CARBOHYDRATE POLYMERS
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2012
·
IF 3.628
Jun Huang,Wan-wen Chen,Sheng Hu,Jing-Yan Gong,Hong-Wu Lai,Peng Liu,Le-he Mei,Jian-wei Mao
TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl radical)-mediated 6-carboxy β-chitin derivatives (T-chitin) with different carboxylate content were successfully synthesized by controlling the addition level of NaClO as the primary oxidant. The structural and biochemical properties of the derivatives were investigated. The carboxylate contents of the derivatives calculated by electrical conductivity titration were 1.33, 1.68, 1.80, and 2.08 mmol/g, respectively. The yield of T-chitin with carboxylate content of 2.08 mmol/g reached 74.55%. T-chitin exhibited stronger bile acid binding capacities than that of β-chitin. The scavenging ability of T-chitin against hydroxyl radicals improved with increasing concentration, and EC 50 values were below 1.2 mg/mL. All T-chitin exhibited a strong ferrous ion chelating effect. At 8 mg/mL, the chelating effects of T-chitin with carboxylate content of 0.81 mmol/g reached 80.15%. These results showed that T-chitin had good bile acid binding capacity and antioxidant activities and it may be a potential antioxidant in vitro .
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CERAMICS INTERNATIONAL
·
2025
·
IF 5.6
Renping Cao,Zhiyuan Li,Jingheng Nie,Xiaochun Li,Lei Zhang,Ruirui Yang,Hui Ao,Jingjing Duan,Chenxing Liao
Near-infrared (NIR) luminescent materials based on Fe 3+ are growing interest in recent years because of the low-toxicity, high-efficiency, and adjustable emission. In this work, a broadband NIR emitting β-Mg 3 Ga 2 GeO 8 : Fe 3+ with [Ga/GeO 4 ] tetrahedron for the replacement of Fe 3+ ion is synthesized in air. X-ray diffraction patterns are used to confirm the crystal structure and phase purity. The excitation spectrum is observed in the region from 210 to 550 nm due to the O 2− - Fe 3+ charge transfer and the d – d electron transitions in 3 d 5 orbit of Fe 3+ . A broadband NIR emission of β-Mg 3 Ga 2 GeO 8 : Fe 3+ with full width at half maximum (∼108 nm) in the region from 610 to 880 nm is assigned to the transition 4 T 1 ( 4 G) → 6 A 1 ( 6 S) of Fe 3+ ion. Photoluminescence (PL) spectra under different excitation wavelengths, concentration dependent PL spectra, time-resolved PL spectra, temperature dependent PL spectra, and lifetimes of β-Mg 3 Ga 2 GeO 8 : Fe 3+ are investigated. We confirm the luminous center (Fe 3+ ), the optimal Fe 3+ concentration (0.8 mol%), and the good thermal stability of β-Mg 3 Ga 2 GeO 8 : Fe 3+ . The luminescence mechanism is analyzed theoretically by the Tanabe-Sugano and energy level diagrams of Fe 3+ in β-Mg 3 Ga 2 GeO 8 : Fe 3+ , and the concentration and thermal quenching mechanisms are explained. The research results will be conducive to the development of new Fe 3+ -doped luminescence materials.
