INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Haiyan Li,Zekun Jin,Nengguo Tao
This study aimed to develop food-grade ethyl cellulose/agarose aerogels for the controlled release of plant essential oils to extend the shelf life of fresh rice noodles. The antibacterial efficacy of ten unsaturated aldehyde essential oils was evaluated against primary spoilage bacteria, including Leuconostoc lactis , Lactococcus lactis , Acinetobacter johnsonii , Exiguobacterium indicum , and Enterobacter sichuanensis . Based on its superior antimicrobial performance, (E)-2-hexenal was selected for encapsulation within ethyl cellulose/agarose aerogels to achieve controlled release and extend the shelf life of fresh rice noodles. Increasing the ethyl cellulose concentration resulted in a more uniform pore size distribution, enhanced mechanical properties and hydrophobicity, and improved controlled release of (E)-2-hexenal in the aerogels. The optimized aerogel, loaded with 0.20 μL/mL of (E)-2-hexenal, exhibited significant antimicrobial activity, effectively inhibiting bacterial growth for 72 h. Moreover, the aerogel formulation effectively preserved the quality of fresh rice noodles by mitigating changes in total plate count, pH, water content, water activity, cooking properties, color, and texture, ultimately extending the product's shelf life by 4 days at 25 °C. These findings highlight the potential of ethyl cellulose/agarose aerogels loaded with (E)-2-hexenal as an innovative preservation strategy for fresh rice noodles, providing a promising method for enhancing food quality and longevity.
研究领域:
食品科学
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微生物学
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DOI
PubMed
高影响因子
PLoS One
·
2025
·Vol. 20
·期 5
·e0324164
·
IF 2.6
Bingbing Cao,Jiali Zhou,Bo Xia,Xiaoqing Li,Rui Wang,Yiqiao Xu,Chunqi Li
Choline has been proven to be effective in maintaining liver function. However, the effect of choline, in combination with other nutrients, on the improvement of non-alcoholic fatty liver disease (NAFLD) remains unclear. This study aimed to investigate the potential effect of the nutraceutical complex containing choline bitartrate, zinc citrate, and dl-α-Tocopheryl acetate on NAFLD in the zebrafish model. The NAFLD model was induced in zebrafish by administering thioacetamide. Experimental groups were established, including a normal control group, the model control group, the positive control group, the nutraceutical complex intervention group, and the choline bitartrate alone intervention group. The intervention was administered to the zebrafish in a water-soluble form, while the positive control group received polyene phosphatidylcholine at a concentration of 50.0 μg/mL. Notably, the protective effect of the nutraceutical complex against NAFLD is more pronounced than that observed with choline bitartrate supplementation alone. The results of transcriptomics and quantitative real-time PCR showed that the potential mechanisms underlying the effects of the nutraceutical complex might involve the upregulation of acacia, acsl1a , fbp2 gene expression, and the downregulation of tbc1d1 gene expression. These results were further validated by western blotting and overexpression experiments. Our findings indicated that choline bitartrate, zinc citrate, and dl-α-Tocopheryl acetate can help improve NAFLD. The results of this study provide evidence for the application of the nutraceutical complex in the improvement of NAFLD.
研究领域:
营养学
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肝脏病理学
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分子生物学
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DOI
PubMed
高影响因子
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
·
2017
·Vol. 106
·期 5
·1827-1841
·
IF 3.189
Yikun Yang,Chunyi Tong,Juchang Zhong,Ruiying Huang,Weihong Tan,Zhikai Tan
Magnetic hyperthermia has been rapidly developed as a potential cancer treatment in recent years. Artificially induced hyperthermia close to a tumor can raise the temperature to 45°C causing tumor cell death. Herein, we introduce a novel method for rapid preparation of anti-cancer magnetocaloric PCL/Fe 3 O 4 mats capable of high-performance hyperthermia using E-jet 3D printing technology. Our 3D printed mats not only maintained the heating efficiency of traditional techniques for magnetic hyperthermia but also prolonged the effective therapy in vivo . When Fe 3 O 4 nanoparticles (NPs) were used in mats at a concentration of 6 mmol/L, 0.07 g PCL/Fe 3 O 4 mats were able to increase the temperature peripherally to 45°C under an alternating magnetic field (AMF) within 45 min. Moreover, the reproducibility experiment indicated that the maximum temperature was achieved following repeated heating and cooling cycles. Cell toxicity tests showed a high cell death rate during one treatment cycle. In vivo experiments indicated clear signs of tumor growth inhibitory and prolonged survival time of tumor-bearing mice after 4 weeks of treatment. The present magnetic mats may be a potential candidate for a novel heat-generating substrate for localized hyperthermia cancer therapy. Furthermore, the main advantage of such implantable magnetic mats is the local and precise delivery of Fe 3 O 4 NPs, ideal for the hyperthermia treatment of easily accessible tumors. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1827–1841, 2018.
