Micro & Nano Letters
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2019
·Vol. 14
·期 11
·1151-1156
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IF 0.975
Min Shi,Yanhong Li,Hongtao Cui
A facile strategy for the preparation of composites with homogeneous distribution of multi-walled carbon nanotubes (MWCNTs) in Ni(OH) 2 was proposed. The electrostatic attraction between positively charged Ni(OH) 2 monolayer nanosheets and negatively charged MWCNTs was utilised to self-assemble the composites. The results of electrochemical measurement on the composite containing 30 wt% MWCNTs indicated that the homogeneous composition of MWCNTs in composite significantly reduced the electrical resistance and promoted the electrochemical utilisation of Ni(OH) 2 . As a result, the composite presented high electrochemical performance: having specific capacitance of 1568.8 F g −1 at low current density of 2.4 A g −1 and keeping a relatively high specific capacitance of 781.8 F g −1 at high current density of 81.7 A g −1 . Based on the results in this works, it is hoped that the adopted strategy could provide a promising idea for the preparation of composites with homogeneous distribution of individual components.
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PROTEIN EXPRESSION AND PURIFICATION
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2022
·
IF 2.025
Yi Rong,Chaofeng Zhang,Wen-Chao Gao,Cheng Zhao
The main protease (M pro ) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) plays a vital role in viral replication. To study the function of M pro and screen inhibitors targeting M pro , it is necessary to prepare high-purity and high-activity M pro . In this study, four types of SARS-CoV-2 M pro s containing different termini were prepared, and their activities were determined successfully. The results showed that the activity of wild-type (WT) M pro was the highest, and the additional residues at the N-terminus but not at the C-terminus had a major effect on the enzyme activity. To explain this, the alignment of structures of different forms of M pro was determined, and the additional residues at the N-terminus were found to interfere with the formation of the substrate binding pocket. This study confirms the importance of the natural N-terminus to the activity of M pro and suggests that WT-GPH 6 (M pro with eight additional residues at the C-terminus) can be used as a substitute for authentic M pro to screen inhibitors. In short, this study provides a reference for the expression and purification of new coronaviruses confronted in the future.
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INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
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2022
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IF 7.139
Yuyu Li,Fang Luo,Yuhua Xie,Chaofeng Chang,Ming Xie,Zehui Yang
Urea oxidation reaction (UOR) is a promising substitution of oxygen evolution reaction (OER) in overall water splitting technology because of the lower theoretical potential; thereby, a win-win benefit is achieved due to the simultaneous hydrogen generation and water purification . The efficient UOR catalysis substantially depends on the exposed active sites and mass transfer resistance, especially at high current density to meet the requirement of industrial water splitting technology. In this work, we have synthesized α-Ni(OH) 2 porous nanoflowers (α-Ni(OH) 2 –PNF) using ZIF-67 as template. Ascribing to the Kirkendall effect , nanopores are created on α-Ni(OH) 2 nanosheets benefiting for the formation of abundant oxygen vacancies as well as drop-off in mass transfer resistance during UOR catalysis. Meanwhile, the binding strength between active site and CO 2 , rate-determining step of UOR, is weakened by oxygen vacancies; as a result, only 1.477 V vs. RHE is demanded for α-Ni(OH) 2 –PNF to catalyze UOR with 100 mA cm −2 , 67 mV lower than α-Ni(OH) 2 nanosheets. Besides, an exceptional stability is also achieved for α-Ni(OH) 2 –PNF with negligible degradation during 20 h catalysis.
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JOURNAL OF CHEMICAL THERMODYNAMICS
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2022
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IF 3.269
Huimin Li,Yueru Wang,Yidan Wu,Yu Li,Tao Li,Baozeng Ren
The solubility of 2-naphthoxyacetic acid in methanol, ethanol, n -propanol, i-propanol, i-butanol, n -pentanol, methyl acetate, ethyl acetate, n -propyl acetate, i-propyl acetate, acetonitrile, and acetone was determined with the gravimetric method under the barometric pressure in the temperature range of 278.15 K to 323.15 K. The experimental results indicate that the solubility of 2-naphthoxyacetic acid in the twelve solvents was positively related to temperature over the whole range of temperature studied. Six mathematical models were used to fit the experimental results, including modified Apelblat equation, UNIQUAC model, λh equation, NRTL model, Two-Suffix Margules model and Jouyban model. The best correlation results were shown using the modified Apelblat model. The Hansen Solubility Parameter (HSP) was used to discuss the order of solubility of 2- naphthoxyacetic acid in the studied solvents. In addition, the thermodynamic properties during the dissolution of 2-naphthoxyacetic acid were analyzed and discussed according to the Van't Hoff equation.
