Translational Research
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2024
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IF 6.4
Aoran Huang,Jianyun Rao,Xin Feng,Xingru Li,Tianhua Xu,Li Yao
Vascular calcification (VC) poses significant challenges in cardiovascular health. This study employs single-cell transcriptome sequencing to dissect cellular dynamics in this process. We identify distinct cell subgroups, notably in vascular smooth muscle cells (VSMCs), and observe differences between calcified atherosclerotic cores and adjacent regions. Further exploration reveals ID3 as a key gene regulating VSMC function. In vitro experiments demonstrate ID3′s interaction with USP1 and E12, modulating cell proliferation and osteogenic differentiation. Animal models confirm the critical role of the USP1/ID3/E12/P21 axis in VC. This study sheds light on a novel regulatory mechanism, offering potential therapeutic targets. 1. This study uses single-cell transcriptome to investigate vascular calcification in depth. 2. This study successfully identifies cellular heterogeneity related to vascular calcification. 3. This study reveals the crucial role of ID3 in vascular smooth muscle cell osteogenic differentiation. 4. In vivo and in vitro experiments of this study demonstrate the regulatory role of the USP1/ID3/E12/P21 axis in calcification. 5. This study provides potential molecular targets for the treatment of calcification-related diseases.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Shan-Cheng Yi,Juan Wu,Jia-Qing Wang,Xin-Hui Chen,Man-Qun Wang
Odorant binding proteins (OBPs) are involved in odorant discrimination and act as the first filter in the peripheral olfactory system. Previous studies have shown that BhorOBP29 is potentially involved in olfactory perception in an important wood-boring pest Batocera horsfieldi (Hope) (Coleoptera: Cerambycidae), however, its function remains unclear. Here, we investigated the ligand-binding profiles of recombinant BhorOBP29 with 22 compounds from its host plant using fluorescence competitive binding assays and fluorescence quenching assays. The results showed that BhorOBP29 could bind to five ligands relying mainly on hydrophobic interactions. Molecular docking analysis indicated that residues Ile48, Leu51, Met52, Trp57, Asn105, and Val119 were extensively involved in the interactions between BhorOBP29 and the five ligands. Furthermore, the site-directed mutagenesis analysis revealed that Leu51 and Met52 residues were indispensable for BhorOBP29-ligands binding. Finally, electroantennogram (EAG) assays confirmed that hexanal, (−)-limonene, and 2-methylbutyraldehyde elicited a concentration-dependent EAG response with a maximum at the concentration of 1/10 v /v. These findings suggest that BhorOBP29 may play a significant role in the perception of host plant volatiles by B. horsfieldi . This study may help to discover novel behavioral regulation and environmentally friendly strategies for controlling B. horsfieldi in the future.
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RSC Advances
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2024
·Vol. 14
·期 22
·15240-15248
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IF 3.9
Yuanhui Liu,Guocheng Jin,Han Miao,Shiai Xu
Cardiovascular diseases pose a significant global health threat, and stents play a crucial role in managing these diseases. However, challenges exist with respect to the poor adhesion of stent coatings. Cardiac stents are often composed of titanium–nickel (TiNi) alloys as the metallic component and poly(n-butyl methacrylate) (PBMA) as the coating. The poor adhesion of PBMA to TiNi alloy surface may cause detachment and subsequent thrombosis post-implantation. This study utilizes Reversible Addition-Fragmentation Chain Transfer (RAFT) polymerization to synthesize a novel block copolymer, PBMA-b-PVP, composed of PBMA and poly(N-vinylpyrrolidone) (PVP). TiNi alloy surfaces are functionalized with polydopamine (PDA) to enhance polymer coating adhesion. PBMA-b-PVP exhibits a remarkable improvement in adhesion from class 5 to class 0 and high coating stability after a 15 days immersion in a phosphate buffer solution. The corrosion current density is reduced by 44% with the incorporation of PDA into PBMA-b-PVP coatings, suggesting high corrosion resistance. PDA-functionalized coatings promote cell viability without cytotoxicity, suggesting high biocompatibility. This study provides a robust strategy for preparing stent coatings with high adhesion, corrosion resistance, and biocompatibility.
