MOLECULAR PHARMACEUTICS
·
2017
·
IF 4.44
Dandan Liu,Jinyu Li,Bingchao Cheng,Qingyin Wu,Hao Pan
This study is focused on further understanding the characteristics of chitosan-N-acetylcysteine surface-modified nanostructured lipid carriers (CS-NAC-NLCs) in their interaction with ocular mucosa. Coumarin-6 (C6)-labeled NLCs, including uncoated NLCs, chitosan hydrochloride (CH)-, and CS-NAC-coated NLCs, were developed using a melt-emulsification technique and subsequently decorated with different types or portions of chitosan derivatives. Mucoadhesion was evaluated ex vivo using a flow-through process with fluorescence detection. The results demonstrated that the presence of CS-NAC on the C6-NLC surface provided the most obvious enhancement in adhesion due to the formation of both noncovalent (ionic) and covalent (disulfide bridges) interactions with mucus chains. Meanwhile, the concentration of CS-NAC in the formulation positively influenced the viscosity of the nanoparticles and hence prolonged their retention in the ocular tissue. Transcorneal penetration studies revealed that CS-NAC-NLC particles were able to penetrate through the entire corneal epithelium primarily via a transcellular route. The transport depth and velocity strongly relied on the modification material and the particle size. Ex vivo fluorescence imaging and in vivo ocular distribution investigations showed that C6 was broadly distributed in rabbit eye tissues and absorbed by aqueous humor after CS-NAC-NLC instillation. In relation to C6 eye drops, CS-NAC-NLCs achieved considerably higher Cmax (4.01-fold), MRT0-∞ (1.87-fold), and AUC0-∞ (16.29-fold) in the aqueous humor. Moreover, the increase in drug absorption was greater in the cornea than in the conjunctiva. Thereby, it is possible to draw a conclusion that CS-NAC-NLCs presented great potential for drug application to the front portion of the eye.
研究领域:
药剂学
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眼科研究
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
Frontiers in Chemistry
·
2024
·
IF 3.8
Sun Jingxuan,He Lina,An Qi,Ye Xu,Ma Jinjie,Yan Jing,Xie Xiaoqi,Sun Xiangyu,Niu Yumei,Cao Wenxin
Introduction: Facial nerve injury significantly impacts both the physical and psychological] wellbeing of patients. Despite advancements, there are still limitations associated with autografts transplantation. Consequently, there is an urgent need for effective artificial grafts to address these limitations and repair injuries. Recent years have witnessed the recognition of the beneficial effects of chitosan (CS) and graphene in the realm of nerve repair. Dental pulp stem cells (DPSCs) hold great promise due to their high proliferative and multi-directional differentiation capabilities.Methods: In this study, Graphene/CS (G/CST) composite tubes were synthesized and their physical, chemical and biological properties were evaluated, then DPSCs were employed as seed cells and G/CST as a scaffold to investigate their combined effect on promoting facial nerve injury repair.Results and Disscussion: The experimental results indicate that G/CST possesses favorable physical and chemical properties, along with good cyto-compatibility. making it suitable for repairing facial nerve transection injuries. Furthermore, the synergistic application of G/CST and DPSCs significantly enhanced the repair process for a 10 mm facial nerve defect in rabbits, highlighting the efficacy of graphene as a reinforcement material and DPSCs as a functional material in facial nerve injury repair. This approach offers an effective treatment strategy and introduces a novel concept for clinically managing facial nerve injuries.
