JOURNAL OF FOOD PROCESS ENGINEERING
·
2020
·Vol. 43
·期 8
·e13449
·
IF 1.703
Abdul Rahaman,Xin-An Zeng,Muhammad Adil Farooq,Ankita Kumari,Mian Anjum Murtaza,Nabeel Ahmad,Muhammad Faisal Manzoor,Sadia Hassan,Zahoor Ahmad,Chen Bo-Ru,Zhan Jinjing,Azhari Siddeeg
The aim of this study was to investigate the effect of pulsed electric fields (PEF) on bioactive compounds and physiochemical properties present in apricot juice. In which 0, 7, and 14 kV/cm of PEF intensity were applied to treat the juices with a flow rate of 40 ml/min, frequency of 1 kHz at 25 ± 2°C for 500 μs. Thereafter the phenolics, flavonoids, antioxidant volatile compounds, and physiochemical properties of juice were analyzed. Furthermore, the change in functional groups of biological compounds was investigated by Fourier-transform infrared spectroscopy. Results indicated no significant change in pH, °Brix, and color in PEF treated sample even at increased PEF intensities as compared to untreated. A significant increase in cloud value, phenolic compounds, flavonoids, and antioxidant activity of the PEF treated sample was observed. A decrease in the browning index was observed as compared to unprocessed samples. The little variation in biological compounds was observed in PEF treated sample. Practical Applications Nowadays, researchers are paying more attention to non-thermal techniques for the processing of food products, which could enhance the nutritional profile and shelf life stability of food products. PEF retains the heat-sensitive bioactive constituents, extends the shelf life in order to achieve the fresh like quality of liquid products. This research proved that PEF treatment significantly increased the cloud value, phenolic compounds, flavonoids, antioxidant activity, and helps to retain the bioactive compounds present in apricot juice. Furthermore, this present research would also provide valuable data that can be helpful for food industries to design and select PEF technology to process the liquid-based food product like fruit juice.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 37
·31101-31106
·
IF 3.289
Ailing Hui,Shijing Zhu,Huayang Yin,Li Yang,Zheng Zhang,An Zhou,Jian Pan,Wencheng Zhang
A series of ginkgolide B derivatives (GBD) were designed for delivery of ginkgolide B (GB) across the blood–brain barrier to the brain. Three GBD had shown considerable brain-targeting ability in our previous study. Among them, the ginkgolide B pyrazine (PGB) derivative was selected as a promising novel GB derivative because of its highest brain distribution. Further investigations with regard to PGB and GB's effects on inhibiting the expression of P-gp and attenuating the efflux function of P-gp were reported in this study. They were considered a big step towards better understanding the relationship between GBD's brain-targeting behavior and P-gp regulation. Western blot analysis indicated that PGB was more effective in inhibiting the expression of P-gp than GB in rat brain microvessel endothelial cells (rBMECs) and rat cerebral cortexes and hippocampus. Moreover, the efflux function of P-gp via the transportation of rhodamine 123 was significantly inhibited in PGB-treated rat brains. Preventive medication with PGB induced a better protection of the integrity of the BBB in incomplete ischemia mice. Our findings revealed that an attenuated expression level and efflux function of P-gp and improved protective effect could be realized by the ginkgolide B derivative. These investigations will pave the way for designing more efficient brain-targeting derivatives of ginkgolide and some other central nervous system drugs.
研究领域:
药理学
·
神经科学
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING & TECHNOLOGY
·
2016
·Vol. 39
·期 7
·1301-1308
·
IF 2.385
Yuan Gang Zu,Lingling Wang,Xiuhua Zhao,Yuanyuan Li,Weiwei Wu,Chang Zu,Yannian Huang,Mingfang Wu,Ziqi Feng
The content of flavonoids in Gingko biloba extract (GBE) could be increased by antisolvent recrystallization (ASR) using ethanol as solvent and hexane as antisolvent. By means of the single-factor method, the effect of main factors on the content of flavonoids in purified GBE was investigated. Under optimal conditions, the content of flavonoids and ginkgo terpene lactones in purified GBE could be significantly enhanced. In addition, the content of total ginkgolic acid in purified GBE was reduced. The morphologies of raw and purified GBE were characterized by scanning electron microscopy. It was found that the particle size of purified GBE was much smaller than that of raw GBE. At the same time, the saturated solubility could be improved by more than four times.