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CHEMISTRY OF MATERIALS
·
2025
·
IF 7
Jia Kong,Jiaxing Zhang,Yirui Zhang,Huanxin Xue,Xin Guo,Nan Wang,Ziyi Gao,Menghuan Sun,Yinqiang Xia,Yuefei Wang,Hao Ren,Peng Yang,Man Shing Wong
Amyloid β (Aβ) aggregates are a neuropathological hallmark of Alzheimer’s disease (AD). Despite being a prime therapeutic target, past Aβ-targeted trials have primarily focused on its hydrophobic core, yielding unsatisfactory results. In this study, we aligned the structural features of Aβ42 filaments and found that the hydrophobic core and C-terminal region show notable similarities, and the N-terminal region exhibits considerable disorder. Through residue substitution studies, we identified the His13-His14 motif at the disorder–order boundary as particularly effective in mitigating Aβ42’s fibrillation pathway and neurotoxicity, alongside the Phe19-Phe20 hydrophobic core, presenting a promising target for therapeutic intervention. We then designed a series of peptide mimetics based on these Aβ features. Among them, peptide mimetic III, with the sequence of Phe-His-His, exhibited an assembly of antiparallel β-sheet structures primarily driven by hydrogen bonding, showcasing favorable rheological properties and biosafety. Interestingly, mimetic III predominantly patches the N-terminal region of Aβ42 fibrils through hydrogen bond-dominated interactions, effectively modulating aggregation and neurotoxicity in a “like-interacts-with-like” manner. Furthermore, mimetic III eliminates the risk of Aβ42 aggregating into neurotoxic intermediates, rescuing both human and mouse-derived neuronal cells from Aβ42-induced toxicity. Our study demonstrated that peptide mimetics targeting the N-terminal region of Aβ42 is a promising alternative for modulating amyloidosis-related neurotoxicity.
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npj Clean Water
·
2025
·
IF 11.4
Lv Shiyu,Yuan Tian,Zhang Xueyan,Yang Pu,Fu Kerong,Yao Xia,Fu Weilin,Zhang Qiuxian,Luo Yanli,Wang Feng
Herein, LaFeO 3 photocatalysts (HX-LFO and SX-LFO) were synthesized using hydrothermal and sol–gel methods, respectively, for removing β-E2 from dairy cattle breeding wastewater. A combination of flocculation pretreatment and photocatalytic Fenton oxidation was used to achieve efficient β-E2 degradation. Results revealed that HX-LFO had high defect concentrations and achieved better photocatalytic Fenton oxidation efficiency for β-E2 than SX-LFO. The H0.7-LFO/H 2 O 2 /visible (Vis) system completely degraded 2 mg/L of β-E2 within 2 h and achieved a total organic carbon (TOC) mineralization rate of 99.7%. The intermediate products, degradation pathways, and ecological toxicity of β-E2 degradation were analyzed. The H0.7-LFO/H 2 O 2 /Vis system exhibited stable degradation performance when humic acid and multiple anions coexisted in the reaction solution. The developed system increased the β-E2 degradation rate to 83.38%–100%. Characterizations and density functional theory calculations revealed that the La-defect formation energy in La/O dual-defect LaFeO 3 was lower than that in LaFeO 3 containing only La defects. Moreover, O defects facilitated the formation of La defects that lowered the energy barrier for free-radical desorption, making it easier for free radicals to form and diffuse into the reaction solution. La/O dual-defect LaFeO 3 exhibited lower binding energy for β-E2, accelerating charge transfer within itself and from itself to β-E2.
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CHEMICAL ENGINEERING JOURNAL
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2025
·
IF 13.2
Li-Jun Zhao,Qing Zhou,Shixin Zhang,Li-Xiang Wang,Zi-Wen Guo,Chao Shi,Yanhong Li,Feng Luan,Chunyuan Tian,Yue Cao,Peng Xiao,Xuming Zhuang
High active organometallic catalyst always faced neck in biological sensing due to poor biocompatibility and scarce catalytic triggered signal release reactions, although it has already demonstrated extremely high application potential in biological orthogonal catalytic fields. Here, we firstly showed a novel organoiridium catalyst Ir1@CD by assembling β - cyclodextrin (β-CD) and adamantane functionalized pyridine-amide iridium complexes Ir1 , which was characterized by Two-dimensional rotating-frame overhauser spectroscopy (2D ROSEY) 1 H NMR and Isothermal Titration Calorimetry ITC (bonding constant Ks ~10 5 M −1 ). Over 20.0 % yield increase was obtained when Ir1@CD was used as catalysts compared to Ir1 for trimethyl p-benzoquinone TMBQ hydrogenation, which demonstrated that β-CD could significantly enhanced catalytic activity of Ir1 . Further, a robust biomimetic catalytic hydrogen transfer sensing system was established by combining Ir1@CD with new stable quinone‑hydrogenation-cleavage probes PMNQ . The sensing system presented higher fluorescence enhancement factor (19.7-fold), good linear responses ranged from 1.56 to 50 μM, lower limits of detection LOD (160.0 nM) for cofactor NADH, and could be successfully applied in intracellular imaging of NADH. The provided biocatalytic strategy will also be expected to use in other fields, such as biomimetic organometallic catalysts, drug delivery and many more chemical-biological technologies.
