Foods
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2024
·Vol. 13
·期 17
·2756
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IF 4.7
Linlin He,Yuqian Yan,Gang Zhang,Yanna Zhao,Fa Zhao,Zhuang Ding,Zhengping Wang
Folates, a crucial B-group vitamin, serve as a significant functional food supplement. Nevertheless, considerable obstacles persist in improving folates stability in liquid products. In this study, folic acid (FA) and 5-methyltetrahydrofolate (MTFA), two approved sources of folates, were encapsulated with sodium caseinate (NaCas) to enhance their stability. The protective effect of NaCas on folate molecules was investigated using experimental and computational methods. Meanwhile, the influence of divalent calcium ion (Ca2+) on the properties of the NaCas-MTFA complex was examined to evaluate the potential application of calcium 5-methyltetrahydrofolate (CaMTFA). Fluorescence tests showed both folates had static quenching behavior and bound to NaCas with a binding constant of 104–105M−1. Hydrophobic interactions were crucial in NaCas-FA complex formation, while hydrogen bonding drove NaCas-MTFA binding. The encapsulation of caseinate notably slowed down the degradation of folates under both light and dark conditions. Moreover, the addition of a low concentration of Ca2+did not adversely impact the binding mechanism of the NaCas-MTFA complex or the degradation curve of MTFA. The results of this study could serve as a valuable resource for the utilization of caseinates in incorporating folates, specifically MTFA, in the creation of natural liquid dietary supplements.
研究领域:
食品科学
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营养学
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光谱学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2019
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IF 2.606
Wenhui Gao,Xiaoting Jia,Jinjun Wu,Ying Song,Jiang Yin,Meimei Zhang,Ni Qiu,Xiaoyan Li,Peng Wu,Xiaoxiao Qi,Zhongqiu Liu
Sorafenib is a multitarget tyrosine kinase inhibitor for the first-line treatment of hepatocellular carcinoma (HCC). However, moderate therapeutic effects are common in the clinic. Nanotechnology has provided feasible strategies to solve this problem. In the current study, we coated sorafenib with human serum albumin (HSA) and grafted folic acid (FA) onto the nanoparticle surface (FA-HSA-SRF-NPs) to enhance tumor targeting. Our results showed that the new nanoparticles had a high loading efficiency with a small diameter and good stability. In vitro , the FA-HSA-SRF-NPs showed enhanced cytotoxicity against hepatocellular BEL-7402 cells and increased the safety for normal liver LO2 cells. In vivo , FA-HSA-SRF-NPs exhibited effective antitumor activity toward nude mice bearing xenograft tumors without systemic toxicity, and this antitumor activity manifested as the inhibition of tumor proliferation and suppression of tumor angiogenesis. In addition, FA-HSA-SRF-NPs notably enhanced the accumulation of sorafenib in the tumor tissues of mice and markedly increased the amount of sorafenib in systemic circulation in rats. This current study indicated that FA-HSA-SRF-NPs could increase the efficacy of sorafenib against liver tumors that highly express the folate receptor (FR) and possess good safety, thereby highlighting their potential clinical value.
研究领域:
药学
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纳米技术
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肿瘤学
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产品关联
DOI
高影响因子
International Journal of Nanomedicine
·
2018
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IF 4.37
Yang Chen,Wei Liu,Yue Shang,Peipei Cao,Jianlin Cui,Zongjin Li,Xuebo Yin,Yuhao Li
Background Hepatocellular carcinoma (HCC) is the most common primary liver cancer and severely threatens human health. Since the prognosis of advanced HCC remains poor, there is an urgent need to develop new therapeutic approaches. Porphyrin metal-organic frameworks are a class of porous organic-inorganic hybrid functional materials with good biocompatibility. Methods Gadolinium-porphyrin metal-organic frameworks were used as a skeleton for folic acid (FA) conjugation to synthesize a novel type of nanoparticle, denoted as folic acid-nanoscale gadolinium-porphyrin metal-organic frameworks (FA-NPMOFs). The FA-NPMOFs were characterized using transmission electron microscopy, Fourier transform infrared spectroscopy and thermogravimetric-differential thermal analysis. The biotoxicity and imaging capability of the FA-NPMOFs were determined using HepG2 cells and embryonic and larval zebrafish. The delivery and photodynamic therapeutic effect of FA-NPMOFs were explored in transgenic zebrafish with doxycycline-induced HCC. Results FA-NPMOFs were spherical in structure with good dispersion and water solubility. They showed low biotoxicity, emitted bright red fluorescence, and exhibited an excellent magnetic resonance imaging capability, both in vitro and in vivo . Meanwhile, the FA-NPMOFs exhibited a strong affinity for folate receptor (FR)-expressing cells and were delivered to the tumor site in a targeted manner. Moreover, HCC tumor cells were eliminated following laser irradiation. Conclusion FA-NPMOFs can be used for dual-modality imaging and photodynamic therapy in HCC and show promise for use as a carrier in new therapies for HCC and other FR-positive tumors.