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MICROPOROUS AND MESOPOROUS MATERIALS
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2024
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IF 4.8
Yuxin Li,Juanping Zhou,Hu Wen,Haitao Yin,Shunyu Han,Nanzhe Jiang
Clay is a rich aluminosilicate resource with unique advantages as a raw material for zeolites production. However, synthesizing nanosized single-crystalline zeolites through a simple approach using clay remains a significant challenge. In this paper, acid-washed montmorillonite (MSR) was used to prepare nano-H-ZSM-5 single crystals with high crystallinity, uniform grain size and permeable straight pores under the induction of a small amount of organic template tetrapropylammonium hydroxide (TPAOH). Through temperature-staged treatment, the nucleation and growth process of zeolite were decoupled, ultimately achieving precise control over the size of the nano-single crystals. Mechanistic study showed that the low-temperature aging process produces smaller structural units which probably derived from the geopolymer topology of the clay, providing more active sites for zeolite nucleation, leading to the rapid crystallization during the high-temperature process. The porous structure promotes material diffusion, inhibits aromatic cycling and reduces carbon formation in the pores. The large specific surface area and nanoscale multilayer pore structure delay carbon accumulation and increase carbon holding capacity. These properties make nano-ZSM-5 single crystals an excellent choice for high olefin selectivity and long catalytic life in the methanol-to-olefin reaction. This synthetic route provides a general strategy for preparing nanosized zeolite single crystals from clay.
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ANALYTICA CHIMICA ACTA
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2024
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IF 5.7
Jianhui Xie,Shuying Mao,Yanglan Zhao,Guimin Zhang,Jingchun Yao,Yongxia Guan,Jizhong Yan,Hui Zhang
Natural products-based screening of active ingredients and their interactions with target proteins is an important ways to discover new drugs. Assessing the binding capacity of target proteins, particularly when multiple components are involved, presents a significant challenge for sensors. As far as we know, there is currently no sensor that can accomplish high-throughput quantitative analysis of natural product-target protein binding capacity based on Raman spectroscopy. In this study, a novel sensor model has been developed for the quantitative analysis of binding capacity based on Surface-Enhanced Raman Spectroscopy (SERS) and Photocrosslinked Molecular Probe (PCMP) technology. This sensor, named SERS-PCMP, leverages the high throughput of molecular probe technology to investigate the active ingredients in natural products, along with the application of SERS labelling technology for target proteins. Thus it significantly improves the efficiency and accuracy of target protein identification. Based on the novel strategy, quantitative analysis of the binding capacity of 20 components from Shenqi Jiangtang Granules (SJG) to α-Glucosidase were completed. Ultimately, the binding capacity of these active ingredients was ranked based on the detected Raman Intensity. The compounds with higher binding capacity were Astragaloside IV (Intensity, 138.17), Ginsenoside Rh2 (Intensity, 87.46), Ginsenoside Rg3 (Intensity, 73.92) and Ginsenoside Rh1 (Intensity, 64.37), which all exceeded the binding capacity of the positive drug Acarbose (Intensity, 28.75). Furthermore, this strategy also performed a high detection sensitivity. The limit of detection for the enzyme using 0.1 mg of molecular probe magnetic nanoparticles (MP MNPs) was determined to be no less than 0.375 μg/mL. SERS-PCMP sensor integrating SERS labeling and photocrosslinked molecular probes which offers a fresh perspective for future drug discovery studies. Such as high-throughput drug screening and the exploration of small molecule-target protein interactions in vitro.
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JOURNAL OF HAZARDOUS MATERIALS
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2025
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IF 11.3
Wenyan Jiang,Shan Zhang,Chen Xu,Haojie Sun,Ziye Xing,Xiao Liang,Hongming He,Quanshun Li
The prevalence of antibiotics in natural aquatic environment has become an emerging class of organic pollutants, impacting both aquatic ecosystems and human health. Herein, spherical mesoporous covalent organic frameworks (SMCOF) was successfully synthesized and employed in the immobilization of OXA-48 β-lactamase through pore adsorption, in which 1,3,5-tris(4-aminophenyl)benzene and 2,5-dimethoxyterephthalaldehyde were utilized as the node and linker, respectively. Structural characterization demonstrated that OXA-48 was successfully adsorbed into the pores of SMCOF, achieving a loading capacity of 546.81 mg/g. Meanwhile, OXA-48@SMCOF maintained the catalytic activity of 62.08 % and 104.98 % after the treatment at 50 °C (pH of 7.4) and 37 °C (pH of 11) for 30 min, respectively. Further, all the catalytic activities of OXA-48@SMCOF remained above 88 % after the incubation in the presence of various organic solvents or proteinase K for 1 h. Moreover, OXA-48@SMCOF retained 53.78 % and 41.18 % of original activity after 15 cycles of reuse and 10 days of storage, respectively, indicating its favorable reusability and storage stability. In the application of degrading antibiotics in lake water samples, OXA-48@SMCOF demonstrated superior degradation performance compared to free OXA-48, attributing to the protective effect of SMCOF. Overall, the present study provides a promising technique to construct high performance immobilized enzymes, which is beneficial for the future application of OXA-48@SMCOF in the degradation of antibiotics during water treatment process.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2025
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IF 5.4
Kangjing Wu,Yunzhen Hu,Shuai Wang,Zhanfang Cao,Xin Ma,Hong Zhong
Solvent extraction is considered as an eco-friendly and efficient technology to extract manganese from an industrial leaching solution based on ores or post-consumer products. However, it is imperative to propose a novel extractant capable of independently and selectively extracting manganese ions from impurities since the separation efficiency of a single extractant is limited. We introduced a novel extractant di-neodecyl ethanedione monoxime (DEM) to extract manganese ions and conducted a comparative extraction analysis with neocapric acid (Versatic 10). The maximum extraction efficiencies of DEM and Versatic 10 separately achieved at 97.34% and 87.62%. And the selectivity tests for the extraction and separation of Mn 2+ and Mg 2+ suggested that DEM exhibited superior selectivity towards manganese ions than Versatic 10. FTIR spectrum analysis, saturation capacity experiments and mass spectrometry suggest that DEM can form a dual-ligand six-membered ring metal complex with manganese or magnesium ions through the formation of coordination bonds of N−O−M and C−O−M (M=Mn 2+ or Mg 2+ ). EDA-NOCV analysis illustrated that the π-backbonding between manganese atoms and extractant ligands contributes to the selectivity separation of manganese and magnesium ions. And the π-backbonding bond between the oxime group (–C=N–OH) and the manganese ion is stronger than that of the carbonyl group (–(C=O)–OH), confirming that DEM demonstrated superior selectivity compared to Versatic 10 in extracting manganese ions from magnesium ions.
