JOURNAL OF FLUORESCENCE
·
2013
·Vol. 24
·期 2
·533-539
·
IF 1.789
Liu Chengxuan,Liang Gaiting,Liu Zhen,Zu Lily
Time-resolved fluorescence anisotropy spectroscopy was applied to study the interaction between a peptide truncated from the binding site of tumor suppressor p53 protein and the DNAs covalently labeled with 6-carboxyfluorescein (FAM) dye. Fluorescence intensity quenching and changes of anisotropy decay lifetime were monitored when FAM labeled DNA formed complex with the peptide. The results demonstrated that the sequence of DNA could not define the binding specificity between the peptide and DNA. But the anisotropy decay of FAM can be used to examine the binding affinity of the peptide to DNA. The fluorescent dynamics of FAM can also be used to represent the rigidity of the complex formed between the peptide and DNA.
研究领域:
生物化学
·
分子生物学
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DOI
PubMed
高影响因子
PROTOPLASMA
·
2018
·Vol. 255
·期 4
·1039-1052
·
IF 2.457
Li Chao,Li Cheng,Wang Bingbing,Zhang Runqi,Fu Kaiyong,Gale William J.,Li Chunyan
Drought frequently occurs during wheat ( Triticum aestivum L.) grain filling. The objectives of this study were (i) to investigate the effect of post-anthesis drought on programmed cell death (PCD) in wheat endosperm cells and (ii) to examine the role of ethylene (ETH) receptors and abscisic acid (ABA) in regulating wheat endosperm PCD. Two winter wheat cultivars (‘Xindong 18’ and ‘Xindong 22’) were used in this study. Grain samples were collected from normal and drought stressed plants at 5-day intervals between 5 and 35 days post-anthesis. The samples were then compared with respect to cell viability, nuclear morphometry, cell ultrastructure, DNA integrity, nucleic acid content, and nuclease activity. Analysis was also conducted about gene transcripts related to PCD, ETH receptors, and ABA biosynthesis and degradation. Drought stress reduced cell viability, accelerated nuclear deformation, and increased mitochondrial dissolution. The activity of nucleic acid hydrolase was greater, and the nucleic acid concentrations were less in the drought treatments than in the control. As a result, the peak in DNA fragmentation occurred earlier in the drought treatment. Drought stress significantly increased the expression of four genes related to ABA ( nced1 , nced2 , ao1 , ao2 ) . In contrast, drought significantly reduced the expression of four genes related to ETH receptors ( ers1 , ers2 etr1 , etr2 ) and one gene related to PCD ( dad1 ). In summary, the results indicated that drought stress caused PCD to occur earlier in the endosperm of winter wheat.
研究领域:
植物学
·
分子生物学
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DOI
PubMed
高影响因子
Cancer Cell International
·
2022
·Vol. 22
·期 1
·1-18
·
IF 6.429
Wang Shunxi,Chen Long,Liu Wanqian
Background Ferroptosis have been implicated in tumorigenesis, tumor progression, and chemo- and immuno-therapy in cirrhotic hepatocellular carcinoma (HCC), indicating its association with matrix stiffness and clinical benefit of targeting drugs or immune checkpoint inhibitor. Here, we postulated that increased matrix stiffness reduces ferroptosis and impairs tumor immunity by regulating the expression of ferroptosis- and immune-related genes in HCC, which might be a robust predictor of therapeutic efficacy. Methods Using publicly available tissue microarray datasets, liver cancer rat model, and clinical specimen, ferroptosis-related differential genes in HCV-infected cirrhotic HCC and its mechanical heterogeneous pattern of expression were screened and identified. Further investigation on the underlying mechanism of matrix stiffness-regulated ferroptosis and the expression of immune mediator were performed. Finally, threshold analysis of HCC cases with sorafenib treatment revealed the value of clinical applications of these potential predictors. Results STEAP3 was identified as the ferroptosis-related differential genes in HCV-infected cirrhotic HCC. Stiffer matrix decreased STEAP3 in the invasive front area of HCC and the liver cirrhotic tissue. Contrarily, softer matrix induced STEAP3 in the central area of HCC and the normal liver tissue. Immunological correlation of STEAP3 in cirrhotic HCC showed that STEAP3-mediated immune infiltration of CD4+ T and CD8+ T cells, macrophages, neutrophils, and dendritic cells and HCC prognosis, predicting to regulate immune infiltration. Overexpression of STEAP3 induced ferroptosis and inhibited the expression of immune mediator of PD-L2 on a stiff matrix. Especially, the ferroptosis- and immune-related gene predictive biomarker (FIGPB), including STEAP3 and PD-L2, predicts better clinical benefit of sorafenib in HCC patients. Conclusions This finding identifies matrix stiffness impairs ferroptosis and anti-tumor immunity by mediating STEAP3 and PD-L2. More importantly, coordinated with PD-L2, matrix stiffness-dependent STEAP3 could be applied as the independent predictors to favorable sorafenib response, and thus targeting it could be a potential diagnosis and treatment strategy for HCC.