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EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
·
2016
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IF 3.902
Yu-Ou Teng,Hong-Ye Zhao,Jing Wang,Huan Liu,Mei-Le Gao,Yao Zhou,Kai-Lin Han,Zhen-Chuan Fan,Yong-Min Zhang,Hua Sun,Peng Yu
A series of novel di- or trisubstituted isatin derivatives were designed and synthesized in 5–6 steps in 25–45% overall yields. Their structures were confirmed by 1H NMR and 13C NMR as well as LC-MS. The anticancer activity of the fourty-three new isatin derivatives against human T lymphocyte cells Jurkat was evaluated by MTT assay in vitro . SAR study suggested that the combination of 1-benzyl and 5-[ trans -2-(methoxycarbonyl)ethen-1-yl] substitution greatly enhanced their cytotoxic activity. Among them, compound 2h was shown to have a significant cytotoxic activity with an IC50 value of 0.03 μM, more than 330-fold higher than that of it's mother molecule isatin. Investigation of the cell morphology changes and annexin-V/PI staining study demonstrated that compound 2h inhibited the proliferation of Jurkat cells by inducing apoptosis. Since compound 2h induced the dissipation of mitochondrial membrane potential and the activation of caspase-3, it was obvious that compound 2h inhibited the proliferation of Jurkat cells through the mitochondrial apoptotic pathway. Other than this, compound 2h exerted inhibition effect to many other tumor cells and only showed weak cytotoxic to human normal cells suggesting that compound 2h possessed a broad range of anticancer spectrum and high safety to normal cells.
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Frontiers in Bioscience-Landmark
·
2023
·Vol. 28
·期 11
·311
·
IF 3.1
Qing Zhu,Yuanshan Han,Ying He,Yilan Fu,Hui Yang,Yun Chen,Yingrui Shi
Background: Breast cancer-related depression (BCRD) is strongly associated with BC and increases recurrence and mortality. This study investigated the role of kaempferol in the pathogenesis of BCRD and its underlying mechanism. Methods: 4T1 mouse BC cells were treated with corticosterone (Cort) in vitro to develop a neuronal injury model, and a BCRD mouse model was established by injecting 4T1 cells and Cort. The effects of kaempferol on 4T1 cells and BCRD models were measured by behavioral tests, Cell Counting Kit-8 assay, wound healing assay, colony formation assay, Western blot analysis, quantitative real-time PCR, hematoxylin and eosin staining, enzyme-linked immunosorbent assay, and immunofluorescence. BCRD cells were transfected with the cyclo-oxygenase-2 (COX-2) overexpression plasmid to study the role of the COX-2/prostaglandin E2 (PGE2) axis in the anti-BCRD activity of kaempferol. The connection between kaempferol and COX-2 was analyzed by molecular docking. Results: Kaempferol reduced the viability, migration, and clones of 4T1 cells and inhibited BC growth and depression-like behavior in mice. Kaempferol alleviated inflammation in BCRD, decreased interleukin 1 beta (IL-1β) and IL-6 levels, and increased transforming growth factor beta 1 (TGF-β1) and IL-10 levels. In addition, kaempferol elevated the levels of serotonin, dopamine, and norepinephrine and the amount of 5-Bromo-2′-deoxyuridine/neuronal nuclei-positive cells. Kaempferol downregulated COX-2 and PGE2, and kaempferol could dock with the protein structure of COX-2. Overexpression of COX-2 reduced BCRD viability, upregulated IL-1β and IL-6 levels, and downregulated TGF-β1 and IL-10 expression. Overexpression of COX-2 reversed the protective effects of kaempferol. Conclusion: Kaempferol exerted anti-BCRD effects, at least in part by inhibiting the COX-2/PGE2 pathway, which regulates neuroinflammation, neurotransmitter imbalance, and defective neurogenesis. Therefore, kaempferol may be a promising candidate active ingredient for treating BCRD.