研究领域:
生物医学工程
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材料科学
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肿瘤学
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产品关联
DOI
PubMed
高影响因子
Journal of Applied Spectroscopy
·
2022
·Vol. 89
·期 3
·559-566
·
IF 0.816
Gao Ch.,Du H.
Forsythoside E is one of the major secondary metabolites in Forsythia suspensa . The interactions between forsythoside E and two types of cholinesterases, acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) were investigated in PBS buffer (pH 7.40) by using multispectroscopic techniques. Forsythoside E increased the fluorescence intensity of AChE but quenched the fluorescence of BChE. Synchronous fluorescence studies showed that forsythoside E mainly acts on tyrosine residues of AChE and tryptophan residues of BChE. It was also proved that the complex between the compound and cholinesterases formed spontaneously at a stoichiometric ratio of 1:1 via multispectral technology. Finally, forsythoside E inhibited the activities of cholinesterases with similar IC 50 values of 1.08 mM for AChE and 0.92 mM for BChE. The results illuminate the details of the interaction between forsythoside E and cholinesterases.
研究领域:
药理学
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生物化学
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分子生物学
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DOI
高影响因子
WATER SCIENCE AND TECHNOLOGY
·
2022
·Vol. 85
·期 12
·3357-3369
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IF 2.43
Xu Anlin,Liu Wanqun,Chu Leping,Zhang Yunhai,He Yide,Zhang Yongjun
The cathode is the key component in the electro-peroxone process (E-Peroxone), which is popularly constructed with carbon materials. This study developed an innovative method to fabricate a cathode with waste-tire carbon (WTC) whose performance was evaluated for the degradation of tinidazole (TNZ), an antibiotic frequently detected in water. It was found that the addition of WTC in the cathode can significantly promote the yield of H 2 O 2 and the current efficiency: around 2.7 times that of commercial carbon black at the same loading. The critical influencing factors were studied, including the current density, ozone concentration, initial pH value, chlorine ions and initial TNZ concentration. The scavenger tests demonstrated the possible involvement of •OH and . Some transformation products of TNZ were identified with UPLC-MS and the degradation pathway was proposed accordingly. These results demonstrated the potential of WTC for developing E-Peroxone cathodes. The cathode with WTC generated more H 2 O 2 than a commercial carbon black cathode. Reaction conditions including current density, O 3 dose, pH values, initial TNZ concentration and electrolyte were studied. The main reactive oxygen species were probably •OH and . Transformation products were identified and a degradation pathway was proposed.
研究领域:
环境工程
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电化学
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水处理
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材料科学
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药物污染
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DOI
高影响因子
DRUG DELIVERY
·
2020
·
IF 4.902
Pengcheng Zhang,He Tao,Liyang Yu,Lixiao Zhou,Chenggang Zhu
Injectable implants with the ability to form in situ are one of the most promising carriers for the delivery of chemotherapeutic drugs to tumor sites. We have reported a novel injectable in situ-forming implant system composed of n-butyl-2-cyanoacrylate (NBCA), ethyl oleate, along with the sol-gel phase transition. The chemotherapeutic drug-loaded injectable NBCA ethyl oleate implant (INEI) exhibited excellent therapeutic efficacy for local chemotherapy. Herein, we utilize this INEI to carry N, N′-(Sulfonyldi-4,1-phenylene)bis(2-chloroacetamide) (TE-C-5003), which is a selective protein arginine methyltransferase 1 (PRMT1) inhibitor, to treat the lung cancer mice model. The in vitro experiment shows that TE-C-5003 has a good anti-tumor effect on lung cancer (IC50 = 0.7022 µM for A549; IC50 = 0.6844 µM for NCL-H1299) and breast cancer (IC50 = 0.4128 µM for MCF-7; IC50 = 0.5965 µM for MDA-MB-231). Anti-tumor experiments in animal models showed that the average growth inhibition rate of xenografted human lung cancer cells by the TE-C-5003-loaded INEI (40% NBCA) was 68.23%, which is far more than TC-E-5003 alone (31.76%). Our study further confirms that INEI is an effective technique to improve the anti-tumor effect. The druggability of small molecule compounds can be improved with the help of the mentioned technology. Also, TC-E-5003 may be developed as a broad spectrum anti-tumor drug.
