Nanomaterials
·
2019
·Vol. 9
·期 8
·1165
·
IF 4.034
Zhezhe Wang,Xuechun Xiao,Yue Yang,Tong Zou,Xinxin Xing,Rongjun Zhao,Zidong Wang,Yude Wang
A new high performance fluorescence assay for detection of Ag+based on CdS quantum dots (QDs) using L-Aspartic acid (L-Asp) as a stabilizer was proposed in this work. The CdS quantum dots conjugation with L-Aspartic acid (L-Asp@CdS QDs) were successfully synthesized via a simple hydrothermal process. The QDs have a fluorescence emission band maximum at 595 nm with a quantum yield of 11%. The obtained CdS QDs exhibit a particle size of 1.63 ± 0.28 nm and look like quantum dot flowers. Basically, the fluorescence intensity of L-Asp@CdS QDs can be enhanced only upon addition of Ag+and a redshift in the fluorescence spectrum was observed. Under optimum conditions, the fluorescence enhancement of L-Asp@CdS QDs appeared to exhibit a good linear relationship in between 100–7000 nM (R2= 0.9945) with the Ag+concentration, with a detection limit of 39 nM. The results indicated that the L-Asp@CdS QDs were well used in detection for Ag+as fluorescence probe in aqueous solution with high sensitivity and selectivity. Moreover, the sensing system has been applied in detection Ag+in real water samples. The recovery test results were 98.6%~113%, and relative standard deviation (n = 5) is less than 3.6%, which was satisfactory.Keywords:L-Aspartic acid;CdS quantum dots;fluorescence assay;Ag+detection;Fluorescence enhancement
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高影响因子
Journal of Materials Chemistry B
·
2019
·Vol. 7
·期 44
·7052-7064
·
IF 5.047
Hui Chi,Anlong Jiang,Xiaoyan Wang,Guanghua Chen,Chengchao Song,Ravi Kumar Prajapati,Ang Li,Zecheng Li,Jiaxin Li,Zhengye Zhang,Ye Ji,Jinglong Yan
Micro–nano based fibrous scaffolds have been extensively studied in regenerative medicine. Bone marrow stem cells (BMSCs) and BMP2-derived peptides, two other important components for tissue engineering, have been successfully used for bone regeneration. However, a scaffold that specifically captures BMSCs and delivers BMP2-derived peptides to promote osteogenic differentiation of enriched BMSCs has not been reported. In this study, a microfiber scaffold was constructed by coaxial electrospinning technology using a polyvinylpyrrolidone/bovine serum albumin/BMP2-derived peptide compound as the core solution and a polycaprolactone/collagen I compound as the shell solution. The scaffolds were further functionalized by covalent grafting of a BMSC affinity peptide (E7) to develop a dual drug release system for the delivery of the BMP2-derived peptide and E7. Structural analysis indicated that the microfibers had a uniform diameter and homogeneous core–shell structure. Fourier transform infrared spectroscopy (FTIR) revealed that E7 was covalently bonded onto the surface of the fibers. In vitro, the E7-modified scaffolds promoted the initial adhesion of BMSCs and were more favorable for BMSC survival. Furthermore, the BMP2-derived peptide loaded in the E7-modified scaffolds was released in a sustained manner and retained bioactivity, significantly improving the osteogenic differentiation of BMSCs. In vivo, scaffolds loaded with the BMP2-derived peptide and E7 (PCME scaffolds) led to enhanced new bone formation and defect closure in a rat calvarial defect model. Overall, the PCME scaffold simultaneously facilitated all three of the essential elements needed for bone tissue engineering, providing a promising method for bone regeneration.
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JOURNAL OF COORDINATION CHEMISTRY
·
2018
·
IF 1.703
Qian Li,Qiong Wei,Pei Xie,Li Liu,Xin-Xin Zhong,Fa-Bao Li,Nian-Yong Zhu,Wai-Yeung Wong,Wesley Ting-Kwok Chan,Hai-Mei Qin,Njud S. Alharbi
A series of three-coordinate dinuclear copper halide complexes containing acarbazolyl monodentate phosphine ligand, [CuX(dppc)]2 (dppc =3,6-di-tert-butyl-9-[2-(diphenylphosphino)phenyl]carbazole, X = I (1), Br (2), and Cl (3)), were synthesized, and their molecular structures and photophysical properties were investigated. The structural analysis reveals that two copper(I) centers are bridged by two halogen ligands to form a dinuclear structure with a four-membered Cu2X2 ring. Crystal structures of 1-3 contain 1-D supramolecular arrays constructed by intermolecular C–H···π interactions. These complexes exhibit blue to green emission in the solid state at room temperature and have peak emission wavelengths at 492–495 nm with microsecond lifetimes and low emission quantum yields (< 0.01%). The emission of 1-3 mainly originates from MLCT, XLCT, and IL (intraligand) transitions. The three complexes displayed good thermal stability.
