MOLECULES
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2021
·Vol. 26
·期 11
·3064
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IF 4.412
Qing Shi,Ling-Yi Shen,Hong Xu,Zhi-Yong Wang,Xian-Jiong Yang,Ya-Li Huang,Carl Redshaw,Qi-Long Zhang
A novel 1-hydroxy-2,4-diformylnaphthalene-based fluorescent probeLwas synthesized by a Knoevenagel reaction and exhibited excellent sensitivity and selectivity towards sulfite ions (SO32−) and bisulfite ions (HSO3−). The detection limits of the probeLwere 0.24 μM using UV-Vis spectroscopy and 9.93 nM using fluorescence spectroscopy, respectively. Furthermore, the fluorescent probeLcould be utilized for detection in real water samples with satisfactory recoveries in the range 99.20%~104.30% in lake water and 100.00%~104.80% in tap water by UV-Vis absorption spectrometry, and in the range 100.50%~108.60% in lake water and 102.70%~103.80% in tap water by fluorescence spectrophotometry.Keywords:fluorescent probe;sulfite/bisulfite;crystal structure;real sample detection
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ANALYTICA CHIMICA ACTA
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2020
·
IF 5.977
Zhenya Yu,Xue Chen,Ying Cheng,Hongmei Yang,Fang Wang,Zilin Chen
A label-free electrochemical method was developed for sensitive determination of ten-eleven translocation protein 1 (TET1) which can mediate the demethylation of DNA . This strategy is mainly based on MspI-mediated restriction endonuclease reaction. Current response difference of the biosensor before and after cleavage by MspI was dependent on the activity and concentration of TET1. With the aid of Au nanoparticles , this method shows a good linear range from 0.0042 μg μL −1 to 0.0210 μg μL −1 with a correlation coefficient of 0.9350 and a low limit of detection 0.00098 μg μL −1 . Finally, this method was used to investigate the effects of n-oxalylglycine (NOG) and taxol on activity of TET1. The results indicated that NOG could inhibit TET1 activity but taxol could not. So this electrochemical biosensor could be applied to TET activity evaluation and inhibitor screening in field of biomedicine and clinical diagnosis.
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BIORESOURCE TECHNOLOGY
·
2020
·
IF 7.539
Jun Tu,Jianbo Guo,Caicai Lu,Haibo Li,Yuanyuan Song,Yi Han,Yanan Hou
Cyclodextrins (CDs) have been widely used due to the excellent solubilization of hydrophobic organics. However, their effect on the biotransformation process of hydrophilic pollutants is unclear. This study first evaluated the effect and mechanism of CDs on nitrate reduction by S.oneidensis.MR-1 . The three CDs (α-CD, β-CD and γ-CD) all accelerated nitrate reduction, among which β-CD had the best effect. The nitrate reduction rate was increased by 21.8% with 0.5 mM β-CD. As for the mechanism, β-CD increased the biomass, membrane permeability and EPS of S.oneidensis.MR-1 . The nitrate reductase activity was also increased by 1.34-fold with 0.5 mM β-CD. The current exchange density and the electron transfer system activity were increased by 11.4% and 99.5% in the β-CD-supply system, respectively. It confirmed that β-CD enhanced the biological and electrochemical characteristics and then enhanced bio-activity. This study provides a new understanding of CDs in microbial remediation and broadens the practical application.
