Nature Communications
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2026
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IF 18.1
Jianping Xu,Jian Zhang,Xiaotong Wang,Tengfei Zhao,Qingsheng Qi,Qian Wang
Lignin-related aromatics are promising renewable feedstocks, but their microbial conversion into useful compounds is fundamentally constrained by substrate toxicity and metabolic flux imbalance. Here, we report an autonomous microbial system in Corynebacterium glutamicum , which integrates evolutionary engineering with multiplexed dynamic control to overcome these constraints. This system features a robust, evolved chassis with reinforced cellular defenses and upgraded aromatic efflux machinery. We engineer a layered, multi-input regulatory network that enables real-time, substrate-responsive and precise control of metabolic pathways. This system achieves 99.89% conversion of diverse lignin-related aromatics to protocatechuic acid and subsequently achieves a titer of 53.40 g L⁻¹. This further supports high-titer production of value-added downstream chemicals, including cis,cis -muconic acid and β-ketoadipic acid with titers of 51.90 g L⁻¹ and 38.33 g L⁻¹, respectively. This work establishes a versatile and scalable paradigm for the autonomous bioprocessing of lignin feedstocks into sustainable value-added products. Biological valorization of lignin is hindered by substrate toxicity and metabolism inefficiency. Here, the authors established a platform for lignin-derived aromatics tolerance engineering and multi-node dynamic pathway control in Corynebacterium glutamicum .
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2025
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IF 3.8
Lu Meijun,Zhao Chen,Ma Mo,Shi Hui,Lu Mingming,Tian Yuan,Song Daqian,Zhang Ziwei
The abnormally high expression of glutathione (GSH) in hepatocellular carcinoma (HCC) cells significantly contributes to chemotherapy resistance by neutralizing reactive oxygen species (ROS). To address this problem, we developed a nanocomposite probe (micelle@PDA@MnO 2 NPs) which was composed of free radical-labeled micelles as the core, polydopamine (PDA) as the inner shell and MnO 2 nanosheets as the outer shell. In the HCC microenvironment, the over-expressed GSH triggered the decomposition of the MnO 2 shell, releasing Mn 2+ ions, and subsequently, the acidic condition and over-expressed carboxylesterase 2 (CES2) worked together to mediate the controlled release of nitroxide free radicals (i.e., TEMPONE) from the micelle core. With the help of dual-signal electron spin resonance (ESR) detection, it was found that much more free radicals and Mn 2+ were released in hepatoma cells than in normal liver cells, rendering the proposed nanotherapeutic platform specific to HCC and a good candidate for free radical therapy and chemodynamic therapy (CDT). Moreover, the therapy featured an intelligent self-amplified property: the abundant GSH in the tumor microenvironment rapidly activated the decomposition of the nanocomposite and triggered the release of free radical TEMPONE and Mn 2+ , and TEMPONE and Mn 2+ further amplified the oxidative stress to induce tumor apoptosis via ROS generation and GSH depletion. Finally, in combination with three therapy modes (Mn 2+ -mediated CDT, the oxidative stress enhancement effect of TEMPONE, and PDA-based photothermal therapy), significant therapeutic effects including facilitation of HepG2 cell apoptosis and inhibition of tumor growth in the HCC mouse model were achieved.
