ACS Omega
·
2024
·
IF 3.7
Hechen Sun,Lening Zhang,Nan Zhao,Hua Xin
Lung cancer poses a significant threat to human health. Surgical intervention is the preferred treatment modality for lung cancer, but a large number of patients are deprived of the opportunity for surgery for various reasons and are compelled to undergo radiotherapy and chemotherapy, which entail systemic adverse reactions. In recent years, with the advancement of nanomedicine, chemodynamic therapy (CDT) based on free radicals has been extensively investigated. In this study, we fabricated copper–citrate–chitosan composite nanoparticles (CuCC NPs) by encapsulating copper–citrate complexes with natural chitosan polymers, resulting in a substantial reduction in the biotoxicity of copper ions. The CuCC NPs selectively accumulated in tumor tissues through the enhanced permeability and retention effect (EPR) and gradually degraded within the acidic and glutathione (GSH)-rich microenvironment of the tumor, thereby releasing the loaded copper ions. Through CDT, the copper ions converted the overexpressed hydrogen peroxide (H2O2) in the tumor tissue into hydroxyl radicals (•OH), leading to the eradication of tumor cells. In animal experiments, CuCC NPs exhibited remarkable efficacy in CDT. Further histopathological and hematological analyses demonstrated that CuCC NPs could induce substantial apoptosis in tumor tissues while maintaining an extremely high level of safety.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Today Chemistry
·
2024
·
IF 6.7
Junyu Chen,Yanqin Ma,Xinjian Cheng,Yufan Wu,Huizhi Chen,Cheng Liu
Natural polymer chitosan has many advantages, but the poor solubility limits its application. In this study, chitosan derivatives soluble in DCM, DMF, DMSO, H 2 O and other solvents were developed by introducing side chains into chitosan (CS). In addition, the fluorescent group on the side chain makes the chitosan derivative have the ability to detect 4-nitrophenol (4-NP). Firstly, a chain transfer agent containing C C (N3-NS) was synthesized with naphthalimide as the fluorescence unit. Then, using AIBN as initiator, an extensively soluble polymer with fluorescent moiety TP-NVP was synthesized by reversible addition-fragmentation chain transfer polymerization (RAFT) method using monomer N -vinyl pyrrolidone (NVP). Finally, the C C of TP-NVP was used to react with –NH 2 on CS to prepare PVP-g-chitosan, so that more CS was “pulled” by TP-NVP into the solvent. The solubility of the synthesized CS-PVP probe can be adjusted by adjusting the amount of NVP, up to 17.6 mg/mL. At the same time, the synthesized CS-PVP had a good detection effect on 4-NP, and the detection limit (LOD) was 0.054 μM. The HOMO-LUMO energy level of the probe was calculated by cyclic voltammetry (CV). In addition, CS-PVP was successfully loaded on a low background fluorescent test paper to achieve real-time visual intelligent determination of 4-NP in real samples with the help of a color scanning APP of a smartphone.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2024
·
IF 9.4
Shuai Ma,Li Zhang,Yang Wu,Wei Huang,Fangtian Liu,Mingguang Li,Yifeng Fan,Haibin Xia,Xianguo Wang,Xinzhi Li,Hongbing Deng
Cartilage is severely limited in self-repair after damage, and tissue engineering scaffold transplantation is considered the most promising strategy for cartilage regeneration. However, scaffolds without cells and growth factors, which can effectively avoid long cell culture times, high risk of infection, and susceptibility to contamination, remain scarce. Hence, we developed a cell- and growth factor-dual free hierarchically structured nanofibrous sponge to mimic the extracellular matrix, in which the encapsulated core–shell nanofibers served both as mechanical supports and as long-lasting carriers for bioactive biomass molecules (glucosamine sulfate). Under the protection of the nanofibers in this designed sponge, glucosamine sulfate could be released continuously for at least 30 days, which significantly accelerated the repair of cartilage tissue in a rat cartilage defect model. Moreover, the nanofibrous sponge based on carboxymethyl chitosan as the framework could effectively fill irregular cartilage defects, adapt to the dynamic changes during cartilage movement, and maintain almost 100 % elasticity even after multiple compression cycles. This strategy, which combines fiber freeze-shaping technology with a controlled-release method for encapsulating bioactivity, allows for the assembly of porous bionic scaffolds with hierarchical nanofiber structure, providing a novel and safe approach to tissue repair.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Linlin Wang,Lilong Wang,Nan Wang,Chen Song,Chengrong Wen,Chunhong Yan,Shuang Song
