BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
·
2025
·
IF 2.2
Qiuni Long,Hua Huang,Xinshuai Zhang
l -ergothioneine is a naturally occurring histidine thio-derivative produced by certain bacterial and fungal species. Plants acquire ergothioneine from soil, while animals ingest it through their diets. Ergothioneine is widely acknowledged for its powerful antioxidant properties, capable of scavenging reactive oxygen and nitrogen species, thereby exhibiting promising applications across various industrial fields such as cosmetics, functional foods, and medicine. The catabolism of ergothioneine in the biosphere is quite diversified, in this study, we successfully analyzed and experimentally characterized an oxidative catabolic pathway of ergothioneine encoded by Actinoplanes missouriensis ATCC 14538. In this pathway, ergothioneine is firstly oxidized into ergothioneine sulfinate under the catalysis of a dioxygenase (PF05995). Then, ergothioneine sulfinate is converted into hercynine by releasing sulfite with an aminohydrolase (SSF51556). Finally, hercynine is transformed into urocanate, a known metabolite in histidine catabolism, by an aromatic amino acid lyase (PF00221). This catabolic pathway is primarily present in Actinobacteria, a group of soil microorganisms that may capable of recycling sulfur of ergothioneine through this pathway.
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产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2025
·
IF 6.1
Jiezhen Huang,Zheng Huang,Na Chen,Qiufen Li,Yiyuan Fu,Xiaolong Jin,Xiaohui Xie,Honggang Li,Xiaoyan Wan
Increasing evidence indicates that the maternal preconception environment and lifestyle affect offspring health; however, the effects of paternal preconception environment and lifestyle have rarely been examined. Therefore, we aimed to examine whether paternal infertility-related factors affect offspring produced via assisted reproductive technology in mice. Scrotal heat impairment, which can result from factors such as varicoceles, sauna use, physical inactivity, or occupations such as welding, is a common risk factor associated with reduced male fertility. To model these effects, we exposed the scrota of C57BL/6 J male mice to 39 °C water for 30 min once weekly for 5 consecutive weeks, establishing frequent and mild scrotal heat stress (fmSHS) that mimics human SHS. Sperm from these mice, along with untreated female oocytes, were used for in vitro fertilization with embryo transfer to produce first-generation (F1) offspring. Second-generation (F2) offspring were obtained by mating F1 males with untreated females. Reduced spatial memory was observed in F1 males derived from fmSHS-exposed fathers, whereas F2 fmSHS males showed no significant impairment in spatial-memory testing. In F1 fmSHS offspring, hippocampal histology and N-methyl-D-aspartate receptor expression did not differ from controls. However, genes involved in the long-term potentiation pathway were dysregulated in the hippocampi of F1 fmSHS males, and abnormal methylation of the Pi3kcg promoter was observed in both the sperm of fmSHS fathers and the hippocampi of F1 males. These findings highlight the adverse effects of paternal preconception scrotal heat stress on offspring health.
研究领域:
生殖生物学
·
表观遗传学
·
神经科学
·
发育生物学
·
环境健康
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2019
·
IF 3.218
Zheng-Guang Hu,Zhi-Yuan Tan,Zhong Lin,Jun Chen,Fang Sun,Xuyao Tang,Rui-Ting Zheng,Yong-Chong Chen,Guo-An Cheng
Si is one of the most attractive anode materials because of its extremely high theoretical capacity. In this study, we find that the (111) plane of crystalline Si in the anode is still present after 100 cycles and infer that it is the intercalation site of Li + ions. We believe that Li + ions are inserted into the tetrahedral sites of crystalline Si, and the (111) plane of Si collapses; meanwhile, expansion parallel to 〈220〉 crystal orientation occurs. After full lithiation , the crystalline Si is converted to amorphous silicon . In a Si/ composite anode, although the addition of amorphous carbon may improve the cycling performance of Si anode, the activation process is prolonged as the amount of amorphous carbon increased. We determine that amorphous carbon may reduce the diffusion of Li + , which increases the overpotential and results in the lower formation of the saturated Li/Si alloy.
