detect MPN with a LOD of 2.1 µg mL −1 . Our MnArg nanozyme–based system was applied to detect real ginkgo nut samples with recoveries of 92.4–108.7%, and the relative standard deviations were less than 0.7%. This work may promote the development of novel nanozymes and expand their applications in the field of food safety detection. Graphical Abstract
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Insects
·
2025
·Vol. 16
·期 4
·403
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IF 2.9
Xinyu Ma,Junjie Yan,Guangyuan Su,Fathiya M Khamis,Athanase Hategekiman,Yulin Gao
The potato tuber moth ( Phthorimaea operculella ) is a destructive pest that infests potato crops by boring into and eating the tubers. Although chemical pesticides are frequently employed to manage these moths, their efficacy is inconsistent and they can cause environmental damage. In this study, we investigated a natural alternative by examining specific volatiles emitted by potato plants that may repel the moths and inhibit oviposition. We identified five plant-based compounds, one of which, 4-ethylacetophenone, exhibited strong repellent effects against the moths at all tested concentrations and significantly reduced egg-laying behavior. This finding suggests that 4-ethylacetophenone could serve as an eco-friendly way to protect potato crops from P. operculella , providing a safer alternative to conventional pest control strategies. The potato tuber moth, Phthorimaea operculella (Zeller), is an important pest of potato crops capable of boring into and consuming the potato tubers. Although chemical insecticides are employed to control P. operculella populations, their efficacy is frequently limited, raising environmental concerns. Host plant volatiles are important allelochemicals that can be exploited for the management of pests. In our previous studies, we identified five volatile organic compounds (3-ethylacetophenone, 4-ethylacetophenone, geranylacetone, ethylbenzene, and 4-hydroxy-4-methyl-2-pentanone) as allelochemicals potentially influencing the oviposition behavior of P. operculella . Using electroantennography (EAG) and behavioral assays, we found that 4-ethylacetophenone significantly repelled P. operculella at all tested concentrations (1 μg, 10 μg, and 100 μg; p < 0.05), while 3-ethylacetophenone showed no significant effects ( p > 0.05). Finally, the effect of 4-ethylacetophenone on preventing P. operculella from laying eggs was determined by an oviposition selection experiment. Our findings indicate that 4-ethylacetophenone derived from potato plants not only repels P. operculella and prevents egg-laying but also has the potential to reduce the number of eggs, making it a promising green alternative for controlling this pest.
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RSC Advances
·
2025
·Vol. 15
·期 26
·21133
·
IF 4.6
Jing Chen,Xi Chen,Jinsong Song,Hongliu Zhang,Hongjiang Yang,Jinhui Feng,Qiaqing Wu,Dunming Zhu
Chiral 3-substituted-4-hydroxypiperidines are highly valuable in pharmaceutical applications due to their diverse and potent biological activities. Biocatalytic ketone reduction by carbonyl reductases represents a promising approach for synthesizing these compounds. In this study, two structurally similar yet stereoselectively distinct carbonyl reductases, HeCR and DbCR, were identified. Both enzymes exhibited exceptional catalytic performance, with >99% enantiomeric excess (ee) and >99% conversion rate in the reduction of tert -butyl 4-oxo-3-phenylpiperidine-1-carboxylate (1a). We found that 1a exhibits a relatively low rate of racemization under the mild reaction conditions. Subsequently, analogs of 1a were synthesized and reduced with high enantioselectivity (ee > 99%) using HeCR and DbCR. Carbonyl reductases demonstrated excellent catalytic activity and stereoselectivity in the synthesis of 3-substituted-4-hydroxypiperidines with dual chiral centers, underscoring their potential for pharmaceutical applications. Chiral 3-substituted-4-hydroxypiperidines are highly valuable in pharmaceutical applications due to their diverse and potent biological activities.
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Frontiers in Endocrinology
·
2025
·
IF 4.6
Emmanuel Osei Nkansah,Yunzhu Lan,Hui Zhang,Binbin Xu,Qiaodan Li,Mohammad Ishraq Zafar,Jian Xu
Background: Obesity is associated with hormonal imbalance, increased oxidative stress, and inflammation in the testis. These conditions adversely affect sperm quality, leading to impaired male fertility. Therefore, therapeutic interventions to counteract the adverse effects of obesity are crucial. This study explored the therapeutic effects of 4-hydroxyisoleucine (4-HIL) on fertility in male mice with diet-induced obesity.Methods: C57BL6 male mice (n=45) were randomly divided into normal diet (ND, n=15) and high-fat diet (HFD, n=30) groups for 10 weeks. The HFD group was then randomized into untreated (HFD, n=15) and 4-HIL-treated (200 mg/kg/day, intraperitoneal injection, HFD + 4-HIL group, n=15) for 6 weeks. ND and HFD controls received saline (0.3 mL/30 g body weight) throughout the intervention period. Comprehensive evaluations included (1) metabolic assessments (body weight, glucose, insulin and pyruvate tolerance tests, and blood serum lipids), (2) sperm analysis (count, concentration, and morphology), (3) fertility testing (mating trials and in vitro fertilization), (4) testicular histopathology (fat deposition and apoptosis), (5) biochemical assays (reproductive hormones, oxidative stress markers, and inflammatory cytokines), and (6) molecular analyses (mRNA sequencing and qPCR validation of differentially expressed genes).Results: 4-HIL treatment improved metabolic parameters, including reduced weight gain, enhanced glucose tolerance, and optimized blood serum lipids, compared to HFD controls. Treated mice exhibited superior sperm quality with increased count and concentration, reduced histomorphological abnormalities in the testis, and attenuated oxidative stress and inflammation. Furthermore, the key spermatogenic gene expressions, including spem1 and spata24, were significantly optimized in the testes of mice treated with 4-HIL compared to those of untreated mice (HFD group).Conclusion: This study demonstrates that 4-HIL therapy ameliorates obesity-induced testicular dysfunction and improves fertility markers in mice. The beneficial effects of this compound on metabolic parameters, sperm quality, and spermatogenic gene expression suggest its potential as a therapeutic agent for obesity-related male infertility. Further research is warranted to elucidate the underlying mechanisms and assess the clinical translatability of these findings.
