INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Ning Zhang,Lina Yang,Huaying Pan,Siying Xing,Jinxin Li,Hong Song,Dayu Zhou,Chunfang Ma,He Liu
Conventional plastic packaging and chemical preservatives pose risks to both the environment and human health. Consequently, increasing research efforts are directed toward the development of environmentally friendly, non-toxic, and safe packaging materials. In this study, a polyvinyl alcohol (PVA) / pullulan (PUL) / soy hull nanocellulose (SHNC) composite film incorporating 2 % ( w /w) tea polyphenols (TP) was prepared. The composite film exhibited a smooth and dense structure, with hydrogen bonding as the primary intermolecular force. It demonstrated a tensile strength of 52.62 MPa and an elongation at break of 321.45 %. Additionally, the composite film showed enhanced ultraviolet-blocking properties and thermal stability. When immersed in water at 100 °C for 10 min, the film exhibited a dissolution rate of up to 90 %. Notably, it extended the shelf life of dried tofu to 16 days and effectively reduced total volatile basic nitrogen and total viable count levels. The antimicrobial mechanism of the TP-loaded film involved inhibiting Escherichia coli growth by disrupting the bacterial cell membrane, impairing cytoplasmic metabolism, and suppressing protein synthesis. These findings suggest that the PVA/PUL/SHNC/TP composite film holds promise as a sustainable food packaging material.
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DOI
PubMed
高影响因子
Foods
·
2025
·Vol. 14
·期 15
·2750
·
IF 5.1
Xueqing Gao,Xuming Zhuang
Quercetin, a natural polyphenolic flavonoid with antioxidant and anti-allergic properties, is extensively found in foods and holds significant importance for human health. In this study, a simple switch-off fluorescent sensor based on copper nanoclusters (Cu NCs) was proposed for the sensitive determination of quercetin. Glutathione acted as the reducing and protective agent in the synthesized process of Cu NCs via a facile, green one-pot method. As anticipated, the glutathione-capped Cu NCs (GSH-Cu NCs) exhibited favorable water solubility and ultrasmall size. The fluorescence property of GSH-Cu NCs was further enhanced with Al3+ion through the aggregation-induced emission effect. When quercetin was present in the sample solution, the system exhibited effective fluorescence quenching, which was attributed to the internal filter effect. The GSH-Cu NCs/Al3+-based fluorescent sensor showed a good linear relationship to quercetin in the concentration range from 0.1 to 60 μM. A detection limit of 24 nM was obtained. Moreover, the constructed sensor was employed for the successful determination of quercetin in tea samples.
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PubMed
高影响因子
Food Bioengineering
·
2025
·Vol. 4
·期 2
·166-181
·
IF 2.3
Baoming Tian,Xue Zhou,Shuo Pan,Wei Liu
Tea polysaccharides have attracted widespread attention in regulating gut microbiota. A highly efficient enzymolysis and ultrasonic-assisted extraction method was developed and optimized for the extraction of polysaccharides from Zhonghuang No. 1 etiolated-green tea (GTP) with a-single factor and response surface methodology, i.e, with a pH of 5.5, an enzyme concentration of 7%, an ultrasonic time of 2.5 h, a solid-liquid ratio of 1:20 g/mL, and an ultrasonic temperature of 80°C. GTP exhibited a triple helix structure and demonstrated the ability to bind with Congo red. Its surface was observed to be spherical with slender chain fragments. GTP remained intact throughout the simulated in vitro digestive process, which encompassed the oral, gastric, and small intestinal phases. The relative abundance of Bacteroidota was significantly increased, while the Firmicutes/Bacteroidota ratio was reduced following the administration of GTP. The contents of Faecalibacterium and Bacteroides were increased, while the contents of Escherichia_Shigella and Dorea were reduced after GTP treatment. Furthermore, GTP were degraded and utilized by gut microbiota to produce SCFAs and reducing pH value. GTP regulated the composition of the gut microbiota and has the potential to act as a prebiotic, with the possibility of its use as a functional food.
