查找使用阿拉丁产品的科研文献

Mechanically robust and highly hydrated curcumin-loaded lysine-crosslinked carboxymethyl cellulose hydrogels for steroid-free atopic dermatitis treatment

Zhang Xinyan,Zhou Jingqiu,Han Xue,Xing Xu,Yang Kun,Wu Tao,Li Hao,Hao Xujia,Du Qiang,Liu Xinwei,Gong Tianxing,Leng Hongrui,Wei Shibo

Carboxymethyl cellulose (CMC) exhibits excellent biocompatibility and water retention, yet its high hydrophilicity often leads to excessive swelling and poor mechanical stability in physiological environments, limiting its utility in topical skin therapies. In this study, we developed a robust, chemically crosslinked CMC-based hydrogel loaded with curcumin (Cur@CL) using natural lysine as a non-toxic crosslinker. This green crosslinking strategy significantly reinforced the hydrogel network, achieving a swelling ratio of 3074 ± 226% and a compressive stress of 20.06 ± 1.0 kPa. The Cur@CL hydrogel demonstrated a sustained curcumin release profile of 83.98 ± 5.68% over 144 h, ensuring prolonged local bioavailability. In vitro, Cur@CL exhibited excellent cytocompatibility with L929 fibroblasts, accelerated fibroblast-associated wound closure, and reduced intracellular reactive oxygen species (ROS) levels by 62.97%. Furthermore, in a 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis (AD) mouse model, topical application of Cur@CL significantly alleviated clinical symptoms, reducing epidermal thickening by 71.5% compared with the DNCB-challenged model group, with no detectable systemic toxicity. This study presents a simple, tunable, and eco-friendly cellulose-based hydrogel platform for the effective, non-steroidal management of AD.

Antibacterial and preservation efficiency of photodynamic ice glaze driven by curcumin/β-cyclodextrin complex against Pacific white shrimp

Xiaowen Huang,Lu Chen,Huajian Ou,Yanyan Huang,Zhiyun Peng,Langhong Wang,Konglong Feng,Qiaohui Zeng,Jing Jing Wang

A novel photodynamic ice glaze with high antibacterial ability based on curcumin (Cur)/β-cyclodextrin (β-CD) inclusion complex was developed, and its active cold-chain preservation efficiency for Pacific white shrimp was investigated. Results showed that β-CD/Cur inclusion complex significantly enhanced the aqueous solubility and photostability of curcumin by facilitating the formation of a novel crystalline phase, which enabled efficient reactive oxygen species (ROS) generation in its frozen aqueous state. Under blue LED irradiation during frozen storage, the photodynamic ice exhibited broad-spectrum antibacterial ability against foodborne pathogens, notably with a reduction of >5.98 Log CFU/mL for Gram-positive Staphylococcus aureus and an undetectable level for Gram-negative Vibrio parahaemolyticus . Mechanistic studies confirmed that the generated ROS disrupted cellular structural integrity, and inflicted severe oxidative damage to bacterial DNA and proteins. During 15 days of frozen storage of shrimp, the photodynamic ice glaze slowed microbial proliferation (<5.20 Log CFU/g), inactivated endogenous proteases, retarded water migration, and maintained muscle quality. Therefore, the photodynamic ice glaze is a green and feasible strategy for improving the microbial safety and quality of aquatic products.

Ultrasound-assisted phosphorylation cantaloupe seed protein isolate-curcumin complexes prepared by pH shift treatment: Structural and functional properties

Jiankang Lu,Ruihua Zhang,Mingyan Ai,Chunlan Zhang,Shuting Li,Ruili Zhang,Weihua Wang,Zhiqiang Zhou,Yiming Jia,Zuoli Zhang,Shugang Li

The efficient loading of proteins on bioactive substances had been a research hotspot in the fields of food science and industry in recent years. The complex of ultrasound-assisted phosphorylated cantaloupe seed protein isolate (UP-CSPI) and curcumin (CUR) was prepared by pH shift method to investigate the effects of different concentrations of CUR on the structure and functional properties of UP-CSPI. The results showed that with the increase of CUR concentration, the zeta potential, the antioxidant activity and emulsifying performance of the complex improved. The interaction between CUR and UP-CSPI was a spontaneous reaction driven by Gibbs free energy (ΔG<0). The enthalpy change (ΔH) and entropy change (ΔS)>0, indicating mainly hydrophobic interaction. Furthermore, CUR caused changes in the secondary structure of the UP-CSPI, β-turns increased from 38.33 % to 45 %–47 %, random coils decreased from 25.85 % to 13 %–14 %. In conclusion, the binding of CUR to proteins through pH shift had solved the problem of CUR instability and also provided new insights for the development and application of plant protein-based delivery systems.

