Algal Research-Biomass Biofuels and Bioproducts
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2025
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IF 4.5
Yongqi Tian,Ding Deng,Haitao Luo,Mingfeng He,Shaoyun Wang
Phlorotannins, recognized for their efficacy and safety as natural tyrosinase in hibitors, have posed challenges due to their limited extraction efficiency and the elusive nature of their mechanism of action. Here, we developed an ultrasonic-assisted enzymatic extraction (UAEE) protocol that significantly enhanced phlorotannin recovery from Sargassum horneri, achieving a total phenolic content (TPC) of 21.51 mg PGE/g DW. Subsequent purification using solvent extraction and Sephadex LH-20 yielded high-purity alginate phlorotannins (SHP), exhibiting potent DPPH radical scavenging and tyrosinase inhibitory activities, with IC 50 values of 22.71 and 26.90 μg/mL, respectively. Structural elucidation based on NMR and MS data suggested that SHP predominantly comprises fucophloroethols, with a degree of polymerization ranging from 3 to 8. Reciprocal plot analysis indicated that SHP inhibits tyrosinase activity through a competitive inhibition mechanism, while fluorescence spectroscopy revealed a dynamic quenching effect of SHP on tyrosinase. Molecular docking studies further elucidated t hat van der Waals forces and hydrophobic interactions are the primary forces governing the interaction between SHP and tyrosinase. Cellular experiments demonstrated the significant inhibitory effects of SHP on both melanin production and tyrosinase activity in B16-F10 cells. This research presents promising lead compounds for the development of novel tyrosinase inhibitors, while also providing theoretical and methodological foundations for the comprehensive utilization of Sargassum horneri.
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高影响因子
ACS Applied Materials & Interfaces
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2025
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IF 8.2
Xuanlong He,Xing Liu,Guomin Li,Qiuting Chen,Longjun He,Dingtao Ma,Yanyi Wang,Lipeng Zhang,Peixin Zhang
Aqueous zinc-ion batteries (AZIBs) are considered among the most promising energy storage systems because of their low cost, high safety, and high theoretical capacity. However, challenges including Zn2+ desolvation, side reactions, and dendritic growth significantly hinder their rate capability and cycling stability. In this study, we propose a molecule-substitution-induced anchoring strategy by introducing kojic acid molecules with carbon-based (C═O) and hydroxyl (C–OH) groups to modulate the Zn2+ solvation structure, lower the desolvation activation energy, and effectively suppress undesirable side reactions. More importantly, its pyranone ring (C–O–C) can anchor onto the Zn surface to form an adsorption layer, which induces the uniform deposition of Zn2+ ions and consequently suppresses dendrite formation. At a current density of 1 mA cm–2 and an areal capacity of 1 mAh cm–2, the symmetric cell demonstrated an ultralong lifespan of 2800 h and a high Coulombic efficiency of 99.72%. Furthermore, the assembled Zn||δ-MnO2 full cell had a capacity retention of 95% after 1500 cycles at 3 A g–1. Even at an elevated temperature of 50 °C, the cell retained a capacity of 197.9 mAh g–1 after 200 cycles.
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PubMed
高影响因子
Current Research in Food Science
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2025
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IF 7
Min Lv,Longkang Cao,Yulu Ding,Shan Gao,Yinxin Wu,Rong Li,Wenjian Tang,Lili Zhu
Novel kojic acid-tetrahydroisoquinoline hybrids ( 4a–4p ) were designed and synthesized to address the need for safe tyrosinase inhibitors in food preservation. Compound 4c emerged as the most potent inhibitor, exhibiting 40-fold greater activity (IC 50 = 1.27 μM) than kojic acid. Kinetic studies identified 4c as a mixed-type inhibitor ( K i = 1.48 μM, K is = 15.35 μM), with molecular docking revealing strong binding to tyrosinase's active site via copper chelation and hydrophobic interactions. Safety evaluations in zebrafish embryos and mammalian cell lines confirmed minimal cytotoxicity. Furthermore, 4c showed broad-spectrum antimicrobial activity against E. coli , S. aureus and B. cinerea . In practical applications, 4c effectively suppressed enzymatic browning in fresh-cut mushrooms. These results underscore 4c as a promising candidate for multifunctional food preservatives, combining anti-browning efficacy, microbial inhibition, and biocompatibility. This study provides a strategic framework for developing hybrid inhibitors to enhance food quality and safety.
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FITOTERAPIA
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2024
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IF 2.5
Yuan Wei,Wen-Hua Dong,Wei Li,Jun Zeng,Hui-Qin Chen,Sheng-Zhuo Huang,Li Yang,Wen-Li Mei,Ya-Li Wang,Zhi-Yong Guo,Hao-Fu Dai,Hao Wang
Six new dimeric 2-(2-phenylethyl)chromones ( 1 – 6 ) were successfully isolated from the ethanol extract of agarwood of Aquilaria filaria from Philippines under HPLC-MS guidance. Compounds 1 – 6 are all dimers formed by linking 5,6,7,8-tetrahydro-2-(2-phenylethyl)chromone and flindersia 2-(2-phenylethyl)chromone via a single ether bond, and the linkage site (C5-O-C8′′) of compound 2 is extremely rare. A variety of spectroscopic methods were used to ascertain their structures, including extensive 1D and 2D NMR spectroscopic analysis, HRESIMS, and comparison with literature. The in vitro tyrosinase inhibitory and anti-inflammatory activities of each isolate were assessed. Among these compounds, compound 2 had a tyrosinase inhibition effect with an IC 50 value of 27.71 ± 2.60 μM, and compound 4 exhibited moderate inhibition of nitric oxide production in lipopolysaccharide-stimulated RAW264.7 cells with an IC 50 value of 35.40 ± 1.04 μM.
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ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
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2025
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IF 3
Yilu Sun,Jia Zhao,Jianhui Rong
Skin hyperpigmentation due to UV-induced tyrosinase activation and melanin overproduction is an ongoing challenge in cosmetic and dermatological applications. While resveratrol analogues show anti-melanogenic potential, the structure-activity relationships of their glycosylated derivatives remain underexplored. Here, we investigate how the vinyl moiety in the food-derived stilbenoid glycoside piceid (resveratrol-3- O -β-glucoside) affects tyrosinase inhibition. We reduced the vinyl moiety to yield dihydropiceid by catalytic hydrogenation and systematically assessed both compounds for anti-melanogenic effects. As results, piceid exhibited superior monophenolase inhibition over dihydropiceid in enzyme kinetics, while both compounds showed comparable diphenolase inhibition. Cellular assays revealed that piceid reduced melanin production by 59.2 % at 25 μM in α-MSH-stimulated B16F10 melanoma cells, whereas dihydropiceid showed weaker activity (<25 % reduction). MolgpKa analysis indicated that the vinyl moiety lowered the 4′-OH pKa (9.7 vs. 9.9), while UV–vis spectroscopy validated that the vinyl moiety enhanced the copper chelation capacity of piceid (ΔOD: 0.459) over dihydropiceid (ΔOD: 0.233). Molecular docking revealed that 4′-OH in piceid closely coordinated the tyrosinase binuclear copper center, whereas molecular dynamics simulation validated that hydrogen bonding supports the binding of both compounds to tyrosinase. Collectively, this study establishes the vinyl moiety in dietary stilbenoids as a critical pharmacophore for tyrosinase inhibition and thereby provides a molecular basis for developing natural anti-hyperpigmentation functional foods or cosmeceuticals.
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