INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Chenggang Song,Wuliang Shi,Jian Yang,Gang Ding,Wei Chen,Chengguo Jia,Mingzhe Zhang,Jianchun Qin
The presence of aflatoxins resulting from Aspergillus flavus infection in food poses a significant threat to human health due to their potent carcinogenicity or hepatotoxicity. Monarda didyma essential oil, with 92.3% thymol as the active ingredient, is effective in inhibiting A. flavus infestation during peanut storage. Thymol was found to inhibit the growth of A. flavus with an IC 50 value of 14.5 µg mL −1 . When exposed to thymol stress, A. flavus experiences disruption of its glycolysis and tricarboxylic acid cycle, leading to the accumulation of reactive oxygen species and resulting in cell membrane damage and apoptosis. Furthermore, thymol leads to the downregulation of multiple genes, including aflA , involved in the aflatoxin biosynthesis, resulting in a reduction in the production of aflatoxins. This demonstrates the potential of M. didyma essential oil and thymol as effective inhibitors of mycotoxins, thereby contributing to the resolution of food safety issues.
研究领域:
食品科学
·
微生物学
·
药理学
·
真菌学
·
植物化学
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产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2020
·
IF 4.192
Zhongyu Li,Yan Mao,Jianwen Teng,Ning Xia,Li Huang,Baoyao Wei,Qingjin Chen
Citrinin (CIT), a known nephrotoxic mycotoxin, is mainly produced by Penicillium, Aspergillus, and Monascus species. It is a natural contaminant in cereal grains, foods, and feedstuff. Liupao tea (or Liubao tea) is a typical Chinese dark tea obtained via indigenous tea fermentation facilitated by microorganisms. Certain fungi present in Liupao tea that may produce CIT are a potential threat to consumer health. In the present study, various potential toxigenic mycoflora and the natural occurrence of CIT in Liupao tea were surveyed via the culture-dependent method, high performance liquid chromatography–fluorescence detection (HPLC–FLD), and ultrahigh performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS). Total mold counts ranged from 3.5 × 102 CFU/g to 2.1 × 106 CFU/g tea in 28 tea samples. A total of 218 fungal isolates belonging to five genera and 23 species were identified. Some of these strains, such as Aspergillus ochraceus, Aspergillus oryzae, Penicillium citrinum, and Penicillium chrysogenum, may potentially be a CIT-producing species. In addition, 32.7% of 113 Liupao tea samples were contaminated with CIT at concentrations ranging from 7.8 to 206.1 μg/kg. These CIT concentrations in Liupao tea are chiefly attributed to climatic conditions and water activity during storage that favor fungal proliferation and mycotoxin production. However, CIT could not be detected in Liupao tea stored for over 10 years. These results provide the first information about the potential toxigenic mycoflora and natural occurrence of CIT in Liupao tea. Therefore, storage conditions and fungal community must be monitored to ensure the quality of Liupao tea.
研究领域:
食品安全
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微生物学
·
植物学
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DOI
PubMed
高影响因子
Food Bioscience
·
2019
·
IF 3.22
Weilu Guo,Mouming Zhao,Qingjin Chen,Li Huang,Yan Mao,Ning Xia,Jianwen Teng,Baoyao Wei
The ability of a strain to produce citrinin was analysed in the growth inhibition zone using HPLC and UHPLC–MS. A total of 12 Penicillium citrinum strains isolated from Liupao tea were inoculated onto tea leaves and their capability for citrinin production were studied using different water content (15–45%) and temperatures (15–40 °C). The results provided a theoretical base for the safe manufacture of Liupao tea. Five strains of P. citrinum produced a clear growth inhibition zone with diameters of 11–22 mm. The citrinin contents in the liquid cultures of the 12 strains of P. citrinum ranged from 0.03 to 14.5 μg/mL. The citrinin contents of the teas with initial water content of 15 and 25% and those inoculated with P. citrinum were below the limit of detection during the fermentation. The citrinin content of the teas with initial water contents of 35 and 45% at 25 °C increased rapidly at first and then decreased during the fermentation. Similarly, the citrinin contents of the teas at 15 and 25 °C rapidly increased and then decreased. Meanwhile, P . citrinum was unable to produce citrinin at 35 and 40 °C. The optimum conditions were 45% water and 25 °C, generating the highest citrinin yield of 181 ng/g, which was higher than the limit of 50 ng/g citrinin in red yeast rice that can cause harm to human health. The highest citrinin yield in tea fermented for 50 days was 34.8 ng/g for stored teas with proper conditions. Therefore, storing teas with a low water content and temperature can reduce the content of citrinin.