研究领域:
植物化学
·
药物化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FOOD PROCESS ENGINEERING
·
2019
·Vol. 42
·期 8
·e13299
·
IF 1.448
Muhammad Faisal Manzoor,Nazir Ahmad,Rana Muhammad Aadil,Abdul Rahaman,Zahoor Ahmed,Abdur Rehman,Azhari Siddeeg,Xin-An Zeng,Ahsan Manzoor
The effects of the pulsed electric field (PEF) on the rheological, structural, and physicochemical properties of almond milk were evaluated. Results indicated that there was a significant increase in electrical conductivity, cloud value, and cloud stability, while a significant decrease was observed in viscosity and nonenzymatic browning after PEF treatments. The particle size was significantly reduced and monodisperse distributions were observed, particle size reduction further confirmed by Scanning Electron Microscopy. Whiteness Index, clarity, and physical stability of samples were improved, without affecting either protein stability or viscosity. The rheological behavior of all samples was described using a Herschel–Bulky model. PEF treatments increased the apparent viscosity and changed the consistency index ( K ). The denaturation temperatures and apparent enthalpy of almond milk samples were modified by PEF treatments. This study indicated that dispersive and aggregative properties of PEF treated almond milk were different due to differences in particle coagulation, protein denaturation and morphological characteristic of aggregates. Practical applications Now a day, green processing techniques, such as PEF is used for processing the different types of beverages. The outcomes of the recent study described that PEF treatment could improve the physicochemical properties, reduce the particle, physical stability, and whiteness index of almond milk. The results of this research proposed that PEF has an ability to enhance the quality of almond beverage and may be employed for the processing at an industrial scale.
研究领域:
食品科学
·
加工技术
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Xiulian Li,Xinguang Fan,Yingjie Zhuo,Jiteng Ma,Kuanbo Cui,Chanchan Sun,Fengjun Guo
The carotenoid biosynthesis and phenolic metabolism were studied to explain the effect of methyl salicylate (MeSA) on the lipophilic antioxidant capacity (LAC) and hydrophilic antioxidant capacity (HAC) in apricot during postharvest storage. Our results indicated that the HAC of apricot was higher than LAC and mainly responsible for total antioxidant capacity of apricot. Preharvest spraying of MeSA (0.2 mmol L −1 ) could improve the value of HAC but declined LAC of apricot. The enhanced HAC in MeSA treated apricot was positively related to the increased content of phenolics, especially to (+)-catechin, which was catalyzed by the enzymes related to phenolic metabolism. While, the decline of LAC in apricot treated by MeSA could be attributed to the inhibition of carotenoids accumulation, which was regulated by carotenogenic genes. We concluded that MeSA could affect the lipophilic and hydrophilic antioxidant capacity of apricot by regulating carotenoid biosynthesis and phenolic metabolism.
研究领域:
植物学
·
食品科学
·
园艺学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2023
·Vol. 12
·期 21
·4005
·
IF 5.2
He Wang,Yue Zheng,Xinyue Tang,Ting Zhang
Loquat is a nutrient-rich fruit with juicy and sweet pulp, but it is vulnerable to rot and deterioration without proper postharvest preservation measures. This study aimed to improve the postharvest quality of loquat by developing a microemulsion system based on an essential oil extracted from theTorreya grandiscv. Merrillii aril (TaEO), which has antimicrobial and antioxidant properties. An optimal TaEO microemulsion (TaEO-ME) was formulated, using a mixture of Tween-40 and Tween-80 as the surfactant, 1-butanol as the co-surfactant, and TaEO as the oil phase, with mass ratios of 9:1, 3:1, and 6:1, respectively. Two TaEO-ME formulations with 60% and 70% water contents were stable for 180 days at room temperature, with a mean droplet size below 12 nm and polydispersity index less than 0.24. They also exhibited higher stability and enhanced biological activities compared to free TaEO. Loquat fruit treated with TaEO-ME displayed a reduced decay index and lower membrane lipid peroxidation after 15 days of storage at 15 °C, as indicated by the lower malondialdehyde content and higher peroxidase activity. Moreover, the TaEO-ME treatment preserved the nutrient quality by maintaining the total phenolic compounds and ascorbic acid content. Our findings suggested that TaEO-ME can be used as a substitute for chemical preservatives to keep fruits fresh.Keywords:microemulsion;Torreya grandiscv. Merrillii aril;essential oil;loquat;fruit quality
研究领域:
食品科学
·
植物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING RESEARCH & DESIGN
·
2023
·
IF 3.9
Rui Hao,Haixia Ji,Li Gao,Jiahui Chen,Ying Shi,Junyan Yang,Changchun Yuan
Pollution of water resources has become a global environmental problem, and the massive discharge of high-color printing and dyeing wastewater has caused serious harm to the natural environment. In this work, we have used repeated alkali solubilization and acid precipitation for an agricultural and forestry by-product, bilberry kernel shells, to obtain Mountain apricot shell Melanin (Mas-Mel), which can be used for the adsorption of cationic dyes. By graft copolymerization with a natural sugar-based polymer, κ-carrageenan (κCA), in a mild manner, we have prepared the adsorbent, Mountain apricot shell Melanin /κ-carrageenan (Mas-Mel/κCA) hydrogel beads, which were physically and chemically analyzed using a variety of spectroscopic and microscopic techniques. The optimum conditions for methylene blue (MB) adsorption and malachite green (MG) on Mas-Mel/κCA hydrogel were: contact time 150 min, adsorbent dose 60 mg, pH 6–7. The results of the adsorption tests followed the Pseudo-second order kinetics (R 2 = 0.97 and 0.95), and the maximum adsorption capacity of MB and MG based on the Langmuir isotherm model was 48.63 mg/g and 37.84 mg/g, respectively. In addition, the prepared hydrogel beads still exhibited a high reusability and removal rate (∼50 %) after three consecutive 400-minute cyclic adsorption-resolution cycles. Our results may provide a new strategy for the development of green bio-based adsorbent materials and industrial dye wastewater treatment .
