ACS Applied Nano Materials
·
2024
·
IF 5.3
Ke Ma,Meng Yin,Xiao-Xue Wang,Chen-Yu Wang,Ke-Zheng Chen,Sheng-Lin Qiao
There is an urgent need for efficient and convenient methods to detect SARS-CoV-2 as the global pandemic continues. Currently, detection of COVID-19 relies primarily on reverse transcription PCR (RT-PCR), a laboratory-based method that is time-consuming. To tackle this challenge, the study proposes an immediate high-throughput detection platform for SARS-CoV-2. The platform uses a gold nanoenzymatic colorimetric assay that focuses on the specific detection of 3CLpro in SARS-CoV-2. The platform has been extensively characterized for stability, sensitivity, detection range, and response time. The colorimetric detection method not only provides easy-to-read results but also allows color readings to be taken using a smartphone, with accuracy comparable to that of large instruments such as a microplate reader. The innovation of this technology lies in the ability to achieve instant high-throughput detection, which makes rapid detection of SARS-CoV-2 feasible. The proposed detection platform has a wide range of potential applications, particularly in the field of virus detection, providing an efficient and reliable solution for managing current pandemics and future disease prevention and control.
研究领域:
病毒学
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医学检测
·
纳米生物学
✓ 已验证
产品关联
DOI
高影响因子
MOLECULES
·
2019
·Vol. 24
·期 13
·2442
·
IF 3.06
Pengfei Zhuang,Peng Zhang,Kuo Li,Beena Kumari,Dan Li,Xifan Mei
Metal nanomaterials have been reported as effective absorbents for the removal of pollutants in the water system, but the release of ions from these nanomaterials brings another concern. Herein, silver nanoclusters (AgNCs) were encapsulated in porous metal-organic frameworks of ZIF-8 (MOF-AgNCs). Compared to AgNCs, the release of Ag+significantly decreases from MOF-AgNCs, indicating that the product presents a lower threat to the environment. The MOF-AgNCs were employed for the rapid removal of heavy metals, such as Pb2+and Mn2+, from water. The mechanism and removal efficiencies were investigated.Keywords:nanoclusters;metal-organic frameworks;heavy metal ions
研究领域:
环境科学
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
Catalysis Science & Technology
·
2018
·Vol. 8
·期 24
·6384-6395
·
IF 5.365
Zhiyang Zhang,Yanle Li,Jing Gu,Liping Ding,Nianhua Xue,Luming Peng,Xuefeng Guo,Yan Zhu,Jing Ma,Weiping Ding
The electrostatic field of a dielectric cavity is powerful for modulating many aspects of confined metal clusters. Here we report that the catalytic properties of Ptn clusters confined in X-zeolite cages are strongly tuned by altering the zeolitic field, which is achieved by using different cations (Na+, Cs+, Ca2+ or La3+) to balance the negative framework of the zeolite. Compared with Ptn@LaX, for example, Ptn@CsX shows a surprising ∼5500% enhancement in TOF value for phenol hydrogenation to cyclohexanol. Electrostatic field modulation leads to gradual changes of electronic properties such as the Fermi levels/d-band centers of the Ptn clusters, as revealed by DFT calculations and experimental characterizations. The decreases in Fermi levels of the Ptn clusters benefit the hydrogenation of –CCH groups (phenylacetylene and acetylene) and –NO2 groups (nitrobenzene, o-nitroanisole and o-chloronitrobenzene) but are detrimental for aromatic ring hydrogenation (phenol). These findings are important for the design of high performance catalysts.
研究领域:
化学
·
材料科学
✓ 已验证
DOI
高影响因子
Analytical Methods
·
2019
·Vol. 11
·期 28
·3584-3589
·
IF 2.378
Yanqiong Lai,Xia Teng,Yanli Zhang,Hongbin Wang,Pengfei Pang,Chun Yang,Colin J. Barrow,Wenrong Yang
In this study, we developed a simple, sensitive, low-cost and label-free method to detect rutin by using double-stranded DNA-templated copper nanoclusters (dsDNA-CuNCs) as a fluorescent probe. The dsDNA-template can facilitate the formation of highly fluorescent CuNCs with a maximum emission wavelength of 575 nm. In the dsDNA-CuNC fluorescent probe, rutin was intercalated into the structure of dsDNA via hydrogen bonding and electrostatic interactions. The fluorescence of dsDNA-CuNCs was quenched considerably due to the electron transfer from CuNCs to rutin. This decrease in the fluorescence intensity of dsDNA-CuNCs can be used to monitor the concentration of rutin from 0.6 to 400 μM with a detection limit of 0.12 μM (S/N = 3). Compared with the previous reports, this method does not require any complex DNA sequence design, fluorescent dye label and sophisticated experimental techniques. Moreover, the proposed sensing system could be applicable for the detection of rutin in pharmaceutical samples.