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EMBO JOURNAL
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2025
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IF 8.3
KongDu,LiXiaoqin,ZhaoSihua,WangChenliang,CaiZixin,SongSha,GuoYifan,KuangXiaoyu,WangXianping,LiuWenhan,LiuPeng,GuoXiaowei,XuWenyan,WangYirong,ZhaoBin,JinBin,HeLi,MaXianjue
Interorgan communication is vital for tissue homeostasis and health in multicellular organisms, and its disruption can lead to diseases such as cancer. Adipose tissue acts as a key endocrine center, secreting cytokines that influence remote organs. Despite clear links between obesity and increased cancer risk, the underlying mechanisms are unclear. Here, utilizing a Drosophila genetic model combining Gal4-UAS and QF-QUAS tissue-specific transgene expression systems, we reveal that adipose-secreted Spz5 ligand promotes distal epithelial tumor overgrowth and invasion. Mechanistically, Spz5 binds to tumor cell Toll-6 receptors, triggering the degradation of the endocytic adaptor protein AP-2α via Mib1-mediated ubiquitination. Consequently, impaired endocytosis leads to Smoothened (Smo) accumulation on the cell membrane and subsequent activation of the Hedgehog (Hh) pathway. This abnormal Hh activation synergizes with the oncogenic Yorkie (Yki) to drive tumor growth and invasion. Furthermore, tumor-derived Unpaired ligands (Upds) activate the JAK-STAT pathway in the fat bodies, which leads to Hippo pathway-dependent upregulation of spz5 expression. Thus, our study provides insights into the complex regulatory mechanisms by which systemic interorgan communication influences tumor progression.
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Nature Communications
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2025
·Vol. 16
·期 1
·1-11
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IF 15.7
Wang Haibing,Ge Yansong,Shao Wenlong,Xiong Xingyu,Li Guang,Li Nengxu,Hu Xuzhi,Chen Guoyi,Dong Kailian,Ai Wei,Yu Zixi,Zheng Zhimiao,Wang Chen,Yao Fang,Cao Xiaojuan,Li Songzhan,Zhao Jianwei,Ke Weijun,Tao Chen,Hou Yi,Petrozza Annamaria,Fang Guojia
Transparent conductive oxides (TCOs)—foundation of perovskite solar cells (PSCs)—have long been assumed to be stable, and thus their impact on device longevity is frequently overlooked. Herein, we unveil that fluorine doped tin oxide (FTO) suffers from instability under operational stress, exacerbating PSC stability issue. To address this issue, we propose a universal interface engineering strategy employing a scalable thermal evaporation followed by natural oxidation to form an atomically bonded yttrium oxide (Y 2 O 3 ) to strengthen structural stability of FTO. Evaporated yttrium effectively anchors a portion of lattice oxygen within FTO, preventing elemental dissociation. Moreover, the formed Y 2 O 3 featured conformal deposition on rough FTO increases the interfacial adhesion energy, establishing a robust barrier against ion diffusion and carrier nonradiative recombination loss. This approach fortifies the structural integrity of the PSC, leading to dramatically improved operational stability. Unencapsulated devices exhibit negligible performance loss after 1,200 h of continuous illumination. Notably, we achieve power conversion efficiencies of 26.48% (certified at 26.12%) in regular (n–i–p) architectures, 26.34% in inverted (p–i–n) configurations, and 28.47% in tandem structures–among one of the highest reported in their respective categories–underscoring its strong generality and potential for commercialization.
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JOURNAL OF NANOBIOTECHNOLOGY
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2025
·Vol. 23
·期 1
·1-16
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IF 12.6
Yang Deqi,Zhang Ya,Yuan Yun,Peng Beibei,Wang Bingfang,Ren Qinjuan,Chang Zhiqiang,Liu Shuai,Fang Yin
Remote modulation of specific neurons is critical for neural circuit dissection and neurological disease therapy. Traditional neuromodulation strategies applying electrodes and external stimuli may suffer from invasive injuries and insufficient spatiotemporal resolution, respectively. Herein, transcranial optical neuromodulation with precise spatiotemporal control was realized by establishing a nanophotonic neural interface, where the polyethylene glycol-modified two-dimensional Ti 3 C 2 T x (MXene) served as near-infrared (NIR) photoactive nanosheets (PANS). Polymer modification of the nanosheets contributed to a robust PANS-neuron interface, which allowed efficient modulation of non-transgenic neurons with subcellular resolution and millisecond precision. The PANS-enabled transcranial optical neuromodulation not only evoked neuronal action potentials in brain slices with a high synchronization rate of 98.33%, but also facilitated neuronal firing in vivo. With PANS as stimulus targets, the transcranial NIR light can excite neurons in desired brain regions and activate defined neural circuits, thus regulating mouse behaviors with minimal invasiveness. Such nanosheet-enabled transcranial and precise optical neuromodulation, eliminating invasive implants and genetic manipulation, may open opportunities for neurophotonics and neuroscience research. Transcranial optical neuromodulation with precise spatiotemporal control was realized via a nano-enabled optical neural interface, where the polyethylene glycol-modified two-dimensional Ti 3 C 2 T x serves as near-infrared photoactive nanosheets. Such efficient neuromodulation of non-transgenic animals with subcellular resolution and millisecond precision, eliminating invasive implants and genetic manipulation, holds potential for neuroscience research. Graphical abstract