研究领域:
材料科学
·
电化学
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Kai Wang,Miao-Yan Wang,Chen-Xi Cao,Yu-Shun Yang,Ai-Guo Zhang,Ping-Gui Li,Chen-Yang Han,Hao-Xiang Jiang
Recent investigations have revealed the tight connections between carboxylesterase (CE) and the lipid metabolism-associated disorders. In this work, based on the previous reports, a photoacoustic (PA) probe Hcy-CE was developed for the in vivo imaging of CE in two lipid metabolism disorders. The reaction with CE led to the departure of the carbamate quencher together with the linkage, thus to release of the reporter Hcy to exhibit the turn-on signals of PA reporting accompanied with the absorbance and fluorescence modes. The responses in all three modes showed high consistency. In the PA mode, the linear correlation range was CE level 0–10 U/L, while the limit of detection value was 0.52 U/mL. The optical performance of the probe Hcy-CE in the solution system was practical with high steadiness and selectivity. Moreover, Hcy-CE exhibited potential capability of monitoring the CE level in the induced foam cells. In particular, Hcy-CE realized the in vivo imaging of the CE level from multiple views and continuous cross-sections in the model mice of different groups. The combination of the PA imaging with the blood biochemical tests and immunohistochemically staining methods might help distinguish the three statuses of different mice from each other. This work provided meaningful information for the accurate diagnosis and regulation of the lipid metabolism-related disorders.
研究领域:
生物医学工程
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分子生物学
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成像科学
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Chun-Pu Mao,Xu-Yang Chen,Jie Han,Ting Jiang,Xiao-Xin Yan,Dong-Lin Hao,Jian-Hua Jin,Biao Yu,Jie-Li Zhou,Kai Wang,Li-Ting Zhang
Lipid metabolic diseases have become an important challenge to global public health. Along with lifestyle changes, the incidence of obesity, diabetes and other metabolic syndromes is on the rise, and the number of patients with fatty liver disease is also increasing. Therefore, it is particularly important to develop effective lipid imaging strategies to monitor and manage fatty liver disease. Herein, based on the essential role of alkaline phosphatase (ALP) in both AS and OB, in vivo imaging of ALP was achieved in two lipid metabolic diseases models with a photoacoustic (PA) probe phosphorylated hemicyanine ( P-Hcy ). After being triggered by ALP, P-Hcy responded in different modalities including absorbance, fluorescence and, most significantly, PA-reporting. Notably, the PA signal showed the reliable linear correlation to the ALP level within the range of 0–800 U/L. The probe P-Hcy exhibited the advantages including high sensitivity, high selectivity, and steadiness in required biological conditions. The intracellular imaging results ensured that P-Hcy could visualize the ALP level in the foam cells induced from mouse mononuclear macrophages. In the healthy and lipid metabolic diseases models, P-Hcy was able to distinguish well between a lipid metabolic disease model and a healthy mouse model by photoacoustic imaging. By combining the ALP detection with P-Hcy in PA/fluorescence modality and traditional techniques such as blood biochemical testing and immunohistochemically staining, more potential strategy to accurately diagnose lipid metabolic diseases in the pre-clinical trials might be developed in future.