研究领域:
肿瘤学
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纳米技术
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生物医学工程
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药学
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产品关联
DOI
PubMed
高影响因子
ULTRASONICS SONOCHEMISTRY
·
2023
·
IF 8.4
Wei Fan,Hanyi Duan,Xiaolan Ren,Xiaoyan Guo,Yachao Zhang,Jisheng Li,Fengying Zhang,Juan Chen,Xue Yang
The mulberry leaf protein extracted by ultrasound-assisted cellulase degradation (UACD) method was optimized with the protein dissolution amount (PDA) as the index. The Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy of extracted mulberry leaf protein were measured. The functional characteristics of protein extracted by the UACD method were evaluated. Results showed that the extraction condition was optimized and adjusted to the following parameters: pH value of 7.20, ultrasound temperature of 35.00 °C, enzyme dosage of 4.20% and ultrasound time of 10.00 min. Under these optimized conditions, the experimental verification value of PDA was 13.87 mg/mL, which was approaching to the predicted value of 13.54 mg/mL. The analysis results of FTIR showed that after extraction by the UACD method, the mulberry leaf protein with the vibrational peak of ester carbonyl (C = O) absorption peak (1734.66 cm −1 ) disappeared. The α -helix content of protein extracted by the UACD decreased by 8.13%, and the β-turn and random coil content of protein increased by 20.22% and 18.79%, respectively, compared to that of the blank. The microstructure of mulberry leaf protein showed that the UACD method could break the dense structure of protein raw materials, reduce the average size of proteins and increase the specific surface area and roughness of proteins. According to the results of functional characteristics, the mulberry leaf protein extracted by the UACD method presented the highest enzymolysis properties and solubility, which was beneficial for the application in the food industry. In conclusion, the UACD method was a very effective way to extract protein from mulberry leaf.
研究领域:
食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL PHYSICS
·
2024
·
IF 2
Zhifeng Cai,Qi Liang,Yutong Wang,Xiao Wang,Lulu Jing,Yajie Cheng,Jinqi Liu,Zixuan Jia
Bright, blue fluorescent carbon quantum dots ( N -CDs) were carried out utilizing phenylenediamine and ascorbic acid as raw precursor through the hydrothermal method. The as-designed N -CDs suggested excitation-dependent characteristic changing from 340 nm to 380 nm. Moreover, Folic acid could availably quench the fluorescence intensity of the N -CDs. According to this experimental phenomenon, the sensitivity and selectivity of N -CDs for folic acid detection were developed. Under optimal experimental conditions, the as-prepared probe displayed excellent linearity with the folic acid concentration changing from 0.5 to 200 μM, and the limit of detection was 0.079 μM. Furthermore, the as-prepared probe indicated good sensitivity towards folic acid sensing in glucose and bovine serum samples. The recoveries of 95.9–104.8 % and 98.6–106.9 % were obtained in glucose and bovine serum samples, respectively. The corresponding relative standard deviation was lower than 2.74 % and 2.91 %, respectively. With preeminent sensitivity and selectivity, this as-developed nanosensor afforded a prospective platform for measuring folic acid in real samples. Furthermore, this N -CDs could be applied for the temperature detection.
研究领域:
分析化学
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生物医学工程
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纳米技术
✓ 已验证
产品关联
DOI
高影响因子
Foods
·
2024
·Vol. 13
·期 5
·635
·
IF 4.7
Aiping Li,Chen Wang,Zhenghao Wu,Yingying Liu,Zhenxia Hao,Chengyin Lu,Hongping Chen
Ningnanmycin is a widely used antibiotic in agricultural production that effectively controls fungal and viral diseases in tea trees and chrysanthemums. The polarity characteristic of ningnanmycin has posed limitations on the development of robust detection methods, thereby hindering effective monitoring and control measures. By combining cation exchange solid phase extraction (SPE) with hydrophilic interaction chromatography tandem mass spectrometry (HILIC-MS/MS), we have effectively tackled the issue pertaining to the separation and retention of ningnanmycin. The average recoveries of ningnanmycin in green tea, black tea, and chrysanthemum were 77.3–82.0%, 80.1–81.5%, and 74.0–80.0%, respectively. The intraday and interday relative standard deviations (RSDs) were below and equal to 7.7%. Good linearity was observed in the concentration range of 1–1000 μg/L (R2 > 0.998). The limits of detection (LODs) ranged from 1.1 μg/kg to 7.1 μg/kg, and the limits of quantification (LOQs) ranged from 3.6 μg/kg to 23.7 μg/kg for ningnanmycin. These results indicate the good accuracy, repeatability, reproducibility, and sensitivity of the method. It is suitable for detecting ningnanmycin in tea and chrysanthemum.
