ANALYTICAL CHEMISTRY
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2025
·
IF 6.7
Junpeng Lv,Haibiao Lin,Xiaoxia Chen,Qiong Hu,Li Niu
Glycoproteins are of significant value to liquid biopsy of human diseases. Herein, we present a universal electrochemical platform for the amplified detection of glycoproteins, taking advantage of the glycan-matchmade multivalent decoration of enzyme labels for the enzymatic signal amplification. Briefly, the glycan-matchmade multivalent decoration involves two steps, i.e., the site-directed decoration of the phenylboronic acid-coated gold nanoparticles (PBA-AuNPs) to the cis-diol-containing glycans of glycoproteins and the subsequent decoration of enzyme labels via the affinity cross-linking between the glycan moieties of enzyme labels and the remaining PBA groups on the PBA-AuNP cross-linkers. As the glycan matchmaking-based strategy enables the decoration of each glycoprotein with multiple enzyme labels, this electrochemical platform exhibits a high sensitivity toward glycoprotein detection. Using alkaline phosphatase (ALP) as the proof-of-concept enzyme label in combination with the solid-state voltammetric stripping assay of the enzymatically deposited metallic silver, the detection limits at the pg mL–1 level have been obtained for the electrochemical aptamer-based detection of thrombin and prostate-specific antigen. Overall, this work illustrates an efficient and versatile strategy for the multivalent decoration of enzyme labels for electrochemical detection of glycoproteins at ultralow concentration levels, holding the desirable advantages of simplicity and cost-effectiveness over sandwich enzyme-linked immunosorbent assays.
研究领域:
生物化学
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS ES&T Engineering
·
2024
·
IF 7.4
Hui-Wen Huo,Xiao-Qiong Wu,Jia-Fang Xie,Ding Li,Quan-Bao Zhao,Yu-Ming Zheng
Chemical oxygen demand (COD) indicates the relative content of organics in water. The electrochemical measurement of COD is a promising ecofriendly alternative method for rapidly, automatically, and highly accurately determining the content of organics in water. However, the key anodes must possess high catalytic efficiency and long-term stability, be easy to prepare, and be free of heavy metal leaching. Therefore, in this study, a SnO2–Sb-based porous anode was prepared using simple coating and calcination. Because of its porous, conductive, and robust Ti felt/Pt (TF/Pt) substrate, the anode (TF/Pt/SnO2–Sb) possessed stable current responses that were highly correlated (R2 = 0.999) with COD measurements in the range of 20–6000 mg L–1. The detection limit was 3.03 mg L–1 (at a signal-to-noise ratio of 3), and the relative standard deviation was within 3.2% (n = 10), which is superior to those of the standard dichromate detection method. The further assembly of a flow-through system with the proposed anode exhibited a wider linear range of up to 8000 mg L–1 at a lower sample injection volume of 50 μL. These results suggest the application potential of the electrochemical method with the porous TF/Pt/SnO2–Sb anode as a reliable alternative for sustainable COD determination.
研究领域:
环境科学
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
·
2025
·
IF 3.3
Huang Yilu,Luo Huili,Zhao Jia,Xiang Yuhang
Electrochemical detection is an applicable rapid detection technology, but it is always limited by sample treatment and matrix interference. In this study, two gel/graphite paper electrodes were obtained by drop coating of sodium alginate gel and chitosan gel onto 0.1 mm graphite paper. Comparations of anti-interference performance and stability indicated that sodium alginate gel/graphite paper electrode (GGPBE) was the preferred term for establishing a rapid determination method for Pb in fruits. Based on the contents of organic acids and main metal ions in fruits, analysis and control of matrix interference were conducted. Organic acids, such as ascorbic acid, citric acid and quinic acid, caused greater interferences than metal ions. Mg(II) and Fe(III) were the notable factors of co-interference with organic acids. Matrix interference could be controlled in 3.92% by chemical oxidation using H 2 O 2 combined with HNO 3 ultrasonic extraction. The established linear range of Pb(II) was 0.1 ∽ 21.0 µg/L and LOD was 0.0274 µg/L under square wave anodic stripping voltammetry method. In the detection of 9 fruits, the contents of Pb were below 0.1 mg/kg. This work developed an easily prepared gel/graphite paper electrode and provided a rapid sample treating-detection method of lead in fruits.