研究领域:
环境科学
·
材料科学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
PHYTOCHEMICAL ANALYSIS
·
2024
·Vol. 35
·期 6
·1428-1442
·
IF 3
Sijie Zhang,Xingchu Gong,Haibin Qu
Introduction Previously reported preparation methods of Ginkgo biloba leaf extract (EGBL) have mainly focused on the enrichment of flavonoid glycosides (FG) and terpene trilactones (TT), which led to the underutilization of G. biloba leaves (GBL). Objectives To make full use of GBL, in this study, a comprehensive optimization strategy for preparing EGBL by macroporous resin column chromatography was proposed and applied to enrich FG, TT, and shikimic acid (SA) from GBL. Methodology Initially, the static adsorption and desorption were executed to select suitable resin. Then, the influences of solution pH were investigated by the static and dynamic adsorption. Subsequently, eight process parameters were systematically investigated via a definitive screening design (DSD). After verification experiments, scale-up enrichment was carried out, investigating the feasibility of the developed strategy for application on an industrial scale. Results It was found that XDA1 was the most appropriate adsorbent for the preparation of EGBL at solution pH 2.0. Furthermore, based on the constraints of the desired quality attributes, the optimized ranges of operating parameters were successfully acquired, and the verification experiments demonstrated the accuracy and reliability of using DSD to investigate the chromatography process for the preparation of EGBL. Finally, magnified experiments were successfully performed, obtaining the EGBL containing 26.54% FG, 8.96% TT, and 10.70% SA, which reached the SA level of EGB761, an international standard EGBL. Conclusion The present study not only provided an efficient and convenient approach for the preparation of EGBL enriched in SA but also accelerated efforts to high-value utilization of GBL.
研究领域:
植物化学
·
中药学
·
分离纯化技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BEHAVIOURAL BRAIN RESEARCH
·
2025
·
IF 2.3
Yue-Heng Yan,Hong-Kun Wang,Zi-Hao Wang,Rui-Ze Wang,Ruo-Xuan Li,Li-Li Huang,Yan-Yan Wang
NMDA receptors (NMDAR) are vital in CNS activities such as anxiety, memory, and cognition, and the neurobiological mechanisms behind anxiety disorders are exceedingly complicated. The "glutamic acid theory" posits that glutamate excitotoxicity is a key pathophysiological mechanism in anxiety disorders. However, the exact mechanism by which conditioned fear contributes to anxiety disorders remains unknown.Based on the conditioned fear-induced anxiety disorder model, this work aims to investigate changes in NMDAR and related proteins throughout the acquisition and expression of fear memory, as well as the impact on synaptic structural and functional plasticity. Injecting the NMDA receptor endogenous agonist D-Serine (50 μg/μL) and the noncompetitive antagonist MK-801 (1 μg/μL) into the lateral ventricle of the conditioned fear model rats, as well as conducting behavioral observations, show that NMDAR are closely involved in the development of conditioned fear-induced anxiety. Model rats showed significant changes in glutamate (Glu) and γ-aminobutyric acid (GABA) levels in the amygdala (BLA), as well as expression of NMDAR and downstream BDNF/TrkB signaling pathway components. At the same time, model rats exhibited synaptic and neuronal injury, aberrant long-term potentiation (LTP), and decreased expression of essential synaptic proteins SYP and PSD-95. In conclusion, our study demonstrates that NMDAR and synaptic plasticity play a critical role in the development of conditioned fear-induced anxiety, serving as an important reference for understanding the neurobiological underpinnings of anxiety disorders and providing insights into their treatment and new possible targets.