研究领域:
食品科学
·
微生物学
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DOI
高影响因子
CARBON
·
2025
·
IF 11.6
Menghui Chi,Lingwen Gu,Ke Liu,Jing Lin,Qian Wang,Bing Yu,Ziyi Wang,Xueyun Fu,Daohao Li,Guiqiu Zhao,Cui Li
The photocatalytic antifungal strategy has attracted much attention in the field of fungicidal therapy due to its efficient therapeutic efficacy and avoidance of drug resistance. However, exploring safe and efficient photocatalysts remains an urgent challenge. In this work, nitrogen-doped carbon dots (N-CDs) combined with titanium dioxide (TiO 2 ) nanomaterials were synthesized by a hydrothermal method to exert a photocatalytic antifungal effect under sunlight irradiation. Compared with carbon dots/TiO 2 without N-doped modification, employing N doping to fabricate N-CDs/TiO 2 photocatalyst could further markedly optimize the bandgap structure of TiO 2 , improve the absorption of the solar spectrum and accelerate the electron-hole pairs separation, thereby greatly enhancing the photocatalytic reaction and antifungal effect. Remarkably, in the photocatalytic antifungal reaction, N-CDs/TiO 2 produces more oxygen radicals such as •OH and •O 2 − to rapidly hinder the germination of Aspergillus fumigatus hyphae and the formation of fungal biofilm. Additionally, the incorporation of N-CDs and TiO 2 possesses the great biocompatibility. Overall, this study proves that employing N-CDs/TiO 2 nanomaterials can be a promising and efficient therapeutic method against fungal infections.
研究领域:
微生物学
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材料科学
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医学
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产品关联
DOI
高影响因子
Scientific Reports
·
2025
·Vol. 15
·期 1
·1-11
·
IF 3.9
Zhang Liwen,Zhao Jinhong,Zhang Jie,Lou Bang,Li Huijie,Guo Fangyuan,Yang Gensheng,Hong Weiyong
The convergence of advanced materials science and additive manufacturing technologies has revolutionized medical applications ranging from orthodontic devices to patient-specific bone fixation systems. Despite these advancements, the clinical translation of 3D-printed orthopedic implants remains constrained by suboptimal biocompatibility of conventional manufacturing materials. This study systematically evaluates dental resin-based composites as alternative biomaterials for bone fixation devices, hypothesizing that their rapid photopolymerization kinetics, mechanical robustness, and inherent biocompatibility can address current limitations. Through stereolithography technology, ceftriaxone sodium-loaded bone fixation devices were developed with customized geometries tailored to diverse anatomical requirements. Material optimization revealed that a 7:3 ratio of ethoxylated bisphenol A dimethacrylate (BPA2EODMA) to triethylene glycol dimethacrylate (TEGDMA) achieved optimal mechanical performance. Cytocompatibility assessment using L929 fibroblasts demonstrated > 70% cell viability, confirming minimal cytotoxicity. The drug-eluting implants exhibited potent antimicrobial efficacy in 8-h elution assays, showing inhibition rates of 49.14 ± 4.89% ( Staphylococcus aureus ), 48.38 ± 5.88% ( Escherichia coli ), and 26.79 ± 2.69% ( Candida albicans ). These results validate the dual functionality of antibiotic-loaded dental resin constructs, positioning them as promising candidates for patient-specific orthopedic solutions that simultaneously address mechanical stability, osseointegration, and infection prevention.
研究领域:
生物医学工程
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材料科学
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药理学
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骨科
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产品关联
DOI
PubMed
高影响因子
CERAMICS INTERNATIONAL
·
2024
·
IF 5.1
Xiao Zong,Shuangshuang Wu,Kunji Lin,Jintao Zhang,Yehua Li,Donglin Lu,Xin Deng,Shengguo Lu,Jihua Qiu,Yuanzhi Shao,Shanghua Wu
Improving the thermal conductivities and bending strengths of ceramic substrates is crucial for their applicability as key components of integrated chips. Herein, we report the latest study in which the thermal conductivity and bending strength of aluminum nitride (AlN) ceramics were systematically predicted using high-precision prediction model approached with a machine learning (ML) method based on extreme gradient enhancement method (XGBoost). The ML model was used to rank the effects of the process parameters, and SHapley Additive exPlanations (SHAP) was employed to quantify the contributions of different factors. Sintering additives were found to have the predominant influence on the thermal conductivity and bending strength; particularly, those with cationic radii within 0.085–0.105 nm enhance both properties. By using Pr 2 O 3 as the sintering additive and adopting the preparation conditions recommended by the ML model, we prepared AlN ceramics with thermal conductivity as high as 195.63 W m −1 K −1 and bending strength of 371.60 ± 9.31 MPa, thus satisfying the application requirement of high thermal conductivity . The proposed model is also applicable to alumina and silicon nitride ceramics. This study provides a practicable and once-for-all strategy to realize the entire process from the ML prediction design to the preparation of ceramic materials exhibiting superior thermal conductivity and high strength.
