Foods
·
2023
·Vol. 12
·期 21
·4005
·
IF 5.2
He Wang,Yue Zheng,Xinyue Tang,Ting Zhang
Loquat is a nutrient-rich fruit with juicy and sweet pulp, but it is vulnerable to rot and deterioration without proper postharvest preservation measures. This study aimed to improve the postharvest quality of loquat by developing a microemulsion system based on an essential oil extracted from theTorreya grandiscv. Merrillii aril (TaEO), which has antimicrobial and antioxidant properties. An optimal TaEO microemulsion (TaEO-ME) was formulated, using a mixture of Tween-40 and Tween-80 as the surfactant, 1-butanol as the co-surfactant, and TaEO as the oil phase, with mass ratios of 9:1, 3:1, and 6:1, respectively. Two TaEO-ME formulations with 60% and 70% water contents were stable for 180 days at room temperature, with a mean droplet size below 12 nm and polydispersity index less than 0.24. They also exhibited higher stability and enhanced biological activities compared to free TaEO. Loquat fruit treated with TaEO-ME displayed a reduced decay index and lower membrane lipid peroxidation after 15 days of storage at 15 °C, as indicated by the lower malondialdehyde content and higher peroxidase activity. Moreover, the TaEO-ME treatment preserved the nutrient quality by maintaining the total phenolic compounds and ascorbic acid content. Our findings suggested that TaEO-ME can be used as a substitute for chemical preservatives to keep fruits fresh.Keywords:microemulsion;Torreya grandiscv. Merrillii aril;essential oil;loquat;fruit quality
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Food Science and Technology
·
2020
·Vol. 40
·期 4
·786-793
·
IF 1.443
YANG Yi-Ni,LIANG Miao,YANG Yan,ZHENG Fu-Ping,WANG Xing-Ping,YU Ai-Nong
This study optimized using a headspace solid-phase microextraction (HS-SPME) method for extracting volatile aroma compounds, terpenoids in particular, from Litsea mollis Hemsl. immature fruit (LMIF). Gas chromatography-mass spectrometry was used to separate and identify volatile terpenoids in LMIF. The types of SPME fiber coating, salt addition, and desorption time were optimized by single factor-experiments to determine the range of extraction temperature. Built on insights from the single factor-experiments, the response surface methodology was further used to optimize the extraction temperature, extraction time, and equilibrium time of HS-SPME. The results showed that the optimal HS-SPME conditions for extracting volatile terpenoids from LMIF were: 50/30 μm of DVB/CAR/PDMS SPME fiber, 2.0 g of added sodium chloride, desorption at a temperature of 250 °C for 3 min, extraction and equilibrium temperature of 46 °C, extraction time of 36 min, and equilibrium time of 20 min. Under the above conditions, the predicted value of extraction was 66.92, while the experimental value was 65.78. The prediction value matched well with the experimental value with good repeatability. The designed model was proven to be valid, which can be applied for future extraction of aroma compounds from IMIF.
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Food & Function
·
2020
·Vol. 11
·期 4
·2953-2968
·
IF 4.171
Xiaobing Yang,Wenjing Mo,Chuanjin Zheng,Wenzhi Li,Jian Tang,Xiaoyong Wu
Non-alcoholic fatty liver disease is associated with gut microbiota, oxidative stress, and inflammation. We aimed to investigate the possible mechanism by which noni fruit polysaccharide (NFP) improved hepatic oxidative stress and inflammation in rats under a high-fat diet (HFD) by modulating short-chain fatty acids (SCFAs), the intestinal barrier, and gut microbiota. Hepatic oxidative stress, inflammation, and gut dysbiosis in rats were induced through HFD feeding for 4 weeks, followed by intervention with NFP treatment (100 mg per kg bw) for 5 weeks. The results showed that NFP reduced body weight gain and improved lipid metabolism, hepatic oxidative stress, and inflammation in rats under a HFD. Aside from these beneficial effects, NFP positively affected the SCFA production and reversed the HFD-induced gut dysbiosis as indicated by improved microbiota diversity and composition. The levels of Lactobacillus, Ruminococcaceae_UCG_014, Parasutterella, [Eubacterium]_coprostanoligenes_group, and Ruminococcus_1 improved, whereas the levels of Prevotella_9, Collinsella, Bacteroides, and Turicibacter decreased. Furthermore, NFP maintained the colonic barrier integrity (increased the mRNA relative expression of CCL5, ZO-1, and occludin in the colon, and decreased the serum CCL5 level), and decreased the serum lipopolysaccharide level. Thus, NFP may modulate the gut microflora and SCFA production and reduce the permeability of the colonic barrier and metabolic endotoxemia, thereby alleviating hepatic oxidative stress and inflammation in rats under a HFD.
