Food Bioscience
·
2026
·
IF 6.2
Xin Zhang,Zhitong Wang,Yijia Hu,Mengjie Yang,Jingyu Hao,Xiuzhen Jia,Wen Zhao,Xuebo Liu,Zhigang Liu,Yanhui Lu
Environmental stressors and probiotics modulate gut microbiota, regulating intestinal barrier function and immune homeostasis. As a potential functional food ingredient, probiotics exhibit various health-promoting properties, including the alleviation of gastrointestinal disorders. This study investigated the exacerbating effects of chronic unpredictable mild stress (CUMS) on dextran sulfate sodium (DSS)-induced colitis and the protective efficacy of Lacticaseibacillus paracasei subsp. paracasei K56 (K56) against this stress-aggravated inflammation. CUMS exposure activated the hypothalamic–pituitary–adrenal (HPA) axis and increased corticosterone levels. Compared with the DSS group, the CUMS + DSS group exhibited higher levels of disease activity index (DAI), histopathological damage, CSF1 expression, and pro-inflammatory cytokines. In mice with CUMS-exacerbated colitis, K56 pretreatment (10 9 CFU/d for 5 weeks) significantly attenuated body weight loss, colon shortening, and DAI. K56 concurrently restored Claudin-1 and Mucin-2 (MUC2) expression, elevated goblet cell counts, and reinforced intestinal barrier integrity. Additionally, K56 reduced pathogenic bacteria and enriched short-chain fatty acid (SCFA)-producing taxa, which increased fecal propionate and butyrate production. K56 also balanced T helper 17 (Th17) and regulatory T (Treg) cells in the colonic lamina propria. Our results indicate that K56 alleviates stress-exacerbated colitis through the microbiota/SCFA/immune axis, supporting its potential as a functional probiotic intervention for managing stress-aggravated intestinal inflammation.
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ACS Sensors
·
2026
·
IF 10.9
Peng Xu,Yanlei Guo,Xin Lv,Shuting Wei,Dan Yang,Renquan Guan,Yuxin Wang,Yingmin Teng,Aiceng Li,Huaqiao Tan,Zhongling Lang,Zheng Lou,Lili Wang,Zhao Zhao
The necessity for high-efficiency, low-power room-temperature (RT) ammonia (NH3) sensors is particularly acute in environmental monitoring, industrial safety, and medical diagnostics. However, conventional chemiresistive sensors frequently require elevated temperatures to initiate reactions, leading to high energy consumption and potential safety risks. Inspired by plant photosynthesis, this paper successfully designs a ternary S-scheme heterojunction for a photo-activated RT NH3 sensor. The design effectively mimics the efficient charge transfer process in photosynthesis, thereby significantly promoting the separation and migration of photogenerated carriers and enhancing the sensing performance. Experimental results demonstrate exceptional performance under illumination: a high response value of 1679% (representing a 20-fold improvement over the dark state), rapid response and recovery times of 2.1 s and 1.9 s, respectively, along with outstanding selectivity, excellent stability, and a detection limit as low as 17 ppb. This work demonstrates a novel, efficient RT photo-activated NH3 sensor and provides innovative insights into designing biomimetic, smart gas-sensing materials and devices.
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ACS Nano
·
2026
·Vol. 20
·期 34
·23716-23728
·
IF 17.3
Zeng Qing,Tamtaji Mohsen,Zhou Rui,Zhou Tao,Guo Zhengxiao,Chen GuanHua,Tse Edmund C. M.
