Journal of Physical Chemistry C
·
2018
·
IF 4.484
Xin Liu,Hongjian Zhang,Binbin Zhang,Jiangpeng Dong,Wanqi Jie,Yadong Xu
Methylammonium (MA) lead hybrid perovskite single crystal recently received attention as a potential radiation detection material. Here, we report the MAPbBr3 bulk crystals grown by the modified antisolvent vapor-assisted crystallization method. The growth rate is determined by diluting the antisolvent, which results in the average size of MAPbBr3 crystals to significantly increase from 2 × 2 × 1 to 15 × 15 × 5 mm3. The morphology evolution of MAPbBr3 crystals, which is contributed by the growth anisotropy, has been discussed according to the molar ratios of precursors and the bond theory. The resulting centimeter-sized MAPbBr3 crystals exhibit high resistivity (5.6 × 108 Ω·cm) at room temperature. The mobility–lifetime (μτ) products are measured under 241Am (5.48 MeV) α particles irradiation, with the values of 2.2 × 10–4 and 4.2 × 10–4 cm2/V for electrons and holes, respectively. Simultaneously, the electrons and holes mobility are estimated to be 24.6 and 59.7 cm2/(V·s), respectively, using the α-source-induced transient waveforms.
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FOOD HYDROCOLLOIDS
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2018
·
IF 5.089
Hao Cheng,Zheng Fang,Wusigale,Amr M. Bakry,Yantao Chen,Li Liang
Clarification of the mechanism of protein-ligand interaction is important for the encapsulation of bioactive components. Trans -resveratrol is labile to convert to cis -isomer during fermentation and storage, but the cis -isomer interaction with proteins has not yet gained as much attention as that of trans -isomer. The influence of resveratrol's isomerization on interaction with ligand-binding proteins was investigated here. Cis -resveratrol exhibited higher affinity for bovine serum albumin than trans -isomer, but the isomerization to cis -isomer decreased the polyphenol affinity for α-lactalbumin and β-lactoglobulin. For each protein, trans - and cis -resveratrol shared the same optimal docking site but showed clear diversity in the second best site. Protein ternary complex could be formed when trans -/ cis -resveratrol were added in the sequence. Moreover, photostability of resveratrol and antioxidant activity of the polyphenol-protein mixtures were also discussed. The results gathered here should provide further insight into protein-polyphenol interactions and be useful for the development of protein-based carriers for the polyphenols.
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mAbs
·
2018
·
IF 5.165
De-zhi Li,Bing-nan Han,Rui Wei,Gui-yang Yao,Zhizhen Chen,Jie Liu,Terence C.W. Poon,Wu Su,Zhongyu Zhu,Dimiter S. Dimitrov,Qi Zhao
Site-specific conjugation of small molecules to antibody molecules is a promising strategy for generation of antibody-drug conjugates. In this report, we describe the successful synthesis of a novel bifunctional molecule, 6-(azidomethyl)-2-pyridinecarboxyaldehyde (6-AM-2-PCA), which was used for conjugation of small molecules to peptides and antibodies. We demonstrated that 6-AM-2-PCA selectively reacted with N-terminal amino groups of peptides and antibodies. In addition, the azide group of 6-AM-2-PCA enabled copper-free click chemistry coupling with dibenzocyclooctyne-containing reagents. Bifunctional 6-AM-2-PCA mediated site-specific conjugation without requiring genetic engineering of peptides or antibodies. A key advantage of 6-AM-2-PCA as a conjugation reagent is its ability to modify proteins in a single step under physiological conditions that are sufficiently moderate to retain protein function. Therefore, this new click chemistry-based method could be a useful complement to other conjugation methods.
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CATALYSIS LETTERS
·
2018
·Vol. 148
·期 11
·3492-3501
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IF 2.911
Wang Xueyuan,Chen Li,Huang Deqi,Yue Jinfang,Luo Zhichen,Zeng Tao
Abstract The views on catalytic activity of zeolite in isomerization of α -pinene have not came to an agreement. Thus, the key active substance in zeolite was further studied through experiments. H-mordenite was treated in three ways: (i) calcination under nitrogen flows, (ii) calcination under oxygen flows, (iii) calcination under nitrogen flows after alkali treatment. The properties of modified zeolites were investigated by methods of SEM, EDS, XRD, nitrogen adsorption, NH 3 -TPD, Py-IR, and 29 Si and 27 Al MAS NMR. The results showed that the zeolites produced mesoporous after 0.4M NaOH solution soaking and calcinations above 500 °C, but it had a slight effect on the change of micropore volumes. Comparison of zeolite acid sites amount under different roasting temperature by NH 3 -TPD showed that the decrease of acid sites amount was mainly related to the roasting temperature. Py-IR and 27 Al MAS NMR experiment revealed that Lewis acid was produced by framework aluminum. Finally, Analysis on the influencing factors we studied of H-mordenite catalytic activity in this paper suggested that, in isomerization of α -pinene, the relative concentration of six-coordination aluminum in zeolite is the dominant factor of provided catalytic activity of H-mordenite. Graphical abstract
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CELLULAR AND MOLECULAR NEUROBIOLOGY
·
2019
·Vol. 39
·期 7
·1017-1028
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IF 3.811
Hua Jun,Yin Nuo,Xu Shi,Chen Qiang,Tao Tingting,Zhang Ji,Ding Jianhua,Fan Yi,Hu Gang
The accumulation of aggregated forms of the α-Synuclein (α-Syn) is associated with the pathogenesis of Parkinson’s disease (PD), and the efficient clearance of aggregated α-Syn represents a potential approach in PD therapy. Astrocytes are the most numerous glia cells in the brain and play an essential role in supporting brain functions in PD state. In the present study, we demonstrated that cultured primary astrocytes engulfed and degraded extracellular aggregated recombinant human α-Syn. Meanwhile, we observed that the clearance of α-Syn by astrocytes was abolished by proteasome inhibitor MG132 and autophagy inhibitor 3-methyladenine (3MA). We further showed that intracellular α-Syn was reduced after ginkgolide B (GB) and bilobalide (BB) treatment, and the decrease was reversed by MG132 and 3MA. More importantly, GB and BB reduced indirect neurotoxicity to neurons induced by α-Syn-stimulated astrocytic conditioned medium. Together, we firstly find that astrocytes can engulf and degrade α-Syn aggregates via the proteasome and autophagy pathways, and further show that GB and BB enhance astrocytic clearance of α-Syn, which gives us an insight into the novel therapy for PD in future.
