JOURNAL OF POLYMER RESEARCH
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2017
·Vol. 24
·期 4
·1-7
·
IF 1.615
Li Hu,Zhou Zhen,Liu Jingcheng,Zheng Xiangfei,Xu Wenjia,Ji Changwei,Shi Weidong,Liu Ren,Liu Xiaoya
Hexakis(4-formylphenoxy) cyclotriphosphazene (HAPCP), substitution reaction product of hexachlorotriphosphazene (HCTP) with p-hydroxybenzaldehyde, was reduced by sodium borohydride to afford hexakis[4-(hydroxymethyl) phenoxyl] cyclotriphosphazene (HHPCP). The hydroxyl group of this compound was then protected by the reaction with di-tert-butyl dicarbonate ester (DBDC) in the presence of 4-dimethylaminopyridine (DMAP) as catalyst to give t-butyloxy carbonyl (tBOC) protected HHPCP. Fourier-transform infrared spectroscopy (FT-IR) and proton nuclear magnetic resonance ( 1 H NMR) were employed to characterize the chemical structure, indicating that the synthesis was successful. Thermal behavior was characterized by thermogravimetric analysis (TGA), the results showed that the initial thermal decomposition temperature occurred at approximately 170 °C, X-ray diffraction analysis (XRD) results showed that the tBOC protected HHPCP derivatives had amorphous form, and performed excellent film-forming property. Upon irradiation to 365 nm exposure, the photoacid generated proton H + , which can catalyze the deprotection of acid labile tBOC group. This type of novel molecular glass photoresist based on hexachlorotriphosphazene showed high sensitivity, making it possible to fabricate 1 μm line and space pattern under i-line exposure.
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POLYHEDRON
·
2017
·
IF 1.926
Ling-Dong Chen,Lin-Xia Zhou,Yue-Qing Zheng,Hong-Lin Zhu
Two new supramolecular complexes [Ag 2 (MA) 2 (2-nbd)]·2.5H 2 O ( 1 ) and [Ag 2 (MA) 2 (3-npa)]·2H 2 O ( 2 ) (2-H 2 nbd = 2-nitroterephthalic acid; MA = melamine; 3-H 2 npa = 3-nitrophthalic acid) have been synthesized under ultrasound conditions and characterized by infrared spectra (IR), thermogravimetric analysis (TGA), elemental analyses (EA) and X-ray diffraction (XRD). Complex 1 exhibits 2D framework through the bridging of carboxylic acid ligand and melamine ligand. Complex 2 presents a binuclear silver structural unit, which was further construct to three-dimension structure via hydrogen-bond interaction. The photocatalytic performance and photoluminescent properties of them were also investigated. And the results show that complex 1 and 2 exhibited good photocatalytic activity towards the degradation of Rhodamine B (RhB) and Methylene Blue (MB) under visible light irradiation. Moreover, it is verified that the degradation rate fits the first-order kinetics.
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JOURNAL OF CELLULAR PHYSIOLOGY
·
2017
·Vol. 233
·期 6
·4919-4925
·
IF 4.08
Ji Li,Mincheng Zhang,Yong Mao,Yimao Li,Xiaoxia Zhang,Xuehan Peng,Feng Yu
Aristolochic acid I (AA-I), one of the main active components in Aristolochaia herbs, may induce aristolochic acid nephropathy (AAN). Renal interstitial fibrosis is one of the most typical features of AAN. To investigate the mechanism of Aristolochic acid I (AA-I) −induced renal epithelial-mesenchymal transition (EMT) and determine the role of aquaporin-1 (AQP1) in this process, we established an AA-I-induced EMT model in human proximal tubular epithelial cells (HK-2 cells). Morphological examination, MTT assay, and Western blot analysis were performed. Aquaporin 1 (AQP1) and several EMT-related proteins were detected, thereby suggesting the occurrence of AA-I-induced EMT. Two main pathways of transforming growth factor-β (TGF-β) signaling, namely, Smad-dependent and Smad-independent signaling pathways, were also detected. The results showed that the TGF-β / Smad-independent signaling pathways (β-catenin, Ras-Raf-Erk1/2 signaling pathways) were activated, and AQP1 expression was decreased during the AA-I induced EMT on HK-2 cells. With the presence of TGF-β1 receptor inhibitor (LY364947) and Erk1/2 inhibitor (PD98059), AQP1 expression was altered by PD98059, suggested that AQP1 could be adjusted by Erk1/2 signaling. Moreover, the inhibitory effect of AA-I on AQP1 was stronger than that of TGF-β1, suggested that AQP1 may be an important target on AAN clinical therapy.