研究领域:
医学影像
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代谢疾病
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生物化学
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药学
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DOI
PubMed
高影响因子
Natural Product Communications
·
2024
·
IF 1.5
Liu Tang,Tianshuai Wang,Ruzhen Peng,Jingyi Guo,Liping Li,Benhong Zhou,Guangwen Luo,Shasha Fang,Xiao Jiang
Background: Atopic dermatitis, often referred to as AD, is a common chronic and relapsing skin disease with few treatment options. Epigallocatechin 3-gallate (EGCG), the most abundant catechin derived from green tea, has gained widespread attention due to its excellent anti-inflammatory and anti-oxidative properties. This research aims to investigate the effect and mechanism of EGCG in alleviating AD by integrative approach of an in vivo AD mouse model, network pharmacology and molecular docking. Materials and Methods: EGCG was administered by intragastric administration at the dose of 100 mg/kg, once daily for 14 consecutive days to AD mice, a model established by repeated application of 2, 4-dinitrofluorobenzene (DNFB) to the mouse dorsal skin. Results: Macroscopical and histopathological examination showed that oral administration with EGCG could markedly relieve AD-like skin phenotypes, as evidenced by a substantial reduction in severity score, serum immunoglobulin E (IgE) level, epidermal thickness, and the infiltration of inflammatory cells and mast cells into the dermal layers. Mechanistically, the network pharmacology analysis indicated that the protective effects of EGCG against AD were mainly related to the regulation of AGE-RAGE signaling pathway in diabetic complications, Th17 cell differentiation, IL-17 signaling pathway, NF-κB signaling pathway, Toll-like receptor signaling pathway, TNF signaling pathway, etc. In silico molecular docking demonstrated the high binding affinity of EGCG to the core targets such as ABCB1, MMP2, MMP9, MAPK14 and STAT1. Furthermore, western blotting (WB) results showed that EGCG obviously downregulated the expression of RAGE and STAT1, thereby regulating the AGE-RAGE signaling pathway to improve AD. Conclusions: These findings indicated that EGCG can be a promising therapeutic candidate for AD.
研究领域:
药理学
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皮肤病学
·
分子生物学
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DOI
高影响因子
JOURNAL OF HETEROCYCLIC CHEMISTRY
·
2024
·
IF 2
Ling Li,Yinuo Yao,Xiaowan Wang,Yaoqi Yu,Tailong Ji,Han Li,Yi Li,Yuanyuan Liu
A highly selective fluorescent sensor Por-RBO for determination of Hg 2+ was designed and synthesized with Rhodamine B and Tetraphenylporphyrin. The sensor can determine the Hg 2+ of a solution within the pH 5.0–8.0, free from interference of other metal ions. When detected in buffer solution, the fluorescence was the strongest when five times the concentration of Hg 2+ ion was added. Calculated by fluorescence titration method, the detection limit of sensor Por-RBO for Hg 2+ ion was as low as 0.12 μmol/L. The geometries of Por-RBO and Por-RBO -Hg 2+ were optimized at the B3LYP/6-31G** level by density functional theory. The charge distribution, orbital interactions, and bonding characteristics were analyzed and compared in detail to discuss the recognition mechanism and structure–fluorescence property relationships. The results of cell fluorescence imaging and CCK-8 experiments indicate that the sensor Por-RBO exhibits lower cytotoxicity. Por-RBO can be used for fluorescence distribution imaging of Hg 2+ in living cells.
研究领域:
分析化学
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生物医学工程
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环境科学
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DOI
高影响因子
Journal of Saudi Chemical Society
·
2021
·
IF 3.932
Smaher M. Elbayomi,Xuan Nie,Ye-Zi You,Tamer M. Tamer
Cationic polymers with high charge density could effectively condense the DNA and achieve gene transfection; however, it often brings non-negligible cytotoxicity. Notably, the high charge density gene vector fails in the serum environment, limiting further application in vivo. In this paper, an efficient and reliable non-viral gene vector of poly (amidoamine) (PAA) was designed by introducing diacryolyl-2,6-diaminopyridine (DADAP) onto the PAA backbone through Michael-addition polymerization, which provides high transfection efficiency in a serum-containing environment. Diacryolyl-2,6-diaminopyridine and cationic parts provided multiple interactions between gene vectors and DNA, including hydrogen bond and electrostatic interactions. The introduction of hydrogen bonding can effectively reduce the charge density of polyplexes without reducing the DNA condensing ability, incorporating the diaminopyridine group and cationic part in PAA chains successfully consolidated cellular uptake, endosome destabilization, and transfection efficiency for the PAA/DNA complexes with low cytotoxicity. The constructed vector with multiple interactions presented 6 times higher transfection efficiency in serum-free and 9 times in serum-containing environment than that of branched polyethyleneimine (PEI 25K) in 293T cells in vitro. Therefore, introducing the hydrogen band to form low charge density polyplexes with high transfection efficiency and low cytotoxicity has a great potential in gene delivery.