Chitosan (CTS) and chitosan oligosaccharides (COS) have been widely applied in food industry due to their bioactivities and functions. However, CTS and COS with positive charges could interact with proteins, such as whey protein isolate (WPI), influencing their digestion. Interaction among CTS/COS, FUC, and WPI/enzymes was studied by spectroscopy, chromatography, and chemical methods in order to reveal the role of FUC in relieving the inhibition of protein digestibility by CTS/COS and demonstrate the action mechanisms. As shown by the results, the addition of FUC increased degree of hydrolysis (DH) and free protein in the mixture of CTS and WPI to 3.1-fold and 1.8-fold, respectively, while raise DH value and free protein in the mixture of COS and WPI to 6.7-fold and 1.2-fold, respectively. The interaction between amino, carboxyl, sulfate, and hydroxyl groups from carbohydrates and protein could be observed, and notably, FUC could interact with CTS/COS preferentially to prevent CTS/COS from combining with WPI. In addition, the addition of FUC could also relieve the combination of CTS to trypsin, increasing the fluorescence intensity and concentration of trypsin by 83.3 % and 4.8 %, respectively. Thus, the present study demonstrated that FUC could alleviate the inhibitory effect of CTS/COS on protein digestion.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Polymers
·
2024
·Vol. 16
·期 11
·1555
·
IF 4.7
Sheng Yan,Yunren Qiu
The most prevalent type of hemodialysis membrane is polysulfone (PSf). However, due to inadequate biocompatibility, it significantly compromises the safety of dialysis for patients. In this study, we modify the surface of the PSf membrane with 2,4-dihydroxybenzophenone (DBPh) groups to serve as anchoring sites during UV irradiation. Subsequently, a tailored sulfonated dihydroxy propyl chitosan (SDHPCS) is grafted onto the modified PSf membrane to compensate for the deficiencies in hydrophilic additives. The modified PSf membrane exhibits outstanding hydrophilicity and stability, as demonstrated by its characterization and evaluation. This paper focuses on investigating the interaction between platelet membrane formation, protein adsorption, and anticoagulant activity. The results show that the modified PSf membrane exhibits remarkable enhancement in surface hydrophilicity, leading to a significant reduction in protein and platelet adsorption as well as adhesion.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Hongyi Ren,Shengzi Li,Keying Ding,Yan Wang,Qiping Zhan,Yonghua Zheng,Zhengguo Wu,Peng Jin
After harvest, fruits and vegetables go through the stages of storage, transportation and sales, and how to regulate the role of preservative in different stages is an urgent problem to be solved. Herein, chitosan-stearic acid is synthesized, then interacting with trans-2-hexenal (E2H) to prepare nanoclusters. Subsequently, the temperature-sensitive liposomes were designed to encase E2H by using the special temperature-responsive open-chain phospholipids, and then combined with chitosan to obtain chitosan-based coating film for peach preservation. The thermosensitive liposomes were uniformly distributed, highly dispersed and spherical, which has excellent temperature response characteristics, and the release of active substances could be regulated in the temperature range of 10 °C-40 °C to achieve the effect of on-demand release. In addition, thermosensitive liposomes also have excellent antioxidant and antibacterial activities. Therefore, the obtained chitosan-based coating could maintain the freshness of peaches, improve the quality and extend the storage period of peaches. The edible chitosan coating and active preservative prepared in this study have potential application value in responsive intelligent active packaging.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yongyi Yang,Rong Liu,Li Xie,Yang Li,Mengqi Chen,Huang Zhu,Qingqing Pan