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FOOD HYDROCOLLOIDS
·
2018
·
IF 5.089
Junhui Li,Shan Li,Yangfan Zheng,Hua Zhang,Jianle Chen,Lufeng Yan,Tian Ding,Robert J. Linhardt,Caroline Orfila,Donghong Liu,Xingqian Ye,Shiguo Chen
The recovery of pectic polysaccharides with high rhamnogalacturonan I (RG-I) branches from citrus canning processing water was achieved in a previous study aimed at reducing chemical oxygen demand and benefiting both process economics and the environment. However, the large molecular size and poor in vivo bioavailability of these polysaccharides limit the application of these pectic polysaccharides in functional foods. We report the development of an ultrafast and green approach to depolymerize pectic polysaccharides using an ultrasound-accelerated metal-free Fenton chemistry , relying on H 2 O 2 /ascorbic acid. The results show that ultrasound enhances the efficiency of H 2 O 2 /ascorbic acid system to degrade pectin into 7.9 kDa pectic fragments within 30 min through both chemical effects (increasing the amount of hydroxyl radicals and lowering activation energy of H 2 O 2 decomposition) and mechanical effects (disaggregating polysaccharide clusters). The backbones of the resulting fragments mainly correspond to RG-I patterns (molar ratio galacturonic acid (GalA): rhamnose (Rha) ∼ 1.06:1) with a high degree of rhamnose branching. Free radicals preferentially act on the GalA backbone in the HG region and maintain the RG-I region. Antitumor activities, assessed using human breast cancer cells (MCF-7), suggest that the resulting fragments significantly inhibit cancer cell growth and that activity increases with decreasing molecular weight. The resulting ultralow molecular weight pectic fragments have potential application for the development of functional foods and antitumor drugs.
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Journal of Biophotonics
·
2018
·Vol. 12
·期 3
·e201800237
·
IF 3.768
Yubin Liu,Duyang Gao,Mengze Xu,Zhen Yuan
In this study, CuS nanoparticles with optical absorption covering both near-infrared I (NIR-I) and NIR-II biological windows were prepared and served as the contrast agents for multispectral photoacoustic imaging. The physiological parameters including concentrations of deoxyhemoglobin and oxyhemoglobin as well as the water content in the tumor location were quantified based on the multispectral photoacoustic reconstruction method. More importantly, the concentration of CuS nanoparticles/drugs accumulated in the tumor was also recovered after intravenously injection, which are essential for image-guided cancer theranostics. In addition, phantom and in vivo experimental tests were performed to inspect and compare the imaging depth and signal-to-noise ratio (SNR) between the two NIR biological windows. Interestingly, we discovered that a higher SNR was obtained in the NIR-II window than that in the NIR-I window. Meanwhile, the multispectral imaging results also demonstrated that the imaging contrast and penetration depth in the NIR-II window were also significantly improved as compared to those from the NIR-I window.