Electrocatalytic oxidation of glycerol toward high-value
C3 products
on Pd-based catalysts presents a promising strategy for upgrading
and converting glycerol. However, C3 intermediates often adsorb too
tightly on Pd-based catalyst surfaces during glycerol oxidation, leading
to C–C bond cleavage. Herein, cubic-shaped PdBi nanoparticles
with tunable Ni doping levels are successfully synthesized through
a one-step solvothermal method. Owing to the downshift of d-band center
originating from the introduction of Bi and Ni as well as the cubic
shape, the PdBiNi ternary nanocubes (NCs) exhibit excellent electrocatalytic
performance for the glycerol oxidation reaction (GOR). Specifically,
Pd 83 Bi 12 Ni 5 NCs with the lowest d-band
center exhibit a specific activity of 61.12 mA cm geo –2 and a mass activity of 5.34 A mg Pd –1 . Notably, the Faradaic efficiency (FE) toward C3
products exceeds 97%. In situ Fourier transform infrared spectroscopy
and density functional theory calculations show that key C3 intermediates
are easily desorbed from the catalyst surface, thereby suppressing
C–C bond cleavage and thus preventing overoxidation into low-value
products. In addition, Pd 83 Bi 12 Ni 5 NCs possess long-term durability over the course of 16 cycles (1
h/cycle) with an FE for C3 products between 92.5% and 97.6% upon scale-up
deployment in a flow electrolyzer, validating its feasibility in practical
operations.
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Foods
·
2026
·Vol. 15
·期 17
·3085
·
IF 6
Xiao Yang,Song Xu,Ying Tong,Jichu Luo,Xixing Fang,Jiaxing Du,Changyuan Zhou,Guangnian Hu,Bao Yang,Qisong Zhang
(1) Background: Ulcerative colitis (UC) is a chronic inflammatory bowel disease associated with gut microbiota dysbiosis and metabolic perturbations. Although Liubao tea (LBT) has gastroprotective benefits, the precise mechanisms by which LBT extract (LBTE) alleviates UC by orchestrating microbial and metabolic homeostasis remain poorly understood. (2) Methods: A DSS-induced UC mouse model was used to evaluate LBTE efficacy. Serum pharmacochemistry, untargeted metabolomics, 16S rRNA sequencing, and targeted SCFA metabolomics were integrated to characterize absorbable active constituents, metabolic shifts, and gut microbiota landscapes. SCFA- and arachidonic acid metabolism-related targets were validated by RT-qPCR and Western blotting. PGF models and FMT were used to assess the causal role of gut microbiota in LBTE-mediated efficacy. (3) Results: LBTE preserved colon length and mucosal integrity while reducing IL-6, TNF-α, IL-1β, and oxidative stress. It enriched SCFA-producing genera and increased colonic butyric and valeric acids, activating GPR41/GPR109A signaling, upregulating ZO-1 and occludin, and strengthening the intestinal barrier. LBTE also downregulated PTGS2 and ALOX5, restored PTGS1 and CYP3A11, and inhibited NF-κB signaling. These effects were weakened in PGF mice but reproduced by FMT from LBTE-treated donors, confirming microbiota-dependent protection. (4) Conclusions: LBTE may serve as a complementary strategy for UC prevention and management.
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ELECTROPHORESIS
·
2025
·Vol. 46
·期 21
·1576-1587
·
IF 2.5
Tao Huang,Chunsu Liang,Xiaomei Ling
High-performance capillary electrophoresis (CE) has been widely applied in the analysis of organic acids, especially monocarboxylic acids (MAs), but no studies on the CE analysis of dissociated states of MAs have been reported. Here, 15 MAs were analyzed by the newly developed universal interface-induced current detector (IICRD). Two current signal peaks were observed in current electrophoretograms (CR-EGs), whereas only one peak with low sensitivity can be obtained by diode array detector (DAD). The current signal peaks of MAs were accurately identified by adding a charge-neutral marker and calculating by p K a . The qualitative analysis indicated that the two current signal peaks of MAs were charge-neutral forms [MA ± ] and monovalent anions [MA − ] in order. Quantitative analysis showed that CE-IICRD can enhance the sensitivity of MA analytes to 40 µM in limit of detection (LOD), with a linear range from 10 −6 to 10 −2 M. Furthermore, interactions between different dissociated states of MAs and monocarboxylate transporter 1 (MCT1) were studied by combined application of nonimmobilized cell CE (NICCE) method for the first time. The binding kinetic parameters and mole number of MCT1 on cell membranes can also be obtained. Besides, competitive binding experiments proved that BAY-8002 (MCT1-specific inhibitor) and lactic acid shared the same binding site. CE-IICRD provides a new method to reveal the interaction between different dissociated forms of MAs or other biomolecules and essential receptors.