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JOURNAL OF HAZARDOUS MATERIALS
·
2021
·
IF 10.588
Yi Hu,Dezhi Chen,Rui Zhang,Yuan Ding,Zhong Ren,Maosheng Fu,Xiukun Cao,Guisheng Zeng
Waste-derived biochar has been emerged as promising catalysts to activate peroxymonosulfate (PMS) for the degradation of organic contaminants . Herein, passion fruit shell derived biochar (PFSC) was prepared by a one-pot pyrolysis method and used as a metal-free catalyst to activate PMS for the degradation of tetracycline hydrochloride (TC). The batch experiments indicated that the pyrolysis temperature could influence the efficiency of PFSC for the activation of PMS. In the PFSC-900 (prepared at 900 °C)/PMS system, the degradation rate of TC can reach 90.91%. The quenching test and electron paramagnetic resonance spectra revealed that the high catalytic performance of PFSC-900/PMS system was mainly attributed to the non-free radical reaction pathway containing a carbon bridge, and the TC degradation was controlled primarily by singlet oxygen-mediated oxidation . Moreover, the carboxyl group of ketones and the graphite-N atoms on PFSC-900 are the possible active sites of the non-free radical pathway including direct electron transfer or the formation of O 2 •- / 1 O 2 . This study not only shows a new type of biochar as an efficient catalyst for PMS activation but also provides a way of value-added reuse of passion fruit shell.
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PROCESS BIOCHEMISTRY
·
2020
·
IF 2.952
Chong Ou-Yang,Weiming Chai,Xiaohui Xu,Shuang Song,Qiming Wei,Qian Huang,Zhengrong Zou
Inhibiting the activity of α-glucosidase and glycosylation of protein is an important way to treat diabetes mellitus and its complications. In this study, we investigated the anti-α-glucosidase activity, anti-glycation potential and structure of proanthocyanidins from fruit pulp of Clausena lansium (Lour.) Skeels. C. lansium fruit pulp proanthocyanidins showed a remarkable inhibition against α-glucosidase activity with IC 50 vaule of 0.26 ± 0.01 μg/mL in a competitive manner, and quenched the fluorescence of α-glucosidase by forming proanthocyanidin-α-glucosidase complex. Furthermore, compared to positive agent aminoguanidine (AG), the proanthocyanidins were the more significant inhibitors of non-enzymatic glycation by strongly inhibiting the formation of α-dicarbonyl compounds and advanced glycation end products. In addition, the structure of the proanthocyanidins was characterized in detail. These compounds were mainly composed of prodelphinidins, and their gallates. The main extender units, gallocatechin epigallocatechin and their gallates, were critical factor of the strong anti-α-glucosidase and anti-glycation activity. Therefore, this study authenticated a efficient α-glucosidase inhibitors and antiglycation agents, which would contribute to the development of anti-diabetic drug.
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Food Analytical Methods
·
2014
·Vol. 8
·期 2
·428-437
·
IF 1.802
Zeng Fang,Ou Jun,Huang Yufen,Li Qian,Xu Guizhi,Liu Zhongzhen,Yang Shaohai
The present paper reports the application of an optimized pre-column derivatization procedure with aminoquinolyl- N -hydroxysuccinimidyl carbamate (AQC) to the determination of 21 free amino acids in fruit juices. The method permitted the optimal separation of 21 amino acids and ammonium in 30 min. Excellent response linearity was obtained from 2 to 1,000 μM for all amino acids, except tryptophan, for which the linearity was 5–1,000 μM. The fluorescence detection limits ranged from 0.08 pmol (phenylalanine) to 0.69 pmol (cysteine), except for tryptophan (7.89 pmol), for a signal-to-noise ratio of 3. The relative standard deviations of peak areas ranged from 0.1 to 0.4 % for intraday analysis and from 2.1 to 5.0 % for interday analysis. The method was applied to analyze the free amino acids in six fruit juices, and the recoveries of the proposed method were 90.4 to 103.3 %. The total free amino acid content ranged from 56.97 mg L −1 (apple) to 469.45 mg L −1 (longan) in six fruit juices. Aspartic acid (Asp), glutamic acid (Glu), asparagine (Asn), serine (Ser), and glutamine (Gln) were present in higher levels in most samples, whereas cysteine (Cys) was below the detection limit in the selected samples. Notable advantages of this method include its speed, ease, low cost, good repeatability, high sensitivity, excellent precision and accuracy, and anti-interference in high-sugar matrices.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Tianhe Gao,Lijuan Yan,Qiuyan Yu,Yan Lyu,Weifu Dong,Mingqing Chen,Tatsuo Kaneko,Dongjian Shi