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Frontiers in Pharmacology
·
2021
·
IF 5.811
Ma Liang,Zheng Jiaojiao,Chen Huijing,Zeng Xia,Wang Shilin,Yang Chen,Li Xi,Xiao Yushuo,Zheng Ling,Chen Hong,Huang Kun
The toxic aggregates of amyloid beta (Aβ) disrupt the cell membrane, induce oxidative stress and mitochondrial dysfunction, and eventually lead to Alzheimer’s disease (AD). Intervening with this cytotoxic aggregation process has been suggested as a potential therapeutic approach for AD and other protein misfolding diseases. Traditional Chinese Medicine (TCM) has been used to treat AD and related cognitive impairment for centuries with obvious efficacy. Extracts or active ingredients of TCMs have been reported to inhibit the aggregation and cytotoxicity of Aβ. However, there is a lack of systematic research on the anti-Aβ aggregation effects of TCM components. In this study, we performed a systematic screening to identify the active ingredients of TCM against the cytotoxic aggregation of Aβ42. Through a literature and database survey, we selected 19 TCM herbals frequently used in the treatment of AD, from which 76 major active chemicals without known anti-amyloid effects were further screened. This took place through two rounds of MTT-based screening detection of the cytotoxicity of these chemicals and their effects on Aβ42-induced cytotoxicity, respectively. Tetrahydroxystilbene-2-O-β-D-glucoside (TSG) and sinapic acid (SA) were found to be less toxic, and they inhibited the cytotoxicity of Aβ42. Further studies demonstrated that TSG and SA concentration-dependently attenuated the amyloidosis and membrane disruption ability of Aβ42. Thus, we identified two novel chemicals (TSG and SA) against the cytotoxic aggregation of Aβ42. Nonetheless, further exploration of their therapeutic potential is warranted.
研究领域:
神经科学
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药理学
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传统中药
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FUEL
·
2018
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IF 4.908
Cheng Chen,Dongxue Jin,Xinping Ouyang,Lisha Zhao,Xueqing Qiu,Furong Wang
The structure of lignin is one of the key factors affecting its depolymerization into phenolic monomers . Four lignin fractions with different molecular weight were obtained by using ultrafiltration membrane technology , and the characteristics of lignin factions with different molecular weight were determined by gel permeation chromatography (GPC), potentiometric titration , FT-IR and NMR. It is found that wheat alkali lignin with low molecular weight contained more content of phenolic hydroxyl , methoxyl function groups, and β-O-4 linkages, which was relatively prone to depolymerize into phenolic monomers with a higher yield, although the more content of carbonyl was reported to facilitate the depolymerization of lignin. With the increase of molecular weight of lignin, the depolymerization was more difficult, and the YMC decreased. Lignin fraction with low molecular weight was favorable for depolymerization into syringyl and alkyl syringyl, while the high molecular weight lignin was prone to depolymerize into guaiacyl and alkyl guaiacyl products. Boric acid was proved effective to avoid repolymerization and improve the YMC, and the dosage of boric acid was determined by the content of hydroxyl groups in lignin.