研究领域:
材料科学
·
人工智能
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产品关联
DOI
高影响因子
ACS Applied Polymer Materials
·
2024
·
IF 4.4
Hongbo Zhao,Renjun Pei,Pei-Xi Wang
The teeth of limpets, consisting of orderly aligned hard goethite (iron oxyhydroxide) nanocrystals and flexible chitin microfibers, are one of the hardest and stiffest biomaterials found in nature. However, the replication of their composition and ordered microstructures to artificial biomimetic materials remains a challenge. Here, we fabricated large-sized limpet tooth analogues with hierarchically ordered structures by applying long-range orientational control to the short-scale self-assembly of hybrid colloidal liquid crystals formed by chitin nanocrystals and goethite nanorods. The obtained composite laminates exhibited excellent bending resistance compared to biomaterials, cement, and ceramics. These results suggest that the mass production of structurally ordered materials with high strength and toughness may be achieved by using lyotropic liquid crystals.
研究领域:
材料科学
·
生物学
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产品关联
DOI
高影响因子
Small
·
2024
·Vol. 20
·期 33
·2400482
·
IF 13
Wei Liu,Xi Yang,Guilong Yan,Li Wang,Jingjuan Lai,Zhenyu Li,Chunxia Zhao,Dong Xiang,Hui Li,Yuanpeng Wu
Humidity-responsive materials hold broad application prospects in sensing, energy production, and other fields. Particularly, humidity-sensitive, flexibility, and water resistance are pivotal factors in the development of optimized humidity-responsive materials. In this study, hydrophobic linear polyurethane and hydrophilic 4-vinylphenylboronic acid (4-VPBA) form a semi-intercross cross-linking network. This copolymer of polyurethane exhibits excellent humidity-sensitive, mechanical properties, and water resistance. Its maximum tensile strength and maximum elongation can reach 40.56 MPa and 543.47%, respectively. After being immersed in water at various temperatures for 15 days, it exhibited a swelling ratio of only 3.28% in water at 5 °C and 9.58% in water at 70 °C. While the presence of 4-VPBA network imparts humidity-sensitive, reversible, and multidirectional bending abilities, under the stimulus of water vapor, it can bend 43° within 1.4 s. The demonstrated material surpasses current bidirectional humidity actuators in actuating ability. Based on these characteristics, automatically opening waterproof umbrellas and windows, as well as bionic-arms, crawling robots, and self-propelled boats, are successfully developed.
研究领域:
材料科学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Nana Zhang,Anyan Wen,Xin Yang,Xinxin Zhang,Likang Qin,Haiying Zeng
Homogentisic acid (HGA), a product of the L-tyrosine metabolic pathway, serves as a substrate for tocopherol synthesis. Previous studies revealed that the fermentation of coix seeds by M . purpureus could simultaneously upregulate tocopherol and HGA levels. Therefore, this study focused on HGA to investigate its specific effect on tocopherol production during the fermentation process. The results showed that 0.02% Exo-HGA significantly increased tocopherol yield by 53.11%, reaching 184.808 μg/g. Based on the seed culture medium, the optimal formulation of key factors for promoting End-HGA synthesis in M. purpureus was determined. It was obtained through single-factor experiments and uniform design: D-fructose (36.816 g/L), L-tyrosine (0.656%), yeast extract powder (7 g/L), ZnSO 4 (0.654 g/L). Under these conditions, M. purpureus exhibited good growth, with an End-HGA yield reaching 844.461 μg/mL. In addition, fermentation of coix seeds with End-HGA led to an increase in the total tocopherol yield by 57.36%, reaching 181.303 μg/g. This result was comparable to that achieved with Exo-HGA addition. Therefore, enhancing End-HGA synthesis is an effective strategy to achieve a high tocopherol yield through M. purpureus fermentation. In conclusion, this study demonstrated that the regulation of microorganisms can enable high-level synthesis of target products and improve the efficiency of deep conversion and utilization of plant substrates.