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Science China-Chemistry
·
2025
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IF 9.7
He Yueyue,Wu Shufang,Li Xiaofei,Zhang Xian-Ming,Fu Dongying
Non-covalent interactions play an indispensable role in the construction of molecular ferroelectrics, as they can regulate the relative orientation and stacking mode between molecules. However, the difficulty and challenge lie in precisely designing and regulating the weak intermolecular interactions, ultimately generating macroscopically reversible spontaneous polarization through synergistic effects, thereby achieving ferroelectricity. Herein, we introduced halogenated ammonium Cl-PA + (Cl-PA + is 3-chloropropylaminium) to regulate non-covalent interactions in the structure of hybrid perovskite, and constructed a photoferroelectric semiconductor (Cl-PA) 2 PbCl 4 with large piezoelectric coefficient ( d 33 = 27.4 pC/N) and high Curie temperature ( T c = 353 K) through the synergistic effect of hydrogen bonding and halogen-halogen interactions. Compared with non-ferroelectric (PA) 2 PbCl 4 (PA + is n -propylaminium), the severe distortion of PbCl 6 octahedra, weakened N–H···Cl hydrogen bond between organic cations and inorganic frameworks, enhanced C–H···Cl hydrogen bond between cations, and the additional Cl···Cl interaction in the structure synergistically induce the ferroelectricity of (Cl-PA) 2 PbCl 4 . In addition, the large dipole moment of Cl-PA is also beneficial for constructing molecular ferroelectrics, and the synergetic effect of non-covalent interactions in (Cl-PA) 2 PbCl 4 provides the possibility for dipole flipping and symmetry breaking. Therefore, constructing molecular ferroelectrics through the synergistic effect of non-covalent interactions essentially utilizes dynamic coupling and precise equilibrium of multiple weak interactions to achieve the formation, stability, and external field flipping of spontaneous polarization. Through this collaborative design, ferroelectrics with high spontaneous polarization ( P s ), low coercive field, and wide temperature range can be achieved, which opens up new directions for flexible electronics and energy storage.
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NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
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2025
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IF 3.1
Ge Xinyu,Wu Bozhao,Dou Xiaohui,Sun Mingchen,Wu Ji-ao,Sun Daqing
Background Slow transit constipation (STC) is a prevalent functional gastrointestinal disorder. Gastrodin (GAS), a natural compound, has demonstrated protective effects in intestinal contexts; however, its therapeutic potential and mechanisms of action in STC remain largely unexplored. Methods A total of 30 male C57BL/6 mice (6 weeks old) were used in this study. An STC model was established using loperamide. The therapeutic effects of GAS were systematically evaluated by measuring several parameters: stool output (fecal pellet count and moisture content), intestinal transit rate, and colon histopathology. Furthermore, we analyzed serum and colonic levels of inflammatory cytokines, key neurotransmitters, and aquaporins (AQPs). The integrity of interstitial cells of Cajal (ICC) in the colon was assessed via immunohistochemistry. Finally, the expression levels of proteins associated with the mitogen-activated protein kinase (MAPK) signaling pathway were examined. Results GAS administration significantly improved STC symptoms by promoting defecation, enhancing gastrointestinal motility, and ameliorating histopathological damage in the colon. It also markedly attenuated intestinal inflammation, normalized the expression of neurotransmitters and AQPs, and restored the loperamide-induced depletion of ICC networks. Mechanistically, the protective effects of GAS were associated with the suppression of the MAPK signaling pathway, as evidenced by reduced phosphorylation of its key components: extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38. Conclusion Our findings indicate that GAS possesses significant therapeutic potential for STC by mediating its beneficial effects through the regulation of intestinal inflammation, motility, and water transport, ultimately via the inhibition of the MAPK signaling pathway.
