JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
·
2018
·Vol. 18
·期 1
·576-582
·
IF 1.354
Yu Yadong,Wu Na,Peng Gang,Li Tao,Jiang Ling,Huang He
Convenient and sensitive methods for the detection of α-ketoglutarate (α-KG) will be beneficial for biomedical diagnosis and microbial fermentation industry. In this work, reduced MoS2 nanoparticles (rMNs) were prepared by ultrasonication and electrochemical reduction.
The rMNs were then decorated with gold nanoparticles (Aunano) to fabricate rMNs-Aunano nanocomposite. The glass carbon electrode modified with rMNs-Aunano nanocomposite (rMNs-Aunano-GCE) showed excellent electro-catalytic oxidation toward NADH with
a satisfied reproducibility and exhibited linear response in the range of 38.46–214.29 μM. Moreover, the L-Glutamic dehydrogenase modified rMNs-Aunano-GCE exhibited a sensitive and fast response to α-KG, with a linear response range of 11.12–52.94 μM
and a detection limit of 6.25 μM. These findings will pave ways for the α-KG detection and the application of MoS2 or other two-dimensional materials in electrochemical sensing.
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JOURNAL OF MATERIALS ENGINEERING AND PERFORMANCE
·
2018
·Vol. 27
·期 4
·1927-1935
·
IF 1.34
Zhang Xiaosheng,Xu Hong,Dong Jinxiang
Nickel-ion-exchanged α -zirconium phosphate (Ni- α -ZrP) was synthesized by a mild hydrothermal synthesis method. Different raw material ratios (NaF/H 3 PO 4 /Ni(CH 3 COO) 2 ·4H 2 O) influence the particle size of the Ni- α -ZrP samples. The grain size could be controlled and distributed from 20 to 600 nm. Ni- α -ZrP was evaluated as an additive in lithium grease in a four-ball test. A 3.0 wt.% addition of Ni- α -ZrP to lithium grease yielded maximum non-seizure load values of 1235 N, and the wear scar diameter on the lower balls is 0.42 mm at 294 N. Compared with smaller particles, the addition of Ni- α -ZrP with a larger particle size to grease yields a better load-carrying capacity.
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CATALYSIS TODAY
·
2015
·
IF 3.893
Faheem Nawaz,Yongbing Xie,Hongbin Cao,Jiadong Xiao,YueqiuWang,Xihua Zhang,Mingjie Li,Feng Duan
Two series of α-MnO 2 materials were prepared via a similar method with surfactant cetyltrimethylammonium bromide (CTAB) and sodium dodecyl benzene sulfate (SDBS). The surfactants work both as shape directing agent and reduction initiator. The α-MnO 2 synthesized with 0.2 M of CTAB showed a mesoporous structure with high surface area of 387.7 m 2 /g. It is very active in catalytic ozonation of 4-nitrophenol (4-NP) and total organic carbon removal, with higher catalytic stability than commercial MnO 2 . Using tert -butanol and p -benzoquinone as scavengers of different oxidative species, we found that superoxide radicals, rather than hydroxyl radicals, were mainly responsible for the degradation of 4-NP.
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Chemical & Biomedical Imaging
·
2023
·
IF 0
Hanbo Song,Mengxin Xu,Jie Cai,Junyi Chen,Yu Liu,Qi Su,Zhu Li,Zhibo Liu
Targeted alpha therapy (TAT) is an innovative approach in cancer treatment that aims to selectively deliver high-energy radiation to cancer cells while minimizing damage to healthy tissues. 225Ac, an alpha radionuclide with a half-life of 10 days and emitting 4 alpha particles and 2 beta particles in its decay chain, has shown promise for TAT. Fibroblast activation protein (FAP) has emerged as a valuable target for the development of radiopharmaceuticals in the context of TAT, given its expression in various cancers. However, a challenge arises when using FAP-targeting agents such as FAP inhibitors (FAPIs) in combination with 225Ac due to their rapid clearance from the tumor. To address this challenge, the FAP-targeting antibody PKU525 was developed as a TAT drug. The conjugation ratios of the chelator and the labeling procedure were systematically investigated, resulting in an efficient and stable radiolabeling method. Biodistribution studies were conducted using [225Ac]Ac-DOTA-PKU525 with average drug-to-antibody ratios (DARs) of 1.85, 3.87, and 7.72. A single dose of 11.1 kBq of [225Ac]Ac-DOTA-PKU525 with DAR 3.87 demonstrated significant inhibition of 4T1-hFAP tumor growth. No significant weight changes were observed throughout the treatment period, and histological examination of the major organs revealed no adverse side effects. In conclusion, [225Ac]Ac-DOTA-PKU525 exhibited both safety and efficacy in 4T1-hFAP tumor-bearing mice, indicating its potential for clinical translation in FAP-targeted alpha therapy. Further development and evaluation of this TAT approach hold promise for improving cancer treatment outcomes.