Curcumin-modified Mn-based metal–organic framework for synergistic sonodynamic and cascade anti-oxidation therapy of bacterial osteomyelitis

Zhiming Zhao,Mingjiang Luo,Liquan Li,Zhihong Xiao

The osteomyelitis induced by methicillin-resistant Staphylococcus aureus (MRSA) remains a formidable clinical challenge due to persistent bacterial infection, chronic inflammation, and impaired bone regeneration. Herein, a multi-functional nanoplatform is prepared by manganese-based porphyrinic metal-organic frameworks (PCN-222(Mn)) modified with curcumin (Cur) for treating bacterial osteomyelitis. The resulting PCN-222(Mn)-Cur nanoparticles exhibit effective sonodynamic antibacterial activity by rapid reactive oxygen species generation, which also has a synergistic effect (antimicrobial rate of 99.98 %) with long-term antibacterial efficiency of curcumin. Besides, the effective cascade anti-oxidation reaction of PCN-222(Mn) can be achieved by superoxide dismutase- and catalase-like activities owing to the MnN 4 active site. In a rat tibial osteomyelitis model, compared with antibiotic therapy, the ultrasound-activated PCN-222(Mn)-Cur platform exhibits superior antibacterial efficacy against MRSA, coupled with synergistic anti-inflammatory effects and promoted osteogenic regeneration capacity. In summary, this biosafe nanoplatform demonstrates excellent bacterial-killing, anti-inflammation, and osteogenesis, offering a prospect for treating refractory bacterial bone infections.

Water-solvable carbon dots derived from curcumin and citric acid with enhanced broad-spectrum antibacterial and antibiofilm activity

Fang Lu,Yurong Ma,Huibo Wang,Mengling Zhang,Bo Wang,Yue Zhang,Hui Huang,Fan Liao,Yang Liu,Zhenhui Kang

Multidrug-resistant bacterial infections are one of the most serious public health threats in the world, which bring an urgent need to discover novel antibiotics with enhanced broad-spectrum antibacterial activity . Curcumin , as a widely used natural product , has good anti-inflammatory and antioxidant properties; while, the bioavailability, bioactivity and antibacterial activity design are limited by its poor water-solubility. Here, to improve the bioactivity of curcumin, we show the fabrication of highly water-solvable carbon dots (CDs) by hydrothermal method from citric acid and curcumin. The CDs possess excellent broad-spectrum antibacterial activity and antibiofilm activity because they can do damage to the bacterial walls when they get into the bacteria. The bactericidal efficiency of CDs (375 μg/mL) achieves up to 100 % for Gram-negative bacteria ( Escherichia coli and Pseudomonas aeruginosa ). Additionally, for Gram-positive bacteria ( Staphylococcus aureus and Bacillus subtilis ), the bactericidal efficiency reaches 80 %. In addition, these CDs also can completely inhibit the formation of biofilm for the Gram-negative bacteria and the Gram-positive bacteria. This work has a significant meaning in studying new antibacterial nanomaterials derived from natural product and the treatment of infections.

Curcumin alleviates BPAF-induced ferroptosis in caprine endometrial epithelial cells through inhibition of endoplasmic reticulum stress and autophagy

Ming Liu,Cheng-Fang Gao,Jing-Jing Wang,Sheng-Ji Yang,Yin-Qing Li,Lei Sang,Li-Jun Huo

Bisphenol AF (BPAF) is widely used as a substitute for bisphenol A (BPA) in the plastics industry. However, it is known to cause reproductive toxicity in both humans and animal models. Curcumin, a polyphenolic compound from turmeric, is known for its potent anti-inflammatory and antioxidant effects. We previously showed that curcumin alleviates BPAF-induced apoptosis in caprine endometrial epithelial cells (EECs). However, the underlying mechanisms of BPAF toxicity remain unclear. The aim of this study was to investigate whether ferroptosis contributes to BPAF-induced injury in EECs and to assess the protective role of curcumin. We demonstrate that BPAF triggers ferroptosis in EECs: ferroptosis-related factors (COX2, FACL4, and NCOA4 ) were upregulated, and GSH content was increased. These effects were significantly reversed by the ferroptosis inhibitor Fer-1, which restored cell viability and reduced MDA accumulation. Mechanistically, BPAF-induced ferroptosis was autophagy-dependent, as evidenced by upregulated ATG5, Beclin1, and LC3, and enhanced autophagic flux. Inhibition of autophagy by CQ significantly attenuated ferroptosis and improved cell viability. Furthermore, ER stress acted as an upstream regulator, as its inhibitor 4-PBA alleviated both autophagy and ferroptosis. BPAF also disrupted cellular iron homeostasis by promoting NCOA4-mediated ferritinophagy, resulting in intracellular iron accumulation. Curcumin pretreatment alleviated BPAF-induced ferroptosis by suppressing ferritinophagy and restoring iron homeostasis. Moreover, activation of the PI3K/AKT/mTOR and Nrf2/HO-1 pathways may exert protective effects by suppressing autophagy and lipid peroxidation. In conclusion, this study indicates that ferroptosis is a key mechanism underlying BPAF-induced cytotoxicity in EECs. Curcumin protects against this damage by inhibiting ER stress and autophagy, providing a potential therapeutic strategy for BPAF-related uterine diseases.