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INORGANIC CHEMISTRY
·
2013
·
IF 4.593
Yu-Peng Zhou,En-Bao Liu,Jin Wang,Hsiu-Yi Chao
Newly prepared gold(I) acetylide-based luminescent cation sensors 1c and 1d, which bear a novel three–armed flexible conformation, together with control molecule 2c, were synthesized in four steps. 1c and 1d exhibit the π → π* absorption of the acetylide ligands in fluid solutions and produce the 3(ππ*) emission of the acetylide ligands in the solid state and degassed THF solution. The 3(ππ*) emission of 1c in DMSO can be turned on upon addition of Ag+. The binding ratio between 1c and Ag+ as well as the binding constant log K were determined as 1:1 and 4.35 ± 0.12 respectively by UV–vis titration experiments. The formation of [1c·Ag]+ adduct, which leads to the appearance of new up-field peaks, was confirmed by 1H NMR spectroscopic titrations. The control 1H NMR titration experiments for the acetylide-free analogue 1a and single-armed analogue 2c indicate the acetylide groups and tripodal structures are responsible for the binding of Ag+. The control experiment for tripodal gold(I) acetylide analogue 1d suggests the change of PPh3 with P(2-Py)Ph2 induces similar binding affinity toward Ag+ but less selectivity toward Ag+. Free 1c also exhibits the anion binding affinity toward F–, but the Ag+ adduct [1c·Ag]+ shows less affinity toward F–.
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MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Yuanyi Wu,Yan Yang,Shihao Yang,Yang Ju,Yang Jiang,Yumei Xiao,Jialin He,Tian Liu,Leyao Tang,Kaiyong Wang,Yi Yang
Interfacial and interlayer interactions between layered double hydroxides (LDHs) and nucleic acids offer LDHs great potential to absorb and detect nucleic acids. Herein, we demonstrate a simple, rapid, and versatile fluorescent sensing platform based on two-dimensional LDHs and exonuclease I (Exo I) for the selective analysis of DNA and microRNA. ZnNiAl-LDHs were utilized as quenchers for FAM labelled on single-stranded DNA (ssDNA) probes via dynamic quenching with high fluorescence quenching ability. The quenching mechanism was explored by means of spectral analysis, characterization experiments, DNA adsorption and desorption studies, zeta potential analysis, and molecular dynamics simulations. The driving forces for the adsorption of LDHs toward fluorophore-labelled nucleic acids were confirmed to be electrostatic interaction, van der Waals force, and anion exchange for the first time. In order to distinguish the formed target/probe duplexes from FAM-ssDNA probes, Exo I was introduced to specifically hydrolyze FAM-ssDNA probes. The fabricated fluorescent sensing platform for nucleic acids detection shows a low detection limit of 0.08 nM. Finally, this sensor was effectively utilized for the detection of DNA and microRNA in actual biological and environmental samples, achieving recoveries ranging from 96.4 % to 105 %, indicating high accuracy. This work proposed a promising sensing platform for sequence-specific nucleic acids detection, and provides a new perspective on two-dimensional LDHs as structural supports and biomolecular reservoirs for biomedical applications.
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JOURNAL OF FLUORESCENCE
·
2024
·
IF 2.6
Li Ling,Li Zheng-Ling,Wang Wei,Long Xin-Xin,Liu Ping,Wang Guo-Tian,Sun Shi-Qing,Du Rong-Lian
The rapid and accurate detection of programmed death-ligand 1 (PD-L1) expression is of great value in the diagnosis and treatment of tumors. ELISA-based traditional method is the gold standard for protein detection, but there are still some shortcomings, especially the antigen-antibody dependence, greatly increased the detection time and cost. This work constructed a label-free fluorescent probe for rapid and sensitive detection of PD-L1 using a truncated aptamer as recognition molecules and double-stranded DNA specific dyes (SYBR Green I) as signal units. After a series of optimization conditions, this probe has good detection capability for PD-L1 in buffer solution with the detection limit as low as 0.68 ng/mL. Due to the specific recognition ability of aptamer and target, this method also has good selectivity for PD-L1 detection. The recovery of PD-L1 in human serum samples ranges from 86.20 to 96.36%. Compared with other methods, this strategy does not need to be marked, and does not need other complex design and purification process, but simple operation process and strong anti-interference ability. The whole detection process can be completed within 20 min and has good application prospect. This work will provide reference for drug dosage and prognosis evaluation of specific tumor therapy.
