JOURNAL OF MATERIALS RESEARCH
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2023
·Vol. 38
·期 11
·2863-2873
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IF 2.7
Zhang XiaoNan,Yang Zhongxin,Xu Wenqin,Li Xing,Pan Guanghua,Chen Nan-Chun,Xie Qing-Lin,Wang Xiu Li
Potassium diformate (KDF) has broad prospects in replacing common antibiotics in animal feed; however, the utilization rate of KDF in the animal intestine is low. Therefore, it is necessary to construct a drug delivery system for its targeted release in the colon. When Zeolite P was used as a drug-binding effector, KDF was uniformly dispersed in the pectin matrix, and the pectin bacteriostatic microspheres loaded with KDF were prepared using the emulsion cross-linking method. The optimized pectin bacteriostatic microspheres were systematically characterized, we observed that the drug loading rate and stability of pectin bacteriostatic microspheres improved significantly after adding Zeolite P and the utilization rate of KDF in the simulated intestine was higher than that in the stomach. The pectin bacteriostatic microspheres exerted significant bacteriostatic effect on Escherichia coli and Staphylococcus aureus , indicating that they may be widely used in livestock feed as bacteriostatic agents. Graphical Abstract In order to improve the antibacterial effect of KDF in intestinal tract, W/O emulsion microcapsules were prepared by emulsification method. When Zeolite P was used as a drug-binding effector, KDF was uniformly dispersed in the pectin matrix, and the pectin bacteriostatic microspheres loaded with KDF were prepared using the emulsion cross-linking method. Moreover, the bacteriostatic microspheres possessed significant growth inhibitory effects on E. coli, S. aureus and Bacillus subtilis.
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FOOD HYDROCOLLOIDS
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2023
·
IF 10.7
Lin Li,Jia-Nan Yan,Bin Lai,Ce Wang,Jin-Jian Sun,Hai-Tao Wu
Chia seed gum was extracted using high-speed shearing technology and further purified via ethanol precipitation. The composition, structural and rheological properties of crude chia seed gum (CCSG) and purified chia seed gum (PCSG) were evaluated and further compared with twelve commercial polysaccharides (agar, xanthan gum , κ-/ι-/λ-carrageenan, guar gum, carboxymethyl cellulose , locust bean gum, pectin, acacia gum, sodium alginate and konjac gum). The extraction yields of CCSG were significantly improved from 1.80% to 6.25% by high-speed shearing for 2.0 min. Compared to CCSG (36.63% and 3.985 × 10 5 g/mol), PCSG had a higher total neutral sugar content (51.85%), lower molecular weight (1.718 × 10 5 g/mol) and looser surface morphology. Furthermore, PCSG and CCSG had different monosaccharide composition. However, both CCSG and PCSG exhibited pseudoplastic fluid behavior, but PCSG had higher viscosity and modulus values than CCSG at the same concentrations. Compared to commercial polysaccharides, PCSG had rheological characteristics similar to those of xanthan gum, with high viscoelasticity and good stability against temperature change. The relaxation time T 2 curves showed that CCSG and PCSG had better water-holding capacity than most commercial polysaccharides. This study provided theoretical guidance for the potential utilization of chia seed gum as a thickening and stabilizing agent in the food industry.
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INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2023
·
IF 5.8
Qiqi Sun,Jun Chen,Quan Zhao,Ziyun He,Lei Tang,Yuji Pu,Bin He
Ulcerative colitis (UC) as an important type of inflammatory bowel disease is a chronic disease characterized by intestinal dyshomeostasis. The UC treatment is challenged by the insufficiency of drug delivery and retention. Herein, we fabricated an intrarectal formulation of olsalazine (Olsa)-loaded hydrogel microspheres (LDKT/Olsa) with good bio-adhesiveness and reactive oxygen species (ROS)-scavenging ability to enhance drug retention and therapeutic effect. Low methoxy pectin-dopamine conjugate/konjac glucomannan composite hydrogel microspheres (LDKT) with a size ranging from 10 to 100 μm were prepared by using Zn 2+ and ROS-sensitive thioketal as crosslinkers. Upon intrarectal administration, the negatively charged and dopamine-functionalized hydrogel microspheres efficiently adhered to cationic surface of inflammatory mucosa, scavenging ROS and releasing Zn 2+ and Olsa for antibacterial and anti-inflammatory effects. In the dextran sodium sulfate (DSS)-induced mouse UC model, the microspheres significantly reduced the levels of colonic ROS and pro-inflammatory cytokines, improved gut mucosal barrier integrity, and remarkably relieved colitis . Overall, the LDKT microspheres are promising carriers to deliver drugs for UC treatment.