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SENSORS AND ACTUATORS B-CHEMICAL
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2020
·
IF 7.1
Yong Liu,Ensheng Xu,Chao Xu,Wei Wei
As a tumor-related enzyme, PARP-1 activity is closely related to the occurrence of cancer. Sensitive detection of PARP-1 activity is of significance in the early diagnosis and treatment of cancer. Therefore, the gold nanorods (AuNRs) based biological nanoprobe was constructed for the visual and label-free detection of PARP-1 activity. dsDNA which activate PARP-1 was modified on AuNPs ( [email protected] ). MoO 4 2− is added to catalyze H 2 O 2 and KI to accelerate the etching of AuNRs. In the presence of PARP-1, hyperbranched poly (ADP-ribose) polymers (PAR) formed on the surface of [email protected] ( [email protected] @PAR). A large number of PO 4 3- of the PAR reacted with MoO 4 2− to generate PMo 12 O 40 3- . Thus, PO 4 3- diminished MoO 4 2− concentration in solution, preventing the etching of AuNRs. Therefore, PARP-1 prevented the etching of AuNRs. With the increasing concentration of PARP-1, the color of the solution changed from light blue to brown obviously. The method was applied to assay actual samples with satisfactory results. The strategy has the advantages of rapid quantitative analysis of PARP-1 activity with naked eyes, simple operation and good application prospect.
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Catalysts
·
2020
·Vol. 10
·期 11
·1304
·
IF 3.52
Qiang Yuan,Jifeng Pang,Wenguang Yu,Mingyuan Zheng
A high-performance Pt catalyst supported on SBA-15 was developed for furfural decarbonylation. Compared to Pt catalysts loaded on microporous DeAl-Hbeta zeolite and hierarchical micro-mesoporous MFI nanosheet (NS) materials, the 1%Pt/SBA-15 catalyst afforded notably higher activity, furan selectivity and stability owing to the negligible acid sites and proper mesopores on the SBA-15 support. Among a set of 1%Pt/SBA-15 catalysts bearing Pt nanoparticles (NPs) with sizes of 2.4–4.3 nm, the catalyst with 3.7 nm Pt NPs afforded the highest furan selectivity. Over the optimal catalyst, 88.6% furan selectivity and ca. 90% furfural conversion were obtained at 573 K and a high weight hourly space velocity (WHSV) of 16.5 h−1. Moreover, the reaction temperatures at 440–573 K and the ratios of H2to furfural at 0.79–9.44 did not affect the reaction selectivity notably, showing that the reaction over 1%Pt/SBA-15 can be conducted over a wide range of conditions. The catalyst was stable under the harsh reaction conditions and lasted for 90 h without significant deactivation, demonstrating the superior property of SBA-15 as a catalyst support for furfural decarbonylation.Keywords:decarbonylation;furfural;furan;platinum;SBA-15
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Chinese Journal of Natural Medicines
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2020
·
IF 2.014
Li YAO,Yu-Xia YANG,Hui CAO,Huan-Huan REN,Zheng NIU,Ling SHI
Osthole is observed to have the capacity to treat pulmonary arterial hypertension (PAH) in rats, but molecular mechanism is still unknown. The present study aims to discover therapeutic targets and explore therapeutic mechanism of osthole against PAH from metabolic perspective. A rat model with PAH was successfully established with MCT, following osthole administration, then untargeted metabolomics assay was performed using UPLC-Q-TOF-MS to identify differential metabolites and associated metabolic pathways, at last mechanism investigation was done by qRT-PCR, Western blot and ELISA. Differential metabolites characterized in rats with PAH were mostly assigned to sphingolipid metabolism, synthesis of unsaturated fatty acids, glycolysis, nucleotide metabolism, steroid hormone biosynthesis. Furthermore, osthole reversed high level of S1P by modulating metabolic enzyme Sphk1 in rats with PAH. In addition, osthole inhibited the expression of Sphk1 by downregulating microRNA-21, phosphorylation of Akt, phosphorylation of mTOR in vivo and in vitro . These results demonstrated that metabolomics is a promising approach to discover potential drug target for PAH treatment. Importantly, our findings further elucidated therapeutic mechanism of osthole, a natural product, having a role of metabolic regulator to potentially treat PAH by targeting inhibition of Sphk1/S1P via microRNA-21-PI3K/Akt/mTOR signal pathway. Altogether, this discovery paves a critical foundation for enabling osthole to be a candidate compound to treat PAH.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