Injectable hydrogels can be suitable for any irregular wounds to play an important role in wound healing, which have attracted much attention from researchers. Here, a chitosan-based double alternating injectable hydrogel had been proposed, which aligned with characteristics of wound healing. First of all, carboxymethyl chitosan (CMCS) and oxidized gellant (OGG) were used to fabricate above double-alternating injectable hydrogel skeleton (COG) through Schiff base bond. Then, dipotassium glycyrrhizinate (DPG) and bioactive glass (BG) were loaded respectively into COG, forming COG2-D10 and COG2-B0.5 hydrogel. Various properties of above hydrogels such as rheological behaviors, injectable capability, cytocompatibility, antibacterial and anti-inflammatory activity were systematically investigated. The results indicated that above hydrogel not only had a stable network structure, good toughness, injectability, and excellent adhesive properties, but also exhibited efficient antibacterial, anti-inflammatory, hemostatic, and biocompatible properties. The investigation of in vivo full-thickness skin wound healing showed that compared with COG2-D10 or COG2-B0.5 hydrogel alone, the double-alternating COG2-D10/COG2-B0.5 hydrogel could better enhance the efficacy of drugs, promote cell migration and proliferation, accelerate collagen fiber deposition and neovascularization, and significantly improve wound healing ( p < 0.01). Therefore, the double-alternating therapy strategy of hydrogel will provide a new approach for the clinical treatment of skin wound healing.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Zhenzhen Hui,Xinyuan Pan,Ying Li,Chensong Zhang,Xuzhong Zuo,Jing Tang,Yanping Wang,Nannan Qiu,Shengbiao Zheng,Xiangju Ye,Ruizhang Hu,Dongpo Song,Wei Fang,Jie Yang,Guoqing Yan
Dynamic antibacterial polysaccharide prodrug hydrogels are in great demand for treatment of wound infection owing to their unique advantages such as excellent biocompatibility, superior antimicrobial property as well as favorable wound healing capacity. Herein, this work highlights the successful development of a dynamic carboxymethyl chitosan (CMC) prodrug hydrogel, which is facilely constructed through Schiffer base reaction between antibacterial components (amikacin and CMC) and crosslinker (dialdehyde PEG). Moderate dynamic imine linkages endow the hydrogel with excellent injectable and self-healing capability as well as targeted on-demand drug release in slightly alkaline condition at infected wound. All ingredients and their strong intermolecular interactions endow the hydrogel with favorable swelling and moisture retention capability. Moreover, the covalent and non-covalent interactions also endow the hydrogel with superior adhesion and mechanical property. These attractive characteristics enable hydrogel to effectively kill pathogens, promote wound healing and reduce side effects of amikacin . Thereby, such a dynamic CMC prodrug hydrogel may open a new avenue for a robust therapy on wound infection, greatly advancing their use in clinics.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2025
·
IF 12.5
Zhuo Xu,Bin Wu,Chunhua Wu,Qian Chen,Yanfei Niu,Zhengjun Shi,Kun Liang,Xiaoping Rao
In order to the antifungal activity of chitosan (CS) and to obtain a better natural bio-antimicrobial agent, CS was modified with acrylpimaric acid (APA). The grafting sites of APA on CS were controlled by adjusting the reaction time and the ratio of reactants to obtain APA grafted with CS C 2 -NH 2 (NCSAA) and C 6 -OH (CSAA). Intermediates to protect C 2 -NH 2 (PMCSAA) and final sample derivatives (PCSAA) were prepared using phthalic anhydride. The structural and crystal variations of the four derivatives were analyzed and determined by FTIR, 1 H NMR and XRD. The in vitro antifungal activities of the four modified samples against V. mali, C. lunata, A. solani, F. oxysporum f. sp. niveum, and F. graminearum were evaluated by the mycelial growth rate method, and PCSAA was screened for optimal inhibitory effect on V. mali with an EC 50 = 38.66 μg/mL. The SEM and TEM showed that these derivatives cause the loss of mycelial contents by disrupting the ultrastructure of the mycelium, destroying the internal structure of the mycelium, and preventing the cells from carrying out their normal life activities. In vivo experiments showed better curative effect (79.09 % ± 2.00) and protective effect (80.93 % ± 0.03) of PCSAA at 200 μg/mL.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2025