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Journal of Cleaner Production
·
2023
·
IF 11.1
Fei Zhu,Zhenqi Wang,Jingchun Huang,Wei Hu,Di Xie,Yu Qiao
Activated carbon derived from the organic solid waste is a promising material for ammonia removal. The components in the feedstock would affect the performance of activated carbon, but the detailed effect mechanism remains unknown. To reveal the correlations between the components of the feedstock and different ammonia adsorption styles of activated carbon, this study used pomelo peel to prepare ZnCl 2 -loaded activated carbon for ammonia adsorption via hydrothermal carbonization and KOH activation, focusing on the effect of the chemical components (cellulose, xylan , pectin, and lignin) on the physicochemical properties and adsorption performance of activated carbon. For raw activated carbon in moist conditions, ammonia was mainly adsorbed in the form of Lewis/Brønsted acid-base (37.8 mg/g), followed by the ZnCl 2 coordination compound and organic amine forms (22.3 mg/g). Lignin-added activated carbon had the highest adsorption capacity , 91.2 mg/g in moist conditions, followed by cellulose-added and xylan-added activated carbon. The presence of lignin was beneficial for the formation of the phenolic group of activated carbon (increased by 111.3%), which promoted ammonia adsorption in the form of Lewis acid-base, especially in dry condition. Cellulose and xylan enhanced the formation of carboxylic (i.e., increased by 53.0% for cellulose) and lactonic groups (i.e., increased by 73.4% for cellulose) of activated carbon, leading to an increase of ammonia adsorption in the form of Brønsted acid-base and organic amines in moist conditions. The results illustrated the detailed effect of the chemical components in the feedstock on the ammonia adsorption styles, which could guide the selection of appropriate materials to prepare activated carbon for highly efficient ammonia adsorption under various situation.
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BIOMACROMOLECULES
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2023
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IF 6.2
Jiaying Li,Yuji Pu,Sai Li,Bin He,Jianlin Chen
The pathogenesis of inflammatory bowel diseases (IBDs) including ulcerative colitis (UC) and Crohn’s disease is extremely cloudy. Maintaining the level of remission lesions in colitis is the default treatment attitude at present. Epithelial barrier restoration is considered as the same important strategy as colonic targeted drug delivery in UC treatment. In this paper, we developed a multilayer natural polysaccharide microsphere (pectin/chitosan/alginate) with pH and enzyme dual sensitivity to reduce the loss of medication in the upper digestive tract and preferentially adhere to exposed epithelial cells in colonic tissues by electrostatic forces for efficiently targeted UC treatment. Olsalazine as an inflammatory drug was efficiently loaded in the chitosan layer and realized a colonic pH-responsive drug release. Furthermore, the multilayer microspheres exhibited excellent capability in suppressing harmful flora and a bio-adhesion effect to extend the duration of local medicine. In the in vivo anti-colitis study, the downregulated levels of pro-inflammatory factors and the increase of tight junction protein indicated the excellent anti-inflammation effect of the olsalazine-loaded microspheres. In summary, these results showed that the multilayer natural polysaccharide microspheres could be a powerful candidate in the targeted drug delivery system for UC therapy.
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FOOD CHEMISTRY
·
2023
·
IF 8.8
Lechuan Wang,Mengzhuo Liu,Panpan Guo,Huajiang Zhang,Longwei Jiang,Ning Xia,Li Zheng,Qian Cui,Shihui Hua
Although protein-polysaccharide complexes have shown tremendous potential in stabilizing high internal phase Pickering emulsions (HIPPEs), it is unclear whether coacervates have the same potential to be used as effective Pickering stabilizers. In this study, HIPPEs were prepared by ovalbumin (OVA)-pectin (PE) coacervates during the transition from coacervates to complexes. The results showed that enhanced OVA-PE interactions significantly affected the wettability and surface-tension reduction ability of the OVA-PE coacervates. At pH 2, the coacervate-stabilized HIPPEs exhibited smaller oil droplet sizes (21.3±2.3 μm), tighter droplet packing, and finer solid-like structures through the bridging of droplets and the generation of stronger gel-like network structures to prevent coalescence and lipid oxidation. The gastrointestinal digestion results proved that the OVA-PE coacervates promoted lipid hydrolysis and improved the bioaccessibility (from 19.7±0.7% to 36.5±2%) of curcumin-loaded HIPPEs. Our work provides new ideas for the development of biopolymer particles as effective Pickering stabilizers in the food industry.