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BIOCHEMICAL PHARMACOLOGY
·
2023
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IF 5.8
Luomin Lin,Yuwei Huang,Jinyi Wang,Xiaocao Guo,Fangjun Yu,Di He,Chuanbin Wu,Lianxia Guo,Baojian Wu
CYP2A5 , an enzyme responsible for metabolism of diverse drugs, displays circadian rhythms in its expression and activity. However, the underlying mechanisms are not fully established. Here we aimed to investigate a potential role of CRY1/2 (circadian clock modulators) in circadian regulation of hepatic CYP2A5. Regulatory effects of CRY1/2 on CYP2A5 were determined using Cry1 -null and Cry2 -null mice, and validated using AML-12, Hepa1-6 and HepG2 cells. CYP2A5 activities both in vivo and in vitro were assessed using coumarin 7-hydroxylation as a probe reaction. mRNA and protein levels were detected by qPCR and western blotting , respectively. Regulatory mechanism was studied using a combination of luciferase reporter assays, chromatin immunoprecipitation (ChIP) and co-immunoprecipitation (Co-IP). We found that ablation of Cry1 or Cry2 in mice reduced hepatic CYP2A5 expression (at both mRNA and protein levels) and blunted its diurnal rhythms. Consistently, these knockouts showed decreased CYP2A5 activity (characterised by coumarin 7-hydroxylation) and a loss of its time-dependency, as well as exacerbated coumarin-induced hepatotoxicity. Cell-based assays confirmed that CRY1/2 positively regulated CYP2A5 expression and rhythms. Based on combined luciferase reporter, ChIP and Co-IP assays, we unraveled that CRY1/2 interacted with E4BP4 protein to repress its inhibitory effect on Cyp2a5 transcription and expression . In conclusion, CRY1/2 regulate rhythmic CYP2A5 in mouse liver through repression of E4BP4. These findings advance our understanding of circadian regulation of drug metabolism and pharmacokinetics .
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2023
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IF 5.2
Yin Xie,Xiaowei Xu,Yan Cui,Liang Qiao,Kebin Chi,Dejun Shi,Yang Bai,Zhijie Wu,Shengbao He
Solvent is vital in determining the colloidal chemistry of perovskite ink. A depth understanding on the colloidal chemistry is essential to fine-regulate the crystallization of perovskite film. A low-polar solvent, 2-methoxyethanol (2-Me), shows growing potential than the traditional high-polar solvents in scaling-up perovskite solar cells, owing to the fast evaporation rate and the ability to form perovskite films without anti-solvent. However, as a relatively new solvent, the colloidal chemistry has not been fully understood. Here, the colloidal chemistry of fresh and aged perovskite ink using 2-Me as the solvent is investigated. The as-composed perovskite ink is characteristic of weakly bonded I - ions and the existence of cluster complexes. In the fresh perovskite ink, the weakly bonded I - ions enhance the dissolution of methylammonium (MA + ), and also induce the formation of I 3 - ions in the diluted or aged solution. Both iodoplumbate species and cluster complexes are dispersed by MA + ions in the solution, however, the former transforms to clusters after aging. These changes results in increased crystal size and non-uniform coverage in the perovskite film. These findings provide valuable insights into the optimization of perovskite ink using 2-Me as the solvent.
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MOLECULES
·
2023
·Vol. 28
·期 19
·6777
·
IF 4.6
Zhaoyu Zhang,Yurong Zhao,Zhang Hu,Zhenyu Si,Ziming Yang
The widespread prevalence of infectious bacteria is one of the greatest threats to public health, and consequently, there is an urgent need for efficient and broad-spectrum antibacterial materials that are antibiotic-free. In this study, 2-pyridinecarboxaldehyde (PCA) was grafted onto chitosan (CS) and the modified CS coordinated with silver ions to prepare PCA-CS-Ag complexes with antibacterial activity. To obtain complexes with a high silver content, the preparation process was optimized using single-factor experiments and response surface methodology. Under the optimal preparation conditions (an additional amount of silver nitrate (58 mg), a solution pH of 3.9, and a reaction temperature of 69 °C), the silver content of the PCA-CS-Ag complex reached 13.27 mg/g. The structure of the PCA-CS-Ag complex was subsequently verified using ultraviolet–visible spectroscopy, Fourier-transform infrared spectroscopy, proton nuclear magnetic resonance spectroscopy, and thermogravimetric analysis. Furthermore, three possible complexation modes of the PCA-CS-Ag complex were proposed using molecular mechanics calculations. The results of the antibacterial assay in vitro showed that the PCA-CS-Ag complex exhibited strong antibacterial activity against both Gram-positive and Gram-negative bacteria, exerting the synergistic antibacterial effect of modified chitosan and silver ions. Therefore, the PCA-CS-Ag complex is expected to be developed as an effective antibacterial material with promising applications in food films, packaging, medical dressings, and other fields.Keywords:modified chitosan;silver;complex;response surface methodology;antibacterial
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FUEL
·
2023
·
IF 7.4
Fangfang Shang,Zhuo Li,Hendao Quan,Xiaoxun Ma
Constructing electrode-electrolyte interphase and enhancing wettability between separator and electrolyte are vital to electrochemical performances of Lithium-metal batteries, especially for suppressing the growth of Li dendrites. In this work, 2-Trifluoroacetyl furan is developed as the multifunctional additive to inhibit Li dendrite growth, and then enhance the cycling time and rate performance of Li||LiNi 0.6 Co 0.2 Mn 0.2 O 2 (NCM622) cells. TFFE additive can enhance the Li ion flowing efficiency by improving wettability between separator and electrolyte and providing more channel of Li ions. Furthermore, TFFE can not only form solid electrolyte interphase (SEI) on the surface of Li anode, but also forms cathode electrolyte interphase (CEI) on the surface of NCM622 electrode. Finally, TFFE could stabilize the LiPF 6 -based electrolyte by removing hydrogen fluoride (HF) and H 2 O, protecting the generated SEI/CEI layer. As a result, introducing 1.0 wt% TFFE additive, the Li||Li symmetric cells could achieve stable cycling for 780 h at 0.5 mA cm −2 , which is higher than that in baseline electrolyte (350 h). And, Li||NCM622 cells in the 1.0 wt% TFFE-contained electrolyte displays remarkably enhanced capacity of 90 % almost after 200 cycles.
