APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
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2023
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IF 3
Sun Ziyi,Zhang Gaoning,Li Xiaoru,Cui Qingqi,Gong Lili,Tian Zhenhua
This study investigated the chemical and volatile characteristics of sea buckthorn fruits from three different regions in China. The chemical composition of the volatile oil was determined by using a non-targeted gas chromatography and mass spectrometry (GC/MS) method and the differences in chemical composition among the three producing areas were compared by heatmap providing a visual basis for researchers. A total of 93 compounds were identified, including 52 compounds from the Northeast China, 51 from the Xinjiang region, and 37 from Inner Mongolia region. Then, the in vitro antioxidant activity of sea buckthorn fruit oil was measured using DPPH, ABTS, and SOD inhibition tests, and the results showed that sea buckthorn fruit oil in northeast China was the strongest antioxidant, followed by Inner Mongolia and Xinjiang. The results of the CCK-8 experiment indicated that within the tested concentration, there is no cell cytotoxicity of the essential oil in human umbilical vein endothelial cells (HUVECs) cells. The results could supply reference to distinguish sea buckthorn fruit from different production areas and, meanwhile, clarify the activity and safety of sea buckthorn oil.
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PubMed
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Food Bioscience
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2023
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IF 5.2
Kefan Ouyang,Qian Chen,Hexiang Xie,Qin Zhang,Liling Tao,Hua Xiong,Yulan Du,Jiangang Yan,Xinliang Mao,Qiang Zhao
Arthrospira cell residue (ACR) is a byproduct of the spirulina industry that can be valorized into protein hydrolysates. In this study, we used alkaline protease to hydrolyze ACR and evaluated the effects of hydrolysis time on the properties and functionalities of the hydrolysates. We measured the degree of hydrolysis (DH), particle size, fluorescence, UV‒visible absorption, surface hydrophobicity , emulsification , solubility, and antioxidant activity of the hydrolysates. The DH reached a maximum of 10.2% ± 0.6% after 240 min. Hydrolysis also altered the structure and conformation of the ACR protein, as shown by changes in fluorescence, UV-visible absorption, and FTIR spectra. Hydrolysis enhanced the solubility, surface hydrophobicity , and emulsifying activity of the hydrolysates but not the emulsion stability. The hydrolysates also exhibited higher antioxidant activity than ACR. These results indicate that enzymatic hydrolysis can convert ACR into valuable protein hydrolysates.
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ACS Applied Materials & Interfaces
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2023
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IF 9.5
Nitong Bu,Lin Wang,Di Zhang,Huimin Xiao,Xiaoman Liu,Xianrui Chen,Jie Pang,Chen Ma,Ruojun Mu
Biodegradable gelatin (G) food packaging films are in increasing demand as the substitution of petroleum-based preservative materials. However, G packaging films universally suffer from weak hydrophobicity in practical applications. Constructing a hydrophobic micro/nanocoating with low surface energy is an effective countermeasure. However, the poor compatibility with the hydrophilic G substrate often leads to the weak interfacial adhesion and poor durability of the hydrophobic coating. To overcome this obstacle, we used (3-aminopropyl) triethoxysilane (APS) as an interfacial bridging agent to prepare a highly hydrophobic, versatile G nanocomposite film. Specifically, tannic acid (TA)-modified nanohydroxyapatite (n-HA) particles (THA) were introduced in G matrix (G-THA) to improve the mechanical properties. Micro/nanostructure with low surface energy composed of nanozinc oxide (Nano-ZnO)/APS/stearic acid (SA) (NAS) was constructed on the surface of G-THA film (G-THA/NAS) through one-step spray treatment. Consequently, as-prepared G-THA/NAS film presented excellent mechanics (tensile strength: 7.6 MPa, elongation at break: 292.7%), water resistance ability (water contact angle: 150.4°), high UV-shielding (0% transmittance at 200 nm), degradability (100% degradation rate after buried in the natural soil for 15 days), antioxidant (78.8% of 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity), and antimicrobial (inhibition zone against Escherichia coli: 15.0 mm and Staphylococcus aureus: 16.5 mm) properties. It should be emphasized that the bridging function of APS significantly improves the interfacial adhesion ability of the NAS coating with more than 95% remaining area after the cross-cut adhesion test. Meanwhile, the G-THA/NAS film could maintain stable and long-lasting hydrophobic surfaces against UV radiation, high temperature, and abrasion. Based on these multifunctional properties, the G-THA/NAS film was successfully applied as a liquid packaging material. To sum up, we provide a feasible and effective method to prepare high-performance green packaging films.