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高影响因子
Foods
·
2025
·Vol. 14
·期 21
·3787
·
IF 5.1
Jinlong Luo,Siyu Liao,Fengjiao Ding,Yuqiao Dai,Zhongying Liu,Ting Yang,Tuo Zhang,Shimao Fang,Yan Li,Lulu Pu,Ke Pan,Wanping Fang,Qiang Shen
‘Qiancha 1’ is an excellent raw material for manufacturing white tea. The effects of different drying parameters on the quality performance of ‘Qiancha 1’ white tea remain poorly understood, which restricts the precise regulation of the quality of ‘Qiancha 1’ white tea. In this research, we systematically investigated the influence of drying temperature (65 °C, 75 °C, and 90 °C) and drying duration (1 h, 2 h, and 3 h) on its non-volatile and volatile compositions, using sensory evaluation, E-tongue, and non-volatilomic and volatilomic analyses. The results showed that the tea sample dried at 65 °C for 3 h had a sweet, mellow, and fresh flavor and scored 95 points, but high-temperature drying (90 °C) could promote increased bitterness and decreased sweetness. High-temperature drying was closely related to a caramel-like and milk-like flavor, which promoted an increase in the content of terpenoids, heterocycle compounds, and esters. During drying, the flavonoid and phenolic acid content increased markedly, contributing to bitterness and astringency, while nucleotides, amino acids, and their derivatives decreased, leading to a reduced umami intensity. A total of 37 key taste-active metabolites were identified, including bitter compounds (e.g., alkaloids), sweet compounds (e.g., phenolic acids), and umami compounds (e.g., nucleotides), whose dynamic changes directly influenced the taste profile of white tea. High-temperature drying promoted an increase in the content of volatile metabolites, such as terpenoids, heterocyclics, and esters, while low-temperature and long-duration drying was beneficial for preserving volatile metabolites like heptanal. 2-Methoxy-3-(1-methylethyl)-pyrazine was determined as the volatile compound with the highest rOAV, providing a sweetness and caramel-like flavor. Overall, the metabolomic analysis revealed that the content of flavonoids and phenolic acids increased after the drying process, which was related to the bitter and astringent taste of the tea liquor. The content of nucleotides, amino acids, and their derivatives decreased after drying, which caused the umami of the tea liquor to weaken. This study provides a theoretical basis for the optimization of the ‘Qiancha 1’ white tea drying process.
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PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Mengcong Zhang,Li Lu,Xiaowen Hu,Chengzhe Zhou,Cheng Zhang,Jiaxin Fang,Linyuan Duan,Bo Zhang,Yuqiong Guo
Goji ( Lycium barbarum L.) leaf tea (LBLT) has gained popularity among consumers owing to its pleasant aroma. Selecting optimal Goji cultivars for tea processing is critical for enhancing the quality of LBLT. This study investigated the aroma characteristics of Goji leaf white tea (LBLWT) and green tea (LBLGT) prepared from 25 Goji lines. LBLWT emitted woody, floral and fruity aromas, while LBLGT exhibited a distinct roasted corn aroma. The integrated application of variable importance in projection, odour activity value and partial least-squares regression analyses led to the identification of key aroma compounds, whose metabolic pathways were also illustrated. Using multi-criteria evaluation methods, excellent Goji lines suitable for processing LBLWT (Z20, Z48 and N1) and LBLGT (Z85, Z20, N1 and Z68) were screened. This study took the lead in adopting multi-criteria evaluation methods, screening high-quality Goji lines and providing theoretical basis for high-quality LBLT processing.
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PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2022
·Vol. 102
·期 15
·7195-7208
·
IF 4.125
Guowen Zhang,Miao Zhu,Yijing Liao,Deming Gong,Xing Hu
BACKGROUND Tea polyphenols have been reported to have the effect of lowering uric acid. However, there are few studies on the inhibitory effects and molecular mechanisms of specific catechins on the urate-metabolizing enzyme xanthine oxidase (XO). In this research, multiple spectroscopic methods and computer simulations were used to determine the inhibitory ability and mechanisms of epigallocatechin gallate (EGCG) and gallocatechin gallate (GCG) on XO. RESULTS Herein, EGCG and GCG reversibly inhibited XO activity in a mixed manner, with IC 50 values of 40.50 ± 0.32 and 33.60 ± 0.53 μmol L −1 , and also decreased the superoxide anion radical (O 2 − ) of the catalytic system by reducing the XO molecule and inhibiting the formation of uric acid. The combination of EGCG or GCG with allopurinol showed synergistic inhibition on XO. The binding of EGCG or GCG to XO with moderate affinity formed a stable complex by hydrogen bonds and van der Waals forces. The presence of EGCG and GCG made the structure of XO more stable and compact. The two inhibitors bound to the vicinity of flavin adenine dinucleotide (FAD) in XO, hindering the entry of substrate; thus the activity of XO was suppressed. CONCLUSION Both EGCG and GCG are excellent natural XO inhibitors, and inhibited the activity of XO by occupying the channel of the substrate to enter the active center and interfering with the dual substrate reaction catalyzed by XO. These findings provide a scientific basis for the application of catechins in dietary supplements and medicines with lowering uric acid effects. © 2022 Society of Chemical Industry.