研究领域:
生物化学
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分子生物学
·
基因治疗
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DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 24
·期 1
·275
·
IF 6.208
Fei Li,Honglu Zhao,Yuxin Liu,Jiaqi Zhang,Hongbo Yu
Lytic polysaccharide monooxygenases (LPMOs) have the potential to improve recalcitrant polysaccharide hydrolysis by the oxidizing cleavage of glycosidic bond.Streptomycesspecies are major chitin decomposers in soil ecological environments and encode multiplelpmogenes. In this study, we demonstrated that transcription of thelpmogene,Sclpmo10G, in theStreptomyces coelicolorA3(2) (ScA3(2)) strain is strongly induced by chitin. TheScLPMO10G protein was further expressed inEscherichia coliand characterized in vitro. TheScLPMO10G protein showed oxidation activity towards chitin. Chitinase synergy experiments demonstrated that the addition ofScLPMO10G resulted in a substantial in vitro increase in the reducing sugar levels. Moreover, in vivo the LPMO-overexpressing strainScΔLPMO10G(+) showed stronger chitin-degrading ability than the wild-type, leading to a 2.97-fold increase in reducing sugar level following chitin degradation. The total chitinase activity ofScΔLPMO10G(+) was 1.5-fold higher than that ofScA3(2). In summary,ScLPMO10G may play a role in chitin biodegradation inS. coelicolor, which could have potential applications in biorefineries.Keywords:lytic polysaccharide monooxygenases;transcription;expression;Streptomyces coelicolorA3(2);chitin biodegradation
研究领域:
微生物学
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生物化学
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DOI
PubMed
高影响因子
Food & Function
·
2018
·Vol. 9
·期 4
·2251-2260
·
IF 3.289
Guo Liu,Shengwei Sun,Baoyan Guo,Benchun Miao,Zhen Luo,Zumeng Xia,Danyang Ying,Fei Liu,Bin Guo,Jian Tang,Yong Cao,Jianyin Miao
Casein phosphopeptides (CPPs) have been demonstrated to be calcium chelators. Unfortunately, few studies have been reported on the effects of CPPs on the mechanism of the uptake and absorption of Ca2+ and bone metabolism. In this study, a monomeric peptide fraction isolated by RP-HPLC (F6-1) that possessed high calcium transport capacity in Caco-2 cell monolayers was separated and characterized. The effects of F6-1 on the absorption mechanisms of Ca2+ in a Caco-2 monolayer model and bone metabolism in rats were investigated. F6-1 was isolated by preparative and analytical RP-HPLC. Results for calcium transport suggested that the rates of Ca2+ transportation by F6-1 were approximately 2.57, 2.87 and 2.38 times higher than those in the control group at 30, 60 and 120 min, respectively. Results of ultraviolet (UV) spectroscopy indicated that the intensity of UV absorption changed because of the binding of Ca2+ to F6-1. Analysis of transepithelial electrical resistance (TEER) and the expression of TRPV6 in Caco-2 cells showed that F6-1 was likely to influence the transcellular pathway of intestinal absorption of Ca2+ rather than the paracellular pathway. Furthermore, the F6-1 group (1% Ca, 0.03% F6-1) exhibited increases in serum Ca2+ levels, femur length and femur Ca and decreases in serum alkaline phosphatase (ALP) levels and urinary pyridinoline content in a Sprague-Dawley rat model, which implied that F6-1 was beneficial for bone calcification. Overall, our results suggested that F6-1 enhanced the transport of Ca2+ in Caco-2 cells by affecting the transcellular pathway by upregulating the expression of TRPV6. F6-1 also improved bone formation and prevented bone resorption to benefit bone health in rats, which provided a basis for using F6-1 in calcium supplements or functional foods.
