PROCESS BIOCHEMISTRY
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2022
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IF 4.885
Zhengyu Hu,Ruiying Yu,Jinfeng Sun,Yuanqi Duan,Hongli Zhou,Wei Zhou,Gao Li
In this study, polysaccharides from the leaves of Rhododendron dauricum L. (RDPs) were decolorized using the static macroporous resin adsorption method. The RDPs were characterized and their antioxidant activities were investigated. An analytical hierarchy process (AHP) and a response surface method (RSM) were used to determine the optimum decolorization conditions as: a polysaccharide concentration of 8 mg/mL, decolorization time of 70 min, liquid–solid ratio of 6 mL/g, and decolorization temperature of 52 °C, with D101 as the best resin. Furthermore, two novel polysaccharides (RDP-1 and RDP-2) were isolated from R. dauricum leaves and characterized, which demonstrated that RDP-1 and RDP-2 were both composed of mannose , glucose, galactose , and arabinose in different molar ratios. These RDPs also exhibited a triple helix conformation, and primarily contained →6)-Glc p -(1→, →5)-Ara f -(1→, →2,4)-Man p -(1→, →6)-Gal p -(1→ and Glc p -(1→ residues with different connection sequences. Biological activity tests showed that the two polysaccharides possessed scavenging capacity against 1,1-Diphenyl-2-picrylhydrazyl (DPPH), hydroxyl, and 2,2-Azino-bis-(3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) radicals. The results indicated that the antioxidant activities of the RDPs obtained by the static decolorization method improved, which lays a theoretical foundation for developing techniques for utilising R. dauricum .
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SEPARATION AND PURIFICATION TECHNOLOGY
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2022
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IF 9.136
Kaiming Fan,Yanling Liu,Xiaoping Wang,Peng Cheng,Shengji Xia
In this study, three different kinds of nanofiltration membranes with polyamide (PA), polyesteramide (PEA), and polyester (PE) active layers were respectively synthesized by using piperazine, serinol and meso -erythritol as the aqueous monomers for interfacial polymerization. The three types of membranes achieved comparable rejections for the reference organic solute (i.e., xylose) via optimization of preparation conditions, based on which the characteristics of different active layers were comprehensively investigated. Results showed that the PA, PEA and PE membranes exhibited distinct surface morphologies. The special surface structures of PEA membranes and the larger active layer thickness of PE membranes resulted in their lower water permeance compared to PA membranes. Different from PA active layers, the absence of positively charged regions in the PEA and PA active layers was beneficial for the passage of divalent cations and the rejection of NaCl. Moreover, despite the comparable mean pore size, PA membranes exhibited a more uniform membrane pore size resulting in a higher rejection of organic matters in natural water than PEA and PE membranes. This study presented the differences in physicochemical properties and separation performance among the different membrane active layers, which could provide references for the rational selection of nanofiltration membrane types during application.
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高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
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2022
·Vol. 58
·期 4
·2115-2129
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IF 3.612
Jing Dai,Zhuqian Xiao,Jiajie Li,Qing Ge,Hongpeng Wang,Ruyi Sha,Jianwei Mao
Summary In this study, crude polysaccharide (BSSP) was extracted from bamboo ( Phyllostachys edulis ) shoot shell using the ultrasonic-assisted phosphotungstic acid hydrolysis method. Then, BSSP was further fractionated by DEAE Sepharose fast flow column and serial NaCl solution elutions to obtain two fractions, BSSP-A and BSSP-B. The results showed that the maximum yield of BSSP was 195.2 mg g −1 (19.52%) under optimised conditions. Structural analysis indicated that both BSSP-A and BSSP-B were heteropolysaccharides with α-pyranoside bond structure and mainly consisted of xylose by scanning electronic microscopy, Fourier transform infrared spectroscopy, ultraviolet–visible spectroscopy, thermal characterisation and nuclear magnetic resonance spectroscopy. BSSP-B showed a better protective effect than BSSP-A against H 2 O 2 -induced oxidative injury in WRL-68 cells by protecting the cell membrane structure, maintaining mitochondrial membrane potential and increasing intracellular antioxidant enzymes activities. Immunoblotting indicated that polysaccharides might up-regulate the expression of antioxidant enzymes through the Keap1/Nrf2 signalling pathway and thus alleviate H 2 O 2 -induced cell injury. The findings of this study suggested that polysaccharides from bamboo shoot shell may act as potent antioxidants in functional food industries.