Design of pH-sensitive methotrexate prodrug-targeted curcumin nanoparticles for efficient dual-drug delivery and combination cancer therapy

Jiajiang Xie,Zhongxiong Fan,Yang Li,Yinying Zhang,Fei Yu,Guanghao Su,Liya Xie,Zhenqing Hou

Sodium Alginate/carboxymethyl chitosan-CuO hydrogel beads as a pH-sensitive carrier for the controlled release of curcumin

Lu Wang,Xin Gang,Yu Xiao,Yi Ren,Jianhong Wang,Baolong Niu,Wenfeng Li

In this study, CMCS-CuO nanoparticles were added to the SA matrix by blending method to prepare SA/CMCS-CuO composite microspheres. The chemical structure and morphology of the composite beads were characterized. The results showed that there was a certain molecular interaction between CMCS-CuO NPs and SA matrix. The swelling experiment showed that with the increase of the mass of CMCS-CuO NPs, the maximum swelling ratio of gel microspheres decreased from 49.83% to 38.2%, and the time to reach the maximum swelling ratio also increased from 8 h to 11 h. Cytotoxicity test, hemolysis test, and cell migration test showed that the composite microspheres had good biocompatibility. Curcumin was added to the composite bead matrix by the blending method, and curcumin-loaded SA/CMCS-CuO composite beads were prepared by the crosslinking method. In vitro release experiments showed that SA/CMCS-CuO composite microspheres had good pH sensitivity, and the addition of CMCS-CuO NPs prolonged the in vitro release time of cur. It has certain application potential in the field of drug release.

Utilizing the Photodynamic Properties of Curcumin to Disrupt Biofilms in Cutibacterium acnes: A Promising Approach for Treating Acne

Nana Zheng,Yuanyuan Xie,Meng zhou,Yuzhen Liu,Haoxiang Xu,Rong Zeng,Chunping Wan,Min Li

Background : The treatment of acne vulgaris is often challenging due to the antibiotic resistance frequently observed in Cutibacterium acnes ( C.acnes ), a prevalent bacterium linked to this condition. Objective : The objective of this research was to examine the impact of curcumin photodynamic therapy (PDT) on the survival of C.acnes and activity of biofilms produced by this microorganism. Methods : Following the Clinical and Laboratory Standards Institute (CLSI) guidelines, we assessed the drug sensitivity of 25 clinical C.acnes strains to five antibiotics (erythromycin, clindamycin, tetracycline, doxycycline, minocycline) and curcumin by implementing the broth microdilution technique. In addition, we established C.acnes biofilms in a laboratory setting and subjected them to curcumin-PDT(curcumin combined with blue light of 180 J/cm 2 ). Afterwards, we evaluated their viability using the XTT assay and observed them using confocal laser scanning microscopy. Results : The result revealed varying resistance rates among the tested antibiotics and curcumin, with erythromycin, clindamycin, tetracycline, doxycycline, minocycline, and curcumin exhibiting resistance rates of 72%, 44%, 36%, 28%, 0%, and 100%, respectively. In the curcumin-PDT inhibition tests against four representative antibiotic-resistant strains, it was found that the survival rate of all strains of planktonic C. acnes was reduced, and the higher the concentration of curcumin, the lower the survival rate. Furthermore, in the biofilm inhibition tests, the vitality and three-dimensional structure of the biofilms were disrupted, and the inhibitory effect became more significant with higher concentrations of curcumin. Conclusion : The results emphasize the possibility of using curcumin PDT as an alternative approach for the treatment of C.acnes , especially in instances of antibiotic-resistant variations and infections related to biofilms.