研究领域:
分析化学
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食品科学
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植物保护
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2021
·
IF 6.057
Shemsu Ligani Fereja,Ping Li,Jinhan Guo,Zhongying Fang,Ziwei Zhang,Zhihua Zhuang,Xiaohui Zhang,Kaifan Liu,Wei Chen
Folic acid (FA) is the natural form of water-soluble vitamins widely found in most plants and animal products and its deficiency leads to several human body abnormalities. The advancements of metal nanoclusters are highly increasing due to their molecule-like optical properties and attractive applications. Because of increasingly demand of noble metal nanoclusters as sensing templates, different synthesis methods have been developed for facile synthesis of noble metal nanoclusters . Herein, red-emitting fluorescent bovine serum albumin (BSA)-capped Au–Ag bimetallic NCs are facilely synthesized through green one-pot synthetic approach. The effect of silver on the fluorescence properties of Au NCs was investigated and it was found that introduction of silver can enhance the fluorescence intensity . The fluorescence intensity of the as-prepared Au–Ag nanoclusters gets quenched in the presence of folic acid in an aqueous medium and it was used as ultrasensitive sensing probe for FA detection. The developed Au–Ag NCs-based sensing probe shows linear response in the wide range of 0–100 μM and the detection limit is as low as 0.47 nM. Its applicability has also been confirmed successfully in real human serum, urine and FA tablet samples. Due to the high stability, sensitivity and selectivity , the developed bimetallic cluster sensing system is highly promising to be applied in the pharmaceutical and clinical laboratories.
研究领域:
生物化学
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分析化学
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医学诊断
✓ 已验证
DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2018
·
IF 3.974
Yong Li,Lixuan Yang,Huangqian Yan,Meng Zhang,Jing Ge,Xiangyang Yu
The uptake, translocation and accumulation behaviours of imidacloprid in six leafy vegetables , including Xiadi cabbage (XDC), Huaguan cabbage (HGC), Gaogengbai (GGB), Shanghaiqing (SHQ), Kangresijiqing (KRQ) and Ziyoucai (ZYC), were investigated under hydroponic conditions. Seedling stage (S-stage), rapid growth stage (R-stage) and maturation stage (M-stage) for each vegetable were considered. The root concentration factor (RCF), translocation factor (TF) and bioconcentration factor (BCF) were used to compare the difference. The results show that during 48 h of exposure, the total amount of imidacloprid taken up by the test vegetables increased with vegetable growth; however, the imidacloprid concentration in vegetable tissues varied significantly according to the variety and growth stage. For individual vegetables, imidacloprid was most easily accumulated into HGC and GGB shoots at R-stage, but into XDC, KRC and ZYC shoots at S-stage. For varieties, the ability of accumulating imidacloprid from solution to shoots seemed to follow the order of GGB > XDC > SHQ > KRQ > HGC > ZYC. Pearson's correlation analysis revealed significant negative correlations between daily transpiration and log BCF and between log RCF and log TF , indicating that the difference of uptake, translocation and accumulation behaviours of imidacloprid in vegetable varieties and growth stages may be related to the daily transpiration and the ability of concentrating imidacloprid in vegetable roots.
研究领域:
植物学
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环境科学
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DOI
PubMed
高影响因子
PLANT SCIENCE
·
2025
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IF 4.1
Ruilan Ran,Xiaofeng Li,Jiwei Zhang,Jiecai Zhao,Xin Zhao,Xiaoyun Cui,Guoxiong Chen,Pengshan Zhao
Sand rice ( Agriophyllum squarrosum (L.) Moq.), a species of Amaranthaceae sensu lato , is an annual psammophyte found mainly on sand dunes in arid and semi-arid regions of Central Asia. Trichomes on the leaf surface are a key adaptive feature of sand rice, however, their ecophysiological functions remain to be elucidated. In this study, the anatomical analysis of a leaf reveals that the foliage is characterized by its isobilateral and amphistomatic nature. Together with an average δ 13 C value of −28.57 ‰, these findings indicate that sand rice is a C 3 plant. Utilizing a trichome-defective mutant ( Agriophyllum squarrosum trichomeless1 , astcl1 ) identified from an EMS mutagenesis library by our group, alongside the wild type Shapotou (SPT), we observed that both epidermal surfaces on SPT leaves exhibit dense and dendritic trichomes, forming rough leaf boundary layers. Chlorophyll leaching analysis revealed that SPT has a higher epidermal permeability, with 86.32 % of chlorophylls leaching out within 30 minutes, compared to only 24.18 % for astcl1 . The astcl1 leaf displayed a higher leaf wettability, lower water loss, and thicker cuticle compared to SPT leaves, which may be associated with trichomes serving as the primary pathway for nonstomatal water movement. This is supported by the observation that toluidine blue staining is more intense in trichomes than in pavement cells. Our findings reveal that the correlation between trichomes and the cuticle appears to be a critical factor in managing water loss and overall plant hydration, highlighting the significant role of trichomes in the adaptation of sand rice to desert environments.