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CELLULOSE
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2022
·Vol. 29
·期 15
·8133-8150
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IF 6.123
Sun Liangyun,Han Juan,Tang Chaofan,Wu Jiacong,Fang Sihan,Li Yuanyuan,Mao Yanli,Wang Lei,Wang Yun
As an effective substitute for ionic liquids (ILs), deep eutectic solvents (DESs) are known as a new generation of green solvents. In addition, DESs are considered to be the best solvents to replace traditional organic solvents and ILs due to their advantages of economy, simple preparation and biodegradability, which can be used to further break through the technical bottleneck in the field of biomass pretreatment. This study synthesized a series of choline chloride-based (ChCl-based) DESs and used them to pretreat microcrystalline cellulose (MCC) without dissolution, in order to achieve the purpose of improving the saccharification effect. DES-9 (ChCl/hydroquinone), DES-12 (ChCl/acetamide) and DES-16 (ChCl/acetic acid) were better pretreatment solvents. The optimum conditions for the pretreatment of MCC by DES-9 and DES-12 were both at 110 °C for 4 h, and the optimum condition of DES-16 was at 90 °C for 4 h. Compared with untreated MCC (26.66%), the saccharification yield of pretreated MCC with DES-16 increased by 20.5%. Finally, the structure and morphology changes of MCC before and after pretreatment were investigated in detail through the degree of polymerization (DP), SEM and enzyme adsorption. The results showed that DESs pretreatment made the surface of MCC become rough, which enhanced the accessibility of cellulose and enzymes, improving the saccharification effect. Graphical abstract
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ACS Sustainable Chemistry & Engineering
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2022
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IF 9.224
Jiansu Ran,Ruixue YangCheng,Yuntong Cui,Jianjian Wang
Selective and efficient low-temperature hydrodeoxygenation (HDO) of lignin derivatives in water for the production of valuable biofuel and chemicals is still challenging. Herein, we reported biomass carbon-supported ultrafine Pd nanoparticles as highly active and stable catalysts for low-temperature HDO of vanillin (a typical lignin-derived compound) to 2-methoxy-4-methylphenol (MMP). We found that the abundant carboxyl groups on the resultant catalyst greatly accelerated the conversion of the generated intermediate (vanillyl alcohol, VAL), and then, the synergistic catalysis between carboxyl groups and Pd nanoparticles promoted the efficient production MMP through a free-radical pathway. Other lignin derivatives with different functional groups were also efficiently converted to the corresponding products over the prepared catalyst. This work may provide a new idea for designing a biomass-derived catalyst for efficient transformation of various lignin derivatives to produce value-added biofuels and chemicals.
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ACS Applied Materials & Interfaces
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2022
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IF 10.383
Hao Chao,Zixin Zhou,Wenting He,Meng Li,Xiaoyu Yuan,Ping Su,Jiayi Song,Yi Yang
Although capsule-like materials as host carriers for enzyme encapsulation have been a hot topic in recent years, creating an ideal microenvironment for enhanced enzymatic performance is still a formidable challenge. Herein, we created a template-free method to in situ encapsulate natural enzymes in hollow covalent organic framework (COF) capsules at room temperature. The COF crystallites migrated from the inner core and self-assembled at the outside walls during the inside-out Ostwald ripening process, retaining the enzymes in the cavity. The adjustable hollow structure of the enzyme@COF capsule allowed the basic vibration of the enzyme to maintain a certain degree of freedom, thus significantly enhancing the enzymatic bioactivity. The hollow enzyme@COF capsule has large mesoporous tunnels allowing the efficient transport. In addition, the enzyme encapsulated in the capsule showed superior activity and ultrahigh stability under various extreme conditions that may lead to enzyme inactivation, such as high temperature, organic solvents, chelates, and the denaturing agent. Finally, the prepared hollow GOx@COF capsule was used for electrochemical sensing of glucose in human serum, and the electrochemical sensor exhibited high selectivity and satisfactory test results. This research not only provides a new way for COFs to encapsulate enzymes but also has potential applications in biocatalysis and biosensing, making artificial organelles possible.
