MICROCHIMICA ACTA
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2025
·Vol. 192
·期 7
·1-9
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IF 5.3
Yao Zimin,Zheng Guoliang,Zheng Yonggui,Wen Xiaokun,Hong Xia,Yan Jiayi,Liu Wen,Wang Jiwei,Wang Kexin,Cao Shuo
Biomolecular detection based on a single fluorescence signal response is often difficult to completely eliminate the influence from interfering factors on the assay results. In this work, a ratiometric fluorescence probe was designed based on a dual emission carbon dot (CD). It produces dual-color emission (400 nm blue emission band and 560 nm green emission band) under 360 nm excitation. The two emission bands are believed to be originated from the distinct luminescent mechanisms: the intrinsic emission of CDs and intramolecular charge transfer (ICT) process, respectively. The energy transfer between the dual emission leads to the differentiated responses towards sialic acid (SA). As the concentration of SA increases, the two emission bands of CDs dispersion exhibit opposite variation trends. The blue emission intensity rose slightly and green emission intensity descended. The ratios of emission band intensity at 400 nm and 560 nm are served as quantitative assay of SA. The limit of detection reached 1.15 μM. The ratiometric fluorescence probe also shows a favorable selectivity for SA detection. The self-calibration and mutual verification functions of the probes enable consistent emission ratios across different models of commercial spectrometers. It minimizes the interference from the environment and instrumentation, achieving accurate and highly versatile SA detection. The utility of the probe for SA detection was verified by analyzing human serum, showing the tremendous potential of dual emission CDs for accurate SA detection. Graphical
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JOURNAL OF MOLECULAR STRUCTURE
·
2025
·
IF 4.7
Xiaoyu Shen,Yejian Mo,Jian Ren,Kexin Liu,Chunlin Deng
Hyperuricemia (HUA) is a metabolic disease caused by elevated uric acid levels, with xanthine oxidase (XOD) as the key enzyme in uric acid production. Current treatments primarily use XOD inhibitors (e.g., allopurinol and febuxostat), but their lack of antioxidant activity limits their ability to counteract oxidative stress in HUA. Gallic acid (GA), a polyphenol with antioxidant properties, weakly inhibits XOD but is insufficient alone. Transition metal ions like Cu²⁺ are potent reversible XOD inhibitors, yet excessive concentrations cause toxicity. The coordination reaction effectively reduces metal ion toxicity while partially preserving the inherent antioxidant properties of polyphenols. Using gallic acid (GA) as a ligand with Cu²⁺, we synthesized Cu-GA complex, which forms a stable coordination polymer that minimally releases free Cu²⁺ in water. Cu-GA demonstrated superior XOD inhibition compared to allopurinol (AP), GA, and Cu²⁺ alone, functioning as a reversible inhibitor to reduce uric acid levels. Additionally, it maintained significant antioxidant activity while showing substantially lower cytotoxicity in LO2 and AML12 cells than free Cu²⁺. In conclusion, for Cu-GA, the coordination reaction retained the inhibitory effect of Cu 2+ on XOD, while conferring some antioxidant effects, and also reduced the biotoxicity of Cu 2+ .
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BIOSENSORS & BIOELECTRONICS
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2026
·
IF 11.8
Lingsong Lu,Yingying Zhang,Bei Liu,Nuo zhou,Dao jun Yu,Ying Wang
Circular RNAs (circRNAs) represent an emerging family of noncoding transcripts defined by closed-loop architecture, which are now established as key participants in the etiology of tumorigenesis and malignant progression. While circRNAs show potential as therapeutic targets and biomarkers, the accurate detection of circRNAs remains challenging due to interference from homologous linear RNAs. In this study, an ultrasensitive method for detecting circ_0051240 based on the sulfhydrated toehold capture probe-initiator assembly-mediated hybridization chain reaction (HCR) and the CRISPR-Cas12a system was developed. The capture probe (with a toehold domain)-initiator strand duplex was ingeniously fabricated to identify the back-splice junction (BSJ) of circRNA. After magnetic enrichment and separation by the Fe 3 O 4 -Au@UIO-66-NH 2 nanocomposite, the initiator strand (H0) was delivered to trigger HCR. The HCR product dsDNA concatemers contained multiply repeated CRISPR-targetable DNA sites that were readily recognized by the CRISPR RNA (crRNA). This specific recognition and binding activated the CRISPR-Cas12a system's collateral endonuclease activity, leading to cleavage of the fluorophore-quencher (FQ) reporters and fluorescence emission at a characteristic wavelength. This design eliminated linear RNA-related interference and enhanced the detection of fluorescence intensity (FI). Under optimal conditions, the proposed HCR/CRISPR-Cas12a method exhibited a wide quantitative measurement range spanning from 45 pM up to 180 nM, achieving a notable limit of detection (LOD) of 0.03 pM. In this study, a novel circRNA sensing strategy capable of accurate and highly sensitive quantification of ovarian cancer-specific circRNA was reported. The proposed method exhibits acceptable performance when compared to present approaches.
