BIOSENSORS & BIOELECTRONICS
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2017
·
IF 7.78
Lijun Zhang,Guiheng Wang,Di Wu,Can Xiong,Lei Zheng,Yunsheng Ding,Hongbo Lu,Guobing Zhang,Longzhen Qiu
In this study, an organic electrochemical transistor sensor (OECT) with a molecularly imprinted polymer (MIP)-modified gate electrode was prepared for the detection of ascorbic acid (AA). The combination of the amplification function of an OECT and the selective specificity of MIPs afforded a highly sensitive, selective OECT sensor. Cyclic voltammetry and electrochemical impedance spectroscopy measurements were carried out to monitor the stepwise fabrication of the modified electrodes and the adsorption capacity of the MIP/Au electrodes. Atomic force microscopy was employed for examining the surface morphology of the electrodes. Important detection parameters, pH and detection temperature were optimized. With the change in the relative concentration of AA from 1 μM to 100 μM, the MIP-OECT sensor exhibited a low detection limit of 10 nM (S/N > 3) and a sensitivity of 75.3 μA channel current change per decade under optimal conditions. In addition, the MIP-OECT sensor exhibited excellent specific recognition ability to AA, which prevented the interference from other structurally similar compounds (e.g., aspartic acid , glucose, uric acid , glycine, glutathione , H 2 O 2 ), and common metal ions (K + , Na + , Ca 2+ , Mg 2+ , and Fe 2+ ). In addition, a series of vitamin C beverages were analyzed to demonstrate the feasibility of the MIP-OECT sensor. Using the proposed principle, several other sensors with improved performance can be constructed via the modification of organic electrochemical transistors with appropriate MIP films.
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2017
·
IF 3.012
Jing Zhang,Xin-he Song,Sa Ma,Xue Wang,Wen-chang Wang,Zhi-dong Chen
A sensitive and selective method was developed for the electrochemical determination of dopamine by using a sodium dodecyl benzene sulfonate modified expanded graphite paste electrode (SDBS-EGPE). The SDBS-EGPE was prepared by mixing expanded graphite (EG) self-assembled with sodium dodecyl benzene sulfonate (SDBS) with solid paraffin. SDBS formed a monolayer on EG surface with a high density of negatively charged end. Compared with EGPE, SDBS-EGPE showed improved electrochemical response of dopamine (DA) due to the enhanced accumulation of protonated DA via electrostatic interactions between positively charged DA and negatively charged SDBS. Under optimal experimental conditions, the designed electrode exhibited wide linear responses to DA ranging from 0.5 μmol/L and 500 μmol/L with a low detection limit of 0.027 μmol/L at a signal-to-noise ratio of 3. The SDBS-EGPE showed a good reproducibility, stability and reusability. The SDBS-EGPE showed a high selectivity for DA in the presence of ascorbic acid (UA) and uric acid (AA) due to the electrostatic repulsions between negatively charged UA , AA and SDBS. The proposed electrode was successfully applied in the determination of DA in the commercial dopamine hydrochloride injection sample.
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International Journal of Nanomedicine
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2017
·
IF 4.3
Quan-Quan Zhuang,Zhi-Hang Lin,Yan-Cheng Jiang,Hao-Hua Deng,Shao-Bin He,Li-Ting Su,Xiao-Qiong Shi,Wei Chen
Dendrite-like cobalt selenide nanostructures were synthesized from cobalt and selenium powder precursors by a solvothermal method in anhydrous ethylenediamine. The as-prepared nanocrystalline cobalt selenide was found to possess peroxidase-like activity that could catalyze the reaction of peroxidase substrates in the presence of H2O2. A spectrophotometric method for uric acid (UA) determination was developed based on the nanocrystalline cobalt selenide-catalyzed coupling reaction between N-ethyl-N-(3-sulfopropyl)-3-methylaniline sodium salt and 4-aminoantipyrine (4-AAP) in the presence of H2O2. Under optimum conditions, the absorbance was proportional to the concentration of UA over the range of 2.0–40 μM with a detection limit of 0.5 μM. The applicability of the proposed method has been validated by determination of UA in human serum samples with satisfactory results.