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BIOMACROMOLECULES
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2023
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IF 6.2
Zhiwen Zeng,Jiayi Guo,Guangxin Shen,Cuiping Guo,Dating Pei,Daohuan Lu,Zhijie Geng,Jun Huang,Shan Yu
Chronic wound infection often leads to irregular tissue closure and accompanies delayed healing and economy issues. Developing an ideal wound dressing that can control the occurrence of antibacterial infections and biological responses is highly desirable. In this study, a multifunctional hybrid hydrogel (GS@EG–Cu–CA NPs) containing synthesized thiolated gelatin, methacrylated silk fibroin, and (−)-epigallocatechin gallate–copper ionic–carrageenan nanoparticles (EG–Cu–CA NPs) was engineered by a thio–ene click reaction. The metal–polyphenol EG–Cu–CA NPs were encapsulated with kappa-carrageenan to enhance its aqueous-soluble, mechanical, and bioactive properties and endowed the hydrogel dressing with fascinating antibacterial, antioxidation, and nitric oxide (NO) generation by catalyzing. The hybrid hydrogels also illustrated a favorable cytocompatibility. Benefiting from the thio–ene click reaction, the hybrid hydrogels were injected and photocured rapidly in situ to cover an irregular wound. In an SD rat full-thickness skin-wound-infected model, the methicillin-resistant Staphylococcus aureus-infected wound covered with GS@EG–Cu–CA NPs was almost completely healed after 10 days. This study presents a facile design of hydrogel dressing incorporating metal–polyphenol nanoparticles, which demonstrates a promising potential way for dealing with effective wound infection management and other complicated wound healings.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2023
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IF 8.6
Yan Li,Mingjie Chen,Qing-Shan Shi,Xiaobao Xie,Yanzhu Guo
Lignin is a phenolic residue from biomass refinery industries. Biomass fractionation techniques keep emerging because of the pursuit of improved revenues from biomass refineries, which generates lignin structurally different from conventional one, i.e. , the kraft lignin. To understand the impact of emerging biomass fractionation processes on the antioxidant properties of lignin, six lignins were isolated from pine wood, and structurally analyzed with ultraviolet/visible spectroscopy, Fourier transform infrared spectroscopy, hetereonuclear single quantum coherence nuclear magnetic resonance, 31 P nuclear magnetic resonance, and gel permeation chromatography. The antioxidant activity of isolated lignins was determined by DPPH assay, ABTS assay, superoxide anion radical scavenging assay, and hydroxyl radical scavenging assay. The structure and antioxidant properties of lignin were discussed accordingly. The results of this study would provide solid data guiding the isolation of lignin-based high-performance antioxidants from biomass.
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COMPOSITES PART B-ENGINEERING
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2023
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IF 13.1
Hui Yan,Yumei Qin,Yanyan Li,Yongqi An,Haoshuang Wu,Chong Chen,Li Yang,Fanjun Zhang,Rifang Luo,Qing Jiang,Yunbing Wang
Coagulation, inflammation, and hyperplasia frequently restrict the effectiveness of cardiovascular stents . It is critical to modify stent surfaces to allow for in situ tissue healing. Interrelated coagulation and inflammation can impede endothelialization and are critical to the long-term performance of cardiovascular implants . This study proposes a relatively bio-inert “Anti-fouling plus” coating that is effective in anti-coagulation and anti-inflammation at the early stage of implantation and provides mild inflammatory responses during tissue remodeling process. The phosphorylcholine (PC)-based polymer PM(PCLA) is reacted onto a polydopamine-treated polylactic acid (PLA) surface. PC-based polymers that mimic cell membranes present remarkable antiinflammation and anticoagulation properties. The composite of epigallocatechin gallate (EGCG) and tempol is loaded on the PM(PCLA) coating to achieve “anti-fouling plus” which can mediate inflammatory responses by scavenging reactive oxygen species. In vitro hemocompatibility and cell tests demonstrate the effectiveness of coatings in inhibiting thrombus and inflammation. Interestingly, this study originally reveals a novel strategy that the anti-fouling plus coating can direct vascular tissue healing on stent via promoted endothelialization and mediated contractile smooth muscle cell phenotype by switching inflammatory cells to a pro-repair phenotype during tissue healing process, even if the coating is relatively bio-inert.