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JOURNAL OF FLUORESCENCE
·
2026
·
IF 3.4
Su Qian,Huang Li,Zhang Youjin,Yang Zhiyong
Carbon dots (CDs) have been broadly employed in many fields due to their excellent fluorescence property, especially in pharmaceutical analysis. Herein, we developed a simple and rapid method to prepare the phosphorus-nitrogen (P, N) co-doped carbon dots via a 3-minute 50-second microwave-assisted synthesis, using N-butyldiethanolamine as the carbon source with phosphoric acid accelerating the CDs formation reaction. The prepared CDs (termed CD1) were characterized by TEM, FT-IR, XPS, XRD and fluorescence spectroscopy. P, N co-doped CDs exhibited notable water solubility, a quantum yield of 19.7% and good photobleaching resistance. Taking advantage of their superior fluorescence, these CDs served as a bright-blue probe for tigecycline detection through fluorescence quenching. The sensor displayed excellent selectivity and anti-interference ability against common coexisting drugs (e.g., voriconazole, ceftazidime, amikacin). Under optimized conditions, a wide linear range (1-936 µM) between F 0 / F and Tigecycline concentration was established, with a correlation coefficient (R) of 0.984, and the detection limit (LOD) was calculated to be 1.10 µM. Furthermore, we clarified the fluorescence quenching mechanism of CD1 toward tigecycline, which was attributed to the internal filtration effect (IFE). Thus, this work demonstrated a novel P, N co-doped CD-based sensing strategy for rapid tigecycline monitoring.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2026
·
IF 8.3
Lin Wang,Xinbao Zhang,Yuxi Jiao,Mengqiong Zhang,Xinyan Liu,Xiujie Li,Xiangxue Zhu,Shuang Han,Haijun Zhang,Jiping Chen,Lidong Wu,Xianbo Lu
Discriminating and selective detection of structurally similar multi-analytes remains a major challenge in chemical sensing due to severe cross-response interference from shared molecular skeletons and functional groups. To address this, we report a novel zeolite-based nanopore sensing strategy that leverages the uniform nanopore channels and tunable adsorption properties of zeolites for achieving high selectivity in multi-analyte systems. Using bisphenol AF (BPAF) and bisphenol S (BPS)—two electrochemically overlapping phenolic pollutants with extreme similar structure—as model targets, we engineered and studied four zeolites: hydrophilic Hβ, surface-hydrophobic OS-Hβ (prepared by organosilane modification of Hβ), and two hydrophilic Y-type zeolites with contrasting Si/Al ratios (HY‑686 and NaY‑5). Notably, these materials exhibited distinct nanopore adsorption preferences: Hβ selectively adsorbed BPS, whereas OS-Hβ favored BPAF only; HY‑686 adsorbed both bisphenols, while NaY‑5 showed negligible uptake. Mechanistic studies revealed that for Beta-type zeolites with similar nanopore sizes, surface hydrophilicity/hydrophobicity governs selectivity, whereas for hydrophilic Y-type zeolites, nanopore size plays the decisive role. Building on this principle, we developed an innovative electrochemical nanopore sensing platform that integrates selective molecular absorption, in situ electrocatalysis, and interference-free detection, enabling accurate discrimination and quantification of BPAF and BPS in complex samples. This work not only resolves a persistent sensing challenge for structurally analogous analytes but also establishes a generalizable molecular‑sieving nanopore sensing principle for selective adsorption, electrocatalysis, and sensing applications.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2026
·
IF 8.3
Mingpeng Yang,Chengyu Ruan,Jun Cai,Liya Ge,Grzegorz Lisak
Sweat glucose monitoring has emerged as a promising non-invasive approach for real-time tracking of glucose dynamics, with potential applications in diabetes management and exercise-related health analytics. However, the practical deployment of wearable sweat glucose sensors remains challenged by efficient sweat collection, reliable measurement under physiological variability, sustainable power supply, and user comfort. In this study, a fully integrated wearable patch tailored for practical applications is introduced, enabling the real-time collection and measurement of glucose from micro-scale sweat. The patch integrates a paper-based microfluidic chip, an enzymatic electrochemical glucose sensor, and a Near-field communication (NFC)-enabled wireless power and data transmission module into a compact platform (5.6 cm × 3 cm). Glucose analysis can be achieved using only 25 μL of sweat within 28 s, while NFC provides a stable operating voltage of 2.9 V and real-time communication with a smartphone. The glucose sensing electrode is fabricated by electrodepositing a Prussian blue (PB)-doped poly(3,4-ethylenedioxythiophene) (PEDOT) nanocomposite, achieving a sensitivity of 6.9 nA μM −1 and a linear detection range of 1 – 1300 μM, with reliable interference resistance and stability. Preliminary on-body tests show a positive correlation between sweat glucose data from the patch and blood glucose readings from a commercial glucose meter, indicating a similar dynamic trend. By emphasizing system-level integration, battery-free operation, modular architecture, and user-friendly deployment, this work provides an application-oriented wearable platform that facilitates the translation of sweat glucose sensing from laboratory prototypes toward practical continuous monitoring devices for diabetes management and personalized health analytics.