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Marine Drugs
·
2019
·Vol. 17
·期 6
·332
·
IF 3.772
Zhongbin Cheng,Yuanli Li,Wan Liu,Lijun Liu,Jie Liu,Wangjun Yuan,Zhuhua Luo,Wei Xu,Qin Li
Three new butenolide derivatives, namely aspernolides N–P (1–3), together with six known analogues (4–9), were isolated from the ethyl acetate (EtOAc) extract of the deep sea-derived fungusAspergillus terreusYPGA10. The structures of compounds1–3were determined on the basis of comprehensive analyses of the nuclear magnetic resonance (NMR) and mass spectroscopy (MS) data, and the absolute configurations of1and2were determined by comparisons of experimental electronic circular dichroism (ECD) with calculated ECD spectra. Compound1represents the rare example ofAspergillus-derived butenolide derivatives featured by a monosubstituted benzene ring. Compounds6–9exhibited remarkable inhibitory effects againstα-glucosidase with IC50values of 3.87, 1.37, 6.98, and 8.06 μM, respectively, being much more active than the positive control acarbose (190.2 μM).Keywords:Aspergillus terreusYPGA10;deep-sea-derived fungus;butenolide derivatives;α-glucosidase
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CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2019
·Vol. 35
·期 2
·319-324
·
IF 1.071
Wang Junlin,Yi Yongjie,Li Mengjia,Chang Yue,Zha Fei
Graphitic carbon nitride(g-C 3 N 4 ) microspheres supported α-FeO(OH) hybrids[α-FeO(OH)/g-C 3 N 4 ] were prepared by means of a self-assembly method in deionized water. By UV-visible diffuse reflectance spectroscopy, it has been confirmed that α-FeO(OH)/g-C 3 N 4 has a wider absorption range than g-C 3 N 4 . The feature of α-FeO(OH)/g-C 3 N 4 can be attributed to the efficient separation of the electron-hole pairs with photoluminescence spectra. The degradation rate of methyl orange(MO) is up to 99% under the optimal conditions of 110 min, initial concentration of 30 mg/L, an α-FeO(OH)/g-C 3 N 4 dosage of 15 mg as well as visible light. The mechanism for this photocatalytic reaction was proposed, with hydroxyl radicals being a major active catalytic species.
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ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Heng Zhang,Zhen Wang,Xiaoqing Yang,Zi-Long Li,Linhao Sun,Jimei Ma,Hong Jiang
A sensitive nanocomplex probe prepared from fluorescent polydopamine nanoparticles (F-PDA) and cobalt oxyhydroxide (CoOOH) nanosheets was established for the determination of α-glucosidase activity. In this detection system, the fluorescence of F-PDA was firstly quenched by CoOOH nanosheets based on fluorescence resonance energy transfer (FRET). Subsequently, ascorbic acid was produced from 2-O-α- d -glucopyranosyl- l -ascorbic acid which was selectively hydrolyzed by α-glucosidase. CoOOH was reduced to Co 2+ by the released ascorbic acid, which resulted in the recovery of F-PDA nanoparticles fluorescence. In consequence, α-glucosidase activity was determined by the fluorescence recovery degree of the F-PDA nanoparticle. This fluorescent method showed a good linear relationship with the activity of α-glucosidase from 2 to 80 U L −1 and low detection limit of 1.65 U L −1 (S/N = 3). This fluorescence probe with high selectivity and sensitivity demonstrated a remarkable applicability in human serum samples and provided an alternative for α-glucosidase inhibitors screening in the discovery of anti-diabetes drugs.