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PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
·
2025
·Vol. 122
·期 44
·e2513015122
·
IF 9.1
Anqi Li,Dong Tong,Benjamin Ohlstein,Zheng Guo
Recent work suggests that asymmetric segregation of preexisting and newly synthesized canonical histone 3.1 (H3.1), but not variant histone 3.3A (H3.3A), plays an important role in the asymmetric outcome of Drosophila germline stem cell (GSC) and intestinal stem cell (ISC) divisions. However, this finding relied on the Gal4/UAS system and Flp-out technology to swap expression of exogenous fluorescent proteins—an approach that decouples H3.1 expression from the cell cycle and may be prone to artifacts. Here, by photoswitching photoconvertible H3-Dendra2 proteins expressed under their native controls in living flies, we find that preexisting and newly synthesized H3.1 and H3.3A are symmetrically segregated in dividing ISCs and somatic cyst stem cells (CySCs) in male testes. While H3.3A was found to be symmetrically segregated during GSC divisions, analysis of the H3.1 segregation in GSCs was complicated by our unexpected finding that H3.1-Dendra2 expressed under its normal control within the context of a histone transgene is not detectable in GSCs, owing to regulatory elements in the 3’UTR that mediate translational repression by the RNA-binding proteins Nanos and Pumilio. Nevertheless, when this repression is obviated by using a 3’UTR devoid of such elements, H3.1-Dendra2 is expressed in GSCs and symmetrically segregated without affecting lineage outcomes. We obtained similar results when the coding sequence of H3.3A was edited to encode H3.1 in the context of an H3.3A-Dendra2 knock-in allele. Our findings thus challenge the prevailing model of asymmetric histone segregation and reveal an unexpected molecular mechanism of posttranscriptional repression of H3.1 in GSCs.
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Chinese Medicine
·
2025
·Vol. 20
·期 1
·1-23
·
IF 5.7
Xu Jin-Di,Gao Xiao-Qin,Zhong Rong-Ling,Zhou Jing,Wang Ting,Chen Can,Yao Wei-Feng,Geng Ting,Zhang Yi,Li Song-Lin,Zhang Li
Background Shi–Zao–Tang (SZT), a classical formula of Traditional Chinese Medicine orally used for treating malignant ascites effusion (MAE), is made by mixing the powder of Kansui Radix (KR), Euphorbiae Pekinensis Radix (EPR) and fried Genkwa Flos with the decoction of Jujubae Fructus. According to Chinese Pharmacopoeia, vinegar-processed KR and EPR should be used in oral administration. However, toxicity and efficacy of SZT containing vinegar-processed KR and EPR (VSZT) versus SZT in MAE rats, and the potential mechanisms of VSZT against MAE, remain unknown. Here, we comparatively studied the quality, toxicity and efficacy of SZT and VSZT, and explored the potential mechanisms of VSZT against MAE. Methods Main components in SZT and VSZT were quantified by liquid chromatographic coupled with triple quadrupole tandem mass spectrometry. The intestinal toxicity and efficacy of SZT and VSZT were comparatively investigated in MAE rats. Specially, intestinal toxicity was evaluated by intestinal barrier function, histopathology and oxidative damage. The efficacy was investigated by amount of ascites, indices in excretion, intestinal motility and inflammation. The potential mechanisms of VSZT treats MAE were explored through integration of metabolomics, 16S rRNA and Western blotting analysis. Results VSZT contains less 3- O - EZ and more ingenol than SZT. VSZT showed reduced intestinal toxicity than SZT in MAE rats. Both SZT and VSZT indiscriminately decreased the amount of ascites and ascitic inflammatory cytokines, promoted urination and defecation, increased fecal water content and intestinal motility. VSZT reversed endogenous metabolism and gut microbiota disorders, down-regulated colonic cAMP, PKA, p-CREB/CREB and AQP3, as well as mesenteric p-VEGFR2/VEGFR2, p-SRC/SRC, and p-VE-cadherin/ VE-cadherin in MAE rats. Conclusion VSZT preserved the efficacy of SZT on MAE with lower intestinal toxicity. VSZT increased water excretion and decreased MAE formation to alleviate MAE through regulating gut microbiota, restoring tryptophan and tyrosine metabolism disorders, and affecting cAMP-PKA-CREB-AQP3 and VEGFA-VEGFR2-SRC-VE-cadherine pathway.