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ENVIRONMENT INTERNATIONAL
·
2019
·
IF 7.943
Jing Zhan,Yiran Liang,Donghui Liu,Xiaoran Ma,Peize Li,Wangjing Zhai,Zhiqiang Zhou,Peng Wang
Background The prevalence of obesity has raised global concerns. Environmental pollutants are one of the main causes of obesity. Many studies have demonstrated that dietary fiber could reduce obesity induced by high-fat diets, but whether environmental pollutant-induced obesity can be reversed is still unknown. Objectives This study aimed to investigate the effects of pectin on obesity induced by a typical environmental pollutant p,p'-dichlorodiphenyldichloroethylene (p,p′-DDE) and explore the underlying mechanism by which pectin reversed p,p′-DDE-induced obesity. Methods p,p′-DDE was used to induce obesity in C57BL/6J mice and pectin was supplied during and after cessation of p,p'-DDE exposure. Body and fat weight gain, plasma lipid profile and insulin resistance of mice were assessed. Gut microbiota composition and the levels of short-chain fatty acids (SCFAs) as well as the receptor proteins and hormones in the SCFAs-related signaling pathway were analyzed. Moreover, p,p′-DDE levels in various tissues of mice were detected. Results Pectin supplementation reversed body and fat weight gain, dyslipidemia, hyperglycemia and insulin resistance in p,p′-DDE-exposed mice. Furthermore, pectin apparently altered the p,p′-DDE-induced microbial composition and then promoted the levels of SCFAs in colonic feces as well as the expression of G-protein coupled receptors and the concentration of hormone peptide YY (PYY) and glucagon like peptide-1 (GLP-1). Pectin treatment also significantly reduced p,p′-DDE accumulation in mice tissues during p,p′-DDE exposure but did not change p,p′-DDE metabolism after termination of p,p′-DDE exposure. Conclusions Pectin had a good effect on reducing p,p′-DDE-induced obesity through regulating gut microbiota and provided a potential strategy for the treatment of environmental pollutant-caused health problems.
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CARBOHYDRATE POLYMERS
·
2018
·
IF 5.158
Hong-Bing Chen,Xin-Lei Li,Ming-Jun Chen,Yan-Rong He,Hai-Bo Zhao
Self-cross-linked aerogel based on pectin and melamine-formaldehyde resin (MF) was fabricated via freeze-drying method using water as solvent, where pectin is structural material meanwhile acting as acid to catalyse the cross-linking of MF. The cross-linking reaction easily occurs without additional additives, which can be significantly accelerated at elevated temperatures, with a critical value of about 55 °C. The obtained aerogel shows network microstructures as observed with SEM. With increasing pectin content, the aerogel shows significantly increased compressive modulus. The compressive modulus of M10Pe5 arrives 23.2 MPa, the specific modulus of which arrives 188 MPa cm 3 /g, while pure MF aerogel are too fragile to keep intact after freeze-dried. The resulting aerogel has good thermal stability, excellent water resistance (can be second dried with limited strength loss) and low flammability. This partially bio-based novel aerogel with impressive properties is promising in many applications.