研究领域:
肿瘤学
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免疫学
·
分子生物学
·
药理学
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DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2016
·Vol. 28
·期 5
·4088-4094
·
IF 1.798
Mao Xin,Guo Wenfeng,Li Chengzhang,Yang Jie,Du Lirong,Tang Xianzhong
All-organic polyimide (PI)/poly(vinylidene fluoride) (PVDF) composite materials with high dielectric constant and low dielectric loss were fabricated via solution blending. The dielectric, mechanical, and thermal properties of the PI/PVDF composite films were studied. Results indicated that the dielectric properties of the composites were highly reinforced through the introduction of PVDF, and the composites exhibited excellent thermal stability. When the mass fraction of PVDF was adjusted to 30 wt%, the specimen demonstrated excellent thermal properties, superior mechanical properties, high dielectric constant (5.7, 1 kHz), and low dielectric loss (0.009, 1 kHz). Moreover, the dependence of the dielectric constant and dielectric loss on frequency was investigated. The composite presented stable dielectric constant and dielectric loss that were less than 0.04 within the testing frequency range of 100 Hz–10 MHz. This study demonstrated that the PI/PVDF composites were potential dielectric materials in the field of electronics.
研究领域:
材料科学
·
电子工程
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DOI
高影响因子
CELLULOSE
·
2020
·Vol. 27
·期 7
·3611-3622
·
IF 4.21
Zhao Yang,Jin Bo,Peng Rufang,Ding Ling
The safe storage of nitrocellulose has become challenging due to their requirements of extreme storage environments, and existing stabilizers cannot fully meet the demand. Thus, developing new high-performance stabilizers to improve the stability of nitrocellulose is urgently needed. Considering the stabilizing mechanism of stabilizers and the excellent free radical scavenging ability of fullerenes, two novel fullerene-based stabilizers, pentaacyloxyphenyl fullerene derivatives (PAOP-C 60 ), were designed and synthesized, and their structures were characterized by FTIR, UV–Vis and NMR. The thermal action of PAOP-C 60 during thermal decomposition process of nitrocellulose was studied by thermal analysis, which indicating that the thermal stability of nitrocellulose was increased with addition of PAOP-C 60 and the PAOP-C 60 was found to exhibit superior thermal stability than traditional stabilizer. The results of electron spin resonance showed that fullerene-based stabilizers had a significant scavenging effect on nitrogen oxide radicals, and the IC50 of PAOP-C 60 (0.674–0.818 g L −1 ) was smaller than that of diphenylamine (1.717 g L −1 ). Moreover, the intermediate product produced by PAOP-C 60 and nitrocellulose action was extracted and characterized by FTIR. A possible stabilization mechanism of PAOP-C 60 , which was different from traditional stabilization mechanism, was proposed.
研究领域:
材料科学
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化学工程
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DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2020
·Vol. 56
·期 1
·913-926
·
IF 3.553
Du Zoufei,Liu Fei,Xiao Changfa,Dan Yi,Jiang Long
Developing a reusable and durable catalyst for heterogeneous photo-Fenton process is of great importance for the practical application in the environment remediation. In this study, poly(vinyl alcohol) (PVA)/sodium alginate (SA) hydrogel beads were constructed using FeCl 3 and boric acid as cross-linking agents. Characterizations and tests were employed to investigate the structure, optical property and durability of the obtained beads. The photo-Fenton performance of the PVA/SA-FeCl 3 beads was evaluated by degradation of tetracycline (TC) under visible-light irradiation. The results show that the constructed beads exhibit porous structure, display a broad absorption band in the visible region and behave excellent stability against mechanic effects. The photocatalytic experiment result indicates that the removal rate of TC reaches 90.5% under optimized conditions, while the removal rate of total organic carbon reaches 28.6%. By analyzing the degradation of TC after adding active species scavengers, a photocatalytic mechanism was proposed. Moreover, PVA/SA-6% FeCl 3 beads could be easily separated and recycled in water purification. This work provides a facile and robust method to construct reusable and durable Fe(III)-containing hydrogel beads with acceptable photo-Fenton catalytic performance under visible-light irradiation, paving the way for its practical application in wastewater treatment.