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ELECTROCHIMICA ACTA
·
2026
·
IF 5.8
Qiao Wan,Yajun Li,Yahong Su,Wenqing Jia,Jiang Zhu,Qiang Yu,Zhen Chen,Lingli Lei,Yuanyuan Zhang
This study aims to develop a high-performance, low-cost non-enzymatic electrochemical sensing materials for glucose detection, addressing the limitations of existing technologies. A three-dimensional porous hierarchical layered-nanoflower heterostructured CuCoP/MXene composite was synthesized through in situ growth of CuCo-MOF nanoflowers on Ti 3 C 2 -MXene via a one-step hydrothermal method, followed by oxidation and phosphidation treatments. This unique architecture significantly increases the specific reactive surface area, exposes abundant active sites, and provides sufficient interfacial regions for glucose oxidation. The synergistic effect between copper (Cu) and cobalt (Co) further enhances the electrocatalytic performance. Meanwhile, MXene serves as a conductive scaffold that not only facilitates electron transfer but also promotes the diffusion and adsorption of glucose molecules, owing to its excellent conductivity and continuous interlayer channels. The fabricated sensor exhibits an extensive linear detection range (0.5-6000 μM), high sensitivity (1408.49 μA mM -1 ·cm -2 ), a low detection limit (0.14 μM, S/N=3), along with excellent selectivity, reproducibility, and stability, together with a swift response time (3 s). Moreover, the sensor has been successfully applied to the accurate detection of glucosein samples of serum, showing high reliability and promising practical value.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2026
·
IF 3.8
Xia Yong,Wang Qi,Wang Xu,Shan Liancong,Zhang Bo,Li Sheng,Guo Chang
The accurate and rapid detection of fluoroquinolone antibiotics (FQs) is crucial, yet their structural homology often leads to cross-reactivity that compromise accuracy. Herein, we designed a poly(3,4-ethylenedioxythiophene) doped with a poly(styrenesulfonate) (PEDOT:PSS)-based dual-channel organic electrochemical transistor (OECT) array on a flexible, low-cost polyimide substrate. By leveraging the synergistic catalysis of single-walled carbon nanotubes (SWCNTs) and gold nanoparticles (AuNPs), effective discrimination of three FQs was achieved using gate electrodes modified with only two Nafion/SWCNTs/AuNPs composites, differing solely in AuNP mass fraction—this significantly simplifies fabrication for an electronic tongue system (ETS). Aided by principal component analysis (PCA), the system discriminates and quantifies ofloxacin (OFL), levofloxacin (LEV), and ciprofloxacin (CIP) across a wide concentration range of 0.4–1000 μM, with limits of detection (LODs) of 0.32–0.40 μM, while exhibiting excellent selectivity, anti-interference, consistency, stability, and rapid response. The comprehensive performance of this platform establishes a practical foundation for portable FQ sensing in environmental and clinical applications. Graphical abstract
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DYES AND PIGMENTS
·
2026
·
IF 3.9
Shen Zhang,Zhuo Wang
In consideration of the potential threat, the establishment of efficient approach for fluazinam determination is necessary. Based on the superior property of carbon dots and biomass, sulfur doped biomass-based carbon dots (SBCDs) were constructed using a simple hydrothermal approach, in which peanuts were applied as carbon sources with the addition of methionine. The well-dispersed SBCDs displayed optimal emission wavelength of 435 nm under the excitation wavelength of 364 nm. Owing to the detection principle of fluorescence quenching through static quenching and inner filter effects, the SBCDs were used to detect fluazinam with a broad linear range of 0-100 μM and a detection limit of 0.026 μM. Additionally, the SBCDs also exhibited temperature sensing with a wide linear range of 15-95 °C. Moreover, the as-prepared SBCDs were effectively used for the determination of fluazinam in tap water and orange juice samples. The SBCDs displayed tremendous potential for the sensing of fluazinam and temperature.