·
IF 12.4
Ran Li,Zeqi Liu,Juan Guo,Wenbo Xu,Xuan Ning,Xiaojun Li,Meng He,Qingye Liu
s To address the vulnerability of fruit during the storage period, an economical and green active film package is urgently required to extend the shelf life. In this work, a vanillin-derived dialdehyde (DV) was successfully synthesized, and further employed as the crosslink agent into chitosan matrix via the formation of Schiff base. The obtained film (CS-DV) with compact structure exhibited excellent stability, mechanical strength, barrier properties and bioactivities. The experimental results indicated that the tensile fracture stress was enhanced by 3.8 ∼ 4.5 times in comparison with pure chitosan (CS) film. Due to the contained DV molecules, the UV irradiation in range of 250 ∼ 400 nm was almost blocked by CS-DV films. Meanwhile, the diffuse of oxygen/water vapor across the CS-DV film were suppressed, preventing the dehydration and nutrient oxidation. Moreover, CS-DV films displayed high radical scavenging capacities, where the highest DPPH and ABTS radical scavenging ratios were evaluated to be 82.20% and 99.39%, respectively. In combination with their strong antibacterial activities against Staphylococcus aureus and Escherichia coli , CS-DV film package could significantly extend the shelf life of raspberry fruits with high preservation efficiency. This bio-based film only consists chitosan and vanillin derivative without any additives, showing potential as packaging material for agricultural and food products.
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2024
·Vol. 48
·期 22
·10019-10025
·
IF 2.7
Yaoge Huang,Wuga Shama
This study prepared epoxy resin reinforced porous beads (CERBs) by crosslinking a water-soluble epoxy monomer, 1,4-butanediol diglycidyl ether (BDE), with chitosan (CS) using isophorone diamine (IPD) as a crosslinking agent. CERBs are prepared by a one-step method under atmospheric pressure and used for the adsorption of Congo Red (CR). The Sips isotherm model indicates that the maximum adsorption capacity of CERBs for Congo Red is 2289.2 mg g−1. CERBs have a three-dimensional network structure and strong mechanical properties, and can be reused more than 5 times maintaining a high adsorption capacity. Therefore, this material can be produced on a large scale and has great potential for commercial applications.
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2011
·
IF 3.463
Jiliang Wu,Chunyan Liao,Jun Zhang,Wenze Cheng,Nuo Zhou,Sheng Wang,Ying Wan
Chitosan microspheres loaded with transforming growth factor-β1 (TGF-β1) were first prepared with an emulsification method using genipin as crosslinker, and the selected microspheres were then embedded into porous scaffolds built by chitosan-polycaprolactone with polycaprolactone content of around 42 wt.%. Some optimized chitosan-polycaprolactone scaffolds with porosity higher than 80% and having an initial TGF-β1 load of around 3 ng(TGF-β1)/mg(dry scaffold) were capable of maintaining sustained release of TGF-β1 in a simulant in vivo environment at controlled rates over a period of time longer than four weeks without severely initial burst. The chitosan-polycaprolactone scaffolds also showed well-defined compressive properties in wet state with compressive stress at 10% strain and modulus higher than 100 kPa and 1000 kPa, respectively, which are almost ten-fold higher than that of corresponding microsphere-embedded chitosan scaffolds. The obtained results suggest that potential applications of the scaffolds in articular cartilage repair.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）