研究领域:
环境科学
·
材料科学
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DOI
高影响因子
MONATSHEFTE FUR CHEMIE
·
2015
·Vol. 146
·期 8
·1343-1347
·
IF 1.222
Qiu Dezhi,Wang Yanyan,Lu Dongming,Zhou Lihong,Zeng Qingle
Abstract We have developed a KOH-promoted transition-metal-free synthesis of 2-substituted 4(3 H )-quinazolinones from anthranilamides and benzyl alcohols or cinnamyl alcohol with air as oxidant. The protocol is simple, practical, and cost saving. Graphical abstract
研究领域:
有机化学
·
药物化学
✓ 已验证
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2020
·Vol. 412
·期 19
·4629-4638
·
IF 3.637
Munyemana Jean Claude,Chen Jia,Wei Xin,Ali Mohammad Chand,Han Yangxia,Qiu Hongdeng
In the quest for alternative products that would conquer natural enzyme drawbacks, enzyme-like nanomaterials with controllable morphology, high catalytic activity, excellent stability, and reusability have gained extensive attention in recent years. Herein, a simple and versatile strategy based on basic deep eutectic solvents was used to create layered copper hydroxide nitrate (Cu 2 (OH) 3 NO 3 ) with a well-structured nanosheet-like morphology. The present nanosheets exhibited extraordinary oxidase and peroxidase-like activity. More importantly, these nanosheets have shown the ability to operate at low and high temperatures with appreciable stability and multiple reusabilities. Based on inhibiting the oxidase-like activity of the prepared Cu 2 (OH) 3 NO 3 , we designed a colorimetric sensing technique with a high-efficiency detection of biothiols in serum samples. Because of the simplicity and low-cost fabrication approach, our findings would be beneficial to the artificial enzyme research community as another facile and green tactics to fabricate heterogeneous artificial enzymes.
Graphical abstract
研究领域:
材料科学
·
生物化学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2021
·Vol. 19
·期 1
·1-16
·
IF 10.435
Zhao Jiayan,Wu Hang,Zhao Jiulong,Yin Yichen,Zhang Zhilun,Wang Shige,Lin Kun
Owing to the hypoxia status of the tumor, the reactive oxygen species (ROS) production during photodynamic therapy (PDT) of the tumor is less efficient. Herein, a facile method which involves the synthesis of Mg–Mn–Al layered double hydroxides (LDH) clay with MoS2 doping in the surface and anionic layer space of LDH was presented, to integrate the photo-thermal effect of MoS2 and imaging and catalytic functions of Mg–Mn–Al LDH. The designed LDH-MoS2 (LMM) clay composite was further surface-coated with bovine serum albumin (BSA) to maintain the colloidal stability of LMM in physiological environment. A photosensitizer, chlorin e6 (Ce6), was absorbed at the surface and anionic layer space of LMM@BSA. In the LMM formulation, the magnetic resonance imaging of Mg–Mn–Al LDH was enhanced thanks to the reduced and acid microenvironment of the tumor. Notably, the ROS production and PDT efficiency of Ce6 were significantly improved, because LMM@BSA could catalyze the decomposing of the overexpressed H2O2 in tumors to produce oxygen. The biocompatible LMM@BSA that played the synergism with tumor microenvironment is a promising candidate for the effective treatment of cancer.
研究领域:
生物医学工程
·
纳米医学
·
肿瘤治疗学
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DOI
PubMed
高影响因子
Science China-Chemistry
·
2012
·Vol. 55
·期 5
·772-778
·
IF 1.02
Qin AnJun,Zhang Ya,Han Ning,Mei Ju,Sun JingZhi,Fan WeiMin,Tang Ben Zhong
An amphiphilic polymer bearing tetraphenylethene (TPE) moiety was synthesized by convenient reactions. The polymer exhibits unique aggregation-induced emission (AIE) characteristics and can self-assemble to size-tunable particles in DMF/water mixtures. The polymer nanoparticles can be used for cell imaging, which provides a potential stable fluorescent tool to monitor the distribution of drugs and bioconjugates in living cells.