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COMPOSITES COMMUNICATIONS
·
2024
·
IF 6.5
Hui Ke,Jiamei Lai,Yilong Li,Xiaomei Xue,Xiang Liu,Jin Cao
Carbon fiber-reinforced polymer (CFRP) composites are commonly used in structural components because of their favorable mechanical properties. However, their limited interlayer toughness, resulting from their laminated structure and inherent brittleness, restricts their broader range of applications. The study's goal was to make the matrix system stronger by connecting carboxylated multi-walled carbon nanotubes (MWCNTs-C) with polydopamine (PDA) and polyethyleneimine (PEI). A ternary resin system consisting of epoxy resin (EP) and vinyl ester resin (VER) was created. This system has an interpenetrating network (IPN)structure and modified particles, which improves the interlayer toughness of unidirectional CFRP laminates. The modified MWCNTs-C has better surface reactivity, which lets it spread out well inside the IPN structure made by EP and VER. This reduces the "self-filtering effect" of the particles that come into contact with the VARTM process. The incorporation of 1 wt% of modified MWCNTs-C particles leads to a significant 107% improvement in the Mode I interlaminar fracture toughness of unidirectional CFRP composites with G ⅠC ,prop value. This suggests that 1 wt% is the optimal amount of particle inclusion. The Mode I interlaminar fracture toughness (G ⅠC ,prop) of unidirectional CFRP composites goes up by 124% when IPN structural toughening is added, reaching a value of 1216 J/m 2 . They found that adding IPN structural toughening to unidirectional CFRP composites raised the Mode I interlaminar fracture toughness (G ⅠC ,prop) by 124%, giving it a value of 1216 J/m 2 . We improved the Mode I interlaminar fracture toughness (G ⅠC ,prop) of unidirectional carbon fiber reinforced polymer (CFRP) composites by 147% by adding particle reinforcement and interpenetrating polymer network (IPN) architecture. The highest value recorded was 1340 J/m 2. Concurrently, the synergistic toughening process was examined by comparing empirical data and scanning electron microscope pictures. This analysis offers a valuable point of reference for choosing the interlaminar toughening method for carbon fiber-reinforced polymer (CFRP) composites.
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INORGANIC CHEMISTRY
·
2024
·
IF 4.3
Jiahui Zhao,Yixuan Zhao,Xuefeng Shao,Ying Sun,Huang Wen,Jian Liu
Developing a synthesis-free, multifunctional, and effective colorimetric assay system based on 3,3′,5,5′-tetramethylbenzidine (TMB) oxidation is attractive yet challenging. Herein, we established a synergetic Cu2+/Cr6+-promoting strategy for TMB-based colorimetric detection of Cr6+. By introducing Cu2+, critical TMB·+···Cu(I/II)···TMB charge transfer complex (TMC) intermediates were in situ formed to reduce the activation energy of TMB oxidation, thereby accelerating Cr6+-mediated TMB oxidation. TMC intermediates also played a pivotal role in H2O2-participated TMB oxidation, clarifying the secondary responsibility of reactive oxygen species frequently caused by Fenton-like reactions. Leveraging the synergetic capacity between Cu2+ and Cr6+ for TMB oxidation, we demonstrated sensitive and specific colorimetric detections for Cr6+ with a limit of detection of 0.006 μM. With its convenient operation and rapid responsiveness, this strategy successfully enabled the practical detection of Cr6+ in real water samples. This work not only enhances the understanding of the internal acceleration mechanism in colorimetric sensing but also provides valuable opportunities to advance synthesis-free detection platforms.
