eLife
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2022
·
IF 8.713
Weizhao Chen,Qiongping Zheng,Qiaoying Huang,Shanshan Ma,Mingtao Li
Lineage reprogramming of resident glial cells to dopaminergic neurons (DAns) is an attractive prospect of the cell-replacement therapy for Parkinson’s disease (PD). However, it is unclear whether repressing polypyrimidine tract binding protein 1 (PTBP1) could efficiently convert astrocyte to DAns in the substantia nigra and striatum. Although reporter-positive DAns were observed in both groups after delivering the adeno-associated virus (AAV) expressing a reporter with shRNA or CRISPR-CasRx to repress astroglial PTBP1, the possibility of AAV leaking into endogenous DAns could not be excluded without using a reliable lineage-tracing method. By adopting stringent lineage-tracing strategy, two other studies show that either knockdown or genetic deletion of quiescent astroglial PTBP1 fails to obtain induced DAns under physiological condition. However, the role of reactive astrocytes might be underestimated because upon brain injury, reactive astrocyte can acquire certain stem cell hallmarks that may facilitate the lineage conversion process. Therefore, whether reactive astrocytes could be genuinely converted to DAns after PTBP1 repression in a PD model needs further validation. In this study, we used Aldh1l1-CreERT2-mediated specific astrocyte-lineage-tracing method to investigate whether reactive astrocytes could be converted to DAns in a 6-hydroxydopamine (6-OHDA) mouse model of PD. However, we found that no astrocyte-originated DAn was generated after effective and persistent knockdown of astroglial PTBP1 either in the substantia nigra or in striatum, while AAV ‘leakage’ to nearby neurons was easily observed. Our results confirm that repressing PTBP1 does not convert astrocytes to DAns, regardless of physiological or PD-related pathological conditions.
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Food & Function
·
2022
·Vol. 13
·期 11
·6008-6021
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IF 6.317
Rui Ma,Qiqi Chen,Yanping Dai,Yan Huang,Qing Hou,Yuting Huang,Kai Zhong,Yina Huang,Hong Gao,Qian Bu
Ocean life contains a wealth of bioactive peptides that could be utilized in nutraceuticals and pharmaceuticals. This study aimed to obtain neuroprotective antioxidant peptides in sea squirt (Halocynthia roretzi) through protamex enzymolysis. Fraction F4 (ultrafiltration generated four fractions) had a lower molecular weight (<500 Dalton (Da)) with greater 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS) radical scavenging activities (94.24 ± 2.50% and 91.80 ± 1.19%). After gel filtration, six peptides, including Phe-Gly-Phe (FGF), Leu-Gly-Phe (LGF), Leu-Phe-VAL (LFV), Val-Phe-Leu (VFL), Trp-Leu-Pro (WLP), and Ile-Ser-Trp (ISW), were identified and sequenced by liquid chromatography-mass spectrometry (LC-MS/MS). Peptides WLP and ISW showed higher oxygen radical absorbance capacity (ORAC) values (2.72 ± 0.47 and 1.93 ± 0.01 μmol L−1 of Trolox equivalent (TE) per μmol L−1 of peptide) than glutathione (GSH). Additionally, WLP effectively increased cell viability, dramatically attenuated 6-Hydroxydopamine (6-OHDA)-induced cell apoptosis and decreased reactive oxygen species (ROS) levels to nearly two-fold, and significantly boosted glutathione peroxidase (GSH-Px) activity in PC12 cells. Transcriptome sequencing revealed differential expression of genes associated with various oxidative stress pathways after WLP treatment, such as glutathione metabolism. These results suggest that the Halocynthia roretzi-derived tripeptide WLP could alleviate neurodegenerative diseases associated with oxidative stress.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2022
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IF 5.518
Huanhuan Liu,Meng Chen,Yingnan Duan,Xiaohuan Jiang,Jiayu Liao,Mengkui Tian
In this work, macrocyclic molecule cucurbit[6]uril (CB[6])-modified two-dimensional black phosphorus nanosheets (BPNSs) used as photoactivated support were synthesized by a simple solvothermal method . Palladium nanoparticles were anchored on the surface of BP-CB[6] to obtain the composite material Pd/BP-CB[6] by using a chemical reduction method. Then, the electrocatalytic activity of the composite material modified electrode in the ethanol oxidation reaction (EOR) was investigated. The Pd/BP-CB[6] catalyst exhibits a high mass activity (1420 mA·mg Pd −1 ), which is 4.1 times higher than commercial Pd/C. Impressively, the ethanol oxidation activity of the Pd/BP-CB[6] electrode under visible light irradiation (1900 mA·mg Pd −1 ) is 1.34 times higher than under dark conditions. The result is obtained through a series of evaluations, including cyclic voltammetry , chronopotentiometry , chronoamperometry , and electrochemical impedance spectroscopy , in which the prepared Pd/BP-CB[6] shows higher catalytic activity and stability than Pd/BP. These results suggest that the synergistic effect of photocatalysis and electrocatalysis improves the electron transport kinetics of the Pd/BP-CB[6] electrode in the EOR process. Two-dimensional black phosphorus nanosheets can be used as promising photoactivated supports, to which functional material CB[6] is fixed by hydrogen bonding . Meanwhile, the electrostatic interaction between CB[6] and noble metal helps to improve the stability of the catalyst. This work provides a new idea for developing efficient photoresponsive electrode material in direct ethanol fuel cells.