✓ 已验证
DOI
高影响因子
CHINESE JOURNAL OF POLYMER SCIENCE
·
2013
·Vol. 31
·期 3
·530-540
·
IF 1.27
Li Nan,Jin Yue,Xue Li-zhe,Li Pei-yong,Yan De-yue,Zhu Xin-yuan
Hyperbranched polysulfonamine (HPSA) is a promising biomaterial due to its highly branched spherical architecture and efficient intracellular translocation. To realize the functionalization of HPSA, both N -succinimidyl 3-(2-pyridyldithio) propionate (SPDP) for tethering the human-mouse chimeric monoclonal antibody CH12 and N -hydroxy succinimidyl S -acetylmercaptoacetyltriglycinate (NHS-MAG 3 ) for labeling 188 Re were sequentially grafted onto the primary amine terminals of HPSA via covalent linkages, attaining the SPDP-HPSA-MAG 3 intermediate. In order to reserve the structural integrity of CH12, the fragment crystallizable (Fc) region was also processed by oxidation of oligosaccharide moieties with sodium periodate and then reacted with N -( κ -maleimidoundecanoic acid) hydrazide (KMUH). After chelating 188 Re with MAG 3 group, the SPDP was reduced to PDP and connected onto the maleinimide group at the Fc region. As a result, both the epidermal growth factor receptor vIII (EGFRvIII) targeted monoclonal antibody CH12 and the radionuclide 188 Re were conjugated to the HPSA-based vehicles, forming the 188 Re-labeled and CH12-tethered HPSA (CH12-HPSA- 188 Re). The molecular weight and in vitro stability of CH12-HPSA- 188 Re were evaluated by gel electrophoresis and paper chromatography. On one hand, the CH12-HPSA- 188 Re could specifically bind to the EGFRvIII-positive human hepatocarcinoma cells in vitro . On the other hand, it could also target at the tumor tissue of nude mice in vivo . Hence, the CH12-HPSA- 188 Re could effectively target at the human hepatocarcinoma and facilitate the tumor detection and targeted radioimmunotherapy.
研究领域:
医学
·
生物化学
·
分子生物学
·
肿瘤学
✓ 已验证
DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
·
2012
·Vol. 23
·期 6
·1499-1510
·
IF 2.316
Wang Heyun,Feng Yakai,An Bo,Zhang Wencheng,Sun Minglin,Fang Zichen,Yuan Wenjie,Khan Massuri
Poly(ethylene glycol) methacrylate (PEGMA) was introduced into a polyurethane (PU) solution in order to prepare electrospun scaffold with improving the biocompatibility by electrospinning technology for potential application as small diameter vascular scaffolds. Crosslinked electrospun PU/PEGMA hybrid nanofibers were fabricated by a reactive electrospinning process with N , N ′-methylenebisacrylamide as crosslinker and benzophenone as photoinitiator. The photoinduced polymerization and crosslinking reaction took place simultaneously during the electrospinning process. The electrospinning solutions with various weight ratios of PU/PEGMA were successfully electrospun. No significant difference in the scaffold morphology was found by SEM when PEGMA content was <20 wt%. The crosslinked fibrous scaffolds of PU/PEGMA exhibited higher mechanical strength than the pure PU scaffold. The hydrophilicity of scaffolds was controlled by varying the PU/PEGMA weight ratio. The tissue compatibility of electrospun nanofibrous scaffolds were tested using human umbilical vein endothelial cells (HUVECs). Cell morphology and cell proliferation were measured by SEM, fluorescence microscopy and thiazolyl blue assay (MTT) after 1, 3, 7 days of culture. The results indicated that the cell morphology and proliferation on the crosslinked PU/PEGMA scaffolds were better than that on the pure PU scaffold. Furthermore, the appropriate hydrophilic surface with water contact angle in the range of 55–75° was favorable of improvement the HUVECs adhesion and proliferation. Cells seeded on the crosslinked PU/PEGMA (80/20) scaffolds infiltrated into the scaffolds after 7 days of growth. These results indicated the crosslinked electrospun PU/PEGMA nanofibrous scaffolds were potential substitutes for artificial vascular scaffolds.
研究领域:
生物材料学
·
组织工程学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）