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JOURNAL OF APPLIED MICROBIOLOGY
·
2025
·
IF 3.2
Chen Yang,Yang Jian-xia,Yv Tian,Zhang Xin-rui,Gong Gui-zhen,Zhu Yuan-ting,Yao Jin,Wu Yi,Zhang Yv-han
AimsThis study aimed to investigate the capacity of lactic acid bacteria (LAB) isolated from yak calves to metabolize xylo-oligosaccharides (XOS) and to identify potential probiotic strains that could enhance the gut health of yak calves.Methods and ResultsLAB isolates were recovered from fifteen fecal samples collected from yak calves. Their capacity to utilize XOS as the sole carbon source was evaluated, and their metabolic characteristics, molecular mechanisms, and functional properties associated with XOS metabolism were examined through integrated phenotypic and genomic analyses. Consequently, forty-one LAB isolates were identified from ten of the fifteen yak calves and tested for XOS utilization. Among these, 51.22% (21/41) exhibited species- and strain-specific XOS utilization, including Limosilactobacillus mucosae (10 isolates), Lactobacillus brevis (10 isolates), and Lactiplantibacillus plantarum (1 isolates). L. mucosae YT200 showed the highest acid production (pH 4.50) and short-chain fatty acid accumulation, particularly acetic acid (745.00 ± 16.24 mM). Genomic analysis revealed three gene clusters encoding GH43 α-L-arabinofuranosidase, GH51 α-L-arabinofuranosidase, and GH43 β-xylosidase, enabling the hydrolysis of arabinosyl-substituted and linear XOS. Strain YT200 also demonstrated antagonism against common gut pathogens including Escherichia coli and Staphylococcus aureus, sensitivity to seven antibiotics, and no transferable resistance genes.ConclusionThis study reveals the mechanistic basis of XOS metabolism in L. mucosae YT200 and identifies L. mucosae YT200 as a promising candidate for synbiotic development.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Zhengqi Zha,Xiaoning Wang,Guoqing Wang,Hongping Yin,Hang Wang
Acetic acid has been shown to be effective in chronic kidney disease (CKD). However, it is a low-molecular-weight compound that allows it to be absorbed in the upper digestive tract so that it cannot function in colon. To overcome these deficiencies, an acetate-releasing xylan derivative, xylan acetate ester (XylA), was synthesized and selected in this study for its potential in the treatment of CKD. IR, NMR and HPGPC were used to characterize the structure of XylA and its antinephritic effects was evaluated in vivo. The results showed that acetate was successfully grafted onto the C-2 and C-3 positions of xylan and with a molecular weight at 69157 Da. XylA treatments could relieve the symptoms of CKD in an adenine-induced chronic renal failure (CRF) model and an adriamycin-induced focal segmental glomerulosclerosis (FSGS) model in SD rats. Further study indicated that XylA could upregulate the short-chain fatty acids (SCFAs) in vitro and vivo . Nevertheless, the relative abundance of Phascolarctobacterium in colon was increased after XylA treatment. XylA could upregulate G-protein-coupled receptor 41 (GPR41) expression, inhibit glomerular cell apoptosis and promoting proliferation. Our study expands the application of xylan and provides a new idea for the treatment of CKD with acetic acid.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Yongchang Tian,Rong Zhang,Binbin Guan,Yaowei Zhu,Li Chen
The compounding of polysaccharide macromolecules and antibacterial agents always has been the preferred strategy to prepare antibacterial products, attracting increasing interest. Herein, a novel acid-responsive oxidized dextran-based nanoplatform ( OTP NP ) has been fabricated for photodynamic antibacterial therapy by combing photosensitizer monoaminoporphyrin ( TPP-NH 2 ) with oxidized dextran ( ODex ) via the Schiff Base reaction. OTP NP of about 100 nm is composed of an inner hydrophobic core of 30 nm and peripheral polysaccharide macromolecules . The OTP NP killed 99.9 % of E. coli and S. aureus within 1.5 light cycles at a concentration of 200 μg/mL. Concurrently, OTP NP exhibited excellent cytocompatibility at a concentration of 1 mg/mL (about 5 folds bactericidal concentration). Particularly, except for the recognized antibacterial mechanism of photodynamic therapy, a novel mechanism of bacterial membrane damage was discovered: the bacterial cell membrane was peeled off and formed spherical particles that aggregated around the bacteria to accelerate bacterial apoptosis under the combined action of ROS and nanomaterials. Moreover, the slightly soluble drug levofloxacin ( Lev ) as a model drug was loaded into OTP NP to test its carrier function, providing a practicable strategy to design multifunctional polysaccharide-based photodynamic antibacterial materials.