研究领域:
生物医学工程
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组织工程
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干细胞研究
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神经科学
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DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2025
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IF 3.5
Jian-xia Yang,Hai-ying Guo,Kexin Zhang,Yong Zuo,Yuan-ting Zhu,Yan Zeng,Gui-zhen Gong,Lin-zhi Zeng,Yi-xin Wang,Lan-yue Chen,Yang Chen
BACKGROUND Rabbits often suffer from diarrhea post-weaning, necessitating eco-friendly alternatives to banned antibiotic feed additives. This study aimed to investigate the effect of xylo-oligosaccharide (XOS) on the in vitro proliferation of 94 lactic acid bacteria (LAB) strains derived from the gut of rabbits. RESULTS XOS significantly enhanced the growth of 34 LAB strains (36.2%, 34/94), with Lactobacillus sp. YT155 demonstrating robust XOS metabolism. The genome of strain YT155 harbored xylABCPRT and xylCFGHT gene clusters, encoding intracellular β -xylosidase for XOS degradation. Additionally, strain YT155 exhibited tolerance to varying conditions, including temperature (37–50 °C), pH (5.0–10.0) and salinity (2–6%), and gastric and intestinal fluids. Strain YT155 also showed strong antagonistic activity against Escherichia coli and Staphylococcus aureus , and high antioxidant capacity with DPPH and hydroxyl radical scavenging rates of 100.39 ± 1.82% and 34.09% ± 1.57%, respectively. Furthermore, strain YT155 was sensitive to nine commonly used antibiotics and lacked transferable resistance genes. CONCLUSION XOS is a viable prebiotic for improving rabbit gut health by promoting beneficial LAB. Lactobacillus sp. YT155's ability to metabolize XOS and its excellent probiotic characteristics suggest it could be combined with XOS as a synbiotic to modulate gut microbiota. © 2025 Society of Chemical Industry.
研究领域:
微生物学
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动物营养
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肠道健康
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益生菌与益生元
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食品科学
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生物技术
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产品关联
DOI
PubMed
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
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2021
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IF 7.328
Xiaohong Li,Xiongfa Ji,Kun Chen,Xi Yuan,Zehua Lei,Muhammad Wajid Ullah,Jun Xiao,Guang Yang
The present study aimed to develop a new drug delivery system with efficient drug loading and sustained drug release for potential application in transarterial chemoembolization (TACE). The porous polyvinyl alcohol microspheres (PPVA MS) were prepared by a combination of inverse emulsification and thermal-induced phase separation (TIPS) method, this was followed by the grafting polymerization of sodium 4-styrene sulfonate (SSS) onto the PPVA MS to obtain the grafted PPVA-g-PSSS MS. The prepared PPVA MS showed a well-defined spherical shape with ‘honeycomb-like’ porous structure, which could be readily tailored by adjusting the quenching temperature. In vitro biocompatibility analysis indicated the non-cytotoxic and hemocompatible nature of PPVA MS. The porous structure and presence of ionically charged groups in the PPVA-g-PSSS MS favoured the loading of cationic doxorubicin (DOX) onto the MS through ionic-interactions and demonstrated a sustained drug release pattern. Moreover, the cytotoxicity of DOX-loaded PPVA-g-PSSS ( [email protected] ) MS against HepG2 cells and the intracellular uptake of DOX demonstrated the potent in vitro antitumor activity. Furthermore, the central auricular artery embolization in rabbits showed that both the PPVA-g-PSSS and [email protected] MS could occlude the auricular arteries and induced superior embolization effects, such as progressive ear appearance changes, irreversible parenchymal damage and fibrosis, and ultrastructural alternations in endothelial cells. Besides, the DOX fluorescence was distributed around the embolized arteries, without decreasing its intensity when prolonged embolization up to 15 days. These findings suggest that the newly developed [email protected] MS could be employed as a promising drug-loaded embolic agent for the treatment of hepatocellular carcinoma.