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2020
·Vol. 31
·期 13
·10639-10648
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IF 2.22
Si Shufang,Deng Hongmei,Wang Tiantian,Zheng Dongliang,Yang Pingxiong,Chu Junhao
Multiferroic ferrite–ferroelectric composites with general formula x CoFe 2 O 4 -(1 − x )K 0.5 Bi 0.5 TiO 3 ( x = 0.0, 0.3, 0.5, 0.7, and 1.0) ( x CFO-KBT) were developed by conventional solid-state reaction method. The structural, optical, magnetic and ferroelectric properties for the prepared samples were studied in detail. Two individual structures of spinel-type ferrite and tetragonal-type ferroelectric are characterized through X-ray diffraction while Raman scattering spectra and SEM analyses furtherly confirm the coexistence of the two phases in the composites. The bandgaps calculated for the composites narrow dramatically compared with the pure KBT, which are attributed to Co 2+ d–d on-site transitions from the CFO phase. The magnetic property transforms from diamagnetism for pure KBT to ferromagnetism for the composites and pure CFO with saturation magnetization M s increasing gradually with addition of CFO. Besides, the room temperature ferroelectricity has been detected in the composites, which turns out to be enhanced by space charge contribution of CFO phase. The improvement of optical and multiferroic properties in the composites indicates great prospect on visible-light optical devices and non-volatile data storage.
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CHINESE JOURNAL OF CATALYSIS
·
2021
·
IF 8.271
Yuyao Wang,Li Li,Risheng Bai,Shiqin Gao,Zhaochi Feng,Qiang Zhang,Jihong Yu
Tailoring the Ti coordination states in titanosilicate zeolites to simultaneously improve feedstock conversion and maximize the target product selectivity remains a challenge in the pursuit of high-performance catalysts for selective oxidation reactions. Herein, we provide a facile strategy to synthesize hierarchical anatase-free TS-1 ( MFI -type) zeolites with tetrahedrally coordinated (TiO 4 ) and octahedrally coordinated Ti species (TiO 6 ). The TiO 4 species provide high epoxide selectivity, while the TiO 6 species afford improved alkene conversion. This strategy is achieved by synergistically using an L-lysine-assisted approach and a two-step crystallization; the two-step crystallization approach prevents the formation of anatase TiO 2 , while L-lysine stabilizes the TiO 6 species and ensures efficient incorporation of TiO 6 into the anatase-free TS-1 zeolites. Compared with their conventional counterparts, which only contain TiO 4 species, the as-prepared TS-1 zeolites (Si/Ti = 36.9) result in a higher 1-hexene conversion (33%), higher TON value (153), and comparable epoxide selectivity (95%). This synthetic strategy provides avenues to tailor the amount and distribution of Ti species in titanosilicate zeolites to achieve high catalytic performances in various processes.
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Environmental Progress & Sustainable Energy
·
2019
·Vol. 39
·期 4
·e13346
·
IF 1.596
Chunsheng Li,Dandan Liu,Xianqing Yang,Shengjun Chen,Jianchao Deng,Hui Rong,Chi Li
Biodegradation by microorganisms is a potential treatment technique of malachite green (MG) in aquaculture environments. In this study, a bacterium with high MG decolorization and biodegradation ability was isolated through enrichment culture and color-decoloration circle method, and was identified as Klebsiella pneumoniae WA-1 after molecular and biochemical identification. The MG biodegradation characteristics and the factors affecting the MG biodegradation ability of this strain were studied using KBH 4 reduction-high performance liquid chromatography with fluorescence detector (HPLC-FLD) method. K. pneumoniae WA-1 could efficiently biodegrade MG in aqueous solution without any nutrients that over 94.0% MG was biodegraded by this strain at 1–20 mg/L within 0.5 hr and 100% MG were biodegraded at 1–10 mg/L within 12 hr. Fairly stable MG biodegradation performance was observed in complex environments, including various pH, temperature, salinity, and metal ions, especially at low MG concentration. Furthermore, the presence of NaCl, Mn 2+ , Mg 2+ , or Cu 2+ could enhance the MG biodegradation ability of this strain. These results indicate that K. pneumoniae WA-1 could be used as a potential MG degradation agent in complex aquaculture environments.