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JOURNAL OF ALLOYS AND COMPOUNDS
·
2026
·
IF 6.7
Xiaoqian Liu,Linghui Hao,Caiqing Song,Qiaoling Shen,Meirou Jing,Jiaying Xu,Lijun Bian,Lijie Sun
Dopamine (DA) and uric acid (UA) are important biomarkers for various diseases, and the development of reliable detection platforms for their detection is of significant value. In this study, nickel manganese oxide (NMO) was synthesized via a hydrothermal method, followed by thermal treatment. The morphology, structure, and composition of the samples were systematically characterized using scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray photoelectron spectroscopy (XPS). To enhance the electrical conductivity of the material, NMO was composited with carboxylated carbon nanotubes (CCNT) to form an NMO/CCNT composite, which was then used for the simultaneous detection of DA and UA. Among the synthesized series of materials, the sample with a nickel-to-manganese molar ratio of 1:2 (denoted as N 1 M 2 O) exhibited the best sensing performance, which was attributed to its unique bimetallic synergistic effect. In the presence of ascorbic acid, the N 1 M 2 O/CCNT electrode showed a good linear response to both DA and UA within the concentration range of 1–15 μM. Furthermore, the sensor achieved satisfactory recovery rates of 96.5–105.2 % in fetal bovine serum, demonstrating its potential for practical application.
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2026
·
IF 4.1
Liuyi Huang,Xinhui Gu,Fengna Xi
Establishing sensitive detection method for carbohydrate antigen 15–3 (CA15–3) is of great significance for the early diagnosis and therapeutic monitoring of breast cancer. Herein, an electrochemiluminescence (ECL) sensor was fabricated by growing Co 3 O 4 nanoparticles in-situ within nanochannels and employing a signal gating strategy, achieving highly sensitive detection of CA15–3. Using simple Stöber method, a silica nanochannel film (SNF) was deposited onto an indium tin oxide (ITO) glass electrode. This two-dimensional (2D) nanoporous silica film provided a confined space, within which Co 3 O 4 nanozymes were synthesized in situ. Co 3 O 4 served as a peroxidase (POD)-mimic nanozyme, enabling the catalytic conversion of hydrogen peroxide (H 2 O 2 ) into reactive oxygen species (ROS). Functioning as a nanocatalyst, Co 3 O 4 remarkedly enhanced the electrochemical oxidation of luminol, thereby boosting the ECL signal. This dual enhancement mechanism enabled the sensor to achieve high luminol-based ECL signal. The bio-recognition interface was constructed by functionalizing the outer surface of SNF with silane coupling agents and immobilizing aptamers. A measurable decrease in the ECL signal was generated due to the hindered diffusion of the emitter and co-reactant and increased interfacial resistance, which occurred when the aptamer-CA15–3 complex formed on the electrode surface. This response allowed for the quantification of CA15–3. Under optimal conditions, the developed aptasensor can detect CA15–3 in the range of 0.1 to 10,000 mU/mL, with a limit of detection of 26 μU/mL. Owing to its merits of simple fabrication, rapid analysis, high sensitivity, good reproducibility, and excellent selectivity, the developed immunosensor holds great potential for clinical diagnostics of tumor biomarkers.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2026
·
IF 9.7
Ziyue Ju,Ruichan Lv,Ji Zhang,Delong Shi,Yitong Chen,Anees A. Ansari
Conventional techniques such as screen printing and hand-brushing, though scalable, often compromise on patterning resolution and material waste. In contrast, advanced inkjet printing offers a promising route for high-resolution and efficient manufacturing. In this study, a flexible electrode based on Ni-MOF material used high-precision scalable inkjet printing was developed for glucose sensing in human body fluids for wearable devices. Initially, Ni-MOF material with a high specific surface area and porous structure was synthesized via a hydrothermal method, and its potential for electrochemical detection was investigated. Experimental results demonstrated that the material exhibited high sensitivity and a broad detection range for glucose. Importantly, graphite and carbon nanotubes were incorporated to construct a Ni-MOF composite material with the composition ratio optimized to enhance conductivity and mechanical stability, for further application in flexible electrodes. Typically, a cost-effective, high-efficiency, and scalable fabrication process was achieved through high-precision inkjet printing technology, which enables 1200 dpi resolution while supporting ultra-large-scale printing up to 1200 × 1200 mm. Simutaneously, simulation-based optimization was conducted to determine the most effective electrode shape. As a result, the detection range of the inkjet-printed flexible electrode was extended to 0.005–2.63 mM, with an improved sensitivity of 12.2 μA·μM −1 ·cm −2 . Moreover, excellent mechanical stability and long-term durability were achieved, enabling precise differentiation of glucose fluctuations in low-concentration simulated sweat. Finally, a microfluidic channel to provide environment for the reaction was designed, thereby establishing a fundamental foundation for applying the flexible electrode in wearable devices.
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