研究领域:
神经科学
·
行为学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2020
·Vol. 101
·期 8
·3290-3297
·
IF 2.614
Isaac Duah Boateng,Xiao-Ming Yang
BACKGROUND Ginkgo biloba seeds are used as a functional food across Asia. However, the presence of toxic compounds has limited their application. In this study, freeze drying, infrared drying, hot-air drying and pulsed-vacuum drying were used to dry G. biloba seeds. A comprehensive analysis was performed on their product quality, antioxidant activities, bioactive and toxic components. RESULTS Results showed that the drying methods had a significant influence on product quality with freeze drying being superior due to the minimal microstructural damage, followed by infrared drying and pulsed-vacuum drying. Infrared-dried product possessed the strongest antioxidant activities and higher bioactive compound content than hot-air-dried and pulsed-vacuum-dried product. Toxic compounds in fresh G. biloba seeds (ginkgotoxin, ginkgolic acid and cyanide) were reduced markedly by drying. Ginkgotoxin was reduced fourfold, and the contents of acrylamide, ginkgolic acid and cyanide in dried G. biloba seeds were reduced to the scope of safety. Amongst the four drying methods, infrared drying had the shortest drying time, and its product showed higher quality and bioactive compound content, and stronger antioxidant activities. CONCLUSIONS These findings will offer salient information for selecting a drying method during the processing of ginkgo seeds. Infrared drying could be considered as a multiple-effect drying method in the processing of ginkgo seeds. © 2020 Society of Chemical Industry
研究领域:
食品科学
·
植物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2019
·Vol. 43
·期 2
·514-523
·
IF 2.516
Honghong Li,Wencheng Zhang,Zeyu Wu,Xusheng Huang,Ailing Hui,Yiwen He,Haiyan Wang
Ginkgolide B is in great demand worldwide on account of its extensive and excellent pharmacological effects, however, it is difficult to separate and purify ginkgolide B. In this study, ginkgolide B molecularly imprinted polymers were prepared by combining software simulation and molecular imprinting technique, and its characterization and adsorption performed evaluation were performed to understand the adsorption behavior of the polymers. The adsorption equilibrium concentration of molecularly imprinted polymers was 0.70 mg/mL, and the adsorption equilibrium time was 4 h. Meanwhile, the adsorption isotherm of the polymers for ginkgolide B fitted well with the Langmuir model, and the adsorption kinetics was in line with the pseudo-second-order kinetics. In contrast, the adsorption capacity of molecularly imprinted polymers on ginkgolide B was higher than that of non-molecular imprinted polymers, with better selectivity and better adsorption after repeated use for six times. The application experiments showed that molecular imprinted polymers have a good adsorption effect in low purity samples. Therefore, the polymers reported herein can be expected to apply in the adsorption and separation of ginkgolide B samples.
研究领域:
药学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2018
·Vol. 8
·期 39
·22136-22145
·
IF 2.936
Jiawei Han,Xin Wang,Jingxian Wang,Lingchong Wang,Lihua Chen,Junsong Li,Wen Li
The Quality-by-Design (QbD) approach was employed to investigate the fluid-bed coating process for the conversion of ginkgo lactone (GL) liquid nanosuspensions into dried nanosuspensions. The effects of critical process variables including inlet air temperature, inlet air capacity and atomizing air pressure were investigated. The particle size and percent yield were optimized using a full factorial design. A Box-Behnken design (BBD) was employed to generate the response surface and optimize process conditions. Multi-linear regression and one-way ANOVA were used to analyze the relationship between critical variables and responses. The results showed that all three selected variables were significant factors (p < 0.05) affecting the particle size. Higher inlet temperature, inlet air capacity or atomizing air pressure will cause an increase of particle size. In addition, the percent yield primarily depended on the inlet air temperature and inlet air capacity (p < 0.05). A higher percent yield was obtained at a higher inlet air temperature or inlet air capacity. The optimal conditions for BBD, including inlet air temperature, inlet air capacity and atomizing air pressure, were set at 40 °C, 11.6 Nm3 and 0.7 bar, respectively. Compared with the raw GLs, the optimized products presented an amorphous state and possessed much faster dissolution. The particle size, percent yield, PDI, zeta-potential and redispersibility index of the optimized products were 254.3 ± 9.8 nm, 82.36 ± 1.87%, 0.155 ± 0.02, −32.9 ± 3.8 mV and 113 ± 4.4% (n = 3), respectively. These results indicate that fluid-bed coating technology based on a QbD approach was sufficient for the solidification of nanosuspensions.
研究领域:
制药工程
·
药物化学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）