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BIOORGANIC CHEMISTRY
·
2019
·
IF 3.926
Zhiyan Li,Zhenguo Zhang,Huiyong Sun,Lili Xu,Cheng Jiang
A series of new peptidomimetics targeting the polo-box domain (PBD) of polo-like kinase 1 (Plk1) was identified based on the potent and selective pentapeptide Plk1 PBD inhibitor PLHSpT. Unnatural amino acid residues were introduced to the newly designed compound and the N -terminal substituent of the peptidomimetic was investigated. The optimized compound 9 inhibited the Plk1 PBD with IC 50 of 0.267 μM and showed almost no inhibition to Plk2 PBD or Plk3 PBD at 100 μM. Biolayer interferometry studies demonstrated that compound 9 showed potent binding affinity to Plk1 with a K d value of 0.164 μM, while no K d were detected against Plk2 and Plk3. Compound 9 showed improved stability in rat plasma compared to PLHSpT. Binding mode analysis was performed and in agreement with the observed experimental results. There are only two natural amino acids remained in the chemical structure of 9 . This study may provide new information for further research on Plk1 PBD inhibitors.
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Journal of the Iranian Chemical Society
·
2019
·Vol. 16
·期 12
·2743-2754
·
IF 1.742
Huang Yunfang,Xu Hui,Luo Dan,Guo Qiyao,Zhao Yuezhu,Fang Yu,Wei Yuelin,Fan Leqing,Wu Jihuai
In this work, a series of flower-like Bi/BiOCl x Br (1− x ) heterojunction photocatalysts have been developed. Bi nanoparticles were grown on the BiOCl x Br (1− x ) nanosheets via an in situ chemical reduction with the assistance of cetyltrimethylammonium chloride and cetyltrimethylammonium bromide aqueous solution. The in situ growth of Bi nanoparticles on the BiOCl x Br (1− x ) nanosheets could not only give rise to the optical absorption in the visible region, but also promote the photocatalytic performance of BiOCl x Br (1− x ) . Bi/BiOCl 0.8 Br 0.2 exhibited the highest photocatalytic performance, which could completely degrade RhB in 12 min under the UV light irradiation and 6 min under visible light irradiation, respectively. Moreover, holes and superoxide radicals were verified to be the primarily active species in the photocatalytic process.
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JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
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2019
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IF 2.606
Da Wang,Qinfu Zhao,Tongying Jiang,Luping Sha,Siling Wang,Yongbo Song
Glutamic acid modified Pluronic P123 (P123-G) was synthesized to coat hydrophobic mesoporous silica nanoparticles (PGMSN) aiming to target large amino acid transporter 1 (LAT1) overexpressed cancer cells. We discovered that, once internalized, PGMSN could be transported out of the cells with their cargo, and the exported nanoparticles were then taken up by neighboring cells. This intercellular delivery of particles led to a deeper tumor penetration, presumably through tandem cycles of LAT1 mediated endocytosis and exocytosis. Taking advantage of high drug loading capability of MSN, a kind of cyanine dye Cypate acting as a photothermal converting agent was loaded into MSN along with the chemotherapeutic drug doxorubicin hydrochloride (DOX) to improve the anti-cancer effect by the combination of chemotherapy and photothermal therapy (PTT). Both DOX and Cypate stably dispersed in the pores of MSN, and the coating of P123-G serving as diffusion barrier blocked drug preleakage. The system possessed pH and near-infrared (NIR) light dual-responsive DOX release property. Additionally, PGMSN exhibited targeted PTT effect which strengthened cytotoxic activity of the system due to the enhanced endocytosis. So the deep tumor penetration and targeted PTT effect made PGMSN especially suitable for cancers overexpressing LAT1 receptors.
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