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Journal of Environmental Sciences
·
2017
·
IF 2.865
Guoqiang Zhang,Hongbo Fu,Jianmin Chen
The α-pinene ozonolysis under the different environmental conditions were observed in a smog chamber. The second-order rate constant ( k ) was determined to be (7.25 ± 0.06) × 10– 17 cm 3 /(molecule·sec) under 20% of relative humidity (RH) and room temperature. RH showed a marked influence on the α-pinene ozonolysis. The value of k increased with RH increase, which was 1.6 times faster at RH = 80% than that at RH = 20%. Additionally, the value of k apparently changed in the presence of the aerosol particles. The diesel soot increased the k value. The fly ash prohibited the reaction, however, H 2 SO 4 -treated fly ash promoted the reaction. The information of products gained using FT-IR and SPAMS showed that pinonic acid, 10-hydroxy-pinonic acid and pinic acid could be generated during the α-pinene ozonolysis. Water molecules could take part in the formation of the products, and play a vital role in the degradation of α-pinene. The atmospheric residence time calculation showed that the ozonolysis in the atmosphere is an important way of the α-pinene consumption as compared to that reacted with OH during daytime. The results suggested that the degradation of α-pinene via the ozonization in the atmosphere may be affected greatly by RH, as well as the presence of aerosol particles. The ozonolysis reaction may be an important way of the α-pinene consumption during daytime.
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INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2017
·Vol. 52
·期 12
·2596-2603
·
IF 1.64
Lin Ge,Jingcong Xie,Tao Wu,Shanshan Zhang,Linguo Zhao,Gang Ding,Zhenzhong Wang,Wei Xiao
Summary A novel α-L-rhamnosidase was isolated and purified from Aspergillus oryzae NL-1. The enzyme was purified 13.2-fold by ultrafiltration, ion exchange and gel filtration chromatography with an overall recovery of 6.4% and specific activity of 224.4 U/mg, and the molecular mass of its subunit was approximately 75 kDa. Its optimal temperature and pH were 65 °C and 4.5, respectively. The enzyme was stable in the pH range 3.5–7.0, and it showed good thermostability at higher temperatures. The K M , kcat and kcat/K M values were 5.2 m m , 1624 s −1 and 312 s −1 m m −1 using p NPR as substrates, respectively. Moreover, the enzyme exhibited transglycosylating activity, which could synthesise rhamnosyl mannitol through the reactions of transglycosylation with inexpensive rhamnose as the glycosyl donor. Our findings indicate that the enzyme has potential value for glycoside synthesis in the food industry.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Zhuangzhuang Huang,Junru Zhou,Jialing Wang,Sheng Xu,Cheng Cheng,Jiangfeng Ma,Zhen Gao
The industrial applications of enzymes are limited due to the activity-stability trade-off, which implies that the improvement of thermostability often accompanies decreased activity. This study presents a dual-strategy approach to simultaneously improve the catalytic efficiency and thermostability of α-galactosidase galV from Anoxybacillus vitaminiphilus WMF1. Our integrated method combines computational analysis with enzyme property prediction to selectively target and modify the catalytic region and residues that are distant from the active site. We identified and experimentally validated mutations that improve activity without compromising stability and further increased thermostability through additional distant-site mutations. The resulting mutant enzyme variant N549Q/T550N/Y634F demonstrated a 6.2-fold increase in catalytic efficiency and a 3.2-fold improvement in the half-life at 65 °C. Molecular dynamics (MD) simulations supported the structural basis for the observed enhancements. This approach offers a refined strategy for engineering α-galactosidases with improved industrial applicability, overcoming the traditional trade-offs between enzyme activity and stability. Hydrolytic activity toward raffinose family oligosaccharides (RFOs) was validated using soymilk as a model substrate, demonstrating significant practical potential.
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ELECTROCHIMICA ACTA
·
2024
·
IF 5.5
Shiyu Jia,Ziyang Liu,Yuanpan Gao,Ruoran Zhang,Rui Zhu,Shanshan Gao,Peipei Guan,Xuanran Geng,Ke Tian,Shouli Bai
The design of photoanode materials to achieve efficient water splitting is a current research hotspot in photoelectrochemical (PEC). In this work, we designed and prepared an α-Fe 2 O 3 /BiVO 4 heterostructure modified by carbon quantum dots ((α-Fe 2 O 3 /BiVO 4 /CQDs)) as the photoanode. The modification of CQDs can broaden the sunlight absorption ability and dramatically enhance the water oxidation kinetics of α-Fe 2 O 3 /BiVO 4 /CQDs. The optimized photoanode demonstrated a photocurrent density of 1.32 mA·cm −2 at 1.23 V vs. RHE, which is 2.2 times than pristine α-Fe 2 O 3 (0.6 mA·cm −2 ) photoanode. The IPCE value above 29.4 % at 400 nm, and the onset potential is negatively shifted by 243 mV for α-Fe 2 O 3 /BiVO 4 /CQDs. The improved performance is mainly due to the α-Fe 2 O 3 /BiVO 4 heterojunction to achieve effective separation of photogenerated carriers in the bulk phase and CQDs to improve light utilisation and OER kinetics of the photoanode.
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危险品化学品经营许可证（带存储）