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2022
·
IF 8.025
Meng-Ting Kuang,Jing-Yue Xu,Jin-Yu Li,Liu Yang,Bo Hou,Qi Zhao,Jiang-Miao Hu
A novel polysaccharide (MSP-1) was isolated from the fruiting body of Morchella sextelata and purified using DEAE-52 and Sephadex G-75. The molecular weight of MSP-1 was 1.17 × 10 4 Da, as detected by HPLC analysis. The monosaccharide composition of MSP-1 was mannose and glucose at a ratio of 1.00: 1.25. Methylation and NMR results revealed that the backbone of MSP-1 was composed of →4)- β -D-Man p -(1→, →4)- β -D-Glc p -(1→, →4)- α -D-Glc p -(1→, and →4, 6)- α -D-Glc p -(1→. SEM images of MSP-1 presented a dense network structure with porous characterizations. The immunomodulatory activities of MSP-1 were evaluated using RAW264.7 cells, and the results showed that MSP-1 promoted proliferative and phagocytic activity and increased the production of NO, TNF- α and IL-6. These results indicated that MSP-1 exhibited significant immunomodulatory activities.
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PubMed
高影响因子
RENEWABLE ENERGY
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2022
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IF 8.634
Yanting Lv,Zhengyu Chen,Huan Wang,Yongcang Xiao,Rongxin Ling,Murong Gong,Weiqi Wei
In this work, an HCl-catalyzed ethylene glycol (EG) pretreatment was exploited for promoting sugar release from sugarcane bagasse (SCB). The results showed that the combination of HCl and EG could remove the xylan (∼100.0%) and lignin (∼61.3%) in SCB together, which finally resulted in the pretreated substrate having a good efficacy for follow-up enzymatic hydrolysis . The maximum glucose yield of this work was 93.9% and obtained after pretreatment at 130 °C for 60 min with 0.5% HCl and 72 h enzymatic digestion. The analysis of various pretreated or un-pretreated SCB indicated that the changes of surface morphology and internal compositions of SCB were the mainly reasons for its hydrolysis efficiency enhancement. The addition of Tween 80 into hydrolysis process could remarkably shorten hydrolysis time and cellulase dosage from 72 h to 36 h and 20 FPU/g substrate to 10 FPU/g substrate, respectively, meanwhile maintaining a relatively high glucose yield (91.4%).
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RENEWABLE ENERGY
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2022
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IF 8.634
Lincai Peng,Xin Huangfu,Yao Liu,Huai Liu,Junhua Zhang
The processing of C6 sugars to alkyl levulinate (AL), a versatile biomass-derived building block, can be readily implemented via the straightforward acid-catalyzed reaction in alcohol medium while it remains a challenge to produce AL from C5 sugars in the one-pot procedure over a monolithic catalyst. In this contribution, a budget and multifunctional natural lignocellulose welded Zr–Al bimetallic hybrid (ZrAl@CS-MSA) has been successfully constructed with crude corn stalk as a sustainable support. The becoming Lewis and Brønsted acid sites of ZrAl@CS-MSA catalyst guarantee a high AL yield of 54.3% from xylose via a one-pot process in a renewable n -butanol medium. Experimental findings revealed that the sulfonic group of ZrAl@CS-MSA plays a significant role in the xylose dehydration, furfuryl alcohol etherification and alcoholysis reactions while Zr species is in charge of the transfer hydrogenation of furfural. The Al species enclosed in ZrAl@CS-MSA can regulate its Brønsted and Lewis acidity, accelerating the etherification and alcoholysis reactions as well as alleviating the over hydrogenation of AL. This work opens a green, sustainable and reliable avenue for producing C5 sugar-based AL using natural lignocellulose-related multifunctional catalysts.