研究领域:
药剂学
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生物医学工程
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肿瘤学
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DOI
PubMed
高影响因子
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
·
2014
·Vol. 103
·期 4
·1509-1519
·
IF 2.841
Fei Sun,Shu Pan,Hong-Can Shi,Fang-Biao Zhang,Wei-Dong Zhang,Gang Ye,Xing-Chen Liu,Si-Quan Zhang,Chong-Hao Zhong,Xiao-Long Yuan
Decellularization techniques have been widely used as an alternative strategy to produce matrices for organ reconstruction. This study investigated the impact of a detergent-enzymatic decellularization protocol on the extracellular matrix integrity, mechanical properties, and biocompatibility of decellularized tracheal matrices from rabbits. The tracheas of New Zealand white rabbits were decellularized using a modified detergent-enzymatic method (DEM). Antigenicity, cellularity, glycosaminoglycan content, DNA content, histoarchitecture, and mechanical properties were monitored during processing. The surface ultrastructure of the matrix was examined by scanning electron microscopy (SEM). Bioengineered and control tracheas were then implanted in major histocompatibility complex-unmatched rats (xenograft) heterotopically for 7, 15, and 30 days. Structural and functional analysis was performed after transplantation. The results showed that seven cycles of decellularization removed most of the cells and eliminated antigenicity. Histological and molecular biology analysis demonstrated that most of the cellular components and nuclear material were removed. SEM analysis revealed that the decellularized matrices retained the hierarchical structure of the native trachea, and biomechanical tests showed that decellularization did not significantly influence the mechanical properties. Seven, 15 and 30 days after implantation, decreased ( p < 0.01) inflammatory reactions were observed in the xenograft models for decellularized matrices compared with control tracheas. No increases in IgM or IgG content were observed in rats that received bioengineered tracheas. In conclusion, this work suggests that seven cycles of the DEM generates a bioengineered rabbit tracheal matrix that is structurally and mechanically similar to native trachea. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 1509–1519, 2015.
研究领域:
组织工程
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生物医学材料
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免疫学
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机械工程
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Jun Wu,Yaguang Han,Qiwei Fu,Yuping Hong,Lexiang Li,Jia Cao,Haobo Li,Yi Liu,Yi Chen,Jun Zhu,Jiahua Shao,Peiliang Fu,Haishan Wu,Daxiang Cui,Bo Wang,Yiqin Zhou,Qirong Qian
Articular cartilage (AC) lesions are common but remain a great challenge for researchers and clinicians mainly due to their poor self-healing capability. Decellularized cartilage extracellular matrix (dCECM) derived from natural cartilage, and the incorporated growth factors have been used for potential chondrocyte induction and cartilage tissue regeneration. Moreover, infrapatellar fat pad adipose-derived stem cells (IPFP-ADSCs) have been proven to be an optimal source of seeded cells for AC lesion restoration. Therefore, the therapeutic role of dCECM-incorporated bioink with IPFP-ADSCs loaded in 3D (three-dimensional) scaffolds remain to be explored using an osteochondral defect model. In this work, we successfully extracted IPFP-ADSCs and isolated dCECMs, which were then mixed in a temperature-responsive hydrogel to form a 3D bioink. The 3D bioprinting scaffolds printed with dCECM successfully facilitated chondrogenic differentiation-related growth factor expression in IPFP-ADSCs. Most importantly, implantation of the 3D dCECMs with an IPFP-ADSC-loaded scaffold was capable of providing a favorable cell matrix for stem cell proliferation and promoted chondrogenic differentiation, thereby greatly enhancing successful cartilage repair in rabbit cartilage defects.
研究领域:
组织工程学
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再生医学
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骨科研究
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DOI
高影响因子
ACS Biomaterials Science & Engineering
·
2019
·
IF 4.511
Kun Lei,Qi Zhu,Xinling Wang,Haijun Xiao,Zhen Zheng
Biocompatible bone adhesive is a kind of promising biomaterial in orthopedic and trauma surgery. However, despite the urgent need, there is no appropriate system that can meet all the requirements for bone adhesive. In this study, a porous polyurethane adhesive (PUA) was fabricated, and the effects of water, polyisocyanate, and β-tricalcium phosphate (β-TCP) on the physicochemical and mechanical properties of PUAs were investigated. By varying the water content, PUAs with different surface morphologies and porosities were prepared. The adhesion strength and mechanical property of the adhesives could be enhanced by the addition of polyisocyanate and β-TCP. The adhesion strength of the PUA to bone was 2 times higher than that of clinical poly(methyl methacrylate) bone cement. The in vitro cell culture and attachment assays indicated good biocompatibility of the PUAs. In vivo analysis in a rabbit model demonstrated that the porous structure of the PUA could facilitate the growth of cells and bone tissues.