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Analytical Methods
·
2024
·Vol. 16
·期 39
·6715-6725
·
IF 2.7
Shenglan Zhang,Liqiang Chen,YuXin Tan,Shaojie Wu,Pengxin Guo,Xincheng Jiang,Hongcheng Pan
The rising demand for point-of-care testing (POCT) in disease diagnosis has made LFIA sensors based on dendritic metal thin film (HD-nanometal) and background fluorescence technology essential for rapid and accurate disease marker detection, thanks to their integrated design, high sensitivity, and cost-effectiveness. However, their unique 3D nanostructures cause significant fluorescence variation, challenging traditional image processing methods in segmenting weak fluorescence regions. This paper develops a deep learning method to efficiently segment target regions in HD-nanometal LFIA sensor images, improving quantitative detection accuracy. We propose an improved UNet++ network with attention and residual modules, accurately segmenting varying fluorescence intensities, especially weak ones. We evaluated the method using IoU and Dice coefficients, comparing it with UNet, Deeplabv3, and UNet++. We used an HD-nanoCu-Ni LFIA sensor for cardiac troponin I (cTnI) as a case study to validate the method's practicality. The proposed method achieved a 96.3% IoU, outperforming other networks. The R2 between characteristic quantity and cTnI concentration reached 0.994, confirming the method's accuracy and reliability. This enhances POCT accuracy and provides a reference for future fluorescence immunochromatography expansion.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Mingyue Ye,Xianfeng Lin,Jin Li,Xiaowan Chen,Dichen Ying,Shijia Wu,Zhouping Wang,Nuo Duan
Malachite green (MG) has been illicitly employed in aquaculture as a parasiticide, however, its teratogenic and carcinogenic effects pose a significant human health threat. Herein, a dual-mode colorimetric and electrochemical aptasensor was fabricated for MG detection, capitalizing on the robust catalytic and peroxidase-like activity of P-CeO 2 NR@Mxene and good capture efficiency of a tetrahedral DNA nanostructure (TDN) designed with multiple aptamers (m-TDN). P-CeO 2 NR@Mxene-modified complementary DNA (cDNA) served as both colorimetric and electrochemical probe. m-TDN was attached to AuE to capture MG and P-CeO 2 NR@Mxene/cDNA. The superior aptamer and MG binding to cDNA regulated signals and enabled precise MG quantification. The further introduced Exo I enabled aptamer hydrolysis, releasing MG for further binding rounds, allowing target recycling amplification. Under the optimal conditions, the aptasensor reached an impressively low detection limit 95.4 pM in colorimetric mode and 83.6 fM in electrochemical mode. We believe this dual-mode approach holds promise for veterinary drug residue detection.
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ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Yifan Cui,Dezhi Yang,Qiulan Li,Zhongmei Peng,Zitao Zhong,Yuzhu Song,Qinqin Han,Yaling Yang
Nanozyme-mediated antioxidative therapy is a promising star for treating a myriad of important diseases through eliminating excessive reactive oxygen species (ROS) such as O2·– and H2O2, a critical mechanism for inflammatory bowel disease (IBD). This work provides a high biocompatibility iodine–copper–zinc covalent doped carbon dots (Cu,Zn,I-CDs) with the catalase (CAT)-, superoxide dismutase (SOD)- and glutathione peroxidase (GPx)-like catalytic activities for treating ulcerative colitis (UC) by scavenging overproduced ROS. We found that I dopant aids in counteracting the positive charge at Cu,Zn dopants brought on by low pH, enabling Cu,Zn,I-CDs to process strong triple antioxidant nanozyme activities rather than Cu,Zn-CDs. Vitro experiments displayed that the Cu,Zn,I-CDs could scavenge the excessive ROS to protect cellular against oxidative stress and reduce the expression of proinflammatory cytokines, such as TNF-α, IL-1β, and IL-6. In sodium dextran sulfate (DSS)-induced colitis mice models, Cu,Zn,I-CDs with excellent biocompatibility could effectively relieve the inflammation of the colon, containing the reduction of the colon length, the damaged epithelium, the infiltration of inflammatory cells, and upregulation of antioxidant genes. Therefore, the therapy of Cu,Zn,I-CD antioxidant nanozymes is an effective approach and provides a novel strategy for UC treatment.
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JOURNAL OF MOLECULAR STRUCTURE
·
2025
·
IF 4.7
Ya-Kun Zhang,Jian-Bo Tong,Hong-Yan Zhang,Mu-Xuan Luo,Zhi-Peng Qing,Cheng-Jian Tan
Topoisomerase I (Top I) plays a pivotal role in resolving DNA supercoiling during replication and transcription, highlighting its significance as a therapeutic target in oncology. Despite the clinical efficacy of inhibitors such as Irinotecan and Topotecan, their clinical utility is hindered by the emergence of resistance mechanisms. Sophoridine, a natural alkaloid, has demonstrated promising Top I inhibitory activity, with its derivatives showing enhanced antitumor effects. In this study, the Topomer CoMFA approach was utilized to construct a validated 3D-QSAR model to explore the structure-activity relationships (SAR) of sophoridine derivatives. Based on the model's guidance, nine derivatives were designed, four of which were synthesized and subjected to biological evaluation. Computational investigations, including molecular docking, molecular dynamics simulations, and ADMET predictions, provided detailed insights into their binding interactions and drug-like properties. These findings establish a robust theoretical framework for the rational design of sophoridine-based Top I inhibitors with potential applications in cancer treatment.
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