研究领域:
生物化学
·
分析化学
·
药物分析
✓ 已验证
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2020
·
IF 8.758
Xue Zhao,Ziqiong Yang,Artem V. Kuklin,Glib V. Baryshnikov,Hans Ågren,Xiaohai Zhou,Haibo Zhang
Currently, the development of stable electrochemical nitrogen reduction reaction (ENRR) catalysts with high N2 conversion activity and low cost to instead of the traditional Haber-Bosch ammonia production process of high-energy consumption remains a great challenge for researchers. Here, we have immobilized reductive closo-[B12H11]− boron clusters on a carbon nanotubes (CNT) surface and have successfully prepared a novel Au-CNT catalyst with extraordinary ENRR activity after adding HAuCl4 to the CNT-[B12H11]− precursor. The excellent properties of ammonia yield (57.7 μg h–1 cm–2) and Faradaic efficiency (11.97%) make it possible to achieve using this Au-CNT catalyst in large-scale industrial production of ammonia. Furthermore, its outstanding cyclic stability and long-term tolerability performance make it one of the most cost-effective catalysts to date. Here, by means of density functional theory we disclose the associative mechanism of N2-to-NH3 conversion on the Au(111) surface, providing visual theoretical support for the experimental results.
研究领域:
材料科学
·
化学工程
·
理论化学
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DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2021
·Vol. 413
·期 6
·1639-1649
·
IF 4.157
Fereja Shemsu Ligani,Fang Zhongying,Li Ping,Guo Jinhan,Fereja Tadesse Haile,Chen Wei
Balanced level of hemin in the body is fundamentally important for normal human organ function. Therefore, environmentally benign, stable, and fluorescent metal nanoclusters (NCs) for selective and sensitive detection of hemin have been investigated and reported. Herein, highly orange red emissive gold NCs are successfully synthesized using glutathione as a reducing and stabilizing agent (GSH-Au NCs). The clusters are characterized using various techniques like Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), UV-vis spectroscopy, and fluorescence spectrometer. The fluorescence intensity of as-synthesized Au NCs strongly quenched upon addition of different concentrations of hemin. The decrease in fluorescence intensity of GSH-Au NCs has been applied for determination of hemin concentration in the linear range from 1 to 25 nM with a low limit of detection (LOD) of 0.43 nM. The method was also successfully applied for quantification of hemin in human serum sample. In view of this reality, the system can be considered as a possible strategy and excellent platform for determination of hemin in various areas of application. Graphical abstract
研究领域:
生物化学
·
医学检测
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2020
·
IF 5.339
Shanliang Song,Yue Zhao,Yang Li,Xudong Yang,Dong Wang,Zhuoqi Wen,Minghui Yang,Quan Lin
The profiling of bacterial metabolism is of great significance in practical applications. Therefore, the development of ultrasensitive and highly selective probe for bacterial metabolism detection and imaging is extremely desirable. Herein, a novel dual-emission pH-response bacterial metabolism detection and imaging probe is successfully developed. This probe consists of large-sized and easily separated SiO 2 microspheres , copper nanoclusters (Cu NCs) with red emission, and carbon dots (CDs) with blue emission through in-situ self-assembly. In this system, the fluorescence of Cu NCs is sensitive to pH change due to their obvious aggregation-induced emission enhancement (AIEE) property, while the blue fluorescence of CDs remained almost stable. Therefore, red fluorescence and blue fluorescence are compounded with different fluorescence intensity at different pH values, and their fluorescence ratio is also different. By observation of composite fluorescence color, the visual colorimetric pH detection can be realized with the change of pH value of 0.2 units. Utilizing this system, we are able to detect bacterial metabolism with high signal-to-noise ratio, and it can also be used for bacterial metabolic imaging. Therefore, the pH-responsive Cu NCs-based dual-emission ratiometric fluorescent probe we constructed can provide new ideas for bacterial detection, antimicrobial sterilization, and biological imaging.
研究领域:
生物化学
·
微生物学
·
生物成像
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Chinese Journal of Natural Medicines
·
2020
·
IF 2.014
Yong-Jiang WANG,Li WANG,Xin HE,Dong-Dong XU,Jun TANG,Ya-Tuan MA,Yi-Jun YAN,Yu-Cheng GU,Jing YANG,Sheng-Xiong HUANG
Inthomycins are polyketide antibiotics which contain a terminal carboxamide group and a triene chain. Inthomycin B ( 1 ) and its two new analogues 2 and 3 were isolated from the crude extract of Streptomyces pactum L8. Identification of the gene cluster for inthomycin biosynthesis as well as the 15 N -labeled glycine incorporation into inthomycins are described. Combined with the gene deletion of the rare P450 domain in the NRPS module, a formation mechanism of carboxamide moiety in inthomycins was proposed via an oxidative release of the assembly chain assisted by the P450 domain.