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PubMed
高影响因子
PLANT JOURNAL
·
2021
·Vol. 109
·期 6
·1489-1506
·
IF 6.486
Mingyue Zhao,Jieyang Jin,Jingming Wang,Ting Gao,Yu Luo,Tingting Jing,Yutong Hu,Yuting Pan,Mengqian Lu,Wilfried Schwab,Chuankui Song
SUMMARY Cold and drought stress are the most critical stresses encountered by crops and occur simultaneously under field conditions. However, it is unclear whether volatiles contribute to both cold and drought tolerance, and if so, by what mechanisms they act. Here, we show that airborne eugenol can be taken up by the tea ( Camellia sinensis ) plant and metabolized into glycosides, thus enhancing cold and drought tolerance of tea plants. A uridine diphosphate (UDP)-glucosyltransferase, UGT71A59, was discovered, whose expression is strongly induced by multiple abiotic stresses. UGT71A59 specifically catalyzes glucosylation of eugenol glucoside in vitro and in vivo . Suppression of UGT71A59 expression in tea reduced the accumulation of eugenol glucoside, lowered reactive oxygen species (ROS) scavenging capacity, and ultimately impaired cold and drought stress tolerance. Exposure to airborne eugenol triggered a marked increase in UGT71A59 expression, eugenol glucoside accumulation, and cold tolerance by modulating ROS accumulation and CBF1 expression. It also promoted drought tolerance by altering abscisic acid homeostasis and stomatal closure. CBF1 and CBF3 play positive roles in eugenol-induced cold tolerance and CBF2 may be a negative regulator of eugenol-induced cold tolerance in tea plants. These results provide evidence that eugenol functions as a signal in cold and drought tolerance regulation and shed new light on the biological functions of volatiles in the response to multiple abiotic stresses in plants.
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高影响因子
Heliyon
·
2023
·
IF 4
Ying Li,Ming-Wang Kong,Nan Jiang,Chen Ye,Xiao-Wei Yao,Xiao-Juan Zou,Hai-Ming Hu,Hong-Tao Liu
Gut microbiota disturbance, autophagy dysregulation, and accumulation of hepatic bile acids (BAs) are essential features of liver injury. Therefore, regulating autophagy and BA metabolism are potential strategies for treating liver diseases. Vine tea has been seen beyond a pleasant tea in food science. Our previous study found that vine tea extract (VTE) intervention alleviated acute liver injury (ALI) by restoring gut microbiota dysbiosis. In this study, we aim to investigate the effect of VTE on carbon tetrachloride (CCl 4 )-induced hepatic autophagy and BA metabolism disorder in mice. The results showed that VTE effectively suppressed CCl 4 -induced liver fibrosis and hepatic autophagy. LC-MS/MS assay suggested that VTE affected fecal BA production by reducing the fecal BA levels and improving cholestasis in ALI mice. Besides, VTE inhibited BA synthesis, promoted BA transport in the liver, and enhanced BA reabsorption in the ileum through the farnesoid X receptor (FXR)-related signaling pathway. The hepatic expressions of Fxr and Abca1 were elevated by VTE. Finally, the depletion of gut microbiota in ALI mice had a negative impact on abnormal autophagy and BA metabolism. It was also noted that the administration of VTE did not provide any additional improvement in this regard. Overall, VTE ameliorated ALI by reversing hepatic autophagy and abnormal BA metabolism, and the beneficial effects of VTE on liver injury depended on the existence of gut microbiota.