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INTEGRATED FERROELECTRICS
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2016
·
IF 0.375
Dong-Wei Han,Xiu-Lan Zhang,Bing Yu,Hai-Lin Cong,Chun-Bo Mu,Hua Yuan
Three kinds of carboxyfullerenes, La@C82-COOH, C60-COOH and C70-COOH, were used to modify gold electrodes. The electrochemical behavior of dopamine (DA) at the carboxyfullerenes modified electrodes was studied using the cyclic voltammetry (CV) and differential pulse voltammetry (DPV) methods. The C70-COOH modified electrodes performed the best among them. Under the optimal conditions, linear CV and DPV responses to DA concentration from 1 μmol/L to 13 μmol/L were observed, and the detection limit of DA was 1×10−2 μmol/L in the presence of 10 μmol/L uric acid (UA). The practical analytical utilities of the C70-COOH modified electrodes were demonstrated by the determination of DA HCl in the real samples of dopamine hydrochloride injection (DHI). Moreover, the modified electrodes exhibited good reproducibility and sensitivity, demonstrating its feasibility for analytical purpose.
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RSC Advances
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2016
·Vol. 6
·期 48
·42008-42013
·
IF 3.289
Lihua Jin,Zaihua Zhang,Zhihua Zhuang,Zheng Meng,Cong Li,Yehua Shen
One-dimensional (1D) bimetallic nanowire materials have attracted much more attention in electroanalysis because of their unique physical and chemical properties. In this work, we further extended the application field of the PdPt bimetallic alloy nanowires (PdPt BANWs) structure and successfully fabricated a novel PdPt BANWs-based electrochemical sensor for sensitive detection of ascorbic acid (AA). It was found that the PdPt BANWs on the electrode exhibited remarkable electrocatalytic activity toward AA oxidation and could be used for sensitive detection of AA in a wide linear range (0.01–0.97 mM) with a high sensitivity of 467.9 μA mM−1 cm−2. The detection limit is as lower as 0.2 μM (S/N = 3). Moreover, the electrochemical sensor also exhibited good selectivity, reproducibility and stability, and had been successfully applied to detection of AA in serum samples and commercial vitamin C tablets with satisfactory results.
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BIOSENSORS & BIOELECTRONICS
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2016
·
IF 7.476
Shanying Han,Tianyu Du,Hui Jiang,Xuemei Wang
A self-assembly composite of graphene-pyrroloquinoline quinone (PQQ) was fabricated and modified on glassy carbon electrode (GCE) for sensitive detection of nicotinamide adenine dinucleotide (NADH). Chitosan (CTS) was applied to disperse graphene to form a stable robust film on GCE. A synergistic effect between PQQ and graphene was observed during the electrocatalytic oxidation of NADH, with about 260 mV reduction in the oxidation potential and 2.5-fold increase in the oxidation current compared with those on the bare GCE. The electrochemical sensors based on the modified electrodes allowed the detection of NADH with a good linear dependence from 0.32 to 220 µM with a high sensitivity of 0.421 µA µM −1 cm −2 and a low detection limit of 0.16 µM (S/N=3). It could also eliminate the interference of electroactive substances like ascorbic acid (AA), uric acid , and dopamine and its derivatives. The outstanding performances of graphene-PQQ/CTS composite capable of improving the electrical conductivity and accelerating the electron transport suggested its promising applications for design of different graphene based composites used in electrochemical sensing and energy fields.