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2022
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IF 4.598
Xiaoyue Liu,Hongtao Xie,Jian Mao
The porous electrodes derived from Prussian blue analogs (PBAs) show high specific capacity and superior energy densities in supercapacitors due to their tunable chemical compositions, open three-dimensional network framework and large surface area. In this work, La-Fe PBA with different morphologies is prepared at mild conditions via controlling the composition of mixed solvent and the structural evolution of the La-Fe PBA is further explored. The solubility of K 3 [Fe(CN) 6 ] and the influence of ethanol on the crystal growth is used to control the synthesis of La-Fe PBA. Four different morphologies of La-Fe PBA, such as chamfered hexagonal bipyramid microcrystals, hexagonal flakes, erythrocyte-like microstructure and small flakes, are successfully synthesized by changing the ethanol/water ratio of mixed solvent. The porous LaFeO 3 with the same shape is obtained by calcining the La-Fe PBA at 500 °C, which is subsequently employed as electrodes for battery-type supercapacitor. The erythrocyte-like LaFeO 3 shows remarkable specific capacity (330 C g −1 ) at 1 A g −1 , energy density (23.8 Wh kg −1 ) and power density (400 W kg −1 ) at 1 A g −1 . This work could provide a porous material for high-performance supercapacitors as well as an exciting strategy to develop and design materials with specific morphology.
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GLYCOCONJUGATE JOURNAL
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2022
·Vol. 39
·期 1
·131-141
·
IF 3.009
Pan Hao-Yu,Zhang Chun-Qian,Zhang Xiu-Qi,Zeng Hui,Dong Chun-hong,Chen Xia,Ding Kan
Abstract Antibiotic-associated diarrhea (AAD) is a common side-effect of antibiotic treatment resulting from an imbalance in the colonic bacteria. The hypothesis of this study is to ask whether polysaccharide from the rhizome of Dioscorea opposita which is recorded as conventional herbs and food for diarrhea treatment in Southeast Asia, may be an active compound against diarrhea induced by antibiotics. To address, firstly, a homogenous polysaccharide, DOP0.2-S-3 was characterized as a homogalacturonan containing linear repeating units of → 4)-α- D -GalA p (1 → 4)-α- D -GalA p (1 → with the average molecular weight of 14 kDa. DOP0.2-S-3 significantly reduced the water content and defecation times caused by AAD in mice, while it also remarkably attenuated the cytokines of IL-1β and IL-6 expression in mice colon tissues. DOP0.2-S-3 decreased potential pathogen and increased Bacteroidetes in the mice gut. These results suggested DOP0.2-S-3 might be a new leading compound for the functional foods or drug candidate development against AAD partially through regulating gut flora. Graphical abstract
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SCIENCE OF THE TOTAL ENVIRONMENT
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2022
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IF 10.753
Chenxi Zhao,Ruihui Xie,Qiuhui Qian,Jin Yan,Huili Wang,Xuedong Wang
As a broad-spectrum antibacterial agent, triclosan (TCS) has been confirmed to possess potential immunotoxicity to organisms, but the underlying mechanisms remains unclear. Herein, with the aid of transgenic zebrafish strains Tg ( coro 1A: EGFP) and Tg ( rag 2: DsRed), we intuitively observed acute TCS exposure caused the drastic differentiation, abnormal development and distribution of innate immune cells, as well as barriers to formation of adaptive immune T cells. These abnormalities implied occurrence of the cytokine storm, which was further evidenced by expression changes of immune-related genes, and functional biomarkers. Based on transcriptome deep sequencing, target gene prediction and dual luciferase validation, the highly conservative and up-regulated miR-19a was chosen as the research target. Under TCS exposure, miR-19a up-regulation triggered down-regulation of its target gene socs3b , and simultaneously activated the downstream IL-6/STAT3 signaling pathway. Artificial over-expression and knock-down of miR-19a was realized by microinjecting agomir and antagomir, respectively, in 1–2-cell embryos. The miR-19a up-regulation inhibited socs3b expression to activate IL-6/STAT3 pathway, and yielded abnormal changes in the functional cytokine biomarkers, along with the sharp activation of immune responses. These findings disclose the molecular mechanisms regarding TCS-induced immunotoxicity, and offer important theoretical guidance for healthy safety evaluation and disease early warning from TCS pollution.