研究领域:
肿瘤学
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药学
·
药物递送系统
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DOI
PubMed
高影响因子
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
·
2024
·
IF 3
Chen Ziqiang,Ma Mingtao,Chen Xingguang,Peng Zhengcong,Liu Hua,Lu Jian,Wu Dianhui
The process of aging was crucial in enhancing the aroma quality of Huangjiu. Gas chromatography electronic nose (GC-E-Nose), gas chromatography-mass spectrometry (GC–MS), and gas chromatography-ion mobility spectrometry (GC-IMS) were employed to detect the variations in aroma among five samples aged for varying years (5–11). A comprehensive analysis revealed the presence of 85 compounds in the Shaoxing Huangjiu, twenty of which were identified as crucial contributors to its flavor profile through odor activity values analysis. The volatile aroma components of Huangjiu exhibited notable variations among different aging years. Additionally, the concentration and diversity of key aroma compounds increased with prolonged aging. At the same time, quantitative descriptive sensory analysis showed that aging could enhance the ester and acid aroma of Huangjiu. Moreover, based on variable importance for the projection, seven potential aroma markers such as diethyl succinate, ethyl butyrate, and furfural were screened out, which were the major compounds that caused the flavor differences of aged Huangjiu. The results offer valuable theoretical support for illustrating and regulating the quality of Huangjiu during its aging process.
研究领域:
食品科学
·
分析化学
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产品关联
DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2016
·
IF 4.917
Haiying Huang,Kejia Wu,Aman Khan,Yiming Jiang,Zhenmin Ling,Pu Liu,Yong Chen,Xuanyu Tao,Xiangkai Li
Combined pollutants with polycyclic aromatic hydrocarbons (PAHs) and heavy metals have been identified as toxic and unmanageable contaminates. In this work, Pseudomonas gessardii strain LZ-E isolated from wastewater discharge site of a petrochemical company degrades naphthalene and reduces Cr(VI) simultaneously. 95% of 10 mg L −1 Cr(VI) was reduced to Cr(III) while 77% of 800 mg L −1 naphthalene was degraded when strain LZ-E was incubated in BH medium for 48 h. Furthermore, naphthalene promotes Cr(VI) reduction in strain LZ-E as catechol and phthalic acid produced in naphthalene degradation are able to reduce Cr(VI) abiotically. An aerated bioreactor system was setup to test strain LZ-E’s remediation ability. Strain LZ-E continuously remediated naphthalene and Cr(VI) at rates of 15 mg L −1 h −1 and 0.20 mg L −1 h −1 of 800 mg L −1 naphthalene and 10 mg L −1 Cr(VI) addition with eight batches in 16 days. In summary, strain LZ-E is a potential applicant for combined pollution remediation.
研究领域:
环境微生物学
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生物修复
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环境工程
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DOI
PubMed
高影响因子
ENVIRONMENTAL TOXICOLOGY
·
2024
·
IF 4.4
Ling-Zi Xia,Li-Lan Liu,Jun-Zhe Yue,Zhen-Yu Lu,Ru-Ya Deng,Xi He,Can-Can Li,Burong Hu,Hai-Tao Gao
Objective Phthalates (PEs) could cause reproductive harm to males. A mixture of three widely used PEs (MPEs) was used to investigate the ameliorative effects of zinc (Zn) and vitamin E (VE) against male reproductive toxicity. Methods Fifty male SD rats were randomly divided into five groups ( n = 10). Rats in MPEs group were orally treated with 160 mg/kg/d MPEs, while rats in MPEs combined Zn and/or VE groups were treated with 160 mg/kg/d MPEs plus 25 mg/kg/d Zn and/or 25 mg/kg/d VE. After intervention for 70 days, it's was measured of male reproductive organs' weight, histopathological observation of sperms and testes, serum hormones, PIWI proteins and steroidogenic proteins. Results Compared with control, anogenital distance, testes weight, epididymides weight, and sex hormones were significantly decreased, while the sperm malformation rate was markedly increased in MPEs group ( p < .05); the testicular tissues were injured in MPEs group with disordered and decreased spermatids, and arrested spermatogenesis. PIWIL1, PIWIL2, StAR, CYP11A1 and CYP19A1 were down-regulated in MPEs group ( p < .05). However, the alterations of these parameters were restored in MPEs combined Zn and/or VE groups ( p < .05). Conclusion Zn and/or VE improved steroid hormone metabolism, and inhibited MPEs' male reproductive toxicity.