研究领域:
分析化学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2013
·
IF 2.591
Xinchun Li,Zuanguang Chen,Fan Yang,Jianbin Pan,Yinbao Li
L -3,4-dihydroxyphenylalanine (L-DOPA) is a well-recognized therapeutic compound to Parkinson's disease. Tyrosine is a precursor for the biosynthesis of L-DOPA, both of which are widely found in traditional medicinal material, Mucuna pruriens . In this paper, we described a validated novel analytical method based on microchip capillary electrophoresis with pulsed electrochemical detection for the simultaneous measurement of L-DOPA and tyrosine in M . pruriens . This protocol adopted end-channel amperometric detection using platinum disk electrode on a homemade glass/polydimethylsiloxane electrophoresis microchip. The background buffer consisted of 10 mM borate (pH 9.5) and 0.02 mM cetyltrimethylammonium bromide, which can produce an effective resolution for the two analytes. In the optimal condition, sufficient electrophoretic separation and sensitive detection for the target analytes can be realized within 60 s. Both tyrosine and L-DOPA yielded linear response in the concentration range of 5.0–400 μM ( R 2 > 0.99), and the LOD were 0.79 and 1.1 μM, respectively. The accuracy and precision of the established method were favorable. The present method shows several merits such as facile apparatus, high speed, low cost and minimal pollution, and provides a means for the pharmacologically active ingredients assay in M . pruriens .
研究领域:
分析化学
·
药学
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植物化学
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2011
·
IF 3.37
Cheng Fang,Jishan He,Zhencheng Chen
A disposable amperometric biosensor for detecting the total cholesterol was fabricated which comprises a sensing electrode and a reference electrode in simultaneous contact with an integrated reagent layer. The integrated reagent layer formed by coating a working ink containing cholesterol esterase, cholesterol dehydrogenase, coenzyme , redox mediator , surfactant, stabilizer, filler and at least one aqueous thickening agent . The biosensor showed the linearity for 50–500 mg/dL cholesterol acetate. The minimum detection limit of the cholesterol was 50 mg/dL. The effects of temperature and the stability of immobilized enzymes were also studied. A good correlation was found among cholesterol values obtained by commercial colorimetric test strip and clinical/laboratory methods. The biosensor showed an acceptable reproducibility, good stability and low interferences.
研究领域:
生物医学工程
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分析化学
✓ 已验证
DOI
高影响因子
IONICS
·
2019
·Vol. 25
·期 12
·6051-6059
·
IF 2.289
Zhang Yanqing,Huang Yanli,Feng Chuanqi,Zhang Yimin,Wu Huimin
In this work, Co 3 O 4 was synthesized by a simple hydrothermal and subsequent calcination treatment for electrochemical detection of H 2 O 2 . In order to improve the electrochemical performance of Co 3 O 4 , it is compounded with the carbon materials derived from cypress leaves, loofah sponge, and pine needles. The electrochemical tests suggested that Co 3 O 4 /loofah sponge-derived carbon showed higher electrochemical activity towards H 2 O 2 than the other two composites. The sensor based on Co 3 O 4 /loofah sponge-derived carbon exhibited a broad linear range from 5.00 μM to 11.40 mM, with a sensitivity of 47.83 μA mM −1 cm −2 and a detection limit of 1.50 μM ( S / N = 3). Thus, this novel nonenzymatic sensor had potential application in H 2 O 2 detection.