Utilizing antibacterial packaging material is an effective approach to delay fruit rotting and spoilage thereby minimizing financial losses and reducing health harm. However, the barrier and mechanical properties of biodegradable antibacterial packaging materials are barely compatible with transparency. Herein, antimicrobial nanoparticles encapsulating citral (ANPs) were first prepared by emulsification under the stabilization of oxidized dextran (ODE) and ethylene diamine. Then, composite films with high transparency, good water vapor barrier, and mechanical and antibacterial properties for fruits packaging were prepared from chitosan (CS), carboxymethyl-glucan (CMG), poly(vinyl alcohol) (PVA), and ANPs by solvent casting strategy. The synergistic effects of electrostatic interaction and hydrogen bonding could regulate crystalline architecture, generating high transparency of the composite films (90 %). The mechanical properties of the composite film are improved with elongation at break up to 167 % and stress up to 32 MPa. The water vapor barrier property of the film is appropriate to the packed fruit for less weight loss and firmness remaining. Simultaneously, the addition of ANPs endowed the film with excellent antimicrobial and UV-barrier capabilities to reduce fruit mildew, thereby extending the shelf life of fruits. More importantly, the composite polymer solution could be sprayed or dipped directly on fruits as a coating for food storage to improve food shelf life, substantially expanding its ease of use and scope of use.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Zi-Ling Li,Xiao-Run Qi,Yi-Heng Xu,Jia-Qi Zhao,Gui-Fu Zuo,Man-Man Wang
Effective and convenient QuEChERS of lipophilic pesticides with wide p K a range from strongly pigment-rich food samples remains a great challenge. Here, ZIF-67 derived magnetic nanoporous carbon (Co@MPC) was firstly proposed for modified QuEChERS of carbamate pesticides ( pK a 4.3–12.3) in various vegetable and fruit samples prior to LC-MS/MS. This sorbent offered superior adsorption capacities of 29.89 mg/g and 22.28 mg/g towards chlorophyll and lutein via π-π and hydrophobic interactions. The present method exhibited acceptable matrix effect of −4.8 %-14.4 %, low detection limits of 0.003–0.02 μg/kg and satisfactory recoveries of 80.2 %–108 % in celery, cabbage, Chinese cabbage, apple, banana and orange samples. 10 mg of Co@MPC enabled highly efficient matrix purification and isolation in 20 s, offering low-cost and facile clean-up process. The study highlighted great potential of Co@MPC as QuEChERS sorbent, and demonstrated its wide applicability and broad application prospects to monitor the contaminants in food items.
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
He Mei,Jingcheng Huang,Guixin Wang,Kun Chen,Huili Wang,Xuedong Wang,Wenwei Wang
Herein, we engineered a novel pH-responsive ratiometric assay by virtue of the composite system of Ce 3+ -enhanced glutathione-encapsulated copper nanoclusters (GSH-CuNCs-Ce 3+ ) and N-doped carbon quantum dots (N-CQDs). The GSH-CuNCs-Ce 3+ and N-CQDs were fabricated using one-pot chemical reduction and hydrothermal approaches, respectively, and their morphologies as well as physical and chemical properties were testified in detail by a series of characterization techniques. At 350 nm excitation wavelength, the GSH-CuNCs-Ce 3+ /N-CQDs system (GCC-NC) featured the 440/650 nm dual-emitting property. The ratios of fluorescence intensities (F 650 /F 440 ) demonstrated a strong pH-dependence from 3.0 to 5.0 with a coefficient of determination of 0.9982. Consequently, the GCC-NC system was feasible for constructing a pH-responsive dual-emitting fluoroprobe. Based on the catalytic effect of acetylcholinesterase on acetylcholine to yield acetic acid and further trigger varying solution pH, the as-constructed GCC-NC fluoroprobe was satisfactorily applied for detecting malathion . Under optimized conditions, the newly developed GCC-NC fluoroprobe supplied a wider linear range (0.25–200 μM), lower detection limit (0.075 μM), satisfactory recoveries (96.3–106.2%) and higher precision for malathion in several kinds of fruit juice samples. The fortified experiments by malathion’s structural analogues and metal ions provided compelling evidence that this fluoroprobe had strong anti-interference capacity and high specificity for malathion. These findings render us to believe that the as-constructed pH-responsive dual-emitting fluoroprobe holds great promise in trace malathion assay in food matrices.