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JOURNAL OF SOLID STATE ELECTROCHEMISTRY
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2016
·Vol. 20
·期 7
·2037-2044
·
IF 2.327
Yang Shaoming,Zheng Yue,Zhang Xiaorong,Ding Shaoqing,Li Lingling,Zha Wenling
A sensitive molecularly imprinted sensor for L-cysteine (Cys) determination was designed and fabricated by electrodeposition method. The molecularly imprinted polymer (MIP) film was prepared by electrodeposition chitosan (CS) in the presence of Cys after depositing nanoporous gold (np-Au) and copper nanoparticles (CuNPs) onto a glassy carbon electrode (GCE) surface. The morphology and composition of CuNPs/np-Au/GCE were characterized by SEM and energy dispersive spectroscopy (EDS). The MIP/CuNPs/np-Au/GCE sensor was characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The sensor displayed an excellent recognition to Cys upon measuring the variation of the oxidation current of Cys. The current response was linear to the logarithm concentration of the analyte in the range of 0.5 nM–10 μM, with a detection limit of 0.7 × 10 −10 mol⋅L −1 . The sensor showed good selectivity and reproducibility. The prepared sensor was successfully applied to the determination of Cys in bovine serum and sauce of instant noodle samples with satisfactory recoveries ranging from 93.0 to 110.0 %. The desirable performances may be attributed to the synergic catalytic effect and large surface area of CuNPs/np-Au nanocomposites.
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JOURNAL OF NANOPARTICLE RESEARCH
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2016
·Vol. 18
·期 5
·1-9
·
IF 2.101
Liu Fenfen,He Juan,Zeng Mulang,Hao Juan,Guo Qiaohui,Song Yonghai,Wang Li
Abstract In this work, a facile strategy to synthesize Cu–hemin metal-organic frameworks (MOFs) with peroxidase-like activity was reported. The prepared Cu–hemin MOFs were characterized by various techniques such as scanning electron microscopy, transmission electron microscopy, X-ray powder diffraction, Fourier transform infrared spectroscopy, UV–visible absorbance spectra, and so on. The results showed that the prepared Cu–hemin MOFs looked like a ball-flower with an average diameter of 10 μm and provided a large specific surface area. The Cu–hemin MOFs possessing peroxidase-like activity could be used to catalyze the peroxidase substrate of 3,3,5,5-tetramethylbenzidine in the presence of H 2 O 2 , which was employed to detect H 2 O 2 quantitatively with the linear range from 1.0 μM to 1.0 mM and the detection limit was 0.42 μM. Furthermore, with the additional help of glucose oxidase, a sensitive and selective method to detect glucose was developed by using the Cu–hemin MOFs as catalyst and the linear range was from 10.0 μM to 3.0 mM and the detection limit was 6.9 μM. This work informs researchers of the advantages of MOFs for preparing biomimetic catalysts and extends the functionality of MOFs for biosensor application. Graphical Abstract
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MICROCHIMICA ACTA
·
2016
·Vol. 183
·期 6
·2039-2046
·
IF 4.831
Mei Li-Ping,Feng Jiu-Ju,Wu Liang,Chen Jian-Rong,Shen Liguo,Xie Yunlong,Wang Ai-Jun
Porous cuprous oxide nanospheres were deposited on reduced graphene oxide (pCu 2 O NS-rGO) by a solvothermal approach that uses hexadecyltrimethylammonium bromide as the capping agent and L -glutamic acid as the reducing agent. The nanomaterial was characterized by transmission electron microscopy, Raman spectroscopy, thermogravimetry, and electrochemical methods. A glassy carbon electrode was modified with pCu 2 O NS-rGO, and the respective electrode displays a well expressed oxidation peak for dopamine (DA) located at 160 mV (vs. SCE). It also gives a strong peak for uric acid (UA) which is separated from the DA peak by 130 mV (vs. SCE). No signals can be detected for ascorbic acid (AA) in concentrations up to 2.0 mM. The findings are exploited in a method for simultaneous determination of UA and DA. The linear ranges are from 1.0 to 138 μM for UA, and from 0.05 to 109 μM for DA even in the presence of relatively high concentrations of AA. The detection limits are 112 nM for UA and 15 nM for DA (at an S/N ratio of 3).