研究领域:
毒理学
·
生殖生物学
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营养科学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Jiayi Zhang,Zichun Song,Jiuying Cui,Wenbo Li,Song Huang,Bo Ling,Kai Zhang,Xianjiu Liao,Jihua Wei
The human gastrointestinal system contains the conditional pathogen Escherichia coli ( E. coli ), which is a significant cause of vomiting and diarrhea—both of which can be fatal in extreme cases. Given the surge in hospital infections and water pollution, the development of early detection and diagnosis procedures is imperative. This study proposes an electrochemical biosensor for E. coli detection, utilizing nucleic acid aptamers as recognition elements and using E. coli-2571 as an example. It is based on the CRISPR/Cas gene-editing technology. Specifically, the collateral cleavage activity of Cas12a shifts the electrochemical signal after loading the DNA sequence tagged with an electrochemical signal onto the gold electrode, which ultimately enables the identification of E. coli . The biosensor has a detection limit of 13.1 CFU/mL and exhibits a good linear relationship in the concentration range of 10 2 ––10 7 CFU/mL. It was effectively used for the quantitative detection of E. coli in milk and pure water, with a recovery rate ranging from 93.12 % to 98.93 %. The biosensor offers a rapid, precise, affordable, and sensitive tool for E. coli detection. It is anticipated to become a powerful instrument for identifying microorganisms that pose a risk to public health and human health.
研究领域:
分子生物学
·
微生物学
·
生物传感技术
·
公共健康
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产品关联
DOI
高影响因子
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
·
2018
·Vol. 23
·期 2
·1224-1234
·
IF 4.302
Min Jiang,Yufei Yan,Kai Yang,Zhuochao Liu,Jin Qi,Hanbing Zhou,Niandong Qian,Qi Zhou,Tianqi Wang,Xing Xu,Xiangshu Xiao,Lianfu Deng
Bone is the most common metastatic site for breast cancer. The excessive osteoclast activity in the metastatic bone lesions often produces osteolysis. The cyclic-AMP (cAMP)-response element binding protein (CREB) serves a variety of biological functions including the transformation and immortalization of breast cancer cells. In addition, evidence has shown that CREB plays a key role in osteoclastgenesis and bone resorption. Small organic molecules with good pharmacokinetic properties and specificity, targeting CREB-CBP (CREB-binding protein) interaction to inhibit CREB-mediated gene transcription have attracted more considerations as cancer therapeutics. We recently identified naphthol AS-E (nAS-E) as a cell-permeable inhibitor of CREB-mediated gene transcription through inhibiting CREB-CBP interaction. In this study, we tested the effect of nAS-E on breast cancer cell proliferation, survival, migration as well as osteoclast formation and bone resorption in vitro for the first time. Our results demonstrated that nAS-E inhibited breast cancer cell proliferation, migration, survival and suppressed osteoclast differentiation as well as bone resorption through inhibiting CREB-CBP interaction. In addition, the in vivo effect of nAS-E in protecting against breast cancer-induced osteolysis was evaluated. Our results indicated that nAS-E could reverse bone loss induced by MDA-MB-231 tumour. These results suggest that small molecules targeting CREB-CBP interaction to inhibit CREB-mediated gene transcription might be a potential approach for the treatment of breast cancer bone metastasis.
研究领域:
肿瘤学
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分子生物学
·
药理学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Lei Hao,Junjiao Li,Jin Mao,Qi Zhou,Qianchun Deng,Zhaofei Chai,Lei Zheng,Jie Shi
In this work, soybean lecithin (LC) was used to modify β-cyclodextrin (β-CD) with hydrophobic fat chains to become amphiphilic (LC-CD), and vitamin E (VE) was encapsulated in former modified β-CD complexes (LC-CD-VE), the new Pickering emulsions stabilized by LC-CD-VE and LC-CD complexes for the delivery of β-carotene (BC) were created. The surface tension, contact angle, zeta potential, and particle size were used to assess the changes in complexes nanoparticles at various pH values. Furthermore, LC-CD-VE has more promise as Pickering emulsion stabilizer than LC-CD because of the smaller particle size (271.11 nm), proper contact angle (58.02°), and lower surface tension (42.49 mN/m). The interactions between β-cyclodextrin, soybean lecithin, and vitamin E were confirmed using Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), nuclear magnetic resonance (NMR), and thermogravimetric analysis (TGA). The durability of Pickering emulsions was examined at various volume fractions of the oil phase and concentrations of nanoparticles. Compared to the emulsion stabilized by LC-CD, the one stabilized by LC-CD-VE showed superior storage stability. Moreover, for the delivery of BC, Pickering emulsions stabilized by LC-CD and LC-CD-VE can outperform bulk oil and Tween 80 stabilized emulsions in terms of UV light stability, storage stability, and bioaccessibility. This work could offer fresh perspectives on stabilizer alternatives for Pickering emulsion delivery systems.
研究领域:
食品科学
·
胶体化学
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营养学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）