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Journal of Inorganic and Organometallic Polymers and Materials
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2025
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IF 4.9
Bai Yizhuo,Zhang Shiyu,Zhang Kaifan,Li Jiali,Zhang Jiehe,Hao Mingxu,Tao Haisheng
Nitro-functionalized NO 2 -MIL-53(Fe) was synthesized by modifying MIL-53(Fe) to enhance persulfate activation for the degradation of tetracycline (TCN). Comparative studies with NH 2 -MIL-53(Fe) and pristine MIL-53(Fe), it was found that NO 2 -MIL-53(Fe) is categorized as an ideal type I isotherm, without observable hysteresis loop. The introduction of nitro-group incorporation significantly increased specific surface area clocking in at 298.3 m 2 /g and an average pore diameter of 3.3 nm, created uniform micropores, and improved adsorption capacity. Structural characterization confirmed Fe(II) generation and electron transfer in NO 2 -MIL-53(Fe), enabling 82.1% TCN removal within 30 min. Radical (SO 4 ·− , ·OH, O 2 ·⁻ ) and non-radical ( 1 O 2 , charge transfer) pathways synergistically drove degradation, as evidenced by quenching experiments and EPR analysis. ESI-Q-TOF MS identified key intermediates, proposing plausible TCN degradation pathways. This study highlights the potential of nitro-functionalized metal-organic frameworks (MOFs) in advanced oxidation processes, emphasizing their enhanced catalytic activity and performance mechanisms, and provides new ideas for the remediation of pollutants in water bodies. Graphical abstract
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APPLIED SURFACE SCIENCE
·
2025
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IF 6.9
Mazhar Khan,Zeeshan Akmal,Muhammad Tayyab,Seemal Mansoor,Muhammad Adnan Zeb,Amir Zeeshan,Muhammad Mubashir Khan,Ziwei Ye,Jinlong Zhang,Shiqun Wu,Lingzhi Wang
The synergistic integration of redox reactions in the photocatalyst poses a significant challenge for CO 2 photoreduction. Addressing this issue requires the design of spatially proximate redox sites while effectively suppressing charge recombination at these sites. This work offers a pioneering tactic that exploits synergistic redox reactions in close atomic proximity. The deliberate incorporation of palladium and indium into TiO 2 , where the Pd site assists in hole accumulation, accelerates water dissociation, and generates highly active H* species. Meanwhile, adjacent In sites capture photogenerated electrons from TiO 2 , producing reactive intermediates by activating CO 2 . The synergistic incorporation of Pd and In accelerates redox functionality, boosts charge carrier separation, and mitigates recombination at closely situated redox sites. Comprehensive characterizations, including in-situ XPS and FTIR, strengthen these innovative outcomes. The optimized Pd 0.5 -In 1 /TiO 2 photocatalyst achieves nearly 100 % selectivity of CH 4 with a production rate of 30.5 µmol g -1 h −1 without the use of any sacrificial reagent, demonstrating a 25-fold improvement compared to pristine TiO 2 . In-situ infrared spectroscopy further validates that the synergistic doping of metal ions promotes the formation of *CH 3 O and *CHO, key intermediates in the production of CH 4 . This investigation provides valuable insight into the intricate design of coupled redox active sites.