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ACS Food Science & Technology
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2023
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IF 2.3
Hao-Xiang Gao,Nan Chen,Qiang He,Bi Shi,Zhi-Long Yu,Wei-Cai Zeng
This study aimed to construct zeolite/chitosan-based magnetic microparticles (MMPs) using ZSM-5 zeolite, Fe3O4, and chitosan with incorporated Ligustrum robustum (Rxob.) Blume polyphenol extract (LRE) for oil quality control. Magnetic characterization and X-ray diffraction analysis demonstrated that the obtained MMPs had a saturation magnetization of 12.07 emu/g and showed magnetic separation ability due to the presence of ferric oxide crystals. Meanwhile, MMPs exhibited good resistance against heating and had a uniform structure with an intermediate diameter of 125.79 μm. Furthermore, scanning electron microscopy and sustained-release analysis indicated that MMPs could well embed LRE and slowly release LRE in weakly polar solvents. Finally, the oil protection experiments demonstrated that MMPs could inhibit the quality deterioration of peanut oils during the frying process and show good recyclability. The findings provide promise for using magnetic microparticles in oil quality control during high-temperature processing in agriculture and food industries.
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FOOD CHEMISTRY
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2023
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IF 8.8
Wenjuan Wang,Rui Sun,Suping Ji,Qiang Xia
This work investigated the characteristics of pea protein isolate and κ-carrageenan complexes in stabilizing curcumin-loaded emulsions. The complexes structured by electrostatic attraction exhibited biphasic wettability with increased three-phase contact angles close to 90°. Morphological differences in the complexes were the critical factor influencing their emulsifying ability at various pH. As a steric barrier via increasing net negative charge (up to −54.7 ± 2.4 mV) and adsorbed protein content (92.57 %–97.61 %), the interfacial layer could retard droplet coalescence and improve emulsions stability. Rheological tests verified the higher viscoelasticity of emulsions by raising the oil fraction. After 4 weeks of heating treatment, the chemical stability of curcumin was prominently enhanced from 18.6 ± 0.2 % to 64.3 ± 5.7 %. The confirmed synergistic antioxidant activity between grape seed oil and curcumin in emulsions might facilitate the development of specific functional delivery systems in foods.
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ACS Applied Materials & Interfaces
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2023
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IF 9.5
Lili Zhang,Xin Gao,Yuanmiao Feng,Yanhong Yan,Hailin Zhu,Shuiping Liu,Yuan Yu,Bin Yu
The great problem of food spoilage is causing food waste worldwide. However, prolonging the shelf life of food and responding to spoilage are good strategies for dealing with this problem. Herein, we present the design of multifunctional chitosan-based hydrogel-incorporated tryptophan carbon quantum dots (Trp-CDs) with antibacterial properties and pH-mediated fluorescence response (pH = 1–13). This chitosan (CS)/tannic acid (TA)/Trp-CDs hydrogel (CTTC hydrogel) was rapidly formed by a high density of hydrogen bonds and has the advantages of good mechanical properties (1628.55 kPa, 280%), washability (5–10 min), antioxidant activity (95.83%), and antibacterial properties. In practical application with fruits, the hydrogel significantly prolonged the shelf life of strawberries by at least 5 days and oranges by 20 days under ambient conditions. In particular, the hydrogel has good pH-mediated fluorescence responsiveness and reversibility due to doping with Trp-CDs, laying a foundation for its application in response to food spoilage.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
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IF 8.2
Yu Song,Jing Lei,Jie Li,Jin Wang,Ju-Li Hu,Xiao-Qin Zheng,Yi-Chen Hu,Liang Zou,Ding-Tao Wu
Buckwheat green leaves are commonly consumed as functional tea materials due to their various beneficial effects. Although buckwheat green leaves have abundant soluble dietary fibers (SDFs), the information about their structural properties and functional properties remains unknown, largely hindering their applications as functional/health products. Hence, to enhance the usage and application of SDFs from buckwheat green leaves as value-added health products, the structures and biological activities of SDFs derived from different buckwheat green leaves were investigated and compared. Results revealed that SDFs derived from Tartary buckwheat green leaves (TBSDF) and common buckwheat green leaves (CBSDF) were rich in complex pectic-polysaccharides, mainly composing of homogalacturonan (HG) and rhamnogalacturonan I (RG I) pectic domains. Besides, TBSDF had higher proportion of RG I pectic domains than that of CBSDF. Furthermore, the existence of a high content of complex pectic-polysaccharides in TBSDF and CBSDF could contribute to their various biological activities, such as antioxidant, antiglycation, fat/bile acid binding, anticancer, and prebiotic effects. These results can provide some new insights into further development of buckwheat green leaves and related SDFs as value-added health products.