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高影响因子
MOLECULES
·
2018
·Vol. 23
·期 5
·1102
·
IF 3.098
Yanxue Cai,Keren Kang,Qianru Li,Yu Wang,Xiaowei He
A reliable lateral flow immunoassay (LFIA) based on a facile one-step synthesis of single microspheres in combining with immunochromatography technique was developed to establish a new point-of-care test (POCT) for the rapid and early detection of cardiac troponin I (cTnI), a kind of cardiac specific biomarker for acute myocardial infarction (AMI). The double layered microspheres with clear core-shell structures were produced using soap-free emulsion polymerization method with inexpensive compounds (styrene and acrylic acid). The synthetic process was simple, rapid and easy to control due to one-step synthesis without any complicated procedures. The microspheres are nanostructure with high surface area, which have numerous carboxyl groups on the out layer, resulting in high-efficiency coupling between the carrier and antibody via amide bond. Meanwhile, the red fluorescent dye, Nile-red (NR), was wrapped inside the microspheres to improve its stability, as well to reduce the background noise, because of its higher emission wavelength than interference from real plasma samples. The core-shell structures provided different functional areas to separate antibody and dyes, so the immunoassay has highly sensitive, wide working curves in the range of 0–40 ng/mL, low limits of detection (LOD) at 0.016 ng/mL, and limits of quantification (LOQ) at 0.087 ng/mL with coefficient of variations (CV) of 10%. This strategy suggested an outstanding platform for LFIA, with good reproducibility and stability to straightforwardly analyze the plasma samples without washing steps, thereby reducing the operating procedures for non-professionals and promoting detection efficiency. The whole detection process can be completed in less than 15 min. This novel immunoassay offers a reliable and favorable analytical result by detecting the real samples, indicating that it holds great potential as a new alternative for biomolecule detection in complex samples, for the early detection of cardiac specific biomarkers.Keywords:cardiac troponin I;core-shell microspheres;lateral flow immunoassay;point-of-care tests;clinical application
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2015
·
IF 2.353
Chenchen Zhang,Shan Jiang,Kun Li,Mei Wang,Rongrong Zhu,Xiaoyu Sun,Qingxiu Wang,ShiLong Wang
The excited triplet state of tanshinone I (Tan I) extracted from the traditional Chinese medicine Salvia miltiorrhiza Bunge was characterized by laser flash photolysis. The synergic effect of Tan I on the phototherapy of cancer cells with curcumin (Cur) was also investigated by MTT assay because the excited energy transfer from the triplet state of Tan I ( 3 Tan I ∗ ) to Cur occurred. At the same time, the characteristic absorption spectra of 3 Tan I ∗ were recorded, and its molar absorption coefficient and rate constants for several excited energy transfers were obtained. The photo-therapeutic effect of Cur is enhanced by combination with Tan I.
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INORGANIC CHEMISTRY
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2013
·
IF 4.593
Yu-Peng Zhou,En-Bao Liu,Jin Wang,Hsiu-Yi Chao
Newly prepared gold(I) acetylide-based luminescent cation sensors 1c and 1d, which bear a novel three–armed flexible conformation, together with control molecule 2c, were synthesized in four steps. 1c and 1d exhibit the π → π* absorption of the acetylide ligands in fluid solutions and produce the 3(ππ*) emission of the acetylide ligands in the solid state and degassed THF solution. The 3(ππ*) emission of 1c in DMSO can be turned on upon addition of Ag+. The binding ratio between 1c and Ag+ as well as the binding constant log K were determined as 1:1 and 4.35 ± 0.12 respectively by UV–vis titration experiments. The formation of [1c·Ag]+ adduct, which leads to the appearance of new up-field peaks, was confirmed by 1H NMR spectroscopic titrations. The control 1H NMR titration experiments for the acetylide-free analogue 1a and single-armed analogue 2c indicate the acetylide groups and tripodal structures are responsible for the binding of Ag+. The control experiment for tripodal gold(I) acetylide analogue 1d suggests the change of PPh3 with P(2-Py)Ph2 induces similar binding affinity toward Ag+ but less selectivity toward Ag+. Free 1c also exhibits the anion binding affinity toward F–, but the Ag+ adduct [1c·Ag]+ shows less affinity toward F–.
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高影响因子
ELECTROANALYSIS
·
2016
·Vol. 28
·期 9
·2044-2049
·
IF 2.471
Juanjuan Zuo,Xiaoyu Zhao,Xiaocui Ju,Shue Qiu,Wenshuai Hu,Ting Fan,Juankun Zhang
A novel molecularly imprinted polymer (MIP) sensor for rapid determination of cardiac troponin (cTnI) was established. Since it can bind to the template molecule cTnI specifically, it can be used to detect concentration of cTnI in serum without much sample pretreatment. What's more, the electrochemical signals depend on the concentration of template molecules. The synthetic sensor possesses advantages including simplicity, high specificity, low cost of preparation, good chemical and mechanical properties, sensitive and label-free determination. The synthetic sensor shows good dynamic linearity at concentration range from 0.05 to 5.00 nM. The limit of detection (LOD) was found to be 0.027 nM. The detection time of whole process was within 5 minutes.