研究领域:
生物化学
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材料科学
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Marine Drugs
·
2020
·Vol. 18
·期 10
·488
·
IF 4.073
Limeng Zhu,Ruilian Li,Siming Jiao,Jinhua Wei,Yalu Yan,Zhuo A. Wang,Jianjun Li,Yuguang Du
It is proven that β-amyloid (Aβ) aggregates containing cross-β-sheet structures led to oxidative stress, neuroinflammation, and neuronal loss via multiple pathways. Therefore, reduction of Aβ neurotoxicity via inhibiting aggregation of Aβ or dissociating toxic Aβ aggregates into nontoxic forms might be effective therapeutic methods for Alzheimer’s disease (AD) treatment. This study was designed to explore interference of chitosan oligosaccharides (COS) on β-(1-42)-amyloid protein (Aβ42) aggregation and Aβ42-induced cytotoxicity. Here it was demonstrated that COS showed good blood-brain barrier (BBB) penetration ability in vitro and in vivo. The experimental results showed that COS efficiently interfered with Aβ42 aggregation in dose- and degree of polymerization (DP)-dependent manners, and COS monomer with DP6 showed the best effect on preventing conformational transition into β-sheet-rich structures. Based on the binding affinity analysis by microscale thermophoresis (MST), it was confirmed that COS could directly bind with Aβ42 in a DP-dependent manner. Our findings demonstrated that different performance of COS monomers with different DPs against Aβ42 assembly was, to some extent, attributable to their different binding capacities with Aβ42. As a result, COS significantly ameliorated Aβ42-induced cytotoxicity. Taken together, our studies would point towards a potential role of COS in treatment of AD.Keywords:Alzheimer’s disease;chitosan oligosaccharides;blood-brain barrier;Aβ42;aggregation;binding affinity;cytotoxicity
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JOURNAL OF SEPARATION SCIENCE
·
2015
·Vol. 38
·期 11
·1813-1821
·
IF 2.737
Qiaoxuan Zhang,Jialiang Guo,Yuan Xiao,Jacques Crommen,Zhengjin Jiang
To further evaluate the feasibility and applicability of the one-pot strategy in monolithic column preparation, two novel β-cyclodextrin-functionalized organic polymeric monoliths were prepared using two β-cyclodextrin derivatives, i.e. mono(6-amino-6-deoxy)-β-cyclodextrin and heptakis(6-amino-6-deoxy)-β-cyclodextrin. In this improved method, mono(6-amino-6-deoxy)-β-cyclodextrin or heptakis(6-amino-6-deoxy)-β-cyclodextrin reacted with glycidyl methacrylate to generate the corresponding functional monomers and were subsequently copolymerized with ethylene dimethacrylate. The polymerization conditions for both monoliths were carefully optimized to obtain satisfactory column performance with respect to column efficiency, reproducibility, permeability, and stability. The obtained poly(glycidyl methacrylate-mono(6-amino-6-deoxy)-β-cyclodextrin- co -ethylene dimethacrylate) and poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin- co -ethylene dimethacrylate) monoliths exhibited a uniform structure, good permeability, and mechanical stability as indicated by scanning electron microscopy and micro-high-performance liquid chromatography experimental results. Because of the probable existence of multi-glycidyl methacrylate linking spacers on the poly(glycidyl methacrylate-heptakis(6-amino-6-deoxy)-β-cyclodextrin- co -ethylene dimethacrylate) monolith, the effect of the ratio of glycidyl methacrylate/heptakis(6-amino-6-deoxy)-β-cyclodextrin was especially studied, and satisfactory reproducibility could still be achieved by strictly controlling the composition of the polymerization mixture. To investigate the effect of the degree of amino substitution of β-cyclodextrin on column performance, a detailed comparison of the two monoliths was also carried out using series of analytes including small peptides and chiral acids. It was found that the β-cyclodextrin-functionalized monolith with mono-glycidyl methacrylate linking spacers demonstrated better chiral separation performance than that with multi-glycidyl methacrylate linking spacers.