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Journal of Energy Storage
·
2025
·
IF 9.8
Zhao Han,Zhihua Zhou,Chendong Wang,Yuwen Zhao,Shifeng Hu,Ruiming Nie
The rapid advancement of renewable energy applications has driven a significant increase in the integration of battery energy storage systems within the construction sector. However, the conversion of electrical energy into heat during charging and discharging cycles can adversely affect battery performance. As building-integrated energy storage systems advance, capturing and reusing waste heat are increasingly recognized as valuable strategies for enhancing building energy efficiency. This study developed a novel flexible composite phase change material (ZES@PW) with a high enthalpy of 162.93 J/g. A battery thermal management model was also established to evaluate the potential of waste heat utilization under varying ambient temperatures and assessed the temperature control and waste heat recovery effectiveness. During the heating season, waste heat recovery led to a 19.61 % to 24.88 % reduction in heating water make-up energy consumption. While in the non-heating season, it reduced domestic hot water heating energy consumption by 2.03 % to 2.42 %. The results confirm that the integration of ZES@PW in thermal management systems not only stabilizes battery temperature but also provides significant energy savings for building heating and domestic hot water systems through the efficient reuse of waste heat.
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PLANT PHYSIOLOGY AND BIOCHEMISTRY
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2025
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IF 5.7
Weijuan Xu,Jianyi Li,Shuguang Jian,Mingyong Zhang,Yongxia Jia,Kuaifei Xia
Pitaya ( Hylocereus polyrhizus ) is one of the subtropical and tropical fruits, has gained global cultivation interest due to its nutritional, medicinal, and economic value. To improve the adaptability of pitaya on islands or soil salinization, it is of great significance to explore the salt tolerance mechanisms of pitaya. While oxalate has been conventionally characterized as an antinutrient metabolite, emerging evidence underscores its dual role as a stress signaling molecule through homeostatic regulation. Here, we demonstrate that dynamic control of oxalate catabolism is essential for pitaya's salt adaptation. Transcriptomic profiling revealed coordinated upregulation of the oxalate-degrading gene HuAAE 3 under salt stress, accompanied by enhanced oxalate accumulation. Notably, the transcription factor HubHLH36 emerged as a central regulator responding to both ionic stress and exogenous oxalate. Molecular validation through yeast one-hybrid and dual-luciferase assays unveiled confirmed that HubHLH36 directly activates HuAAE3 transcription by binding to its promoter. Transient overexpression of HuAAE3 or HubHLH36 in pitaya plants significantly reduced oxalate levels, enhanced salt/oxalate tolerance, and alleviated reactive oxygen species (ROS) accumulation. Conversely, virus-induced gene silencing (VIGS) of these genes elevated oxalate content, compromised stress tolerance, and exacerbated ROS production. Heterologous expression in Arabidopsis further validated the conserved function of HubHLH36-HuAAE3 module in conferring salt/oxalate tolerance with ROS mitigation. Our findings establish a novel transcriptional regulatory module where HubHLH36 modulates oxalate homeostasis via HuAAE3 activation, thereby mitigating oxidative stress under salt stress. These mechanistic insights provide genetic targets and theoretical frameworks for developing salt-tolerant pitaya cultivars, offering promising solutions for sustainable agriculture in saline environments.
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FOOD RESEARCH INTERNATIONAL
·
2025
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IF 8
Shiyu Wu,Zhenxiao Sun,Jian Guo,Dongmei Li,Xinping Lin,Sufang Zhang,Chaofan Ji
Soy sauce, a traditional fermented soybean condiment originating in Asia, is now enjoyed worldwide. As its popularity grows, ensuring both its safety and high quality has become increasingly important. In industrial production, starter cultures are essential for controlling microbial communities and enhancing the safety and quality of the product. This study initially screened bacterial starter cultures with the ability to degrade biogenic amines (BAs), followed by their mono- or co-inoculation in soy sauce fermentation. The results showed that mono-inoculation with Tetragenococcus halophilus C88, Tetragenococcus halophilus C13, Lactiplantibacillus plantarum H6 and Staphylococcus piscifermentans S1 reduced BAs levels by 28.13 %, 29.55 %, 18.90 % and 14.63 % respectively. Concurrently, these treatments increased the total volatile organic compounds (VOCs) by 28.00 %, 82.68 %, 46.67 % and 80.13 % respectively. Notably, co-inoculation of T. halophilus C88 with Lpb. plantarum H6 led to superior BA degradation decreased by 31.39 %, while co-inoculating T. halophilus C13 and S. piscifermentans S1 resulted in a greater increase in VOCs compared to mono-inoculation, achieving a 105.07 % rise compared to natural fermentation. These findings indicate that synergistic interactions between co-fermentation starters can improve soy sauce quality. Additionally, the research highlights the potential of using complementary starter cultures to influence the metabolic pathways of key VOCs, offering valuable insights for enhancing both the safety and flavor profiles of soy sauce in industrial production.
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危险品化学品经营许可证（带存储）