研究领域:
植物学
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生态学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2024
·Vol. 25
·期 5
·2701
·
IF 4.9
Shizi Zhang,Yunfeng Xu,Fang Wang,Liyun Yang,Lijuan Luo,Lingyan Jiang
Anthracnose caused byColletotrichum gloeosporioidesis a destructive disease ofStylosanthes(stylo). Combination treatment of phloretin and pterostilbene (PP) has been previously shown to effectively inhibit the conidial germination and mycelial growth ofC. gloeosporioidesin vitro. In this study, the effects of PP treatment on the growth ofC. gloeosporioidesin vivo and the biocontrol mechanisms were investigated. We found that exogenous PP treatment could limit the growth ofC. gloeosporioidesand alleviate the damage of anthracnose in stylo. Comparative transcriptome analysis revealed that 565 genes were up-regulated and 239 genes were down-regulated upon PP treatment during the infection byC. gloeosporioides. The differentially expressed genes were mainly related to oxidative stress and chloroplast organization. Further physiological analysis revealed that application of PP afterC. gloeosporioidesinoculation significantly reduced the accumulation of O2•−level and increased the accumulation of antioxidants (glutathione, ascorbic acid and flavonoids) as well as the enzyme activity of total antioxidant capacity, superoxide dismutase, catalase, glutathione reductase, peroxidase and ascorbate peroxidase. PP also reduced the decline of chlorophyll a + b and increased the content of carotenoid in response toC. gloeosporioidesinfection. These results suggest that PP treatment alleviates anthracnose by improving antioxidant capacity and reducing the damage of chloroplasts, providing insights into the biocontrol mechanisms of PP on the stylo against anthracnose.Keywords:phloretin;pterostilbene;ROS scavenging;chloroplasts;anthracnose;stylo
研究领域:
植物病理学
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分子生物学
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植物生理学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Aiping Li,Chen Wang,Yunfeng Chai,Zhiming Huang,Binbin Yao,Chengyin Lu,Miao Yu,Hongping Chen
Validamycin A (VA) and spinosyn A (SA) have been utilized as antibiotic pesticides in tea plantations for the control of pests and diseases. Both pesticides pose significant risks to human health and have been listed as key controlled new pollutants in China. So far, there have been few reports for the risk assessment of antibiotic pesticides in tea. In this study, a hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry (HILIC-MS/MS) method was developed for the simultaneous detection of VA and SA residues in tea. Method validation demonstrated good accuracy and precision. During tea planting, SA and VA in tea shoots degraded fast, with the half-life of 0.36 to 0.43 days for SA and 0.72 to 0.92 days for VA. SA and VA in tea shoots degraded fast initially and then followed by a slower phase. Both SA and VA degraded rapidly during the withering and drying stages. Compared to green tea, the degradation rates of both pesticides were faster during black tea manufacturing. Transfer rates of VA and SA from tea into infusions had transfer rates of 76.7 % to 99.5 % and 1.5 % to 2.7 %, respectively. The chronic risk quotient (RQc) < 1 for both antibiotic pesticides indicated negligible consumer risk from tea.
研究领域:
农业科学
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食品科学
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环境科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2024
·
IF 4.1
Bowen Zha,Xinhui Huang,Liangbin Lin,Hongyu Guo,Fafu Yang
Although some detection methods for folic acid have been developed based on the conventional instruments, the rapid, simple and in-situ detection for folic acid was rarely reported. In this work, a “turn-on” and “color-changing” fluorescence sensor ( TBCS ) on detecting folic acid was designed and synthesized. TBCS emits the green fluorescence, which transferred to the strong blue fluorescence after binding folic acid. The high sensing selectivity was observed among various ions and biomolecules. The detection limitation of TBCS for folic acid was 4.32 × 10 −7 M. The sensing mechanism was proposed as that the ICT effectiveness of TBCS was interrupted by sensing folic acid. The application experiments on test paper, folic acid tablets and simulated sample displayed the good in-situ detecting ability of TBCS for folic acid, suggesting the good application prospect on rapid, simple and in-situ detecting folic acid in real environments.
研究领域:
分析化学
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生物医学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）