Quantifying the binding kinetics and affinities of protein–small molecule interactions is critical for biomarker validation, drug discovery, and deep understanding of various biological processes at the molecular-scale. Novel approaches are demanded as most common label-free techniques are mass-sensitive, which are not suitable for the detection of small molecule interactions. Here, an optical imaging platform is developed to measure the binding kinetics of both protein–small molecules and protein–ions based on monolayer MoS 2 , an ultra-thin 2D material whose optical absorption is extremely sensitive to charge. A model is established to calibrate the optical response due to the charged analyte binding and it is applied to quantify the interactions between abl1 kinase and different small-molecule inhibitors. Such a presented method is capable of distinguishing different inhibitors binding to a wild or mutated kinase, which provides guidance for drug evaluation and drug mechanism exploration. The binding kinetics of calcium ions to calmodulin is also measured, further broadening the application field of the method. In addition, the imaging capability allows mapping the local binding kinetics of the molecular interactions with a high resolution, which reveals visible spatial variability and offers a promising tool for studying heterogeneous local interfacial interactions.
Yang Bai,Yanbiao Zhao,Weidong Li,Linghui Meng,Yongping Bai,Guorong Chen
The developing sulfide solid electrolyte is a promising way to overcome shuttle effect of polysulfide in the traditional liquid Li-S battery. However, an air stable sulfide solid electrolyte and competitive solid–solid interface structure must be constructed firstly. Herein, we have designed an organic–inorganic multi-scale composite electrolytes to overcome the interfacial issues of solid state Li-S batteries (SSLSBs). The organic–inorganic composite electrolyte consists of a gel polymer electrolyte (GPE) and an oxygen doped inorganic sulfide solid electrolyte. The O doped sulfide solid electrolyte improves its chemical stability and suppresses the lithium dendrites. A thin GPE film protects the crystal structural stability during cycles and greatly enhances the performance of O doped sulfide solid electrolyte due to the GPE not only constructs soft interface contact between S cathode and solid electrolyte, but also builds lithium ion transport paths by ionic liquid between them. Those SSLSBs display a very good cycling performance with the specific capacity of 1023 mAh/g after 50 cycles at 60 °C and 541 mAh/g after 150 cycles at 30 °C under same rate of 0.1 C. This study demonstrates that the development of advanced solid-state Li-S battery needs to fully integrate a number of technical advantages.
Jingwei Gu,Ying Zeng,Xiaofang Feng,Ximin Wu,Chunmei Zeng,Mingqi Li
Nanosilicon@nonstoichiometric silicon oxide where nanosilicon is embedded in SiO x matrix (abbreviated as nano-Si@SiOx), is a very attractive material structure to suppress the volume effect of silicon in Lithium-ion batteries. In this work, we demonstrate that the combination of electrolysis and peeling technique produce nano-Si@SiOx from bulk SiO 2 . X-ray diffraction (XRD), X-ray photoelectron spectra (XPS), scanning electron microscope (SEM) and high resolution transmission electron microscope (HRTEM) are employed to characterize the prepared materials. The average O/Si atomic ratio of the synthesized nano-Si@SiOx is about 0.91. The nano-Si@SiOx/graphite composite prepared using it as the precursor exhibits a stable discharge capacity of about 1560 mAh g −1 (calculated on the total mass of the composite) with good cyclic stability and rate capability. This work provides a new routine to prepare nano-Si@SiOx composite.
危险品化学品经营许可证（带存储）