研究领域:
肿瘤学
·
核医学
·
放射治疗
·
生物制药
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DOI
iScience
·
2022
·
IF 6.107
Wei Shi,Chunhui Li,Haiqing Wang,Zhiquan Wang,Lei Yang
Owing to the characteristics of THz wave, the terahertz time-domain spectral (THz-TDS) system has potentials in the field of biological macromolecule detection. However, water with strong absorption effect on THz wave exists in most biological detection, so the research focus in this field is to study aqueous samples. In view of these, THz spectroscopy system has research value for qualitative and quantitative detection of α-lactose and its water-containing samples. This research used a THz-TDS system with LiNbO 3 crystal to generate strong THz wave that was used to test 0.29 mmol α-lactose samples with water content of 15 μL–930 μL by using attenuating total reflection (ATR) prism. The absorption peak at 0.53 THz is detected, and with the increase of water content, the curve of absorption spectrum is observed to move up on the whole. This research has a guiding role for the test and improvement of water content limit in this field.
研究领域:
生物物理学
·
分析化学
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Biomass Conversion and Biorefinery
·
2020
·Vol. 11
·期 3
·795-801
·
IF 2.602
Huang Jingang,Guo Kangyin,Shi Binfang,Li Jianping
Henna ( Lawsonia inermis ) plant biomass is a popular source of natural dye lawsone used all over the world. In this study, the effect of temperature on fermentative production of short-chain fatty acids (SCFA) from raw henna biomass was investigated. The results indicated that mesophilic fermentation (35 °C) upgraded the SCFA production as compared with those under normal (25 °C) and thermophilic (25 °C) conditions, increasing by up to 2.2-fold and 1.5-fold, respectively. Acetic acid was the main SCFA component, accounting for 50 ~ 70% of the total SCFA, and followed by n -butyric acid and propionic acid. The enzymatic activities of α-glucosidase in thermophilic series were lower than those in mesophilic series, resulting in a less carbohydrate consumption and SCFA production. Soluble lawsone was positively correlated with fermentation temperature; and the average concentration (87.0 mg/L) in mesophilic series was appropriate to serve as a redox mediator to improve the acidification process for SCFA production. Future work to systematically scrutinize the confounding factors affecting the fermentation process of henna biomass was required.
Graphical abstract
研究领域:
生物工程
·
环境科学
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Korean Journal of Pain
·
2024
·
IF 3.4
Kaiwen Lin,Datian Fu,Zhongtao Wang,Xueer Zhang,Canyang Zhu
Background Galangin, commonly employed in traditional Chinese medicine for its diverse medicinal properties, exhibits potential in treating inflammatory pain. Nevertheless, its mechanism of action remains unclear. Methods Mice were randomly divided into 4 groups for 7 days a normal control group, a galangin-treated (25 and 50 mg/kg), and a positive control celecoxib (20 mg/kg). Analgesic and anti-inflammatory effects were evaluated using a hot plate test, acetic acid-induced writhing test, acetic acid-induced vascular permeability test, formalin-induced paw licking test, and carrageenan-induced paw swelling test. The interplay between galangin, transient receptor potential vanilloid 1 (TRPV1), NF-κB, COX-2, and TNF-α proteins was evaluated via molecular docking. COX-2, PGE2, IL-1β, IL-6, and TNF-α levels in serum were measured using ELISA after capsaicin administration (200 nmol/L). TRPV1 expression in the dorsal root ganglion was analyzed by Western blot. The quantities of substance P (SP) and calcitonin gene-related peptide (CGRP) were assessed using qPCR. Results Galangin reduced hot plate-induced licking latency, acetic acid-induced contortions, carrageenan-triggered foot inflammation, and capillary permeability in mice. It exhibited favorable affinity towards TRPV1, NF-κB, COX-2, and TNF-α, resulting in decreased levels of COX-2, PGE2, IL-1β, IL-6, and TNF-α in serum following capsaicin stimulation. Galangin effectively suppressed the upregulation of TRPV1 protein and associated receptor neuropeptides CGRP and SP mRNA, while concurrently inhibiting the expression of NF-κB, TNF-α, COX-2, and PGE2 mRNA. Conclusions Galangin exerts its anti-inflammatory pain effects by inhibiting TRPV1 activation and regulating COX-2, NF-κB/TNF-α expression, providing evidence for the use of galangin in the management of inflammatory pain.