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Materials Today Physics
·
2023
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IF 11.5
Yan Zhang,Jiayu Lu,Jin Yu,Xinbo Jiang,Yanming Wang,Xiaotian Chen,Hongbin Zhang,Liwei Yang,Yihao Yu,Dongming Qi
Modern wearable electronics are being miniaturized with high integration and frequency, leading to excess electromagnetic radiation , heat storage, and fire risk. Besides overcoming these safety issues, realizing large-scale, low-cost and recyclable preparation of multifunctional films are urgently required for practical applications of electromagnetic interference (EMI) shielding. Herein, sandwich-structured films have been prepared using commercial nylon mesh (CNM) with a waste nylon-6 silk/ammonium polyphosphate (PA6/APP) porous flame-retardant outer layer and inner conductive ultra-long silver nanowires (AgNWs) network. Only 0.2 mg/cm 2 of the conductive inner layer endows the films with excellent EMI shielding performance (53.7 dB), thermal diffusivity (5.206 ± 0.087 mm 2 /s) and antibacterial properties . In addition, the unique honeycomb structure of the PA6/APP outer layer produced during water bath treatment endows the films with superior printability. And such modified porous outer layer of the sandwich-structured films realizes a V0 rating during the UL-94 test. Even when these films are scrapped, they can still be recycled and reused. In summary, the methods described herein can be extended to realize the large-scale production of sandwich-structured films.
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BIOCATALYSIS AND BIOTRANSFORMATION
·
2023
·
IF 1.8
Zhi Chen,Zhaohe Dong,Xin Ju,Lishi Yan,Liangzhi Li,Donzhi Wei
6-Hydroxynicotinic acid emerged as a pivotal intermediate for fine chemicals. The current study aims to screen novel nicotinate dehydrogenase-producing strains. Based on the high-throughput UV coloration method, a novel nicotinate dehydrogenase-producing strain named Bacillus paramycoides was first and successfully screened from soil and gram staining as 16S rRNA gene sequencing was conducted to further confirm its accuracy. The fermentation medium formula is determined as seignette salt (5 g/L), tryptone (10 g/L), K2HPO4 (2 g/L), and nicotinic acid (4 g/L) after a single factor and orthogonal test. The activity of nicotinate dehydrogenase produced by B. paramycoides after optimization reached 1.23 U/mL, which was the highest ever reported. In addition, the enzyme characterization results revealed that the optimum reaction temperature and pH were 40 °C and 7.0, respectively. Furthermore, B. paramycoides nicotinate dehydrogenase exhibited good thermostability with more than 50% of the highest enzyme activity retained at 50 °C after 8 h incubation, which was better than ever reported. The pH stability results suggested that nicotinate dehydrogenase possessed a narrow pH ranging from 6.0 to 8.0. In addition, shaker speed demonstrated a positive relation with enzyme activity since shaker speed could affect mass transfer and dissolved oxygen concentration. Finally, it could completely transform nicotinic acid to 6-HNA within 3 h when the substrate loading is 15 g/L. In conclusion, these results indicated that B. paramycoides nicotinate dehydrogenase could serve as a potential candidate for bioproduction of 6-HNA.