Curcumin-Containing polyphosphazene nanodrug for Anti-Inflammation and nerve regeneration to improve functional recovery after spinal cord injury

Xiaochen Su,Xunan Jing,Wanting Jiang,Meng Li,Kai Liu,Menghao Teng,Daquan Wang,Lingjie Meng,Yingang Zhang,Wenchen Ji

The microenvironment of excessive inflammation and the activation of apoptotic signals are primary barriers to neurological recovery following spinal cord injury (SCI). Thus, long-lasting anti-inflammation has become an effective strategy to navigate SCI. Herein, a curcumin (CUR)-containing nanosystem (FCTHPC) with high drug loading efficiency was reported via assembling hydrophobic CUR into cross-linked polyphosphazene (PPZ), and simultaneous loading and coordinating with porous bimetallic polymers for greatly enhanced the water-solubility and biocompatibility of CUR. The nanosystem is noncytotoxic when directing its biological activities. By inhibiting the expression of pro-inflammatory factors (IL-1β, TNF-α and IL-6) and apoptotic proteins (C-caspase-3 and Bax/Bcl-2), which may be accomplished by activating the Wnt/β-catenin pathway, the versatile FCTHPC can significantly alleviate the damage to tissues and cells caused by inflammation and apoptosis in the early stage of SCI. In addition, the long-term in vivo studies had demonstrated that FCTHPC could effectively inhibit the formation of glial scars, and simultaneously promote nerve regeneration and myelination, leading to significant recovery of spinal cord function. This study emphasises the promise of the biocompatible CUR-based nanosystem and provides a fresh approach to effectively treat SCI.

Preparation of nano-sized multi-vesicular vesicles (MVVs) and its application in co-delivery of doxorubicin and curcumin

Qiankun Sun,Ju Liang,Yang Lin,Yunyun Zhang,Fuqing Yan,Wenlan Wu

Multi-vesicular vesicles (MVVs) offer structural advantages in terms of drug encapsulation and physiological stability. In this study, we address the challenge of preparing small-sized MVVs for drug delivery. The nano-sized MVVs (~120   nm) loaded with doxorubicin (DOX) and curcumin (CUR) (DOX/ [email protected] ) were successfully prepared using a glass bead combined with a thin film dispersion method. Transmission electron microscopy (TEM) and dynamic light scattering (DLS) analysis confirmed the independent non-homocentric vesicle structures of DOX/ [email protected] with homogeneous particle sizes. The experimental results showed high encapsulation rates of DOX and CUR in DOX/ [email protected] , reaching 82.5±0.75% and 85.9±0.69%, respectively. Moreover, the MVVs exhibited good biosafety and sustained release properties. Notably, the bioavailability of DOX and CUR in DOX/ [email protected] was enhanced compared to free DOX and CUR, with increases of 4.2 and 2.1 times, respectively. And the half-life of DOX and CUR was extended by 10 times in DOX/ [email protected] In vivo antitumor experiments demonstrated that nano-sized DOX/ [email protected] significantly improved the antitumor activity while reducing the toxic side effects of DOX. Overall, the successful preparation of nano-sized DOX/ [email protected] and their potent and low-toxic antitumor effects provide a critical experimental reference for the combined antitumor therapy of MVVs and liposomes.

Effects of Different Molecular Weight Oxidized Dextran as Crosslinkers on Stability and Antioxidant Capacity of Curcumin-Loaded Nanoparticles

Dongyan Shen,Hongzhou Chen,Mingwei Li,Ling Yu,Xiangfei Li,Huawei Liu,Qiaobin Hu,Yingjian Lu

Curcumin is a polyphenolic compound that has been widely investigated for its health benefits. However, the clinical relevance of curcumin is limited due to its low water solubility and inefficient absorption. Therefore, curcumin is often encapsulated in nanocarriers to improve its delivery and function. In this study, composite nanoparticles composed of stearic acid-modified chitosan (SA-CS) and sodium caseinate (NaCas) were formed using sodium periodate-oxidized dextran with different molecular weights as a crosslinking agent. The effects of oxidized dextran (Odex) with different molecular weights on the composite nanoparticles were compared. The optimal SA-CS/NaCas/Odex composite nanoparticle (NPO) was obtained using an Odex (150 kDa)-to-SA-CS mass ratio of 2:1. Its size, polydispersity index (PDI), and zeta potential (ZP) were 130.2 nm, 0.149, and 25.4 mV, respectively. The particles were highly stable in simulated gastric fluid (SGF) in vitro, and their size and PDI were 172.3 nm and 0.263, respectively. The encapsulation rate of NPO loaded with curcumin (Cur-NPO) was 93% under optimal ultrasonic conditions. Compared with free curcumin, the sustained release of Cur-NPO significantly reduced to 17.9%, and free-radical-scavenging ability improved to 78.7%. In general, the optimal prepared NPO exhibited good GI stability and has potential applications in the formulation of orally bioactive hydrophobic drugs.Keywords:curcumin;modified chitosan;oxidized dextran;nanoparticles;stability;antioxidant capacity