Porous cuprous oxide nanospheres deposited on reduced graphene oxide was fabricated by a one-pot solvothermal method. The nanocomposites were explored to construct a sensor for simultaneous determination of uric acid and dopamine in the presence of ascorbic acid with high concentration.
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CHEMISTRY-A EUROPEAN JOURNAL
·
2017
·Vol. 23
·期 64
·16179-16183
·
IF 5.317
Zao Wang,Fengyu Xie,Zhiang Liu,Gu Du,Abdullah M. Asiri,Xuping Sun
Graphical A nickel borate nanoarray on carbon cloth (Ni−Bi/CC) behaves as an efficient catalyst electrode for H 2 O 2 electroreduction in neutral media. As a non-enzymatic electrochemical H 2 O 2 sensor, such Ni−Bi/CC shows superior sensing performances with a fast response time (less than 3 s), a low detection limit (0.85 n m , S / N =3), and a high sensitivity (18320 μA m m cm −2 ). It is highly attractive to construct natural enzyme-free nanoarray architecture as a 3D catalyst for hydrogen peroxide detection due to its great specific surface area and easy accessibility to target molecules. In this communication, we demonstrate that nickel borate nanoarray supported on carbon cloth (Ni−Bi/CC) behaves as an efficient catalyst electrode for H 2 O 2 electro-reduction in neutral media. As a non-enzymatic electrochemical H 2 O 2 sensor, such Ni−Bi/CC shows superior sensing performances with a fast response time (less than 3 s), a low detection limit (0.85 n m , S/ N =3), and a high sensitivity (18320 μA m m cm −2 ). Importantly, it also demonstrates favourable reproducibility and long-term stability.
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BIOSENSORS & BIOELECTRONICS
·
2017
·
IF 7.78
Jianping Li,Xionghui Ma,Mengxi Li,Yun Zhang
A novel glycoprotein imprinting strategy was proposed and was applied to the detection of the carbohydrate antigen 19-9. The glycosylated complex of glycoprotein was used as template to construct a glycosyl imprinted sensor. The eluted imprinted cavities showed good affinity for template glycosyls and glycoproteins carrying template glycosyl. The effect of template saccharide structure on glycosyl imprinted sensors is further discussed. More complex template structures can lead to better sensor performance including selectivity and sensitivity. As a result, the polysaccharide imprinted sensor showed preeminent linear response to CA19-9 in the range of 0.1–5 U/mL, with a detection limit of 0.028 U/mL (3δ/K), while the linear of the monosaccharide imprinted sensor was 1–60 U/mL and the detection limit was 0.17 U/mL. The complex structure on the template surface provides more possibilities for the recognition of the template molecules, consequently, led to the significant anti-interference capability of the polysaccharide imprinted sensor. Furthermore, recoveries ranging from 93.0% to 103.5% were achieved when human serum samples were assayed using the polysaccharide imprinted sensor.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Zhitao Chen,Yue Lin,Xiaoming Ma,Longhua Guo,Bin Qiu,Guonan Chen,Zhenyu Lin
Fish product is one of the main seafood with the disadvantage of easy to perish. Various indexes and diverse methods have been developed to evaluate the fish freshness, but most of them are complex and cannot be applied for on-field detection. In this study, we reported a simple, visual and economical multicolor biosensor based on the etching of gold nanorods (GNRs) to evaluate the fish freshness with the naked eye. Hypoxanthine (Hx) is chosen as the index of fish freshness and it react with the dissolved oxygen to produce H 2 O 2 at the presence of xanthine oxidase (XOD). Then GNRs are etched quickly by the H 2 O 2 in the presence of Fe 2+ . Correspondingly, the GNRs surface plasmon resonance (SPR) is regulated and results in a distinctly color change of the system, which can be easily distinguished with the naked eye. Therefore, the concentration of Hx in the fish samples can be semi-quantitatively analyzed with the naked eye. The proposed multicolor Hx sensor has been successfully applied to the detection of Hx in fish samples.
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