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FOOD HYDROCOLLOIDS
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2019
·
IF 5.839
Yun-shan Wei,Kun Feng,Min-hua Zong,Hong Wu
To improve the bioavailability of lactoferrin (Lf) and achieve its controlled release in the intestinal tract, a pH-responsive composite system for the oral delivery of Lf was successfully developed for the first time. The negatively charged poly(lactide-co-glycolide) (PLGA)/poly(styrene-co-4-styrene-sulfonate) (PSS) nanoparticles (NPs) were firstly prepared by solvent displacement, and the resulting NPs were spherical in shape with diameter of around 120 nm and zeta potential of −50.7 mV. Then, the positively charged Lf was incubated with PLGA/PSS NPs followed by coating with pectin to improve the Lf adsorption efficiency. The Lf adsorption efficiency decreased with the increase of Lf concentration, but it was even as high as 80.5% when Lf concentration was 8.0 mg/mL. The pectin coated Lf-PLGA/PSS NPs were further incorporated into Eudragit S-100 (ES-100) micro-capsule by the self-assembly method and the obtained composite ES-100 micro-capsule was found to be spherical with an average diameter of 2.22 μ m. The in vitro release experiment showed that only a little Lf (<1%) was released from micro-capsule in simulated gastric fluid comparing with that of pectin coated Lf-PLGA/PSS NPs (30%), and around 82% Lf was released into the simulated intestinal tract in the following 8 h. The results of cytotoxicity test suggested that there was negligible toxicity of the composite micro-capsule against Caco-2 cells. The SDS-PAGE and CD spectrum analysis indicated that the micro-encapsulated Lf retained the integrity of its primary and secondary structure during the heating treatment process. Hence, the obtained Lf-loaded composite micro-capsule is promising in the oral delivery of Lf.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2019
·
IF 3.571
Laifeng Lu,Lifeng Ji,Qingqing Ma,Mingguan Yang,Shuhua Li,Qiong Tang,Liping Qiao,Fengjuan Li,Qingbin Guo,Changlu Wang
The acquisition of susceptibility to necrotrophy over the course of ripening is one of the critical factors limiting shelf life. In this study, phytopathology and molecular biology were employed to explore the roles of pectinase in fruit susceptibility and ripening. Solanum lycopersicum fruit softened dramatically from entirely green to 50% red, which was accompanied by a continuously high expressed SlPG2 gene. The necrotrophic fungus Botrytis cinerea further activated the expression of SlPGs and SlPMEs to accelerate cell wall disassembly, while most of the polygalacturonase inhibitor proteins encoding genes expression were postponed in ripe fruit following the pathogen attack. Pectin induced the antagonistic yeast to secrete pectinolytic enzymes to increase fruit resistance against gray mold. The activities of pathogenic pectinase of B. cinerea were correspondingly depressed in the pectin-inducible yeast enzyme elicited ripe fruit. These data suggest that pectinase is a molecular target for regulation of disease resistance during fruit ripening.
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JOURNAL OF INORGANIC BIOCHEMISTRY
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2018
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IF 3.063
Jiajing Diao,Feng Bai,Ying Wang,Qianqian Han,Xi Xu,Hongmei Zhang,Qiang Luo,Yanqing Wang
In this work, we designed polysaccharide-metal complex of dopamine-modified pectin linking ruthenium compound, which exhibited a certain inhibition specificity to human renal cell adenocarcinoma cell line 786-O. The chemical structure and physical properties of the polysaccharide-metal complex were well characterized by multiple analysis methods. The multi-spectral results revealed that pectin-dopamine have been successfully coordinated by ruthenium complex to form nano-conjugates, which self-assembled into relatively regular nanospheres of approximately 200 nm. The polysaccharide-metal complex has more amorphous and less viscosity than pectin, and cannot withstand as much strain as the pectin and pectin-dopamine systems. Pectin-dopamine can decrease the toxicity effect of Ru complex in normal cell line such as human normal renal epithelial cell line 293A.
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JOURNAL OF FOOD BIOCHEMISTRY
·
2018
·Vol. 43
·期 3
·e12741
·
IF 1.552
Si-Qi Yang,Xiao-Yan Dai,Xiao-Yi Wei,Qing Zhu,Tao Zhou
Co-immobilization of pectinase and glucoamylase onto sodium alginate/graphene oxide beads was achieved by N , N′ -dicyclohexylcarbodiimide/ N -hydroxysuccinimide as activating agent. The co-immobilized pectinase-glucoamylase (I-PG) prepared under optimal conditions (pH 4.0, 40°C and 35 min) possessed pectinase activity of 1,227.5 ± 36.5U/g and glucoamylase activity of 1,027.2 ± 29.2U/g, with activity recovery of 73.8% and 85.2%, respectively. Both pectinase and glucoamylase in I-PG possessed wider pH tolerance and superior thermal stability to those of their free counterparts. Reusability studies indicated that both enzymes in I-PG retained over 60% of initial activity after six times of reuse. Conditions for the hydrolysis of the pumpkin–hawthorn compound juice by I-PG were optimized using orthogonal experiments. After treatment with I-PG, light transmittance, soluble solids, and reducing sugar content in the resulting juice increased significantly, whereas soluble protein and pectin content decreased appreciably. Therefore, the use of I-PG provided an effective and feasible method for improving quality of the pumpkin–hawthorn juice. Practical applications In order to overcome the drawbacks of using free pectinase and glucoamylase, an effective method for the co-immobilization of these two enzymes onto sodium alginate/graphene oxide beads was developed. The co-immobilized pectinase/glucoamylase developed in this study could be applied in the clarification of juice rich in pectin and starch.
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