研究领域:
营养学
·
生物化学
·
食品科学
·
生理学
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DOI
PubMed
高影响因子
Journal of Biomaterials and Tissue Engineering
·
2017
·Vol. 7
·期 9
·848-857
·
IF 1.383
Li Qianwen,Cai Tiange,Huang Yinghong,Zhang Ronghua,Cole Susan P. C.,Cai Yu
This study was designed to prepare Cepharanthine-nanostructured lipid carriers (CEP-NLC) and investigate their structure, physical and chemical properties, in vitro release and in vivo pharmacokinetics. CEP-NLCs were prepared by the emulsification evaporation-low temperature
solidification method. Particle size and encapsulation efficiency as CEP-NLCs evaluation indexes to optimize formulation through Box-Behnken. Optimal formulation: total lipid content 313.0 mg, Cepharanthine 4.4 mg and tween-80 3%. The CEP-NLC prepared according this formulation possessed a
round appearance, were of uniform size, exhibited no adhesion, had an encapsulation efficiency of (98.19±0.48)%, and cold storage stability. Using 5% mannitol as a freeze-drying protective agent, the quality freeze-dried powder of CEP-NLC was excellent. CEP-NLC had a sustained-release
effect, a good biocompatibility when the concentration of CEP was lower than 80 μg/ml. In vivo experiments showed that CEP-NLC increases the blood concentration and bioavailability of CEP and in mice altered the drug tissue distribution of CEP, showing preferential targeting
to the lung. In summary, the optimization of formulation and preparation, successfully prepared excellent physicochemical properties and high bioavailability of CEP-NLC.
研究领域:
药剂学
·
纳米技术
·
药物递送系统
·
药理学
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DOI
高影响因子
JOURNAL OF NANOPARTICLE RESEARCH
·
2018
·Vol. 20
·期 3
·1-11
·
IF 2.127
Han Congcong,Yang Jian,Gu Jinlou
Silver nanoparticles (AgNPs) are a potential class of nanomaterial for antibiosis and chemotherapeutic effects against human carcinoma cells. However, the DNA-damaging ability of free AgNPs pose the critical issues in their biomedical applications. Herein, we demonstrated a facile method to capture Ag + ions and reduce them into active AgNPs within Zr-based metal-organic frameworks (MOFs) of UiO-66 with a mild reductant of DMF (AgNPs@UiO-66(DMF)). The average diameters of UiO-66 carriers and AgNPs were facilely controlled to be 140 and 10 nm, respectively. The obtained UiO-66 nanocarriers exhibited excellent biocompatibility and could be effectively endocytosed by cancer cells. Additionally, the AgNPs@UiO-66(DMF) could rapidly release Ag + ions and efficiently inhibit the growth of cancer cells. The half maximal inhibitory concentration (IC 50 ) values of the encapsulated AgNPs were calculated to be 2.7 and 2.45 μg mL −1 for SMMC-7721 and HeLa cells, respectively, which were much lower than those of free AgNPs in the reported works. Therefore, the developed AgNPs@UiO-66(DMF) not only maintained the therapeutic effect against cancer cells but also reduced the dosage of free AgNPs in chemotherapy treatment.
A mild reduction process was developed for the fabrication of AgNPs@UiO-66, which exhibited efficient induction of apoptosis in cancer cells.
研究领域:
纳米技术
·
癌症研究
·
材料科学
·
生物医学工程
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DOI
高影响因子
危险品化学品经营许可证（带存储）