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EUROPEAN JOURNAL OF PHARMACOLOGY
·
2020
·
IF 3.263
Bi-Yun Lin,Wen-Lin Liu,Hui Huang,Ya-Guang Hu,Sha Gong,Yu-Hua Meng,Jun Yan,Yuan-Zhi Lu,Hua-Lin Chen
The important physiological function of microtubules makes them an indispensable and clinically effective target of anti-tumor agents. Herein, we sought to design, synthesize, and evaluate a novel 4-anilinoquinazoline derivative and identify its anti-tumor activity in vitro and in vivo. The novel compound, N -(4-methoxyphenyl)- N -methyl-2-(methyl- d 3 )quinazolin-4-amine ( AQ-4 ), was identified as a representative scaffold and potent microtubule-targeting agent. As a promising antimitotic agent , AQ-4 displayed remarkable anti-tumor activity with an average IC 50 value of 19 nM across a panel of 14 human cancer cell lines. AQ-4 also exhibited nearly identical potent activities against drug-resistant cells, with no evidence of toxicity towards normal cells. A further target verification study revealed that AQ-4 targets the tubulin-microtubule system by significantly inhibiting tubulin polymerization and disrupting the intracellular microtubule spindle dynamics. According to the results of mechanism study, AQ-4 induced cell cycle arrest in the G 2 /M phase, promoting evident apoptosis and a collapses of mitochondrial membrane potential . The superior anti-tumor effect of AQ-4 in vivo suggests that it should be further investigated to validate its use for cancer therapy.
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EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
·
2023
·
IF 6.7
Yue Wu,Jin-Hui Shi,Guang-Hao Zhu,Yuan Xiong,Jia-Hao Gong,Hui-Zhen Wei,Zhao-Bin Guo,Zi-Ru Dai,Xiao-Bo Sun,Guang-Bo Ge
Human cytochrome P450 1B1 (hCYP1B1), an extrahepatic cytochrome P450 enzyme over-expressed in various tumors, has been validated as a promising target for preventing and treating cancers. Herein, two series of chalcone derivatives were synthesized to discover potent hCYP1B1 inhibitors without AhR agonist effect. Structure-activity relationship (SAR) studies demonstrated that 4′-trifluoromethyl on the B-ring strongly enhanced the anti-hCYP1B1 effects, identifying A9 as a promising lead compound. Further SAR analysis on A9 derivatives (modified A-ring of 4′-trifluoromethylchalcone) showed that introducing 2-methoxyl improved the anti-hCYP1B1 effect and selectivity , while introducing a methoxyl at the C-4 site was beneficial for avoiding AhR activation. Ultimately, five 4′-trifluoromethyl chalcones were identified as potent hCYP1B1 inhibitors (IC 50 < 10 nM), while B18 exhibits the most potent anti-hCYP1B1 effect (IC 50 = 3.6 nM), suitable metabolic stability and good cell-permeability. B18 also acted as an AhR antagonist and could down-regulate hCYP1B1 in living systems. Mechanistic studies showed that B18 potently inhibited hCYP1B1 in a competitive inhibition manner ( K i = 3.92 nM), while docking simulations revealed that B18 could tightly bind to the catalytic cavity of hCYP1B1 mainly via hydrophobic and hydrogen-bonding interactions. Furthermore, B18 could potently inhibit hCYP1B1 in living cells and showed remarkable anti-migration ability on MFC-7 cells. Taken together, this study deciphered the SARs of chalcones as hCYP1B1 inhibitors and provided several potent hCYP1B1 inhibitors as promising candidates for the development of more efficacious anti-migration agents.
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JOURNAL OF ALLOYS AND COMPOUNDS
·
2024
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IF 5.8
Bing Wang,Yao Liu,Zhuo Li,Hong Hao,YuZhen Zhao,ZeMin He,WenQi Song,EnZhou Liu,Bo Zhou,XiaoXun Ma,Zongcheng Miao
Leveraging the synergistic combination of dipole modulation and localized surface plasmon resonance effects (LSPR) effects offers a promising solution to address the low carrier separation efficiency observed at traditional Type-III heterojunction interfaces. Herein, a derivative of SubPc-4 with a high dipole moment of 7.25 Debye was successfully synthesized by introducing carbonyl functional groups axially into the parent subphthalocyanine. Silver metal was introduced during the preparation of Ag 3 PO 4 through light irradiation, and the organic supramolecule SubPc-4 self-assembled on the Ag 3 PO 4 semiconductor surface via van der Waals forces and π-π stacking interactions, forming a binary heterojunction SubPc-4/Ag 3 PO 4 with LSPR effects. Under 30 minutes of visible light irradiation, the degradation efficiencies of tetracycline and oxytetracycline by the SubPc-4/Ag 3 PO 4 supramolecular nanoarray were 88.29% and 90.20%, respectively. DFT and TDDFT calculations, combined with experimental characterization data, indicate that the LSPR supramolecular array exhibits excellent activity and stability, primarily attributed to the improved carrier separation capability at the SubPc-4/Ag 3 PO 4 interface. This work highlights the potential of combining dipole modulation and LSPR technology to enhance the performance of Type-III heterojunction photocatalysts.
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