研究领域:
生物材料
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骨科手术
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组织工程
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2019
·
IF 8.355
Chunyu Yang,Weicheng Huang,Yan Gao,Zhao Liu,Na An,Wei Mu,Qinmin Pan,Bin Yang,Chongshen Guo,Xiaojun Han
Although near-infrared (NIR)-mediated phototherapy has achieved great progresses, mice with subcutaneous tumors are employed by nearly all previous phototherapy studies, which is largely different from the clinical human tumors. A large animal model with orthotopic carcinoma is highly demanded before clinical practice. This work designs a non-stoichiometric photoactive substance of BiPO 4−x for the phototherapy on both of subcutaneous tumor-bearing mice and orthotopic VX2 tumor-bearing rabbit, aiming for perspective clinic prediction. Oxygen defective BiPO 4−x is a brand-new phototherapeutic agent with desirable NIR-harvesting properties, which facilitate production of hyperthermia and reactive oxygen species (ROS), and thus contribute to a synergistic photothermal/photodynamic therapy (PTT/PDT) outcome. The photoabsorption mechanism of BiPO 4−x predicted by density functional theory reveals the emergence of abundant middle band transition as oxygen defect introduction, accounting for a NIR absorption resultantly. The antitumor evaluation on large animal model of rabbits is realized with ultrasound (US) technique and contrast-enhanced ultrasound (CEUS) for deep orthotopic tumor examination. To be excited, the effective elimination of orthotopic rabbit tumors is successfully achieved. Hence, this work advances tumor phototherapy further to the macroanimal model, which makes it to be more clinically relevant.
研究领域:
肿瘤学
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生物医学工程
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材料科学
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产品关联
DOI
高影响因子
Photoacoustics
·
2021
·
IF 8.484
Lejian Lin,Zhihua Xie,Mengqi Xu,Yabin Wang,Sulei Li,Ning Yang,Xiaojing Gong,Ping Liang,Xu Zhang,Liang Song,Feng Cao
Current intravascular imaging modalities face hurdles in the molecular evaluation of progressed plaques. This study aims to construct a novel hybrid imaging system (intravascular ultrasound/intravascular photoacoustic, IVPA/IVUS) via RGDfk peptide-targeted nanoparticles for monitoring angiogenesis in progressed atherosclerotic plaques in a rabbit model. An atherosclerotic rabbit model was induced by abdominal aorta balloon de-endothelialization followed by a high-fat diet. A human serum albumin (HSA)-based nanoprobe modified with RGDfk peptide was constructed by encapsulating indocyanine green (ICG) via electrostatic force (ICG-HSA-RGDfk NPs, IHR-NPs). A hybrid intravascular imaging system that combined IVUS and IVPA was self-assembled for RGDfk visualization within atherosclerotic plaques in the rabbit abdominal aorta. Through IHR-NPs and the hybrid IVUS/IVPA imaging platform, multiple comprehensive pieces of information on progressed plaques, including anatomical information, composition information and molecular information, can be obtained simultaneously, which may improve the precise diagnosis of plaque characteristics and the evaluation of early interventions for atherosclerosis.
研究领域:
医学影像
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分子生物学
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心脏病学
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纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Sizhu Tian,Xuwen Li,Jia Jiang,Li Tang,Hanqi Zhang,Yong Yu,Ziwei Zhang
Water soluble copper sulfide (CuS) nanoparticles were synthesized using l -cysteine as the ligand. Multiple biotins were conjugated to the antibody of rabbit IgG, and the streptavidin was attached to the CuS nanoparticles. The Cu 2+ ions enclosed in the nanoparticles were used as the electron spin resonance (ESR) probes and detected with ESR spectrometer. The immunoassay reaction was resulted in the formation of the coating antibody attached to the microplate well, the detecting antibody labeled with the biotins, and the streptavidin attached to the CuS nanoparticles. After the immunoassay reaction was performed, large amount of Cu 2+ /Cu + ions inside the nanoparticles were released with the help of diethyldithiocarbamate (DDC) and the Cu 2+ -DDC complex formed. The Cu 2+ -DDC complex was extracted into n -butanol, which was used as the analytical sample. Both ESR and UV–vis signals were collected for the analytical sample. The double logarithm standard curve was well simulated with a linear regression equation. The limits of detection of the rabbit IgG were 1.76 pg/mL and 2.36 pg/mL by ESR and UV–vis method, respectively. The detection range using ESR as the detector was from 8.8 pg/mL to 500 ng/mL, covering almost 5 magnitude orders of the rabbit IgG concentrations. The rabbit serum was analyzed and the rabbit IgG concentration was found to be 7.76 mg/mL. The reproducibility of the present method was good enough with the intra-assay error within 3.4 % and the inter-assay error within 11.2 %. The spiked serum samples were analyzed and the experimental results indicated that the recoveries were from 108.2 to 113.7%.