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高影响因子
Materials
·
2018
·Vol. 11
·期 5
·710
·
IF 2.467
Guanquan Lin,Huayao Chen,Hongjun Zhou,Xinhua Zhou,Hua Xu
Using butyl methacrylate (BMA) and styrene (St) as monomers and divinylbenzene (DVB) as a crosslinking agent, P(St-BMA) microspheres were prepared by suspension polymerization. Tea tree oil (TTO) microspheres were prepared by adsorbing TTO on P(St-BMA) microspheres. The structure and surface morphology of P(St-BMA) microspheres and TTO microspheres were characterized by Fourier transformed infrared spectroscopy (FTIR), optical microscopy, and Thermogravimetric analysis (TGA). In doing so, the structural effect of P(St-BMA) microspheres on oil absorption and sustained release properties could be investigated. The results show that the surface of the P(St-BMA) microspheres in the process of TTO microsphere formation changed from initially concave to convex. The TTO microspheres significantly improved the stability of TTO, which was found to completely decompose as the temperature of the TTO increased from about 110 °C to 150 °C. The oil absorption behavior, which was up to 3.85 g/g, could be controlled by adjusting the monomer ratio and the amount of crosslinking agent. Based on Fickian diffusion, the sustained release behavior of TTO microspheres was consistent with the Korsmeyer-Pappas kinetic model. After 13 h of natural release, the anti-bacterial effect of the TTO microspheres was found to be significantly improved compared to TTO.
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高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Mingyan Jia,Yixuan Pan,Jianbo Zhou,Mei Zhang
In this work, we developed an optical colorimetric sensor array for the discrimination of Chinese teas. The sensor array was carefully designed based on tea polyphenol induced indicators displacement assay (IDA), using phenylboronic acids with different substituents as the receptors to polyphenols. The accurate identification for polyphenols with different species or concentrations proved the potential of the sensor array. The sensor array successfully distinguished tea samples within different categories, grades and origins, coupling with PLS-DA. This work offered an efficient and rapid method to distinguish teas and tea-related products. Besides, the assay is supposed to be suitable for the identification of other polyphenol-related natural products.
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高影响因子
Food Chemistry-X
·
2022
·
IF 6.443
Ding-Tao Wu,Wen Liu,Qin Yuan,Ren-You Gan,Yi-Chen Hu,Sheng-Peng Wang,Liang Zou
In this study, dynamic variations in physicochemical characteristics of polysaccharides from ‘ Wuyi rock’ tea (WYP) at different simulated digestion and fecal fermentation stages in vitro were studied. Results revealed that physicochemical characteristics of WYP were slightly altered after the simulated digestion in vitro , and its digestibility was about 8.38%. Conversely, physicochemical characteristics of the indigestible WYP, including reducing sugar, chemical composition, constituent monosaccharide, molecular weight, and FT-IR spectrum, were obviously altered after the fecal fermentation in vitro , and its fermentability was about 42.18%. Notably, the indigestible WYP could remarkably modulate the microbial composition via promoting the proliferation of profitable intestinal microbes, such as Bacteroides , Lactococcus , and Bifidobacterium . Moreover, it could also enhance the generation of short-chain fatty acids. The results showed that WYP was slightly digested in the gastrointestinal tract in vitro , but could be obviously utilized by intestinal microbiota, and might possess the potential to improve intestinal health.
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高影响因子
TALANTA
·
2013
·
IF 3.498
Yuanpeng Wang,Ying Sun,Yan Gao,Bo Xu,Qiong Wu,Hanqi Zhang,Daqian Song
The polyaniline-coated magnetic particles with bowl-shaped morphology (Fe 3 O 4 /C/PANI microbowls) were successfully prepared and characterized by scanning electron microscopy, transmission electron microscopy and vibrating sample magnetometry . The prepared microbowls were used as the magnetic adsorbent in dispersive solid phase extraction of five pyrethroids , including cyhalothrin , beta-cypermethrin, esfenvalerate , permethrin and bifenthrin in plain tea drinks. The effects of experiment factors, including amount of Fe 3 O 4 /C/PANI microbowls, pH value, ultrasound extraction time and desorption conditions, were investigated. The extraction recoveries obtained with 8 mg of magnetic microbowls were satisfactory, and the microbowls can be reused after easy washing. Thus, a simple, selective and effective method for the determination of the pyrethroids was established successfully. The results showed that the method had good linearity ( r =0.9992–0.9998), and the limits of detections (LODs) were from 0.025 to 0.032 ng mL −1 . The intra-day and inter-day relative standard deviations (RSDs) were in the range of 2.4–6.1% and 3.5–8.8%, respectively. Recoveries obtained by analyzing the real tea drinks were in the range of 72.1–118.4%.
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高影响因子
危险品化学品经营许可证（带存储）