研究领域:
微生物学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Nano
·
2021
·
IF 15.881
Jinglin Shen,Qianwen Xiao,Panpan Sun,Jin Feng,Xia Xin,You Yu,Wei Qi
The self-assembly of chiral metal nanoclusters into supramolecular chiral aggregates is of interest for developing advanced materials. Herein, we manipulated the self-assembly of Au nanoclusters modified by l-/d-cysteine (l-/d-AuNCs) into ordered microstructures featuring enhanced phosphorescence and optical activities. The formation of these aggregates was driven by synergistic effect of coordination and electrostatic interactions assisted by Cd2+/H+. Detailed structural characterization and theoretical studies confirmed that the compact aggregation structures are essential for the emission enhancement and the chirality amplification of l-/d-AuNCs. Interestingly, upon the formation of microflowers, the emission lifetime was prolonged to 3.34 ms with a switch from fluorescence to phosphorescence induced by aurophilic Au(I)···Au(I) interactions and intensive ligand-to-metal charge transfer (LMCT). Moreover, both the CD and photoluminescence (PL) signals of the microflowers exhibited pH-responsiveness. This dual-mode sensitive platform could be developed as a pH sensor with improved accuracy. Additionally, the pH-responsive photoluminescence ON/OFF switch of the microflowers could be employed for reliable information encryption and decryption. This study provides useful ideas for regulating the self-assembly of nanoclusters to generate desired photophysical properties with potential applications.
研究领域:
材料科学
·
光学
·
化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYST
·
2020
·Vol. 145
·期 15
·5273-5279
·
IF 3.978
Luping Feng,Jiani Yang,Sheng Zhang,Lixiang Zhang,Xi Chen,Pan Li,Yuan Gao,Shujing Xie,Yun Zhang,Hua Wang
A capillary-based fluorimetric analysis method was developed for probing glucose (Glu) in blood using Glu oxidase-anchored gold nanoclusters (GOD–AuNCs) and the ZIF-8 matrix. AuNCs were attached with GOD to be further encapsulated into the ZIF-8 matrix through the protein-mediated formation route. The resulting GOD–AuNCs@ZIF-8 nanocomposites could present the AuNC-improved catalysis of GOD and ZIF-8-improved environmental stability. The ZIF-8-enhanced fluorescence intensity of AuNCs could also be expected. Moreover, a capillary-based fluorometric platform was constructed for sensing Glu by coating the capillaries first with GOD–AuNCs and then the ZIF-8 matrix. Herein, Glu was introduced through the self-driven sampling to trigger the GOD-catalyzed production of hydrogen peroxide, which could induce the fluorescence quenching rationally depending on the Glu concentrations. The developed fluorimetric method could allow for the rapid and simple detection of Glu with the concentrations linearly ranging from 5.0 μM to 2.5 mM. Besides, the feasibility of practical applications was demonstrated by the evaluation of Glu in blood showing the recoveries of 96.2%–103.4%. Importantly, the proposed design of the capillary-based fluorimetric platform by the synergetic combination of catalysis-specific recognition and fluorescence signaling may open a new door toward extensive applications in the biological sensing, catalysis, and imaging fields.
研究领域:
生物传感
·
分析化学
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Ke Huang,Rui Dai,Wenqing Deng,Shengjie Guo,Han Deng,Yao Wei,Fuling Zhou,Ying Long,Jun Li,Xin Yuan,Xiaoli Xiong
A novel visual detection method was developed for the determination of zinc (Zn) in whole blood and cells using gold nanoclusters (AuNCs) immobilized paper. The detection of Zn was based on a headspace-solid phase extraction (HS-SPE), visible fluorescence from the AuNCs coated paper was quenched by ZnH 2 generated from hydride generation (HG). The potential mechanism was investigated by using X-ray photoelectron spectroscopy (XPS) and Density Functional Theory (DFT). The selectivity was significantly increased because the zinc hydride was effectively separated from sample matrices by hydride generation. A limit of detection (LOD) of 3 μg L −1 Zn 2+ and a relative standard deviation (RSD, n = 7) of 2% at a concentration of 50 μg L −1 of Zn 2+ were obtained when using a commercial fluorescence spectrophotometer as the detector. In visual detection, as low as 20 μg L −1 of Zn 2+ in biological samples can be easily discriminated from the blank with the naked eye. The proposed method retains several unique advantages, including straightforward, rapid, affordable and equipment-free for visual detection.
研究领域:
分析化学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2017
·
IF 3.582
Zongxiong Xu,Hao Fu,Tian Wen,Lei Zhang,Jian Zhang
Titanium-oxo clusters (TOCs) are important model complexes and potential precise single-source precursors of TiO 2 materials. We report here a series of TiO 2 samples derived from TOCs with the same wheel-like Ti 8 O 8 clustercore but different stabilizing ligands. Our results demonstrate that the ligands on TOCs not only influence the phase-transition during themolysis, but more importantly tune the photocatalytic H 2 evolution activities of the obtained TiO 2 materials. Through 500 °C calcination , the nanosized Ti 8 O 8 cluster stabilized by organic benzoate ligands gives rise to a TiO 2 sample with H 2 production rate of 611 μ mol/h/g. When the stabilizing ligands are changed to inorganic SO 4 2− , a significant improvement of H 2 evolution activity has been achieved, resulting in a 7-times higher value of 4527 μ mol/h/g. This work provides a new strategy to produce TiO 2 with enhanced and tunable photocatalytic activities from titanium-oxo cluster precursors.
研究领域:
材料科学
·
化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）