研究领域:
生物化学
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分析化学
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纳米技术
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产品关联
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2017
·Vol. 410
·期 6
·1817-1824
·
IF 3.431
Jiang Jia,Tian Sizhu,Wang Kun,Wang Yang,Zang Shuang,Yu Aimin,Zhang Ziwei
With the help of iron oxide nanoparticles, electron spin resonance spectroscopy (ESR) was applied to immunoassay. Iron oxide nanoparticles were used as the ESR probe in order to achieve an amplification of the signal resulting from the large amount of Fe 3+ ion enclosed in each nanoparticle. Rabbit IgG was used as antigen to test this method. Polyclonal antibody of rabbit IgG was used as antibody to detect the antigen. Iron oxide nanoparticle with a diameter of either 10 or 30 nm was labeled to the antibody, and Fe 3+ in the nanoparticle was probed for ESR signal. The sepharose beads were used as solid phase to which rabbit IgG was conjugated. The nanoparticle-labeled antibody was first added in the sample containing antigen, and the antigen-conjugated sepharose beads were then added into the sample. The nanoparticle-labeled antibody bound to the antigen on sepharose beads was separated from the sample by centrifugation and measured. We found that the detection ranges of the antigen obtained with nanoparticles of different sizes were different because the amount of antibody on nanoparticles of 10 nm was about one order of magnitude higher than that on nanoparticles of 30 nm. When 10 nm nanoparticle was used as probe, the upper limit of detection was 40.00 μg mL −1 , and the analytical sensitivity was 1.81 μg mL −1 . When 30 nm nanoparticle was used, the upper limit of detection was 3.00 μg mL −1 , and the sensitivity was 0.014 and 0.13 μg mL −1 depending on the ratio of nanoparticle to antibody.
Schematic diagram of procedure and ESR spectra
研究领域:
分析化学
·
纳米技术
·
生物化学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Chinese Herbal Medicines
·
2020
·
IF 0
Zu-hua Wang,Xue-yan Sun,Jiao-jiao Zhang,Francesca Giampieri,Cheng-ju Jiang,Ting-ting Feng,Zhi-wei Wang,Rong-yi Chen,Maurizio Battino,Ying Zhou
Objective To study the anti-hypertrophic scar effect of the six-herb Chinese medicine composition (SCMC) ointment on the rabbit ear hypertrophic scar models. Methods The optimal formulation of SCMC ointment matrix was screened by the orthogonal designs and a series of evaluation tests. The SCMC ointment was prepared through emulsifying method. The rabbit ear hypertrophic scar models were established and used to investigate the anti-hypertrophic scar effect of SCMC ointment. Results Our results demonstrated that all the quality control indications of the SCMC ointment met the requirements. A nti-hypertrophic scar activity results showed that all the rabbit ear scar tissues appeared different degrees of shrink and fading, and took an unobvious but palpable shift from hard to soft texture with the low, middle and high concentration SCMC ointments treatments in vivo . Additionally, on 21st day the scar area and thickness in different concentrations of SCMC ointment groups were significantly reduced than control group, in a concentration-dependent manner. The immunohistochemical results also indicated that the SCMC ointment had good anti-hypertrophic scar properties and could inhibit hypertrophic scar formation. Conclusion The SCMC ointment could improve the blood circulation condition of hypertrophic scar tissues. Our research has demonstrated the Chinese medicine composition ointment with good anti-hypertrophic scar properties that could be used to treat hypertrophic scars. Meanwhile, it provides a theoretical basis for further clinical application.
研究领域:
药理学
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医学
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中医药学
✓ 已验证
DOI
危险品化学品经营许可证（带存储）