研究领域:
药理学
·
生物化学
·
分子生物学
·
炎症研究
·
疼痛管理
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2024
·Vol. 59
·期 9
·6462-6472
·
IF 2.6
Tian Siyi,Chang Guoli,Xiang Yannan,Cai Chenggang,Luo Xinyu,Zhu Ruiyu,Yang Hailong,Gao Haiyan
In order to investigate the hypoglycaemic mechanism and potential applications of four hypoglycaemic flavonoid glycosides, namely myricitrin, Cyanidin-3-O-glucoside (C3G), hyperoside and quercitrin in Myrica rubra pomace, the stability of these four flavonoid glycosides and their binding mechanisms were studied using molecular docking. The results demonstrated that pH value affects on the stability of these four components in M. rubra pomace. C3G exhibited the most significant inhibitory effect on α-glucosidase at pH 5, with myricitrin, hyperoside and quercitrin showing the highest inhibitory effect at pH 7. Moreover, an increase in temperature and storage time reduced the inhibitory effect of these four glycosidic components on α-glucosidase. Molecular docking analysis revealed that myricitrin formed hydrogen bonds with the active site residues of α-glucosidase, namely Phe550, Ile552, Asp555, Ser574 and Arg576, and also engaged in hydrophobic interactions with Lys551. Hyperoside formed hydrogen bonds with α-glucosidase, formed hydrophobic interactions with Lys50 and exhibited π-cation interaction with Lys53. Quercitrin formed hydrogen bonds with α-glucosidase, formed hydrophobic interactions with Lys500 and established salt bridges with Lys50. C3G formed hydrogen bonds and hydrophobic interactions with α-glucosidase and showed π-π interactions with Phe301. These findings will provide valuable insights for the application of these four chemicals.
研究领域:
食品科学
·
药理学
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Journal of Materials Chemistry B
·
2025
·
IF 5.7
Yu Li,Pei Xie,Ruixin Gong,Junlong Chen,Yaxue Liu,Zongning Yin
Rheumatoid arthritis (RA), a chronic autoimmune disorder affecting 1% globally, urgently demands advanced therapies to overcome the systemic toxicity and limited efficacy of conventional glucocorticoids like dexamethasone (Dex). In this study, we constructed LMWH-Gly-Pro-ODA/Dex (LGPO/Dex) micelles, which synergistically integrate low molecular weight heparin (LMWH)-mediated active targeting with Gly-Pro-mediated fibroblast-activated protein (FAP)-α-responsive drug release to achieve spatiotemporal precision in RA treatment. The system operates through a three-stage cascade mechanism: (1) targeting the inflamed joints, (2) inflammation-responsive drug release to modulate pathological microenvironments (e.g., normalizing M1/M2 macrophage polarization), and (3) suppression of monocyte recruitment to prevent disease progression. LGPO/Dex micelles showed excellent RA therapeutic effects in the adjuvant-induced arthritis (AIA) model. Joint swelling, serum TNF-α, and nitric oxide (NO) levels in LGPO/Dex-treated rats showed no significant difference from healthy controls (ns) while exhibiting marked improvement over Dex monotherapy (**, P < 0.01). Notably, it also significantly reduced Dex-associated adverse effects. This study confirmed the feasibility of using FAP-α as a therapeutic target for RA and provided a new idea for RA treatment, offering a blueprint for disease-microenvironment-programmed therapeutics.