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JOURNAL OF MATERIALS SCIENCE
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2025
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IF 3.9
Meng Kejiangnan,Wu Hongwu,Zhang Chenxing,Han Lei
A facile two-step modification method using dodecyltrimethoxysilane (DTMS) was developed to fabricate a superhydrophobic wood sponge for oil–water separation from natural green renewable balsa wood. First, alkali treatment was performed on balsa wood to remove hemicellulose and lignin to obtain a rough surface and micron-sized pores, preparing flexible balsa wood (FBW). Second, DTMS was grafted onto the surface of FBW via solution impregnation to construct a micro/nano-coating, yielding a dodecyltrimethoxysilane decorated wood sponge (DWS). The DWS surface exhibited a high water contact angle (WCA) of 150.5°, confirming its superhydrophobicity. DWS demonstrated effective oil absorption from oil–water mixtures. Scanning electron microscopy revealed that FBW possessed an interconnected three-dimensional porous network, enabling DWS to achieve high absorption capacities of 12–31 g g −1 for various oils and organic reagents. After 10 cycles, the absorption capacity remained stable, and the WCA was maintained at 143.2°, indicating excellent recyclability. This two-step modification method requires only 5 h of alkali treatment and 2 h of DTMS grafting, and balsa wood is a natural renewable resource. This study provides a potential strategy for developing superhydrophobic materials to address crude oil spills and sewage pollution.
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Nature Communications
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2025
·Vol. 16
·期 1
·1-15
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IF 15.7
She Zhentao,Fu Yiming,He Yingzhu,Yan Gewei,Wu Wanjie,Qin Zhongya,Qu Jianan
High-resolution imaging under physiological conditions is essential for studying biological mechanisms and disease processes. However, achieving this goal remains challenging due to optical aberrations and scattering from heterogeneous tissue structures, compounded by motion artifacts from awake animals. In this study, we developed a rapid and accurate adaptive optics system called multiplexing digital focus sensing anrobod shaping (MD-FSS) for deep-tissue multiphoton microscopy. Under two-photon excitation, MD-FSS precisely measures the aberrated point spread function in approximately 0.1 s per measurement, effectively compensating for both aberrations and scattering to achieve subcellular resolution in deep tissue. Using MD-FSS integrated with two-photon microscopy, we achieved high-resolution brain imaging through thinned or optically cleared skull windows, two near noninvasive methods to access mouse brain, reaching depths up to 600 μm below the pia in awake behaving mice. Our findings revealed significant differences in microglial functional states and microvascular circulation dynamics between awake and anesthetized conditions, highlighting the importance of studying brain function in awake mice through noninvasive methods. We captured functional imaging of fine neuronal structures at subcellular level in both somatosensory and visual cortices. Additionally, we demonstrated high-resolution imaging of microvascular structures and neurovascular coupling across multiple cortical regions and depths in the awake brain. Our work shows that MD-FSS accurately corrects tissue-induced aberrations and scattering through rapid PSF measurements, enabling near-noninvasive, high-resolution imaging in awake, behaving mice.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
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2025
·Vol. 36
·期 29
·1-12
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IF 2.8
Nurbolat Kuanysh,Wu Zhengwei
A nitrogen plasma-assisted surface engineering strategy was developed to functionalize carbon cloth (CC) and enhance the growth and distribution of Bi₂O₃ nanoparticles for supercapacitor applications. The plasma treatment introduced abundant nitrogen-containing functional groups and defect sites on the CC surface, which promoted uniform nucleation during the subsequent precipitation–calcination process. The resulting Bi₂O₃@NCC composite exhibited a high areal capacitance of 2788.7 mF cm⁻ 2 at 1 mA cm⁻ 2 and retained 64.2% of this value at 20 mA cm⁻ 2 . The electrode also demonstrated good cycling stability, with 80.1% capacitance retention after 5000 charge–discharge cycles. Electrochemical impedance spectroscopy (EIS) confirmed reduced charge transfer resistance and enhanced ion diffusion kinetics. This work demonstrates a practical and scalable plasma-assisted approach for improving both the performance and durability of carbon-based composite electrodes for energy storage applications.