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JOURNAL OF MOLECULAR STRUCTURE
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2023
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IF 3.8
Na Li,Baijian Feng,Yongguang Bi,Fansheng Kong,Zhong Wang,Shaofan Tan
Tea polyphenols (TP) is popular due to strong biological activity, however, the instability of TP limits its application. In this study, inclusion complexes (TP-SBE) of TP with sulfobutyl ether β-cyclodextrin (SBE) were synthesized to improve the stability and bioactivity of TP. To retain more active components and obtain powder with good morphology, TP-SBE was prepared by freeze-drying and characterized by Ultraviolet-visible, Fourier Transform Infrared Spectroscopy, Scanning Electron Microscopy, Thermogravimetric-differential scanning calorimetry and X-ray Diffraction Spectroscopy, which showed that the TP was successfully encapsulated by SBE and the thermal stability was improved. The effects of mass ratio, inclusion temperature, stirring speed, and inclusion time on the encapsulation rate of the synthesized TP-SBE were also determined, and the encapsulation rate reached 98.29% ± 1.65% under the optimal conditions. The in vitro activity assay showed that the IC 50 of TP-SBE on 2,2-Diphenyl-1-picrylhydrazyl and 2,2-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) was 16.24±0.99 μg/mL and 5.13±0.17 μg/mL, and the scavenging effect on free radicals and α-glucosidase was more substantial than that of TP. The release of TP slowed down by SBE encapsulation and showed pH-selective. These data suggested that TP can be developed as a nutraceutical with antioxidant and hypoglycemic properties.
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CHEMICAL ENGINEERING JOURNAL
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2023
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IF 15.1
Lei Zhang,Minglu Hao,Liang Yao,Cheng Xing,Qingmei Wen,Zhining Zhang,Jingmou Yu,Jun Wang,Dongming Xing,Tao Zheng,P. Chen
Anthocyanins, a class of polyphenols possessing potent antioxidant properties, are limited in their applicability in biomedical industries owing to their low stability. This study harnessed the bioactive silk sericin to encapsulate anthocyanins and thereby conferred enhanced stability and multifunctional bioactivity. Meanwhile, experimental and computational methods were used to systematically probe the interaction between silk sericin and anthocyanins. The hydrogel formulation of silk sericin displayed higher encapsulation efficiency and concomitant improvements in stability under diverse conditions such as pH, metals, and temperature. Molecular simulations revealed that the amino acids, phenylalanine and tyrosine, with a hairpin structure were pivotal in the interaction between the two components, culminating in the formation of nanocomposites, a finding corroborated by the experimental results using various spectroscopic techniques. Significantly, the encapsulated anthocyanins exhibited enhanced bioactivity, particularly in their reactive oxygen species (ROS) scavenging ability. Leveraging the potent antioxidant properties of silk sericin and anthocyanins, the formulation evinced ROS-triggered wound healing functions by modulating factors such as TGF-β, IL-1β, TNF-α, IL-10, and VEGF. This study highlights the potential of utilizing two bioactive components derived from the same sericultural system to enhance stability and regulate bioactivity in tissue engineering applications.
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危险品化学品经营许可证（带存储）