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JOURNAL OF PHARMACY AND PHARMACOLOGY
·
2016
·Vol. 68
·期 6
·751-761
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IF 2.363
Rongbin Huang,Lei Xiang,Ying Liu,Xiangping Deng,Xuan Cao,Lanfang Li,Cuiyun Yu,Yanming Chen,Guotao Tang
ObjectivesTo develop an acid trigger release of antitumour drug delivery carriers, pH-sensitive amphiphilic poly (ethyleneglycol)-imine-benzoic-dipalmitate (PEG-I-dC16) polymers were designed and synthesized and the drug-loaded micelles were evaluated in vitro.MethodsPEG-I-dC16 synthesized by Schiff base synthetic method and characterized by 1H-NMR. To determine the drug-loading capacity, doxorubicin (DOX) was encapsulated in the micelles using membrane dialysis method. Zeta potential, particle size, drug-loading capacity, in vitro drug release in different pH conditions and cytotoxicity evaluation of micelles were carried out comparing with non-acid liable PEG–amide–benzoic–dipalmitate (PEG-A-dC16) polymers micelles. The cellular uptake and intracellular distribution of DOX were detected by flow cytometry and confocal laser scanning microscope.Key findingsDrug-loading capacity and encapsulation efficiency of micelle (PEG molecular weight 2k) were 12.7 ± 1.1% and 49.8 ± 2.2%, respectively. The average particle size was 72.3 ± 2.5 nm. The DOX release rate of PEG-I-dC16 micelles is much higher at pH 6.5 than at pH 7.4. DOX cellular uptake and nuclear accumulation of PEG-I-dC16 micelles were more efficiency than that of PEG-A-dC16 micelles.ConclusionThe pH-sensitive PEG-I-dC16 micelles could be a promising drug delivery system for anticancer drugs.
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LANGMUIR
·
2017
·
IF 3.833
Yumin Leng,Kai Jiang,Wentai Zhang,Yuhui Wang
Metal-mediated interlocking rings won the 2016 Nobel Prize in Chemistry. The metal-directed interlocking rings in macromolecular systems (e.g., proteins) may be similar to the form of Maxwell’s electromagnetic waves; the metal ions may shuttle among the rings in the special environment. To verify this hypothesis, we designed a general approach to synthesize the multicolored gold nanoparticles (GNPs) mediated by Au(I)-directed interlocking rings in proteins. The Au(I) ions shuttled among these interlocking rings in the strong alkaline solution. Through the rapid nucleation method, the multicolored GNPs of different morphology and sizes were synthesized in the multiple honeycombed templates. On the basis of the “three-color” principle of Thomas Young, we extracted the red, green, and blue (RGB) alterations of GNPs to fabricate a visual sensor array for protein discrimination. The fingerprints (ΔRGB) were obtained from the target proteins and fed into computer programs. The proposed sensing platform was also applied to detect lysozyme in human tears with satisfactory results. Importantly, we forecasted that lysozyme could be the effective drug for curing dacryocystitis and nasolacrimal duct obstruction diseases.
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JOURNAL OF POLYMER RESEARCH
·
2017
·Vol. 24
·期 4
·1-7
·
IF 1.615
Li Hu,Zhou Zhen,Liu Jingcheng,Zheng Xiangfei,Xu Wenjia,Ji Changwei,Shi Weidong,Liu Ren,Liu Xiaoya
Hexakis(4-formylphenoxy) cyclotriphosphazene (HAPCP), substitution reaction product of hexachlorotriphosphazene (HCTP) with p-hydroxybenzaldehyde, was reduced by sodium borohydride to afford hexakis[4-(hydroxymethyl) phenoxyl] cyclotriphosphazene (HHPCP). The hydroxyl group of this compound was then protected by the reaction with di-tert-butyl dicarbonate ester (DBDC) in the presence of 4-dimethylaminopyridine (DMAP) as catalyst to give t-butyloxy carbonyl (tBOC) protected HHPCP. Fourier-transform infrared spectroscopy (FT-IR) and proton nuclear magnetic resonance ( 1 H NMR) were employed to characterize the chemical structure, indicating that the synthesis was successful. Thermal behavior was characterized by thermogravimetric analysis (TGA), the results showed that the initial thermal decomposition temperature occurred at approximately 170 °C, X-ray diffraction analysis (XRD) results showed that the tBOC protected HHPCP derivatives had amorphous form, and performed excellent film-forming property. Upon irradiation to 365 nm exposure, the photoacid generated proton H + , which can catalyze the deprotection of acid labile tBOC group. This type of novel molecular glass photoresist based on hexachlorotriphosphazene showed high sensitivity, making it possible to fabricate 1 μm line and space pattern under i-line exposure.
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