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ENVIRONMENTAL RESEARCH
·
2023
·
IF 8.3
Jiaohui Xia,Yan Li,Xinbai Jiang,Dan Chen,Jinyou Shen
Zero-valent iron based autotrophic denitrification (ZVI-AD) has attracted increasing attentions in nitrate removal due to saving organic carbon budget in wastewater treatment , but limited by the low reaction speed, poor electron transfer efficiency as well as the compaction/blocking by iron hydrolysis products. Humic substances (HS) were promising to regulate iron cycle and accelerate electron transfer by serving as electron mediators. In this study, HS analogue, antraquinone-2, 6-disulfonate (AQDS), was added to enhance ZVI-AD process. Results showed that the dosage of AQDS led to a NO 3 − -N removal efficiency of 83.37 ± 3.98% within 96 h, which was 32.28 ± 1.25% higher than that in ZVI-AD system. The corrosion of ZVI and microbially nitrate reduction were both improved at the presence of AQDS. The addition of AQDS enriched the functional species, including autotrophic denitrobacteria namely Thauera and Hydrogenophaga , iron redox-related species namely Ferruginibacter and HS respiration related species namely Flavobacterium . The genes napA and napB related to electron transfer, nirK and nosZ related to the accumulation of intermediate products were also enriched by the addition of AQDS. AQDS addition boosted the electrons flowing to both abiotic and biotic nitrate reduction. Nitrate removal mechanism involved in ZVI-AQDS coupled system was proposed. This study provided an alternative strategy for improving ZVI-AD by HS.
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POLYMER TESTING
·
2023
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IF 5.1
Yufang Li,Danli Nie,Ziqing Cai
In this paper, the mechanical properties, thermal properties, oil resistance and tribology performance of hydrogenated nitrile/polyamide 6 blends were studied systematically. The inter-molecular interaction, phase separation structure, crystallinity, dynamic loss, friction morphology and other aspects of the blends were investigated. The results showed that PA6 phase had significant reinforcing effect in HNBR matrix. Due to the strong interaction between PA6 molecular chain and HNBR molecular chain, the glass transition temperature (T g ) of the blends was increased and the dynamic loss factor (tanδ) was decreased. Meanwhile, the tribology performance of the blends was significantly improved. It was due to the formation of a microball layer with PA6 particle phase as component, which effectively isolated the contact between the friction pairs.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Muhammad Muhammad,Chang-sheng Shao,Qing Huang
A label-free, highly sensitive, and selective biosensor for detection of interleukin-6 (IL-6), which is an important inflammatory and cancer progression cytokine, has been achieved by combining gold nanoparticles (NPs) array surface-enhanced Raman spectroscopy (SERS) substrate and DNA aptamer. The SERS substrate consisted of Au NPs array is functionalized with the aptamer which is composed of the IL-6 recognition sequence and the output signal reporter. Upon recognition of target IL-6 in serum, the aptamer changes its conformation, resulting in the corresponding change of the output Raman intensity ratio (I 660 /I 736 ), with which the quantitative evaluation of IL-6 in 10 -12 -10 -7 M range can be realized. This SERS approach was employed to detect IL-6 in mice serum for inspecting the effect of drug and bacterial infection, and particularly, the radiation injury in the treated animals. This work therefore exemplifies that the aptamer-based SERS biosensor can serve as a promising tool for quick and convenient medical diagnosis applications.
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POLYMER INTERNATIONAL
·
2021
·Vol. 70
·期 9
·1376-1385
·
IF 2.99
Yanli Li,YeFang Yang,Xinqi Pei,Yu Li,Yuhui Yuan,Xiaoling Huang
Cucurbit[6]uril-derivatized vinyl monomer (cucurbit[6]uril methacrylate) was synthesized. Acrylic acid (AA) was used as the comonomer for copolymerization with the cucurbit[6]uril-derivatized monomer. A series of copolymers of poly(acrylic acid- co -cucurbit[6]uril methacrylate) (PAA- co -CB[6]MA) were synthesized by free radical polymerization. The cucurbit[6]uril-derivatized monomer and PAA- co -CB[6]MA copolymers were characterized by 1 H NMR spectra, high-resolution mass spectroscopy and gel permeation chromatography. The solution behavior was examined using UV–visible spectroscopy and dynamic light scattering. The PAA- co -CB[6]MA showed an upper critical solution temperature (UCST) in water. The UCST-type thermoresponsive behavior of these CB[6]-derivatized copolymers was highly dependent on the composition of the copolymer. For the same type of copolymer, changes in its composition gave rise to different UCSTs. Additionally, the PAA- co -CB[6]MA copolymers were successful in trapping azocarmine B. The stimuli-responsive release of encapsulated azocarmine B was achieved by a combination of temperature and pH value stimuli. According to the results, the novel PAA- co -CB[6]MA copolymers can be used as carriers. © 2021 Society of Industrial Chemistry.
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