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Mohammed Mansour,Xiaoqing Li,Heba Elhadedy,Alsadig Yahya,Mahmoud salah,Ting Wu,Xu Xiaoyun
The present study investigates the effect of carboxymethylation modification on the physiochemical properties, structure characterization and biological properties of Houttuynia cordata polysaccharide (HCP). The successful synthesis of carboxymethylated HCP (C-HCP) was confirmed by the degree of substitution, FTIR and NMR results. The carbohydrate content and uronic acid of C-HCP were decreased compared with native HCP, while C-HCP exhibited higher water solubility and lower viscosity. The molecular weights of HCP and C-HCP were 5.123 × 10 5 Da and 2.581 × 10 5 Da, respectively. Both polysaccharides were contained the same monosaccharide composition with a slight change in the molar ratio. C-HCP exhibited a greater thermal stability than native HCP. SEM and AFM microscopic observation confirmed the changes on the polysaccharide chain after carboxymethylation. C-HCP demonstrated stronger hydroxyl radical scavenging activity than HCP. Both HCP and C-HCP inhibited α-glucosidase . However, C-HCP showed substantially higher activity, with inhibition rates of 84.66 ± 0.44%, compared with 53.68 ± 2.40% for native HCP at a concentration of 10 mg/mL. Furthermore, kinetic analyses revealed that both HCP and C-HCP inhibited α-glycosidase via a mixed-type mechanism. Fluorescence quenching and molecular docking studies further demonstrated that C-HCP exhibited a higher binding affinity toward α-glycosidase compared with native HCP. Overall, carboxymethylation effectively enhanced the function and hypoglycemic activity of HCP, suggesting that, C-HCP may serve as a potential therapeutic agent or as functional food to control hypoglycemic.
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Marine Drugs
·
2026
·Vol. 24
·期 2
·84
·
IF 5.7
Wenjie Wang,Kun Gao,Guantian Li,Zongji Wang,Kecheng Li,Song Liu,Huahua Yu,Ronge Xing
Type 2 diabetes mellitus (T2DM) is one of the most prevalent chronic metabolic diseases, and inhibition of α-glucosidase activity represents an effective therapeutic strategy. Chitin is the most abundant renewable polysaccharide in the ocean, with its monosaccharide beingN-acetylglucosamine (NAG). To evaluate the potential of NAG glycosides as novel α-glucosidase inhibitors, three common phenolic compounds were modified via NAG glycosylation. Their inhibitory activities were assessed at both the enzymatic and cellular levels. In addition, density functional theory (DFT), molecular dynamics (MD) simulations, and molecular docking analyses were employed to systematically investigate the effects of NAG glycosylation on enzyme inhibition and the underlying mechanisms. Compared with the parent phenolic compounds, NAG glycosides exhibited significantly enhanced α-glucosidase inhibitory activity, with NAG introduction markedly improving their binding affinity to α-glucosidase. Among them, glycoside3adisplayed the optimal inhibitory effect, comparable to acarbose, and at the cellular level, its activity at high concentrations was comparable to or slightly higher than that of metformin. Circular dichroism (CD) and MD analyses indicated that glycoside3aincreased the conformational flexibility of key residues and enhanced the structural looseness of the enzyme, thereby inhibiting its activity. NAG glycosides constitute a promising class of marine-derived α-glucosidase inhibitors, warranting further structural optimization and rational design to enhance their activity and selectivity.
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PubMed
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Life-Basel
·
2026
·Vol. 16
·期 2
·235
·
IF 3.9
Qingfeng Guo,Xia Zhang,Hao Zhang,Mengxue Wang,Haoran Zhou,Meiru Chen,Zhenhua Yin,Juanjuan Zhang,Baocheng Yang,Li Wang,Lin Chen
Originating in East Asia, the adzuki bean (Vigna angularis) is a diploid crop mainly grown in this region that belongs to the Fabaceae family. As a potential functional food resource with both medicinal and nutritional value, it offers various health benefits. However, research on its chemical constituents, particularly key bioactive components such as triterpenoid saponins and flavonoids, remains relatively limited. In this study, a new triterpenoid saponin, adzukisaponin A (1), along with eleven known compounds (2–12), were isolated from adzuki bean (V. angularis). Among them, compounds3(yunganoside B1),6(3β-acetyl oleanolic acid), and7(β-amyrin) are reported from this source for the first time. Saponins1–3and flavonoid8exhibited significant dual inhibitory activity. Notably, saponins1–3inhibited pancreatic lipase (IC50= 0.11 ± 0.18 to 0.40 ± 0.21 mM) more strongly than the positive control orlistat, while also acting against α-glucosidase (IC50= 0.14 ± 0.11 to 0.23 ± 0.17 mM). Molecular docking supported their binding to the enzymes’ active sites. This study identifies1–3and8as dual α-glucosidase/pancreatic lipase inhibitors, underscoring the potential of adzuki bean as a source of bioactive functional food ingredients.
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危险品化学品经营许可证（带存储）