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JOURNAL OF ORGANOMETALLIC CHEMISTRY
·
2023
·
IF 2.3
Lin Tan,Ce Xu,Zhen Li,Yirong Wang,Xianghong Li,Bingguang Zhang
A cyclometalated ruthenium complex ( 1 ) with 2-phenylbenzimidazole as the ligand was synthesized and used as a colorimetric chemosensor to detect Hg 2+ and Cu 2+ . It is interesting that the presence of Hg 2+ caused a blue-shifted absorption of 1 from 560 to 450 nm resulting in a solution color change from red to yellow, while the addition of Cu 2+ resulted in an attenuated absorption along with the solution color changing from red to colorless. Their corresponding detection limits were calculated to be 0.25 µM for Hg 2+ and 0.45 µM for Cu 2+ , respectively. Moreover, the slips of paper were dyed with complex 1 and then successfully used to recognize Hg 2+ and Cu 2+ by naked eyes, respectively. Furthermore, 1 was also used to determine Hg 2+ and Cu 2+ in some spiked water samples, respectively.
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FUEL
·
2023
·
IF 7.4
Shanlong An,Xin Huang,Nuo Li,Qiangwei Li,Rujie Wang,Tieyue Qi,Lidong Wang
The CO 2 capture process based on amine absorption–regeneration is currently the most widely used technology, and the performance of absorbent is the key to this process. Biphasic absorbents have attracted considerable interest because of their strong potential to reduce the regeneration energy consumption and equipment investment costs. The mixed solution of diethylenetriamine (DETA) and 2-diethylaminoethanol (DEEA) has been proven to be a biphasic absorbent, but there is still a lack of comprehensive performance evaluation data. In this study, the ratio of the DETA/DEEA solution was optimized as 2 M DETA + 3 M DEEA, and its phase separation property, reaction mechanism, mass transfer kinetics, and desorption thermodynamics were investigated under various conditions. The comprehensive performance of the solution was then evaluated by further analyzing its oxidative degradation, thermal degradation, and metal corrosion characteristics. On this basis, the capital cost and operation cost of using DETA/DEEA biphasic absorbent were analyzed. Under typical coal-fired flue gas (CO 2 concentration 12%), because of the high CO 2 absorption capacity and excellent phase separation property, the minimal desorption energy consumption of the DETA/DEEA absorbent decreased to 2.14 GJ/t CO 2 , a 43.7% decrease compared with that of 5 M monoethanolamine (MEA). Under the same experimental conditions, DETA/DEEA exhibited an oxidative degradation rate of only 2.71%, which was 20.7% of 5 M MEA. In addition, the metal corrosion rate of DETA/DEEA was one order of magnitude lower than that of 5 M MEA. However, under a high CO 2 loading, its thermal degradation resistance needed to be improved, and it was clear that the rates of oxidative degradation, metal corrosion, and thermal degradation of the CO 2 rich lower phase were higher than those of the CO 2 lean upper phase. The capital cost and operational capture cost of the DETA/DEEA biphasic process were approximately $107.8/t CO 2 and $31.6/t CO 2 , respectively, which were 2.5% and 36.4% lower than those of the MEA process. These findings provide a theoretical basis for the industrial application of DETA/DEEA.
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