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POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2024
·
IF 6.4
Juan Ma,Shuang Liu,Jing Zeng,Yiwen Zhang,Wei Chang,Zhengkun Meng,Yujia Zhou,Wene Zhang,Xiaochun Ding,Xuejun Pan,Xuewu Duan
Rosa roxburghii has a short and concentrated harvest period, during which rapid decay and quality deterioration at room temperature pose significant challenges to the supply chain. To address this, we applied methyl jasmonate (MeJA) treatment and stored the fruit at low temperatures. MeJA treatment effectively reduced decay, maintained fruit firmness and brightness, suppressed respiration, and decreased malondialdehyde content. Further analysis revealed that MeJA reduced hydrogen peroxide levels by boosting the activities and gene expressions of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD). Additionally, MeJA upregulated the expression of disease resistance-related genes ( RrRGA3, RrPPO, RrCHIT, RrPRB1 , and RrRPM1 ). It also stimulated genes involved in the AsA synthesis and AsA-GSH cycle ( RrMIXO, RrAKRC9, RrDHAR , and RrGPX ), thereby increasing AsA content. Moreover, MeJA promoted the activities (PAL, C4H, and 4CL) and gene expressions ( RrPAL, Rr4CL, RrCSE, RrCCR, RrPGT, RrHCT , RrDFR and RrERF114 ) of phenylpropane metabolism, resulting in increased levels of L-phenylalanine, caffeic acid, phlorizin, and other phenolic acids and lignin content. Furthermore, MeJA induced the expression of genes related to JA biosynthesis ( RrAOC, RrOPR , and RrACX ), and abscisic acid synthesis ( RrNCED ). In conclusion, these findings suggest that MeJA treatment enhances disease resistance and preserves the postharvest quality of R. roxburghii , making it a promising preservation method for large-scale commercial application in fruit storage.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Liangrui Lv,Qin Tao,Yijing Kuang,Tingting Zhang,Xiaoying Wang
Patulin (PAT) is a widespread fruit toxin. Trace-level PAT exposure can cause serious harm to human health. Herein, a multimodal PAT aptasensor was designed based on Ru(bpy) 3 2+ -based metal organic framework composited hydrogel (RuMOF@hydrogel) and versatile banana peel-derived carbonized polymer dots (BPPDs). RuMOF@hydrogel modified magnetic-electrode exhibited excellent anodic and cathodic electrochemiluminescence (ECL) emission and stability. Meanwhile, the BPPDs could enhance anodic ECL of RuMOF@hydrogel, and also show excellent fluorescence (FL) and photothermal (PT) properties. With the aid of PAT-triggered hybridization chain reaction and magnetic separation, ECL, FL, and PT responses could be recorded concurrently. The detection limit can reach as low as 0.25 fg mL −1 . The ratiometric ECL quantitation ensured the sensitivity and accuracy of this assay. And visual FL and portable PT modes contributed to the utility. Furthermore, this aptasensor demonstrated better performances than HPLC in fruit products and the protocol can be extended to determine various contaminants in foods.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Yulin Xu,Qin Luo,Lichun Yang,Shenghao Zhang,Yuanbo Huang,Yan Sun,Wei Wei,Min Xie,Yongjun Hu
Sensitive, flexible and rapid SERS substrate for the on-site detection of pesticide residues on the fruit peel is of great significance. In this study, the three-dimensional nanofiber network of bacterial nanocellulose (BNC) serves as a matrix template material in the composite system. The highly antioxidative silver@gold nanoparticles (Ag@Au NPs), featuring a ∼ 45 nm diameter Ag core and an ultrathin ∼2 nm Au shell, were first synthesized via a ligand-assisted complexation approach. Subsequently, these nanoparticles were uniformly incorporated into the bacterial nanocellulose (BNC) matrix through a facile vacuum filtration method to fabricate the flexible Ag@Au NPs-BNC SERS substrate. The prepared flexible SERS substrate achieved an exceptional detection sensitivity of 5 × 10 −9 M for the detection of crystal violet (CV), excellent signal uniformity (RSD = 5.4 %), and exhibited a remarkable SERS enhancement factor of 1.19 × 10 6 . The substrate demonstrated excellent performance for detecting thiram in apple and pear peels, exhibiting a linear dynamic range of 0.1–10 ppm with ultralow detection limits (LODs: 0.0498 ppm and 0.0621 ppm, respectively), and these values are two orders of magnitude below the U.S. Environmental Protection Agency's maximum residue limit (MRL: 5 ppm) for thiram in agricultural products. Remarkably, the substrate maintained stable SERS signal intensity after 57 days of ambient storage, confirming its outstanding long-term stability. The flexible Ag@Au NPs-BNC SERS substrate offers high sensitivity, excellent stability, and reliable detection of trace hazardous chemicals in real applications.
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