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JOURNAL OF MOLECULAR STRUCTURE
·
2024
·
IF 4
Yili Ding,Yanzhi Ma,Charles Ding,Jiehua Nie,Zhe Xu
Through cyclodextrin selection from phase solubility study, complexation method screening, and complexation condition optimization by using single and orthogonal strategy, the complexes of trimethoprim with methyl β -cyclodextrin, HP β -cyclodextrin and HP- γ -cyclodextrin with the best solubility increasing so far were prepared and confirmed by FTIR, DSC and proton NMR spectra. The in vitro pharmacokinetic study showed that trimethoprim was 80 %–90 % released in three complexes in 20 min., and in their dog's in vivo pharmacokinetic study, the C max was increased from 0.22 µg/mL to 0.85 µg/mL, 0.7 µg/mL and 0.72 µg/mL, the AUC 0∼40 was increased from 2.35 µg*h/mL to 3.94 µg*h/mL, 5.06 µg*h/mL and 6.73 µg*h/mL, the LC was shorten from 2.17 L/kg/h to 0.89 L/kg/h, 0.56 L/kg/h and 1.07 L/kg/h for the complexes with methyl β -cyclodextrin, HP β -cyclodextrin and HP- γ -cyclodextrin respectively. After combination with sulfamethoxazole cyclodextrin complex, the antibacterial activity of the complex of trimethoprim with methyl β -cyclodextrin was increased significantly compared with the pure drug alone, indicated that this complex can be used as new potent antibacterial agent.
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JOURNAL OF MOLECULAR LIQUIDS
·
2021
·
IF 6.165
Ruihong Wang,Botian Ding,Guizhao Liang
Cyclodextrins (CDs) can improve the solubility and stability of flavonoids by forming supramolecular complexes. However, the detailed inclusion mechanism has not been fully elucidated. In this study, the binding ability of eight flavonoids with four common CDs (α-, β-, methyl-β-, γ-CD) was investigated. Then, the interaction characteristics of two flavonoids (Dihydromyricetin (DMY) and Naringenin) with M-β-CD were further explored by using phase solubility, ultraviolet, infrared, X-ray diffraction, differential scanning calorimetry, molecular docking and ONIOM calculations. The results indicated that the molecular volume of flavonoids and the cavity size of CDs have distinct effects on the host-guest interaction. DMY and Naringenin form stable supramolecular complexes with M-β-CD mainly through hydrophobic interactions and Van der Waals forces. In addition, the stability of the Naringenin/m-β-CD is higher than that of DMY/M-β-CD, which is consistent with the experimental stability constant. Both of the above flavonoids entered the large hole of the hydrophobic cavity of M-β-CD through one end of the B-ring, which has a significant contribution to the formation of the host-guest hydrogen bonds . Taken together, this study is helpful to further understand the interaction mechanism between CDs and guest molecules, expand the application of CDs, and promote the utilization of natural active compounds.
研究领域:
药剂学
·
分子生物学
·
化学
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Molecular Catalysis
·
2025
·
IF 4.9
Xiaojie Wu,Shibing Wu,Run Wang,Mingyue Chen,Jiang Liu,Xilong Yan,Yang Li,Bowei Wang,Ligong Chen
The planarity of covalent organic frameworks (COFs) is a critical structural parameter for their photocatalytic performance. However, its influence on the efficiency of photocatalytic organic transformations has not been fully investigated. Herein, three β -ketoenamine COFs with different planarity, denoted as TP-EDDA-COF, TP-DATP-COF, and TP-DMTP-COF, were synthesized to explore the influence of planarity on their photocatalytic performance. With the best planarity, TP-EDDA-COF exhibited a superior visible-light absorption range and photogenerated carrier separation and migration efficiency. Therefore, it demonstrated exceptional photocatalytic performance, with conversions reaching 98 % for the dehydrogenation of tetrahydroquinoline (THQ) and 99 % for the oxidative coupling of benzylamine (BA). Furthermore, TP-EDDA-COF also exhibited excellent substrate scope and recyclability. Comprehensive material characterization and theoretical calculations revealed that the enhanced planarity significantly facilitated charge separation and migration, thereby leading to improved photocatalytic efficiency. This study deepens the understanding of structure-activity relationships for COF photocatalysts, and provides a theoretical foundation for the rational design of high-performance photocatalysts for aerobic reactions.
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