研究领域:
生物化学
·
微生物学
·
食品科学
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产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2024
·
IF 4.9
Chenxi Ke,Yu Wu,Jiayao Ou,Hongda Zhu,Huiling Guo,Hongmei Sun,Mingxing Liu
Ochratoxin A (OTA) is a representative foodborne fungal toxin that poses great harm to humans, the ultra-sensitive detection of OTA is an urgent requirement for public health. In this work, a competitive colorimetric and ratio fluorescence dual-mode immunosensor for the detection of OTA based on 0D/2D AuAg NCs/SPCN/BGQDs heterojunction nanozyme was devised and fabricated. The presence of P-N heterojunctions was confirmed through electrochemistry and theoretical calculations, heterojunction formed between AuAg NCs-SPCN and BGQDs effectively augmented the oxidase-mimic activity, verified by enzyme kinetic analysis. Superoxide radicals (·O2-) were catalyzed by heterojunction nanozyme from dissolved oxygen, and o-phenylenediamine was oxidized by ·O2- to produce diaminophenazine. It exhibited an absorption peak at 450 nm overlapped with the emission peak of heterojunction nanozyme. The fluorescence at 434 nm was quenched through the IFE effect while the fluorescence at 565 nm was enhanced and accompanied by solution yellowing, so ratio fluorescence was formed due to two emission peaks and the dual-mode detection system was constructed. The dual-mode immunosensor exhibited exemplary linearity across the concentration span of 0.001–20 μg/L, with LODs of 0.28 ng/L (ratio fluorescence) and 0.2 ng/L (colorimetry) respectively. Therefore, this immunosensor demonstrated potential applications in food quality testing and visual analysis.
研究领域:
食品科学
·
生物化学
·
分析化学
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Liyang Zhang,Yangqin Gao,Ziyu Teng,Yida Liu,Xinming Liu,Chuanbo Cong,Kezhen Lai,Linping Li,Jipeng Dong,Ning Li,Lei Ge
Mimicking the movement of leaves in response to external stimulations is inspiring for designing photocatalyst system applicable in space- and weight-sensitive scenarios. Here, we demonstrate that by combining a novel gas–liquid film growth process with a tape assisted film transfer procedure driven by capillary force, a lightweight and twistable photocatalyst assembly can be easily constructed. Taking the gas–liquid interface as a solute transfer boundary to selectively trigger the film growth at the interface, BiOI and BiOI/In 2 O 3 thin films up to 15 cm in diameter can be grown at a solution surface. Thanks to the capillary force present at the dynamic contact front during the film pick-up procedure, the grown film can be peeled off from the solution surface with tape so that a lightweight BiOI/In 2 O 3 /tape assembly can be easily constructed. Due to its low density and the BiOI/In 2 O 3 heterojunction formed, the resulting BiOI/In 2 O 3 /tape assembly exhibits superior photocatalytic activity per gram towards NO removal. Furthermore, the capillary force driven film pick-up process also induces a tight contact between the film and the tape, which makes the assembly highly twistable: its structural integrity and photocatalytic activity are largely preserved after 10,000 twists. Utilizing the film’s flexibility and twistability, we assemble a temperature-, auto-folding/unfolding photocatalyst system by transferring the film onto a memory-alloy-based structure, which mimics the movement of leaves in response to external stimulations and makes the gas–liquid interface-derived BiOI/In 2 O 3 /tape assembly suitable for use in space- and weight-limited environments.
研究领域:
材料科学
·
环境科学
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产品关联
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Yang Zhang,Caihao Wang,Ning Cao,Yang Liu,Chaocheng Zhao,Yongqiang Wang,Shaobin Wang,Jinqiang Zhang
Symmetrical planar unit cells and periodic layered structures of polymeric photocatalysts are the roots of poor light absorption and severe carrier recombination in photochemical process, which in turn diminish redox capabilities during photocatalytic overall water splitting. In this study, we synthesized porous and curled carbon nitride nanosheets (U-A400) featuring imperfect molecular structures through polycondensation of an asymmetric precursor molecule and a soft template. The twisted framework in U-A400 promoted strong n → π* electron transitions, generating a considerable built-in electrical field and prolonging the average fluorescence lifetime. Additionally, the structural distortion increased the number of surface acid-base active sites. Consequently, these adjustments in the micro- and topological structures rendered U-A400 highly effective for photocatalytic overall water splitting, achieving H 2 and O 2 average evolution rates of 65.93 and 38.53μmol g −1 h −1 , respectively, over 4 h of visible light (λ > 420 nm) irradiation, 38 times higher than those of graphitized carbon nitride. This work presents valuable insights into the development of advanced polymeric photocatalysts, efficiently harnessing sunlight for overall water splitting, thereby contributing to sustainable energy conversion and clean fuel production.
研究领域:
材料科学
·
能源化学
✓ 已验证
产品关联
DOI
高影响因子
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