研究领域:
材料科学
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分析化学
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Weiwei Mao,Haiping He,Zhizhen Ye,Jingyun Huang
A facile and one-step transformation towards graphene using nickel foams as the substrate into a contact between semiconducting graphene (SG) and metal is developed for fabricating a SG/Ni Schottky junction. Graphene quantum dots were cut away via the electrochemical redox method and porous three-dimensional graphene with a bandgap was generated due to the abundant defects and dangling bonds . The constructed Schottky junction is the inner drives of accelerating charge transfer and thus results in significantly enhanced electrocatalytic effect. Bio-inspired porous graphene integrates micro-nano pores and functional groups, which greatly benefits to molecules separation and specific electrocatalysis . Macroscopic pores and numerous nanopores serve as “highways” for the rapid mass transport. Massive defects and rich dangling bonds act as “perceptrons” to reflect the charge distribution change. As a successful demonstration, the fabricated SG/Ni Schottky junction exhibits appreciable electro-oxidation towards H 2 O 2 with an ultrahigh sensitivity of 4838 μA mM −1 cm -2 and an ultralow detection limit of 20 nM. This study puts forward an easy and practicable strategy to construct a Schottky junction, which is promisingly applied in other electrochemical research.
研究领域:
材料科学
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电化学
·
生物医学工程
✓ 已验证
DOI
高影响因子
Research
·
2022
·
IF 11.036
Liangfen Cheng,Fuhan Yang,Longfei Tang,Lelin Qian,Xu Chen,Feng Guan,Juan Zhang,Genxi Li
Evaluating tumor development is of great importance for clinic treatment and therapy. It has been known that the amounts of sialic acids on tumor cell membrane surface are closely associated with the degree of cancerization of the cell. So, in this work, cellular interface supported CRISPR/Cas trans-cleavage has been explored for electrochemical simultaneous detection of two types of sialic acids, i.e., N-glycolylneuraminic acid (Neu5Gc) and N-acetylneuraminic acid (Neu5Ac). Specifically, PbS quantum dot-labeled DNA modified by Neu5Gc antibody is prepared to specifically recognize Neu5Gc on the cell surface, followed by the binding of Neu5Ac through our fabricated CdS quantum dot-labeled DNA modified by Sambucus nigra agglutinin. Subsequently, the activated Cas12a indiscriminately cleaves DNA, resulting in the release of PbS and CdS quantum dots, both of which can be simultaneously detected by anodic stripping voltammetry. Consequently, Neu5Gc and Neu5Ac on cell surface can be quantitatively analyzed with the lowest detection limits of 1.12 cells/mL and 1.25 cells/mL, respectively. Therefore, a ratiometric electrochemical method can be constructed for kinetic study of the expression and hydrolysis of Neu5Gc and Neu5Ac on cell surface, which can be further used as a tool to identify bladder cancer cells at different development stages. Our method to evaluate tumor development is simple and easy to be operated, so it can be potentially applied for the detection of tumor occurrence and development in the future.
研究领域:
生物医学工程
·
分子生物学
·
肿瘤学
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Jing Zhang,Xiaojian Xu,Lu Chen
This work constructed an ultrasensitive electrochemical bisphenol A (BPA) sensor using hierarchical Ce-metal-organic framework (Ce-MOF) modified with cetyltrimethylammonium bromide (CTAB) as a sensing platform. Ce-MOF had been successfully prepared and conveniently modified with a cationic surfactant (CTAB) via the electrostatic interaction. Ce-MOF and CTAB/Ce-MOF were characterized by scanning electron microscopy, X-ray diffraction, infrared spectrum, thermogravimetric analysis and zeta potentials analysis. CTAB/Ce-MOF suspension was dropped onto glassy carbon electrode (GCE) surface to fabricate CTAB/Ce-MOF/GCE. Combined with preconcentration of BPA of the long alkane chain in CTAB via hydrophobic interaction and electrocatalytic activity of Ce-MOF, the dual amplification made CTAB/Ce-MOF/GCE exhibit high electrochemical response toward the oxidation of BPA, and an electrochemical BPA sensor was constructed based on CTAB/Ce-MOF/GCE by differential pulse voltammetry. The designed BPA sensor had a wide linear range from 0.005 to 50 μmol L −1 and a low detection limit of 2.0 nmol L −1 (S/N = 3). The proposed sensor had excellent reproducibility, stability and anti-interference, and was successfully employed for BPA detection in real samples. This convenient and sensitive sensor proved to be a promising and reliable tool for detecting BPA in food and environmental pollution.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