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APPLIED CATALYSIS B-ENVIRONMENTAL
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2022
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IF 24.319
Di Li,Zengyong Li,Ren Zou,Ge Shi,Yiming Huang,Wu Yang,Wang Yang,Chuanfu Liu,Xinwen Peng
Developing efficient bifunctional electrocatalyst for overall water splitting and conversion of biomass into value-added products at large current density is essential but challenging. Herein, a hierarchical Fe-doped Ni 2 P nanosheets hybridized with C on Ni foam (Fe-Ni 2 P@C/NF) was developed as a robust bifunctional catalyst with excellent catalytic activity for hydrogen evolution reaction (HER) with overpotentials of 75 and 313 mV at 10 and 1000 mA cm −2 , respectively, and as well as for OER with a lower overpotential of 269 mV at 400 mA cm −2 . Particularly, the integrated double-electrode electrolyzer for simultaneous HER and biomass-derived monosaccharide oxidation reaction (MOR) using Fe-Ni 2 P@C/NF only requires a low voltage of 1.55 V to drive a current density of 100 mA cm −2 . Meanwhile, the value-added lactic acid is obtained at the anode. This strategy of coupling biomass oxidation to promote H 2 production can significantly reduce energy consumption and obtain additional high value-added products.
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高影响因子
CELLULOSE
·
2022
·Vol. 29
·期 2
·879-891
·
IF 6.123
Zhou Liang,Liu Qiyu,Ma Qiaozhi,Guan Mingzhao,Ouyang Xinping,Qiu Xueqing
Abstract Saccharification of cellulose into glucose is a key step for the utilization of lignocellulose. Molten salt hydrate (MSH) is unique in selective hydrolysis of cellulose into glucose but the separation of formed glucose from MSH is challenging due to the high solubility of glucose in MSH. To address this issue, this work developed a method where a small dosage of MSH was applied to impregnate cellulose and convert it into water-soluble sugar with the degree of polymerization (DP) lower than 6, which can be readily hydrolyzed into glucose under mild conditions without sacrificing selectivity. Under the optimized impregnation condition at 110 °C for 2 h, cellulose was selectively converted into water-soluble sugar with a yield of 87.5%. Then the MSH can be efficiently dissolved and removed by anti-solvent isolation with n -butanol, resulting in a sugar recovery of 84.3%. We showed that the separation efficiency was influenced by anti-solvent polarity and oligosaccharides chain-length. Moreover, the production and separation of water-soluble sugar from corn stover was also investigated. 88.9% yield of water-soluble sugar was obtained from the impregnation of corn stover under the optimized reaction conditions. With the addition of n -butanol as an anti-solvent, the water-soluble sugar can be recovered from MSH with a selectivity of 86.7%. Graphical Abstract
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ACS Sustainable Chemistry & Engineering
·
2022
·
IF 9.224
Hui Yang,Chaonan Tao,Lincai Peng,Junhua Zhang,Liang He,Huai Liu
It remains a challenging task for the efficient conversion of carbohydrates to value-added chemicals at concentrated feedstock solution. In this contribution, the acid-catalyzed processing of high-concentration C5/C6 sugars (180 g/L) in the ethylene glycol (EG) solvent system was investigated, and prominent discrepancy was noticed between C5 and C6 sugars. A large amount of liquefied products was achieved in the concentrated C6 sugars EG solution, whereas the main products in the case of C5 sugars were found to be solid humins. The reaction mechanism studies revealed that EG can shelter fructose and reaction intermediates (5-hydroxymethylfurfural and levulinic acid) from the cross-polymerization and self-etherification reactions, thereby reducing the generation of solid humins during the fructose liquidation process. However, in the case of xylose, the copolymerization between EG and furfural (FF) generates a solid resin, thus rendering a high solid product yield. Moreover, the produced FF–EG resin was found to be a polymer with high molecular weight formed by connecting the furan ring and the alkyl structure through an ether bond. This finding provides insights into the acid-catalyzed conversion of concentrated C5/C6 sugars in EG, facilitating rationally designed processes for the valorization of concentrated carbohydrates.
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