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Nature Communications
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2025
·Vol. 16
·期 1
·1-11
·
IF 15.7
Jian Yaping,Zhang Jiacheng,Cao Xuewen,Peng Qin,Zhang Jun,Tian Xuefeng,Du Jianfei,Li Yan,Yuan Yihui,Wang Ning
Efficient recovery of uranium from spent nuclear fuel (SNF) reprocessing in ultra-acidic and radiation-intensive environments is critical for sustainable uranium resources management. However, traditional extractants exhibit instability and reduced uranium adsorption capacity under such harsh conditions. Here, we present a metal-free pyrophosphate-incorporated g-C₃N₄ polymer (PCN x ) featuring unsaturated nitrogen sites and pyrophosphate groups for robust uranyl ion coordination. The inherent stability of g-C 3 N 4 endows PCN x with high resistance to concentrated 12 M HNO 3 and 500 kGy radiation. The optimal PCN 1/3 achieves an uranium adsorption capacity of 75.3 mg g −1 in 12 M HNO 3 and a high removal efficiency of 79.1% in a mixed ion solution, with a high distribution coefficient ( K d ) of 18,964 mL g −1 . This study demonstrates a promising proof-of-concept for designing functional polymers that efficiently recovers uranium from ultra-acidic and radiation-intensive conditions during SNF reprocessing.
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Cell Communication and Signaling
·
2025
·Vol. 23
·期 1
·1-21
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IF 8.9
Jia Yifan,He Muchen,Wang Fan,Zhan Yaqing,Deng Qiwen,Shen Jiantong,Wang Xiwen,Ran Qi,Huang Wenqi,Ling Yihong,Wen Shihong
Background & Aims Dysfunction of the intestinal epithelial barrier (IEB) and gut vascular barrier (GVB) contributes to the development of intestinal ischemia/reperfusion (IR) injury. Tryptophan (TRP), an essential amino acid, plays a crucial role in maintaining intestinal homeostasis, yet its regulatory effects on the GVB following IR remain unexplored. We aimed to better define the role of TRP in intestinal IR in vivo and in vitro. Methods Mice underwent intestinal ischemia/reperfusion (IR) and were fed control, TRP-recommended (TRP-r), or TRP-sufficient (TRP-s) diets. Fecal metagenomic sequencing analyzed microbial composition, and targeted metabolomics quantified tryptophan and its metabolites in intestinal and serum samples. ILA’s effects on barrier integrity were assessed via tight junction protein expression and FITC-dextran permeability assays. RNA sequencing of intestinal endothelial cells elucidated mechanisms by which ILA modulated GVB function. The STAT3-claudin 2 relationship was validated in vitro by ChIP-qPCR. Results TRP supplementation significantly reshaped the gut microbiota, mitigated tissue damage and enhanced the integrity of both the IEB and GVB. Indole-3-lactic acid (ILA), a key tryptophan metabolite, was identified as an important factor in preserving GVB function. Mechanistically, our results show that the aryl hydrocarbon receptor (AhR)/Nrf2/signal transducer and activator of transcription 3 (STAT3) pathway is essential for ILA-mediated improvement of GVB integrity and downregulation of the pore-forming protein claudin 2. Conclusions Our findings highlight the dual role of ILA in reinforcing both IEB and GVB functions and shed light on the molecular mechanisms underlying ILA’s GVB-protective effects. This study implicates that ILA or other AhR-activating metabolites may serve as promising pharmacological agents for alleviating IR-induced intestinal damage. Graphical
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