ANALYST
·
2012
·Vol. 138
·期 4
·1226-1231
·
IF 4.23
Shanshan Xie,Fang Wang,Zilin Chen
Jasmonic acid (JA), a type of plant hormone, is widely distributed in a variety of higher plants at very low concentrations, usually several nanograms per gram (ng g−1) fresh weight of the plant tissue. The determination of endogenous JA is challenging work. The typical electrochemical oxidation behavior of JA could only be achieved under extreme conditions such as strongly acidic medium (H2SO4) and high applied working potential (1.4–1.6 V), which cannot be used in the electrochemical detection for liquid chromatography (LC). To realize electrochemical detection for LC separation, a mild supporting electrolyte for JA oxidation is required, as the supporting electrolyte solution also serves as the mobile phase of LC. Thus, a novel electrochemical derivatization with dopamine (DA) has been developed and successfully applied to the analysis of endogenous JA in wintersweet flowers and rice florets by liquid chromatography coupled with electrochemical detection (HPLC-ECD). Under optimized experimental conditions including a detection potential of +0.90 V, and 0.04 mol L−1 acetate buffer solution (pH 5.07) : acetonitrile (67 : 33, v/v) as the mobile phase, the contents of JA in wintersweet flowers and rice florets were respectively determined to be 7.86 μg g−1 and 308 ng g−1, consulting the linear relationship between the peak area of JA–DA derivatives and the standard JA concentration (1.0 × 10−7 mol L−1 to 2.0 × 10−5 mol L−1, R = 0.9986) with a detection limit of 5.0 × 10−8 mol L−1 (S/N = 3). The results were consistent with those by LC-UV and LC-MS methods in our group, indicating that this novel pre-column electrochemical derivatization method is feasible.
研究领域:
植物学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ELECTROANALYSIS
·
2016
·Vol. 28
·期 9
·2029-2036
·
IF 2.471
Yuanping Peng,Yangjuan Wang,Yijun Li,Xiwen He
Multi-walled carbon nanotubes (MWCNTs) functionalized with polyethylenimine (PEI) were synthesized and characterized by dispersibility, field-emission scanning electron microscope (FE-SEM), FT-IR and thermogravimetric Analyzer (TGA). The glassy carbon electrodes modified by MWCNT-PEI composite were used for sensitive and selective detection of chromium (VI). A linear response was obtained over a wide range of Cr(VI) concentrations (0.002–20 µmol L −1 ) with the detection limit of 0.0006 µmol L −1 (S/N=3). The proposed electrodes were used successfully for Cr(VI) detection in three real water samples.
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
Advanced Materials Interfaces
·
2022
·Vol. 10
·期 1
·2200738
·
IF 6.389
Bo Wu,Minzhang Li,Rajendran Ramachandran,Gaoqiang Niu,Mingxiang Zhang,Changhui Zhao,Zongxiang Xu,Fei Wang
Dopamine (DA) and uric acid (UA) are biomolecules of great consideration that coexist in human body. It is essential and challenging to detect them simultaneously with high sensitivity. This paper reports a novel electrochemical biosensor based on the nanocomposites of cobalt phthalocyanine (CoPc) anchored with graphene quantum dots (GQDs) for DA and UA detection. The GQDs incorporation on CoPc nanorods with partial end stretching significantly enhances the electrocatalytic activity and promotes the electron transfer rate. Low detection limits of 21 n m for DA and 145 n m for UA have been successfully achieved for the fabricated CoPc/GQDs biosensor. The density-functional theory proves the enhanced conductivity of CoPc/GQDs and the biomolecule's interaction with the electrode surface. Furthermore, the proposed sensor shows remarkable reproducibility, repeatability, and stability. The fabricated sensor is applied for the simultaneous detections of DA and UA in human urine samples. The excellent performance suggests that the CoPc/GQDs could be a promising and potential candidate for electrochemical sensors to detect DA and UA selectively.
研究领域:
生物传感器
·
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）