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FOOD CHEMISTRY
·
2023
·
IF 8.8
Wenxia Tian,Xiaofang Liu,Jia Zheng,Yi Ma,Huibo Luo,Xiaogang Luo,Changjun Hou,Danqun Huo
Quickly discriminating different segmented Baijiu can directly control its grade and indirectly affect the quality of the finished Baijiu. A fluorescence sensor array was constructed based on PEI-terminated silver nanoparticles and lanthanide metal ions (PEI@Ag NPs@Ln). Ag NPs were stably dispersed in the PEI-woven network, initially accompanied by excellent fluorescence signals. Organic molecules disrupted the PEI structure and dragged the Ag NPs out. The free Ag NPs sintered or aggregated with the diffusion, resulting in fluorescence quenching. The three lanthanide ions speed up the process, allowing different organic molecules to exhibit more distinct signals. Thus, this sensor was used to map 11 organic molecules’ fingerprints and to discriminate segmented Baijiu. The whole process takes only 2 min. With the assistance of pattern recognition, segmented Baijiu from three cellars were successfully discriminated. Fast, effective and simple are highlights, which opens up its practical application potential in the detection field.
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JOURNAL OF ENVIRONMENTAL MANAGEMENT
·
2023
·
IF 8.7
Minghui Liu,Yutong Zhao,Quanzhong Cheng,Bingyang Tian,Ming Tian,Jian Zhang,Hui Zhang,Tianyan Xue,Tao Qi
Residual oil containing platinum group metals (PGMs), which is under-researched, can easily pose resource waste and environmental risks. PGMs feature as scarce strategic metals, and inorganic acids and potassium salts are also considered valuable. An integrated process for the harmless treatment and recovery of useful resources from residual oil is proposed herein. This work developed a zero-waste process based on the study of the main components and characteristics of the PGM-containing residual oil. The process consists of three modules: pre-treatment for phase separation, liquid-phase resource utilisation, and solid-phase resource utilisation. Separating the residual oil into liquid and solid phases allows for the maximum recovery of valuable components. However, concerns about the accurate determination of valued components emerged. Findings revealed that Fe and Ni are highly susceptible to spectral interference in the PGMs test when using the inductively coupled plasma method. After studying 26 PGM emission lines, Ir 212.681 nm, Pd 342.124 nm, Pt 299.797 nm, and Rh 343.489 nm were reliably identified. Finally, formic acid (81.5 g/t), acetic acid (117.2 kg/t), propionic acid (291.9 kg/t), butyric acid (3.6 kg/t), potassium salt (553.3 kg/t), Ir (27.8 g/t), Pd (10960.0 g/t), Pt (193.1 g/t), and Rh (109.8 g/t) were successfully obtained from the PGM-containing residual oil. This study provides a helpful reference for the determination of PGM concentrations and high-value utilisation of PGM-containing residual oil.
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